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All Journal Indian Journal of Forensic Medicine & Toxicology Asian Journal of Health Research
Widjiati
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Calorie Restriction and Moderate-Intensity Continuous Exercise Decrease Free Fatty Acid Levels and Visceral Fat Weight on High Calorie Diet Female Mice Ahmad Rukhani Lutfi; Lilik Herawati; Widjiati; Gadis Meinar Sari
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14944

Abstract

Objective: This study aimed to analyze the effect of a combination of calorie restriction and moderateintensity continuous exercise on FFA levels and visceral fat weight in female mice exposed to a high-caloriediet.Material and Method: This research was randomized posttest used only control group design with femalemice as the sampl Balb/c mice (Mus musculus), 8 weeks old, 20-30 grams. It was randomly divided into fourgroups, namely C (control group), G1 (moderate-intensity continuous exercise group), G2 (calorie restrictiongroup), and G3 (combination group calorie restriction and moderate-intensity continuous exercise) wherein every group consisted six female mice. Providing a high-calorie diet using ad libitum plus D40 as muchas 3-5% of calories (0.0325 mL/gBB) for 4 weeks. The provision of a high-calorie diet was done usingthe sonde technique. Calorie restriction was done 3x/week in 4 weeks by limiting calorie intake to 50% ofstandard foods. The moderate-intensity continuous exercise was carried out by swimming for 15 minutesplus 6% gBB loading, it was done 3 times/week for 4 weeks. The measurement of cholesterol, visceral fatweight, and FFA level was done at the end of treatments.Results: The results found that the mean of cholesterol of C C (171.00±47.74 mg/dL), G1 (126.00±15.92 mg/dL), G2 (131.50±22.37 mg/dL), G3 (121.17±21.23 mg/dL) dan (p=0.032). The mean weight of visceral fatat C (0.68±0.23 grams), G1 (0.25±0.08 grams), G2 (0.28±0.06 grams), G3 (0.20±0.03 grams) and (p=0.000).The mean FFA levels were at C (1511.34±6.19 µmol/L), G1 (1419.31±53.61 µmol/L), G2 (1458.20±28.89µmol/L), G3 (1451.55±43.77 µmol/L) and (p=0.004).Conclusion: Based on the results of the study, it could be concluded that the provision of calorie restrictionand moderate-intensity continuous exercise significantly reduced cholesterol levels, visceral fat weight, FFAlevels and in female mice exposed to a high-calorie diet
Effect of Cacao on Plasma F2-Isoprostane Level, CD34 and ICAM-1 Expression of Coronary Arteries in Cigarette Smoking Exposed Rats Dina Helianti; Soetjipto; Widjiati; I Ketut Sudiana
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15729

Abstract

This research aimed to evaluate cardioprotective effects of cacao in smoking exposure condition that the effects were mediated through the anti-oxidant pathway by measure plasma F2-isoprostane level, Endothelial Progenitor Cell (EPC) enhancement by expression of CD34, while dysfunction endothelial condition was measured by expression of ICAM-1 in coronary arteries. This study subjected rats, divided into four groups: the normal control group (2 ml of aqua bidest, air exposure); the cigarette control group (2 ml of aqua bidest, cigarette smoke); cacao group 1 (1205 mg/kg BW/day, cigarette smoke); cocoa group 2 (2410 mg/kg BW/day, cigarette smoke). The oxidant biomarker, F2-isoprostane level was assessed using ELISA; CD34, and ICAM-1 expression in coronary arteries by immunohistochemistry. Cacao 1205 mg/kg BW/day significantly decreases plasma F2-isoprostane level, and ICAM-1 expression of coronary arteries in cigarette smoking exposed rat (p < 0.05) but there was not a significant increases CD34 (p < 0.05). Cocoa in cigarette smoke-exposed rats can prevent endothelial dysfunction through decrease F2-isoprostane but not increase CD34. The results of this study can be used as a basis for preventing endothelial dysfunction due to cigarette smoke by using cacao.
The cerebrum and cerebellum of Rattus norvegicus offspring, whose mothers were exposed to a sequence of 14 Mozart compositions during pregnancy, expressed higher levels of BDNF and dendritic density, but not mTORC1 Hermanto TJ; N Cininta M; Widjiati
Asian Journal of Health Research Vol. 4 No. 1 (2025): Volume 4 No 1 (April) 2025
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v4i1.212

Abstract

Introduction: To investigate Brain-Derived Neurotrophic Factor levels, Mammalian Target of Rapamycin Complex 1 levels, and Dendritic Density in the cerebrum and cerebellum of Rattus norvegicus offspring following prenatal Mozart compositions exposure. Material and Methods: An experimental study with a randomized post-test-only control group design was conducted at the Animal Laboratory of the Faculty of Veterinary Medicine, Universitas Airlangga, following ethical clearance. A 13 healthy pregnant female Rattus norvegicus were randomly chosen and exposed to 14 Mozart compositions, beginning on gestation day 10 until delivery, while another 13 served as the control group. At day 20, the rats were sacrificed, 2 offsprings' brains (the heaviest) were extracted, and BDNF levels were analyzed using the ab203573 immunohistochemistry kit, mTORC1 levels using the pSer2448 kit, and Dendritic Density using silver impregnation. Statistical analysis was performed accordingly with a significance level of p < 0.05. Results: BDNF levels and Dendritic density in the cerebrum of the treatment group were higher (2.27 ± 1.41 vs. tr 7.44 ± 2.09, p < 0.05) and (4.42 ± 2.04 vs. 6.67 ± 1.23, p < 0.05). In the cerebellum, BDNF levels and Dendritic density in the treatment group were higher (1 vs. 8, p < 0.05) and (3.34 ± 1.94 and 7.91 ± 2.45, p < 0.05). mTORC1 levels in the treatment group were lower but not statistically significant in both areas. Conclusion: Prenatal 14 Mozart compositions exposure during pregnancy increased Rattus norvegicus offsprings' cerebrum and cerebellum BDNF levels and Dendritic Density. A correlation was found between BDNF levels and dendritic density.
Co-Authors Ahmad Rukhani Lutfi Ayu Putri Yani Binta Dwi Novitasari Budi Santoso Diana Estu Rumahastuti Dina Helianti Dwi Nurdi Puspita Sari Gadis Meinar Sari Hari Basuki Notobroto HERAWATI, LILIK Herlina Puji Angesti Hermanto TJ Hermanto Tri Joewono Heru Santoso Wahito Nugroho Jimmy Yanur Annas Joseph Chandra Relmasira Kusumawardani, Emi N Cininta M Nur Laila Faizah Nurvy Alief Aidillah S.Pd. M Kes I Ketut Sudiana . SOETJIPTO