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PENGARUH EKSTRAK MENGKUDU (Morinda citrifolia) TERHADAP PENGOBATAN PENYAKIT PARASIT ARGULUS PADA IKAN KOMET (Carassius auratus) Era Insivitawati; Tri Ari Setyastuti
COJ (Coastal and Ocean Journal) Vol. 4 No. 2 (2020): COJ (Coastal and Ocean Journal)
Publisher : Pusat Kajian Sumberdaya Pesisir dan Lautan IPB

Disease is a threat that can be obstruct the fish growth. Argulus is one of the parasite that need to be watched out because it can interfere the fish growth, and cause secondary of infections that are dangerous for fish. The purpose of this study was to determine the benefits of noni for survival rate of fish and to determine the appropriate concentration of noni extract for culture the fish. This study uses a descriptive method to explain the results of the study. The results showed that giving noni extract at a dose of 1 drop / liter was able to make the argulus break away from fish and noni extract was effective in increasing fish immunity.

Gambaran Darah dan Histopatologi Insang, Usus Dan Otak Ikan Koi (Cyprinus carpio Koi) yang Diinfeksi Spora Myxobolus koi secara Oral [Haematology and Histopatology of Gills, Intestine And Brain Koi Fish (Cyprinus carpio Koi) Myxobolus koi Orally Infected] Gunanti Mahasri; Kusnoto Kusnoto; Era Insivitawati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i2.11210

Abstract Koi fish (Cyprinus carpio koi) is one of the ornamental fish are preferred. One of the obstalces in the koi fish is a disease caused by infection myxobollus. This research to determine the effect of the infection myxobollus by oral in blood picture and histopathology. Haematology and histopathology is an indicator of the health of the fish to diagnose disease and to determine changes in the fish tissue. The research was conducted in Politeknik Kelautan dan Perikanan, dan Balai Karantina Ikan Kelas I, Juanda Surabaya from October to December 2014. This study uses the 5 treatments and 5 replications with different doses of infection (0,60,80,100 and 120 spora/fish). Data were analyzed with Annova haematology, followed by Duncan 5%. While histopathology were analyzed by semiquantitative scoring mothod used to determine the degree of histology Kluschal Wallis. The result showed that the haematology has significant result, white histopathology signify the presence of significant damage.

Analysis of White Feces Disease (WFD) caused by Vibrio sp. Bacteria and Dinoflagellata in Vannamei Shrimp (Litopenaeus vannamei) in Brackishwater Culture Pond Moh. Awaludin Adam; Irawati Mei Widiastuti; Ernawati Ernawati; Achmad Yani Yayan; Era Insivitawati; Yuliana Yuliana; Rini Fitriasari Pakaya; Agoes Soegianto; Ach. Khumaidi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 1 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i1.26684

Highlight ResearchIndicated that white feces at Vanammei appeared along with the appearance of the disease besides its main trigger that is Vibrio sp.Increase the availability of carbon for photosynthesis process of phytoplankton.Clinical symptom of shrimp attacked by white feces disease is indicated by a change in intestine.AbstractShrimp disease that currently causes economic loss to shrimp farmers is White Feces Disease (WFD). This disease appeared due to several factors, such as poor pond management, unhealthy shrimp seed, and poor water quality which resulted in the appearance of Vibrio sp. bacteria and Dinoflagellate. This study aimed to analyze the cause of WFD outbreak in vannamei shrimp pond. The study method through direct experiment was applied in shrimp pond. Sampling was performed three times in each feeding tray to collect ten shrimps. Overall, sampling was performed twice a week. The Sample Survey Method was used to collect sample in this study. Result of study showed that clinical symptom was observed through changes in pattern and behavior of vannamei shrimp during culture. However, this observation resulted in insignificant data. Vannamei shrimp infected WFD tended to swim slower and often rose to the surface with body color turned red. Moreover, total organic matter (TOM) increased on week-7 along with the increasing growth of plankton, particularly from the Dinoflagellate group. However, bacterial growth of Vibrio sp. on week-7 was insignificant, yet many shrimps were found dead with white feces during that period. The result of analysis indicated that white feces disease was caused by Dinoflagellate besides the main trigger, namely, Vibrio sp.

Exploring the Anti-Menopausal Potential of Rhizophora mucronata Lam. Ethanol Extract: A Comprehensive Study on Estrogen Receptor β Agonist Activity Ernawati, Ernawati; Adam, Moh. Awaludin; Widiastuti, Irawati Mei; Insivitawati, Era; Nikmatullah, Muhammad; Riyadi, Putut Har; Azra, Mohamad Nor
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 3 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.3.414-424

Mangrove is a tropical forest that stores millions of benefits ecologically, biologically, and economically. Rhizophora fruit extract contained bioactive compounds components derived from natural ingredients and scientifically proven to have positive effects on health, among others, to prevent cancer, etc. This study aimed to investigate the potential of R. mucronata ethanol extract as an estrogen receptor β agonist for anti-menopausal purposes. Using a Completely Randomized Design (CRD), 25 mice were divided into five groups: a normal control group (NK), an ovariectomy control group (Ovx), and three ovariectomized groups (Ovx D1, D2, and D3) receiving different doses of the extract (200, 400, and 800 mg.kg-1 BW, respectively). The extract was administered orally, and various measurements were taken, including flavonoid content, using densitometry thin layer chromatography (TLC) and FTIR for functional group characterization. The study found a high rutin content (13.29%) in the fruit. Twelve compounds with potential estrogenic activity were identified, which were analyzed using SwissAdme software. Estradiol levels in serum increased with higher doses of the extract over four weeks. In silico and in vivo analysis showed 5 (five) selected compounds from the ethanol and ethyl acetate fractions with highest (most negative) to lowest binding affinity as candidates for anti-menopausal drugs. The administration of R. mucronata Lam. fruit extract in Ovx D1, Ovx D2 and Ovx D3 gave significantly different effects to each other on rat blood serum estradiol hormone levels. In this study, the dose 400 mg.L-1 BW rat gave P>5 increasing blood serum estradiol levels of ovariectomized rats.

Algae content estimation utilizing optical density and image processing method Kamaluddin, Muhammad Wafiq; Gunawan, Agus Indra; Setiawardhana, Setiawardhana; Dewantara, Bima Sena Bayu; Insivitawati, Era; Asmarany, Anja; Pratama, Ariesa Editya
International Journal of Electrical and Computer Engineering (IJECE) Vol 14, No 6: December 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijece.v14i6.pp6248-6257

One of the factors that influence shrimp cultivation is the presence of algae. Precise knowing algae content in the pond is essential for effective management. Most research in the field of algae species carried out by researchers were observing Chlorella Sp. more than the other algae species, with a particular emphasis on substance concentrations. This study proposed non-invasive techniques for quantifying algae abundance, utilizing optical density (OD) and image processing (IP) methods. Three different algae species are frequently found in Indonesia i.e., Chlorella Sp., Thalassiosira Sp., and Skeletonema Sp. are used as sample. Those samples are cultured and prepared in a certain volume with a certain quantity. For experimental and observation purposes, those samples are then diluted into water based on percentage value. The experimental results provided RGB values, which were then used to establish polynomial equations. To verify these equations, two approaches were employed: synthetic image analysis and evaluation using additional data. The mean average error (MAE) was found to be 3.467 for IP method and 3.513 for OD method. It shows that IP method give better result compared to OD method in this study. However, it is very possible that the two methods will complement each other.

EFEKTIVITAS DAUN KETAPANG (Terminalia catappa) DAN BAWANG PUTIH (Allium sativum) SEBAGAI IMUNOSTIMULAN HERBAL PADA BUDIDAYA IKAN NILA (Oreochromis niloticus) Insivitawati, Era; Wahidi, Budi Rianto; Asmarany, Anja; Hakimah, Nisa; Jayanti, Shara; Setyatuti, Tri Ari; Suseno, Dewi Nurmalita; Sugianti, Budi
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 21, No 2 (2025): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.21.2.138-144

Budidaya ikan nila (Oreochromis niloticus) berpotensi besar, namun rentan terinfeksi bakteri dan memerlukan solusi alternatif pengganti antibiotik sintetis. Daun ketapang (Terminalia catappa) dan bawang putih (Allium sativum) bersifat antimikroba dan imunostimulan, namun efektivitas dan formulasi optimal masih memerlukan penelitian lebih lanjut. Penelitian ini bertujuan mengevaluasi efektivitas kombinasi daun ketapang dan bawang putih untuk mencari alternatif pengendalian bakteri yang lebih aman, mengingat penggunaan obat berlebihan dapat memicu resistensi. Daun ketapang dan bawang putih dipilih sebagai imunostimulan herbal yang potensial menekan bakteri Aeromonas tanpa dampak negatif bagi lingkungan. Metode yang digunakan adalah eksperimen Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan yaitu Perlakuan Kontrol tanpa pemberian ekstrak daun ketapang dan bawang putih, Perlakuan A (komposisi 75 % daun ketapang dan 25% bawang putih), Perlakuan B (komposisi 50 % daun ketapang dan 50% bawang putih), dan Perlakuan C (komposisi 25 % daun ketapang dan 75% bawang putih). Pengujian mencakup uji fitokimia, zona hambat, DPPH (2,2-Diphenyl-1-picrylhydrazyl), analisis kualitas air dan pertumbuhan ikan dengan uji statistik. Hasil menunjukkan kombinasi 50% daun ketapang dan 50% bawang putih (Perlakuan B) merupakan yang terbaik dan berpotensi meningkatkan daya tahan tubuh ikan dengan kadar alkaloid tertinggi sebesar 37,91% (b/b) dengan persentase Relative Percent Difference (RPD) 1,5%, IC50 Uji DPPH 3,79 mg/ml, menghambat pertumbuhan bakteri yang ditunjukkan dengan hasil zona hambat terbesar, pertumbuhan berat terbaik dengan ABW mencapai 17,60 ± 6,84 gram/ekor dan ADG 1,53 g/minggu. Penelitian ini mengindikasikan bahwa kombinasi daun ketapang dan bawang putih dapat menjadi imunostimulan alami yang ramah lingkungan dalam budidaya ikan nila.

PROXIMATE ANALYSIS AND NUTRITIONAL PROFILE OF FISH FEED BASED ON DRIED TUBIFEX WORMS (Tubifex sp.) AND PAPAYA LEAF EXTRACT Wahidi, Budi Rianto; Hafidz, Muhammad Fernanda Pratama; Insivitawati, Era
Jurnal Biosains Pascasarjana Vol. 27 No. 2 (2025): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v27i2.2025.160-165

This study aimed to analyze the nutritional content of fish feed with a novel formulation, combining dried Tubifex worms and papaya leaf extract, to enhance growth and production efficiency in freshwater fish. Proximate tests revealed significant differences between fresh and papaya leaf extract-enriched dried Tubifex worms. Although the protein content of dried Tubifex worms was lower, the addition of papaya leaf extract potentially improved nutritional efficiency through proteolytic enzymes. Dried Tubifex worms enriched with papaya leaf extract offer advantages in storage, buoyancy, and nutritional efficiency, making them a promising feed alternative in aquaculture. Further research is needed to validate their effectiveness at a culture scale.

Antibacterial Effectiveness of Yellow Turmeric (Curcuma longa) and White Turmeric (Curcuma zedoaria) Extracts against Aeromonas salmonicida: Antibacterial Effectiveness of Yellow Turmeric (Curcuma longa) and White Turmeric (Curcuma zedoaria) Extracts against Aeromonas salmonicida Nisa Hakimah; Budi Rianto Wahidi; Tri Ari Setyastuti; Anja Asmarany; Era Insivitawati; Budi Sugianti; Dewi Nurmalita Suseno; Shara Jayanti
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.87885

Aeromonas salmonicida is a major fish pathogenic bacterium responsible for significant losses in aquaculture. The growing concern over antibiotic resistance has driven interest in plant-based antibacterial alternatives. This study examined the extraction yield, phytochemical characteristics, and in vitro antibacterial activity of ethanolic extracts obtained from the rhizomes of Curcuma longa and Curcuma zedoaria against Aeromonas salmonicida. Extraction was conducted using an ethanol-based maceration technique. Qualitative phytochemical screening was subsequently performed, and antibacterial activity was evaluated through the disc diffusion assay at extract concentrations ranging from 10% to 100%. The results showed that C. longa produced a higher extraction yield (7.18%) than C. zedoaria (3.19%). Both extracts contained flavonoids, total phenolics, alkaloids, and tannins, while saponins were not detected. Antibacterial activity against A. salmonicida increased with extract concentration, reaching maximum inhibition at 100%, with zone diameters of 12.97 ± 0.57 mm (C. longa) and 12.17 ± 0.61 mm (C. zedoaria). Significant differences were observed among concentration treatments. In conclusion, ethanolic extracts of Curcuma longa and Curcuma zedoaria demonstrate promising plant-based antibacterial activity against A. salmonicida in vitro, supporting their potential use in aquaculture health management. Nevertheless, further in vivo studies and toxicity evaluations are required before practical application.

Capacity Enhancement of Catfish Farmers through Herbal Immunostimulant Training as a Preventive Strategy in Gelang Village, Sidoarjo: Penguatan Kapasitas Pembudidaya Ikan Lele melalui Pelatihan Imunostimulan Herbal sebagai Strategi Preventif Penyakit Kuning di Desa Gelang, Sidoarjo Dewi Nurmalita Suseno; Tri Ari Setyastuti; Budi Rianto Wahidi; Nisa Hakimah; Era Insivitawati; Shara Jayanti; Anja Asmarany R; Budi Sugianti
Room of Civil Society Development Vol. 5 No. 1 (2026): Room of Civil Society Development
Publisher : Lembaga Riset dan Inovasi Masyarakat Madani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59110/rcsd.871

Jaundice remains a recurrent problem in small-scale catfish aquaculture and contributes to reduced market value and economic losses for farmers. This community service program aimed to enhance the capacity of catfish farmers in Gelang Village, Tulangan District, Sidoarjo Regency, in producing and utilizing herbal immunostimulants as a preventive strategy. The program involved 10 farmers and was implemented through technical training consisting of theoretical sessions, hands-on practice, and mentoring. The immunostimulant introduced was prepared using a simple decoction method from locally available ingredients including Terminalia catappa, ginger, galangal, turmeric, and Curcuma xanthorrhiza. Quantitative evaluation showed an average knowledge increase of 147 percent from pre-test to post-test, with all participants exceeding the minimum improvement target. Practical skill assessment indicated an average mastery score of 94 percent, and 100 percent of participants were able to independently produce the extract. The readiness to adopt the technology reached an average score of 93 percent. Field observations indicated perceived effectiveness in supporting preventive health management practices. However, no laboratory analysis or biological measurements were conducted. This program primarily strengthened farmers’ knowledge, technical skills, and adoption readiness toward locally based preventive approaches that may support sustainable small-scale catfish farming.

Capacity Enhancement of Catfish Farmers through Herbal Immunostimulant Training as a Preventive Strategy in Gelang Village, Sidoarjo: Penguatan Kapasitas Pembudidaya Ikan Lele melalui Pelatihan Imunostimulan Herbal sebagai Strategi Preventif Penyakit Kuning di Desa Gelang, Sidoarjo Dewi Nurmalita Suseno; Tri Ari Setyastuti; Budi Rianto Wahidi; Nisa Hakimah; Era Insivitawati; Shara Jayanti; Anja Asmarany R; Budi Sugianti
Room of Civil Society Development Vol. 5 No. 1 (2026): Room of Civil Society Development
Publisher : Lembaga Riset dan Inovasi Masyarakat Madani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59110/rcsd.871

Jaundice remains a recurrent problem in small-scale catfish aquaculture and contributes to reduced market value and economic losses for farmers. This community service program aimed to enhance the capacity of catfish farmers in Gelang Village, Tulangan District, Sidoarjo Regency, in producing and utilizing herbal immunostimulants as a preventive strategy. The program involved 10 farmers and was implemented through technical training consisting of theoretical sessions, hands-on practice, and mentoring. The immunostimulant introduced was prepared using a simple decoction method from locally available ingredients including Terminalia catappa, ginger, galangal, turmeric, and Curcuma xanthorrhiza. Quantitative evaluation showed an average knowledge increase of 147 percent from pre-test to post-test, with all participants exceeding the minimum improvement target. Practical skill assessment indicated an average mastery score of 94 percent, and 100 percent of participants were able to independently produce the extract. The readiness to adopt the technology reached an average score of 93 percent. Field observations indicated perceived effectiveness in supporting preventive health management practices. However, no laboratory analysis or biological measurements were conducted. This program primarily strengthened farmers’ knowledge, technical skills, and adoption readiness toward locally based preventive approaches that may support sustainable small-scale catfish farming.

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