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All Journal International Journal of Renewable Energy Development BERKALA FISIKA Jurnal Ilmiah Teknik Elektro Komputer dan Informatika (JITEKI) JURNAL ILMIAH PENDIDIKAN FISIKA AL BIRUNI Indonesian Journal of Physics and Nuclear Applications Jurnal Otomasi Kontrol dan Instrumentasi Jurnal Ilmu Fisika Molekul: Jurnal Ilmiah Kimia ASEAN Journal on Science and Technology for Development Jurnal Fisika Indonesia Molekul: Jurnal Ilmiah Kimia International Journal of Renewable Energy Development
Bilalodin Bilalodin
Department Of Physics, Faculty Of Mathematics And Natural Science, Universitas Jenderal Soedirman, Jalan Dr. Suparno Karangwangkal, Purwokerto 53123
Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Physics

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Partial Purification and Characterization of Urease from Red Lentils (Vicia lens (L.) Coss. & Germ.) Zusfahair, Zusfahair; Ningsih, Dian Riana; Bilalodin, Bilalodin; Fatoni, Amin; Setiawan, Ely; Sulistyowati, Aris
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.12920

Abstract

ABSTRACT. Urease is an enzyme that catalyzes the hydrolysis of urea into ammonia and carbon dioxide. A significant application of urease is found primarily in food, medical equipment and biosensor industries. This research aims to analyze the amino acid content of red lentil seeds and the extraction, purification, and characterization of urease from red lentils. The study started by analyzing the amino acid content in red lentil seeds using High-Performance Liquid Chromatography (HPLC). The red lentil seeds were extracted using phosphate buffer pH 7.0 and separated using centrifugal separation technique until crude extract of urease was produced. The crude extract of urease was then concentrated using acetone at varied saturation level (33, 41, 50, 60, and 67%). The fraction with the highest specific activity was then analyzed using SDS-PAGE method and characterized for its pH, incubation temperature, and substrate concentration against the urease activity. The urease activity was determined using Nessler method. The research results showed that red lentils seeds contained all essential amino acids. The highest specific activity was found in the fraction at 50% acetone saturation level (F50) and purity level 6.3 times than the crude extract. The characterization result indicated that F50 was purer than the crude extract. The optimum urease activity of crude extract and F50 was obtained at pH 7.0 and an incubation temperature of 35 °C. The KM value of F50 was lower than crude extract. F50 has a higher affinity for binding to substrates so that the enzyme has higher efficiency in forming the products. Urease from red lentil seeds concentrated using acetone was 50% more potent as a catalyst than the crude extract. The research data will be the basis for the application of this urease. Keywords: Acetone, characterization, partial purification, red lentil, urease
The Synthesized-Hydroxyapatite Powder from Anadara Granosa Shells using Deposition Time Method for Biomedical Applications Sunardi, Sunardi; A’yun, Nidha Aulia Qurrata; Dari, Qorinah Wulan; Aminuddin, Jamrud; Bilalodin, Bilalodin; Praktino, Budi; Yulianti, Evi; Utomo, Agung Bambang Setio; Sari, Kartika
Jurnal Ilmu Fisika Vol 16 No 1 (2024): March 2024
Publisher : Jurusan Fisika FMIPA Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jif.16.1.88-96.2024

Abstract

Hydroxyapatite (HAp) powder, one of the biomaterials derived from natural sources, could be used in biomedical applications. In this research, the synthesized-HAp powder from Anadara Granosa shells as raw materials had a high calcium carbonate content with variations in deposition time using the precipitation method. Variations of deposition time used were 0 (S0), 24 (S24), and 48 (S48) hours. Fourier Transform Infrared (FTIR), X-Ray Diffractions (XRD), and Scanning Electron Microscopy (SEM) were used to investigate the chemical structure, phase analysis, and morphology of the synthesized HAp powder. FTIR results of the S0, S24, and S48 showed that the functional groups ,  and were formed at variations in the deposition time. The XRD results showed that the smallest of crystallite size of S48 was 26.03 nm, and the crystallinity degree of S24 was 38.74%. The grain dispersity of the synthesized-hydroxyapatite powder from SEM results were uniform, agglomeration, and spherical, irregular shape. The Ca, P, Mg, and Si compositions were shown in the synthesized-hydroxyapatite powder. The deposition time affects the synthesized-Hydroxyapatite (HAp) powder from the Anadara Granosa shell, and it is a potential raw material for biomedical applications.
Modeling and Analysis of Percentage Depth Dose (PDD) and Dose Profile of X-Ray Beam Produced by Linac Device with Voltage Variation Bilalodin Bilalodin; Farzand Abdullatif
Jurnal Ilmiah Teknik Elektro Komputer dan Informatika Vol. 8 No. 2 (2022): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/jiteki.v8i2.23622

Abstract

The Percentage Depth Dose (PDD) and dose profile of X-Ray output from a LINAC therapy device have been modeled and analyzed. The research was conducted by simulation method through the use of Particle and Heavy Ion Transport code System (PHITS) program. The LINAC therapy device modeled in this work refers to the Siemens Primus LINAC therapy device, which is operated at 6 MV, 10 MV and 18 MV voltages. Determination of PDD was carried at a depth of 0-30 cm and dose profile at a depth of 0-20 cm in a water phantom, placed at 100 cm from the source, which is exposed to a radiation field area of 10×10 cm2. Results from the modeling of the LINAC therapy device agrees with the actual X-ray apparatus and has produced Bremsstrahlung X-ray. It was found from the analysis of the PDD curve that the maximum doses are at the depth of 1.5 cm, 2.5 cm and 3.4 cm. The value of build up factor for each LINAC voltage agrees with the reference. Additionally,  the results of the analysis of the doses profile suggest that the X-ray output has good degree of uniformity. The flatness of dose profile occurs at the depth of 20 cm with percentage value of flatness at 1.6 %, 1.9 % and 1.2 %. The flatness values are all less than 2%. The flatness values shows ≤ 2 % deviation from reference value, which is below the tolerance range required in a measurement.
Co-Authors A Arifin Abdullah Nur Aziz Abdullah Nur Aziz Agung Bambang Setio Utomo AMIN FATONI Aminuddin, Jamrud Aminuddin, Jamrud Apriliadina, Inessa Putri Arief Hermanto Arief Hermanto Arief Hermanto Arifin, A Aris Haryadi Aris Haryadi, Aris Aryanti, Eva A’yun, Nidha Aulia Qurrata Dari, Qorinah Wulan Dian Riana Ningsih Dwi Satya Palupi Evi Yulianti Farzand Abdullatif Gede Bayu Suparta Jamrud Aminuddin Jayawarsa, A.A. Ketut Kartika Sari Kasesaz, Yaser Kusminarto Kusminarto Kusminarto Kusminarto Luthfia, Adilla Muhtar Effendy Mukhtar Effendi Mukhtar Effendi Mukhtar Effendi Muslihah, Niken Istikhari Nuraini, Aprilia Nafi Nurhayati Nurhayati Nurhayati Nurhayati Pakhrur Razi Praktino, Budi PUJI LESTARI Purwati Purwati R Farzand Abdullatif R. A. Saputro Sehah Sehah Setiawan, Ely Sri Yuniarti Sugito Sugito - Sulistyowati, Aris Sunardi - Sunardi Sunardi Sunardi Sunardi Sunardi Sunardi W Widanarto Widiyani, Agustina Wihantoro Wihantoro Wihantoro Wihantoro Yaser Kasesaz Yohannes Sardjono Yohannes Sardjono Yohannes Sardjono Yohannes Sardjono Yohannes Sardjono Yohannes Sardjono Zarah Irayani Zaroh Irayani Zusfahair Zusfahair