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Partial Purification and Characterization of Urease from Red Lentils (Vicia lens (L.) Coss. & Germ.) Zusfahair, Zusfahair; Ningsih, Dian Riana; Bilalodin, Bilalodin; Fatoni, Amin; Setiawan, Ely; Sulistyowati, Aris
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.12920

Abstract

ABSTRACT. Urease is an enzyme that catalyzes the hydrolysis of urea into ammonia and carbon dioxide. A significant application of urease is found primarily in food, medical equipment and biosensor industries. This research aims to analyze the amino acid content of red lentil seeds and the extraction, purification, and characterization of urease from red lentils. The study started by analyzing the amino acid content in red lentil seeds using High-Performance Liquid Chromatography (HPLC). The red lentil seeds were extracted using phosphate buffer pH 7.0 and separated using centrifugal separation technique until crude extract of urease was produced. The crude extract of urease was then concentrated using acetone at varied saturation level (33, 41, 50, 60, and 67%). The fraction with the highest specific activity was then analyzed using SDS-PAGE method and characterized for its pH, incubation temperature, and substrate concentration against the urease activity. The urease activity was determined using Nessler method. The research results showed that red lentils seeds contained all essential amino acids. The highest specific activity was found in the fraction at 50% acetone saturation level (F50) and purity level 6.3 times than the crude extract. The characterization result indicated that F50 was purer than the crude extract. The optimum urease activity of crude extract and F50 was obtained at pH 7.0 and an incubation temperature of 35 °C. The KM value of F50 was lower than crude extract. F50 has a higher affinity for binding to substrates so that the enzyme has higher efficiency in forming the products. Urease from red lentil seeds concentrated using acetone was 50% more potent as a catalyst than the crude extract. The research data will be the basis for the application of this urease. Keywords: Acetone, characterization, partial purification, red lentil, urease
The Synthesized-Hydroxyapatite Powder from Anadara Granosa Shells using Deposition Time Method for Biomedical Applications Sunardi, Sunardi; A’yun, Nidha Aulia Qurrata; Dari, Qorinah Wulan; Aminuddin, Jamrud; Bilalodin, Bilalodin; Praktino, Budi; Yulianti, Evi; Utomo, Agung Bambang Setio; Sari, Kartika
Jurnal Ilmu Fisika Vol 16 No 1 (2024): March 2024
Publisher : Jurusan Fisika FMIPA Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jif.16.1.88-96.2024

Abstract

Hydroxyapatite (HAp) powder, one of the biomaterials derived from natural sources, could be used in biomedical applications. In this research, the synthesized-HAp powder from Anadara Granosa shells as raw materials had a high calcium carbonate content with variations in deposition time using the precipitation method. Variations of deposition time used were 0 (S0), 24 (S24), and 48 (S48) hours. Fourier Transform Infrared (FTIR), X-Ray Diffractions (XRD), and Scanning Electron Microscopy (SEM) were used to investigate the chemical structure, phase analysis, and morphology of the synthesized HAp powder. FTIR results of the S0, S24, and S48 showed that the functional groups ,  and were formed at variations in the deposition time. The XRD results showed that the smallest of crystallite size of S48 was 26.03 nm, and the crystallinity degree of S24 was 38.74%. The grain dispersity of the synthesized-hydroxyapatite powder from SEM results were uniform, agglomeration, and spherical, irregular shape. The Ca, P, Mg, and Si compositions were shown in the synthesized-hydroxyapatite powder. The deposition time affects the synthesized-Hydroxyapatite (HAp) powder from the Anadara Granosa shell, and it is a potential raw material for biomedical applications.
Pengaruh Lapangan Asimetri Terhadap Kurva Percentage Depth Dose dan Profil Dosis Pada Pesawat LINAC Salsabila, Faizah N.; Bilalodin, Bilalodin; Mario, Aloysius
Prosiding SNFA (Seminar Nasional Fisika dan Aplikasinya) 2025: Prosiding SNFA (Seminar Nasional Fisika dan Aplikasinya) 2025
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/prosidingsnfa.v0i0.115733

Abstract

Karakteristik berkas radiasi pada pesawat LINAC (Linear Accelerator) ditunjukkan oleh kurva Percentage Depth Dose (PDD) dan kurva profil dosis. Kedua kurva ini merepresentasikan perilaku penyampaian dosis secara vertikal (kedalaman) dan lateral (penyebaran melintang) dalam medium. Karakteristik kurva PDD dan profil dosis diduga dipengaruhi oleh bentuk dan ukuran lapangan penyinaran radiasi, khususnya ketika mengunakan lapangan asimetri. Penelitian ini bertujuan untuk mengetahui pengaruh lapangan asimetri terhadap karakteristik kurva PDD dan profil dosis pada pesawat LINAC. Penelitian dilaksanakan di Departemen Radioterapi Rumah Sakit MRCCC Siloam Jakarta menggunakan pesawat LINAC berenergi foton 10 MV. Lapangan simetri berukuran 10×10 cm² digunakan sebagai lapangan standar pengukuran, dan dibandingkan dengan variasi lapangan asimetri berukuran 5×10 cm². Data diperoleh melalui pengukuran menggunakan water phantom dan detektor ionisasi chamber untuk memperoleh data karakteristik sebaran dosis pada berbagai kedalaman. Hasil pengukuran menunjukkan bahwa penggunaan lapangan asimetri berdampak pada penurunan dosis pada kurva PDD dibandingkan dengan lapangan simetri. Selain itu, profil dosis pada lapangan asimetri menunjukkan penurunan homogenitas berkas radiasi yang ditandai dengan nilai flatness melebihi 3%, melampaui batas toleransi standar internasional. Dapat disimpulkan bahwa penggunaan lapangan asimetri memengaruhi karakteristik sebaran dosis pada pesawat LINAC, baik secara kedalaman maupun lateral. Oleh karena itu, pemilihan dan desain lapangan radiasi perlu dipertimbangkan secara cermat dalam perencanaan terapi untuk menjamin penyampaian dosis yang optimal dan aman.)