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Evaluasi Jumlah Monosit, Jumlah Limfosit dan Kadar Anti Streptolysin-O pada Pasien Tonsilitis Anak Hanny Dwi Indah Nurcahyani; Dita Pratiwi Kusuma Wardani; Arif Mulyanto; Kurnia Rhitma Dhanti
Jurnal Biodiversitas dan Bioteknologi Vol 2 No 2 (2025): Biodiverse: Jurnal Biodiversitas dan Bioteknologi
Publisher : Jurnal Biodiversitas dan Bioteknologi

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Abstract

Tonsillitis is the inflammation of the tonsils that can occur in children, caused by the bacteria Streptococcus haemolyticus and Streptococcus pyogenes. Poor oral hygiene, physical exhaustion, and certain types of food are other risk factors for chronic tonsillitis. When bacteria enter the tonsil tissue, monocytes differentiate into macrophages that destroy foreign objects. Activated lymphocytes differentiate into plasma cells that produce ASO antibodies. This study aims to determine the relationship between the number of monocytes and lymphocytes and Anti-Streptolysin-O levels in pediatric tonsillitis patients. This study was an observational analytical study with a cross-sectional design conducted in July-August 2025 at Dr. R. Goeteng Taroenadibrata Purbalingga Regional General Hospital by measuring the number of monocytes and lymphocytes using a hematology analyzer and Anti-Streptolysin-O levels using the latex agglutination method. The sample size for this study was 32 pediatric tonsillitis patients using accidental sampling. The relationship between monocyte and lymphocyte counts and ASO levels was analyzed using Spearman's rho correlation test. The results of the Spearman's rho correlation test showed that there was no correlation between the number of monocytes and ASO levels (p=0.637, r=0.087) or between the number of lymphocytes and ASO levels (p=0.412, r=-0.150). There was no correlation between the number of monocytes and lymphocytes and ASO levels in pediatric tonsillitis patients. More research is needed on inflammatory markers such as HsCRP, Procalcitonin (PCT) and Interleukin 6 (IL-6).
Evaluasi Jumlah Leukosit, Jumlah Netrofil, dan Kadar Anti Streptolysin O pada Pasien Tonsilitis Anak Citra Nadia Salsabila; Dita Pratiwi Kusuma Wardani; Arif Mulyanto; Defi Nurul Hayati
Jurnal Biodiversitas dan Bioteknologi Vol 2 No 2 (2025): Biodiverse: Jurnal Biodiversitas dan Bioteknologi
Publisher : Jurnal Biodiversitas dan Bioteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tonsillitis is a common global disease that most frequently affects children, particularly those aged 5–9 years. The primary cause of tonsillitis is infection of the tonsils by Streptococcus ?-hemolyticus Group A bacteria. Leukocytes play a key role in fighting infections, while neutrophils, as the main cells involved in acute inflammation, destroy bacteria through phagocytosis. This study aimed to determine the correlation between leukocyte and neutrophil counts and Anti-Streptolysin O (ASO) levels in children with tonsillitis. This research employed a cross-sectional design involving 32 pediatric tonsillitis patients, both inpatients and outpatients, at Dr. R. Goeteng Taroenadibrata Regional Hospital, Purbalingga. ASO levels were measured using the latex agglutination method, while hematological parameters—total leukocyte and neutrophil counts—were measured using a hematology analyzer based on the flow cytometry method. Data on the correlation between leukocyte and neutrophil counts and ASO levels were analyzed using Spearman’s rho correlation test. The results showed no significant correlation between leukocyte count and ASO level (p = 0.424, r = 0.146), and no correlation between neutrophil count and ASO level (p = 0.653, r = 0.083). There was no significant correlation between leukocyte and neutrophil counts and ASO levels in children with tonsillitis. Further studies are recommended using ASO measurement methods such as ELISA or turbidimetry, as well as additional inflammatory markers including Interleukin-1 (IL-1), Interferon-? (IFN-?), and Tumor Necrosis Factor-? (TNF-?).