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All Journal Agrikultura Biotropika AGRICOLA Jurnal Biologi Tropis Jurnal Agrotek Tropika JURNAL PERTANIAN PRESISI (JOURNAL OF PRECISION AGRICULTURE) Jurnal Agrotropika Biofarm : Jurnal Ilmiah Pertanian Jurnal Pengabdian Dharma Wacana Organisms: Journal of Biosciences
Sari, Resti Puspa Kartika
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

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Efektivitas Asam Humat dan Plant Growth Promoting Rhizobacteria terhadap Pertumbuhan Bibit Kakao pada Media Tanam Subsoil Sari, Resti Puspa Kartika; Utoyo, Bambang; Permatasari, Nindy; Airlangga, Tandaditya Ariefandra; Nurmayanti, Sri; Lesmana, Adit Indra
Agrikultura Vol 37, No 1 (2026): April, 2026
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v37i1.68757

Abstract

Media tanam topsoil untuk pembibitan kakao semakin terbatas dan sebagai alternatif dapat digunakan tanah subsoil, tetapi kesuburannya ditingkatkan dengan penambahan asam humat. Selain itu, untuk mengurangi penggunaan pupuk anorganik pada tahap pembibitan dapat digunakan pupuk hayati Plant Growth Promoting Rizhobacteria (PGPR). Penelitian ini bertujuan untuk memperoleh konsentrasi asam humat dan PGPR terbaik, serta interaksi keduanya dalam mengoptimalkan pertumbuhan bibit kakao di media tanam subsoil. Penelitian dilaksanakan di lahan kebun bibit tanaman perkebunan, Laboratorium Pengelolaan Tanaman Perkebunan, Politeknik Negeri Lampung. Penelitian menggunakan Rancangan Acak Kelompok (RAK) pola faktorial  dengan tiga ulangan. Faktor pertama adalah konsentrasi asam humat (tiga taraf: 0; 5; dan 7,5 g/L). Faktor kedua adalah konsentrasi PGPR (empat taraf: 0; 25; 50; dan 75 mL/L). Data dianalisis menggunakan analisis sidik ragam, dan dilanjutkan dengan uji lanjut jarak berganda Duncan (DMRT) pada taraf nyata 5%.  Hasil penelitian menunjukkan bahwa terdapat interaksi antara asam humat dengan PGPR pada tingkat kehijauan daun dan bobot kering tajuk, dengan konsentrasi paling optimum pada perlakuan 0 g/L asam humat + 25 mL/L PGPR dan 5 g/L asam humat + 50 mL/L PGPR.  Pemberian asam humat secara mandiri berpengaruh pada bobot kering akar dengan perlakuan terbaik pada konsentrasi 0 g/L dan  5 g/L asam humat, dan pada luas daun untuk konsentrasi 0 g/L asam humat. Pemberian PGPR secara mandiri berpengaruh pada bobot kering akar, luas daun, dan jumlah daun 30 HSA. Konsentrasi aplikasi PGPR terbaik untuk bobot kering akar dan luas daun adalah 75 mL/L, sedangkan untuk jumlah daun 30 HSA adalah 50 mL/L.
Genetic Research Trends in Caffeine Biosynthesis of Robusta Coffee (Coffea canephora) Permatasari, Nindy; Sari, Sismita; Nurmayanti, Sri; Fauziah, Lu'lu' Kholidah; Sari, Resti Puspa Kartika; Hardani, Maisuri; Aliyah, Siti Hamidatul; Priyambodo, Priyambodo
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11738

Abstract

Research on caffeine biosynthesis in Coffea canephora has expanded across multiple disciplines, yet its global genetic research trends remain insufficiently mapped. This study aimed to analyze the development and thematic structure of genetic research on caffeine biosynthesis in C. canephora using a bibliometric approach. Data were retrieved from the Scopus database through keyword-based sampling and filtered based on relevance to genetic and biosynthetic aspects. Bibliometric analyses were conducted to examine publication trends, authorship patterns, country contributions, and keyword co-occurrence networks. The results show a significant increase in publications since the early 2000s, with dominant contributions from the United States, Brazil, and China. Keyword mapping reveals four major research clusters, including plant metabolism, clinical and nutritional studies, experimental models, and genetic variability. However, studies explicitly focusing on the genetic regulation of caffeine biosynthesis, particularly involving N-methyltransferase genes, remain relatively limited compared to broader multidisciplinary themes.These findings indicate that caffeine research in C. canephora is highly interdisciplinary but still lacks a strong emphasis on genetic mechanisms, highlighting the need for more targeted molecular and genomic studies to support coffee improvement and functional trait development.
Cytogenetic Evolution and Research Trends in Coffea spp.: Integrating Bibliometric Analysis with Karyotype Evidence Permatasari, Nindy; Fauziah, Lu’lu’ Kholidah; Sari, Resti Puspa Kartika; Hardani, Maisuri; ‘Aliyah, Siti Hamidatul; Siburian, Nora Vetty Vera; Saputra, Deni Tri; Nindita, Miftah Amalia; Rohidayanti, Iis; Aprilintan, Ayuhana; Syahputra, M. Damar; Kumalasari, Yuyun; Saputra, Rama Arsalta Bara; Alansyah, Rio Duta; Rahma, Chanda Rizkia; Simamora, Tomuan Harry Brossy; Rani, Zahra Fania Qud’; Priyambodo
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/e84tv628

Abstract

This study investigates cytogenetic evolution and research trends in Coffea spp. by integrating bibliometric analysis with karyotype-based evidence. Despite the rapid advancement of genomic research in Coffea spp., the integration of cytogenetic perspectives into broader research trends remains limited. Bibliometric data were retrieved from the Scopus database, covering publications from 1937 to 2026, resulting in 383 articles and reviews analyzed using Biblioshiny through thematic mapping and thematic evolution approaches. The results indicate that coffee genetic research has progressively shifted toward molecular and genomic studies, particularly those related to genetic variation, genome-wide association studies, and high-throughput analytical methods. In contrast, cytogenetic themes, including chromosome organization, karyotype variation, and polyploidization, remain comparatively underrepresented within the broader research landscape. Thematic evolution analysis further reveals a transition from foundational genetic studies to advanced genomic frameworks over time. Cytogenetic synthesis highlights major differences among key coffee species, with Coffea arabica characterized as an allotetraploid species (2n = 44), whereas C. canephora and C. liberica exhibit diploid chromosome complements (2n = 22). These findings demonstrate that chromosome-level perspectives remain insufficiently integrated into contemporary genomic research despite their importance in understanding genome evolution and species differentiation. By combining bibliometric trends with cytogenetic evidence, this study provides a more comprehensive framework for interpreting coffee genome organization and emphasizes the importance of integrating structural and molecular approaches in future coffee research and breeding programs.
Co-Authors Airlangga, Tandaditya Ariefandra Alansyah, Rio Duta Albertus Sudirman, Albertus Aprilintan, Ayuhana Ashadi, Ibnu Bambang Utoyo Dewi Riniarti Dulbari, Dulbari Fauziah, Lu'lu' Kholidah Fauziah, Lu’lu’ Kholidah Guna, M. Mulya Adi Hidayat Pujisiswanto Kresna Shifa Usodri Kumalasari, Yuyun Lesmana, Adit Indra Mahhendra, Bawon Maisuri Hardani, Maisuri Muhammad Tahir Nindita, Miftah Amalia Nurmayanti, Sri Permatasari, Nindy Priyadi Priyadi Priyambodo Priyambodo Priyambodo Rahma, Chanda Rizkia Rahmadi, Rizky Rani, Zahra Fania Qud’ Rochman, Fajar Rohidayanti, Iis Saputra, Deni Tri Saputra, Rama Arsalta Bara Siburian, Nora Vetty Vera Simamora, Tomuan Harry Brossy Sismita Sari, Sismita Syahputra, M. Damar Widiyani, Dimas Prakoswo Widyani, Dimas Prakoso Wiwik Indrawati, Wiwik ‘Aliyah, Siti Hamidatul ‘Aliyah, Siti Hamidatul