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Acute Toxicity Test of Jalembi Probiotic Beverages on Mice ddY Anindita, Reza; Sari, Afrinia Eka
BERKALA SAINSTEK Vol 11 No 4 (2023)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v11i4.34514

Abstract

An acute toxicity test is one of the pre-clinical tests needed to determine the safety of the consumption of a product before clinical trials are carried out on humans. The purpose of this study was to confirm whether or not there was a toxic effect of jalembi juice probiotic drink (red guava, lemon, melon, beetroot) on male and female mice observed for 14 days. This research design is experimental. The samples in this study were jalembi probiotic juice drinks with Lactobacillus plantarum starter and 40 male and female ddY mice. This research procedure was carried out by giving jalembi juice as a test preparation with doses of 124, 640, 3200, and 16000 mg/kg BW as much as 0.59 ml in males and females mice. Data analysis was carried out with One-Way ANOVA. This study showed that the administration of probiotic drink jalembi juice with doses of 124, 640, 3200, and 16000 mg/KgBW as much as 0.59 ml did not cause death, changes in body weight, and clinical symptoms in all test animals. It can be concluded that the probiotic drink jalembi juice is categorized as practically non-toxic (> 15 g/KgBW) or passes pre-clinical testing as a safe drink for consumption.
HUBUNGAN TINGKAT STRES DAN FREKUENSI KONSUMSI FAST FOOD DENGAN KEJADIAN DISMENOREA PADA REMAJA DI KABUPATEN BEKASI Iranti, Tasya Putri; Sari, Afrinia Eka
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 18 No 1 (2023): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v18i1.1526

Abstract

Remaja merupakan masa peralihan dari masa anak-anak menuju dewasa, yang dimana masa tersebut akan mengalami perubahan terutama secara biologis. Perubahan yang terjadi secara biologis pada perempuan biasanya ditandai dengan terjadinya menstruasi. Pada perempuan yang sudah mengalami menstruasi, mempunyai peluang terjadinya dysmenorrhea. Dysmenorrhea merupakan keluhan nyeri pada saat haid yang dirasakan pada bagian bawah perut hingga ke punggung bagian bawah. Dysmenorrhea cenderung terjadi lebih sering dan lebih hebat, apabila disertai dengan kondisi stres dan memiliki kebiasaan mengkonsumsi fast food. Penelitian ini bertujuan untuk mengetahui hubungan antara tingkat stress dan frekuensi konsumsi fast food terhadap kejadian dysmenorrhea.
Promosi Gizi Dan Kesehatan Lansia di Panti Sentra Terpadu Pangudi Luhur Bekasi Arindah Nur Sartika; Afrinia Eka Sari; Tri Marta Fadhilah; Guntari Prasetya; Noerfitri Noerfitri; Putri Rahmah Alamsyah; Rohayati Rohayati; Anung Ahadi Pradana; Joni Siahaan
I-Com: Indonesian Community Journal Vol 4 No 3 (2024): I-Com: Indonesian Community Journal (September 2024)
Publisher : Fakultas Sains Dan Teknologi, Universitas Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33379/icom.v4i3.4870

Abstract

Promosi Kesehatan, termasuk promosi gizi di dalamnya, merupakan salah satu upaya untuk mengoptimalisasi kesehatan lanjut usia (lansia). Tim pengabdi STIKes Mitra Keluarga melakukan kegiatan promosi gizi dan kesehatan di Panti Sentra Terpadu Pangudi Luhur Bekasi. Sebanyak 6 orang pengurus mengikuti penyuluhan gizi, 8 orang pengurus mengikuti penyuluhan aktivitas fisik lansia, dan 7 orang lansia mengikuti sharing session dan penilaian status gizi. Terdapat peningkatan skor secara signifikan pada pengetahuan gizi (p= 0.0195, uji Wilcoxon Signed Rank) sehingga dapat dikatakan kegiatan pengetahuan berhasil meningkatkan pengetahuan terkait gizi. Namun, tidak terdapat peningkatan pengetahuan aktivitas fisik secara signifikan (p= 0.3275, uji Wilcoxon Signed Rank). Status gizi lansia sebagai berikut: 72% normal, 14% berat badan kurang, dan 14% berat badan lebih.. Melalui kegiatan ini diharapkan dapat membantu upaya menjaga status gizi pada lansia. Namun, upaya tersebut akan terasa lebih maksimal jika kegiatan pendampingan serupa dapat dilakukan secara berkelanjutan.
Snacking behavior among school-aged children in urban area Sartika, Arindah Nur; Ekasari, Afrinia; Prasetya, Guntari
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 13 ISSUE 3, 2025
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/ijnd.2025.13(2).179-187

Abstract

ABSTRAK Latar Belakang: Mengkonsumsi jajanan dalam sehari dapat memberikan kontribusi masukan energi selain mengonsumsi makanan utama. Namun perilaku jajanan pada anak sekolah di Indonesia menunjukkan beberapa kebiasaan yang tidak sehat seperti seringnya mengonsumsi jajanan berkalori tinggi dan minuman manis. Anak-anak di kota besar mempunyai risiko lebih tinggi mengalami obesitas karena tingginya konsumsi jajanan tidak sehat. Tujuan: Penelitian ini bertujuan untuk melihat perilaku jajanan anak usia sekolah yang tinggal di perkotaan. Metode: Jenis penelitian observasional dengan desain cross sectional study. Sampel penelitian merupakan anak usia sekolah dasar, berasal dari seluruh kelurahan di wilayah Bekasi sebagai salah satu kota megapolitan di Indonesia, meliputi kelurahan: Duren Jaya, Bekasi Jaya, Aren Jaya, dan Margahayu. Sebanyak 213 siswa mengikuti penelitian ini. Siswa diminta mengumpulkan data tentang karakteristik responden dan perilaku jajanan (menggunakan angket terstruktur dan angket frekuensi makanan). Hasil: Hasil penelitian menunjukkan 50,2% anak sering makan jajanan; 79,8% membeli makanan ringan dari kombinasi kantin sekolah, pedagang kaki lima, dan warung makan tetap; 70,4% membeli makanan ringan pada jam istirahat, setelah, dan sebelum sekolah; dan 75,5% menggunakan bahan kemasan plastik. Tidak terdapat hubungan antara hubungan jenis kelamin, kebiasaan sarapan pagi, uang jajan, pendidikan ibu, pendidikan ayah dengan frekuensi jajan (chi-square test menunjukan p-value: 0.175, 0.302, 0.269, 0.104, 0.247). Kesimpulan: Penelitian ini menunjukkan bahwa separuh anak usia sekolah dasar di Kota Bekasi sering mengonsumsi makanan jajanan, hanya 30% siswa yang tidak membeli makanan ringan di sekolah, dan 75% siswa terbiasa jajan makanan dengan kemasan plastik. Hampir 80% siswa terbiasa jajan di dua atau tiga tempat penyedia jajanan, seperti kantin sekolah, pedagang kaki lima, dan warung makan. Frekuensi jajan siswa tidak berhubungan dengan faktor yang berkaitan dengan karakteristik siswa dan pendidikan orang tua. KATA KUNCI: anak usia sekolah; area perkotaan;faktor penentu; perilaku jajan   ABSTRACT Background: During the day, snacks may contribute to energy input besides meals. However, snacking behavior among school children in Indonesia shows some unhealthy habits, such as frequent consumption of high-calorie snacks and sugary beverages. Children in big cities have a higher risk of obesity since they consume unhealthy snacks. Objectives: This study aims to see the snacking behavior of school-age children living in urban populations in Indonesia.Methods: The study is observational with a cross-sectional study design. Samples are school-age children taken from all urban villages in the Bekasi Area, a megapolitan city in Indonesia: Duren Jaya, Bekasi Jaya, Aren Jaya, and Margahayu. A total of 213 students joined the study. Students were asked to complete data about respondent characteristics and snacking behavior (using a structured questionnaire and food frequency questionnaire).Results: The results show that 50.2% of children often eat snacks; 79.8% buy snacks from a combination of school canteen, street vendors, and permanent food stalls; 70.4% buy snacks during break time, after, and before school; and 75.5% use plastic packaging material. There is no association of sex, breakfast habits, pocket money, mother education, and father education with snacking frequency (p-value: 0.175, 0.302, 0.269, 0.104, 0.247).Conclusions: This study shows that half of elementary school children in Bekasi City often consume snacks, only 30% of students do not buy snacks at school, and 75% of students are used to buying snacks in plastic packaging. Almost 80% of students were buying snacks at two or three food vendors, such as school canteens, street vendors, and food stalls. The frequency of snack consumption was not related to factors attributable to students’ characteristics and parents’ education. KEYWORDS: determinant factors; school-age children; snacking behavior; urban areaReceived: 14 Jan 2024; Revised: 05 Dec 2024; Accepted: 14 Feb 2025; Available online: 30 May 2025; Published: 31 Mar 2025.
Uji Nanoemulsi Ecoenzym Terhadap Bakteri Staphylococcus aureus anindita, Reza; Nathalia, Dede Dwi; Beandrade, Maya Uzia; Putri, Intan Kurnia; Sari, Afrinia Eka; Setyodewi, Elisabeth
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.553

Abstract

The discovery of eco enzyme as a fermented liquid from organic vegetable and fruit waste has been used as an organic plant fertilizer, disinfectant, hand sanitizer, antiseptic soap, and domestic waste degradation agent. However, trials of eco enzymes as raw materials for health preparations have not yet been carried out. Seeing this, trial research is needed on the use of ecoenzyme nanoemulsion against Staphylococcus aureus bacteria. This research aims to determine the antibacterial ability of ecoenzyme nanoemulsion against S. aureus bacteria. The design of this research is experimental. The samples used were ecoenzyme nanoemulsions with Formula 1 (F1) 20%, Formula 2 (F2) 25%, and Formula 3 (F3) 30%. This research includes making ecoenzyme, making ecoenzyme nanoemulsion, testing ecoenzyme nanoparticles, and testing ecoenezyme nanoemulsion against S. aureus bacteria using the Kirby-Baeur method. The results showed that the sizes of ecoenzyme nanoparticles in F1 20%, F2 25%, and F3 30% were 38.9 nm, 65.07 nm, and 146.2 nm, respectively. The results of the antibacterial ability test of nanoemulsion eco enzyme F1 20%, F2 25%, and F3 30% against S. aureus produced an inhibitory zone 4.3 mm (resistant), 4.3 mm (resistant), and 6.7 mm (resistant). This research concludes that the eco enzyme nanoemulsion is not effective in inhibiting the growth of S. aureus bacteria.
Snacking behavior among school-aged children in urban area Sartika, Arindah Nur; Ekasari, Afrinia; Prasetya, Guntari
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 13 ISSUE 3, 2025
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/ijnd.2025.13(2).179-187

Abstract

ABSTRAK Latar Belakang: Mengkonsumsi jajanan dalam sehari dapat memberikan kontribusi masukan energi selain mengonsumsi makanan utama. Namun perilaku jajanan pada anak sekolah di Indonesia menunjukkan beberapa kebiasaan yang tidak sehat seperti seringnya mengonsumsi jajanan berkalori tinggi dan minuman manis. Anak-anak di kota besar mempunyai risiko lebih tinggi mengalami obesitas karena tingginya konsumsi jajanan tidak sehat. Tujuan: Penelitian ini bertujuan untuk melihat perilaku jajanan anak usia sekolah yang tinggal di perkotaan. Metode: Jenis penelitian observasional dengan desain cross sectional study. Sampel penelitian merupakan anak usia sekolah dasar, berasal dari seluruh kelurahan di wilayah Bekasi sebagai salah satu kota megapolitan di Indonesia, meliputi kelurahan: Duren Jaya, Bekasi Jaya, Aren Jaya, dan Margahayu. Sebanyak 213 siswa mengikuti penelitian ini. Siswa diminta mengumpulkan data tentang karakteristik responden dan perilaku jajanan (menggunakan angket terstruktur dan angket frekuensi makanan). Hasil: Hasil penelitian menunjukkan 50,2% anak sering makan jajanan; 79,8% membeli makanan ringan dari kombinasi kantin sekolah, pedagang kaki lima, dan warung makan tetap; 70,4% membeli makanan ringan pada jam istirahat, setelah, dan sebelum sekolah; dan 75,5% menggunakan bahan kemasan plastik. Tidak terdapat hubungan antara hubungan jenis kelamin, kebiasaan sarapan pagi, uang jajan, pendidikan ibu, pendidikan ayah dengan frekuensi jajan (chi-square test menunjukan p-value: 0.175, 0.302, 0.269, 0.104, 0.247). Kesimpulan: Penelitian ini menunjukkan bahwa separuh anak usia sekolah dasar di Kota Bekasi sering mengonsumsi makanan jajanan, hanya 30% siswa yang tidak membeli makanan ringan di sekolah, dan 75% siswa terbiasa jajan makanan dengan kemasan plastik. Hampir 80% siswa terbiasa jajan di dua atau tiga tempat penyedia jajanan, seperti kantin sekolah, pedagang kaki lima, dan warung makan. Frekuensi jajan siswa tidak berhubungan dengan faktor yang berkaitan dengan karakteristik siswa dan pendidikan orang tua. KATA KUNCI: anak usia sekolah; area perkotaan;faktor penentu; perilaku jajan   ABSTRACT Background: During the day, snacks may contribute to energy input besides meals. However, snacking behavior among school children in Indonesia shows some unhealthy habits, such as frequent consumption of high-calorie snacks and sugary beverages. Children in big cities have a higher risk of obesity since they consume unhealthy snacks. Objectives: This study aims to see the snacking behavior of school-age children living in urban populations in Indonesia.Methods: The study is observational with a cross-sectional study design. Samples are school-age children taken from all urban villages in the Bekasi Area, a megapolitan city in Indonesia: Duren Jaya, Bekasi Jaya, Aren Jaya, and Margahayu. A total of 213 students joined the study. Students were asked to complete data about respondent characteristics and snacking behavior (using a structured questionnaire and food frequency questionnaire).Results: The results show that 50.2% of children often eat snacks; 79.8% buy snacks from a combination of school canteen, street vendors, and permanent food stalls; 70.4% buy snacks during break time, after, and before school; and 75.5% use plastic packaging material. There is no association of sex, breakfast habits, pocket money, mother education, and father education with snacking frequency (p-value: 0.175, 0.302, 0.269, 0.104, 0.247).Conclusions: This study shows that half of elementary school children in Bekasi City often consume snacks, only 30% of students do not buy snacks at school, and 75% of students are used to buying snacks in plastic packaging. Almost 80% of students were buying snacks at two or three food vendors, such as school canteens, street vendors, and food stalls. The frequency of snack consumption was not related to factors attributable to students’ characteristics and parents’ education. KEYWORDS: determinant factors; school-age children; snacking behavior; urban areaReceived: 14 Jan 2024; Revised: 05 Dec 2024; Accepted: 14 Feb 2025; Available online: 30 May 2025; Published: 31 Mar 2025.
Penguatan Peran Siswa Dalam Rangka Meningkatkan Konsumsi Makanan Bersih, Sehat, dan Aman di SMA N 6 Bekasi Afrinia Ekasari; Arindah Nur Sartika; Maulin Inggraini; Azkiyah Amanda Putri; Audrey Farhanisa Fasya
Jurnal SOLMA Vol. 15 No. 1 (2026)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v15i1.21057

Abstract

Background:  Siswa SMA Negeri 6 Bekasi terbiasa mengonsumsi makanan yang dijual di kantin sekolah. Namun, mayoritas siswa belum pernah mendapat edukasi mengenai  pemilihan makanan bersih, sehat, dan aman. Selain itu, di sekolah tersebut juga belum terdapat peer influencer  di bidang gizi. Tujuan: Kegiatan pengabdian ini memiliki tujuan membentuk perwakilan siswa sebagai peer influencer dan meningkatkan pengetahuan  mereka mengenai makanan yang bersih, sehat, dan aman. Metode: Kegiatan pengabdian dilakukan dengan pembentukan “Generasi BSA” sejumlah 32 siswa. Siswa tersebut mendapat edukasi dengan metode ceramah dengan media berupa modul dan slide powerpoint, serta poster yang ditempel di lingkungan sekolah. Peningkatan pengetahuan diukur melalui skor pre-post test dan tingkat signifikasi diukur dengan Wilcoxon Signed Rank. Hasil: Kegiatan pengabdian ini berhasil membentuk Generasi Bersih, Sehat, dan Aman (BSA) yang merupakan perwakilan siswa kelas XI dan XII. Terjadi peningkatan pengetahuan siswa mengenai makanan bersih, sehat, dan aman pada saat pre-test dan post-test (p-value 0.000). Nilai median pre-test sebesar 60 dan post-test  sebesar 95. Kesimpulan: Kegiatan pengabdian ini membentuk 32 siswa sebagai Generasi BSA. Dari kegiatan edukasi diketahui terjadi peningkatan pengetahuan siswa sebelum dan sesudah pengetahuan mengenai makanan bersih, sehat, dan aman secara signifikan (p-value 0.000).
Nutrition education to increase awareness about stunting among soon-to-be married couples in selected religion affair offices, Bekasi, West Java Sartika, Arindah Nur; Fadhilah, Tri Marta; Sari, Afrinia Eka; Prasetya, Guntari; Noerfitri, Noerfitri
Journal of Community Empowerment for Health Vol 9, No 1 (2026)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.86475

Abstract

Introduction: Nutrition education related to stunting is one of the strategies to tackle stunting incidence. A soon-to-be married couple is the right target to receive information about stunting, since they will be parents, especially a mother in the future. As it is known, the Religious Affairs Office has a pre-marriage training program, but not include nutritional sciences.Methods: A nutrition intervention consisting of nutrition education for men and women before marriage, to attend a mini lecture. The nutrition education program, including subjects related to marriage, is a pre-marriage education program from the selected religious affairs office in Bekasi. It aimed to increase nutrition knowledge, focusing on stunting prevention through family action. Participants were asked to fulfill nutrient adequacy for mothers as early as possible, before pregnancy, during pregnancy, and the lactating phase, as well as nutrient adequacy for infants during the first thousand days of life. They were also asked to ensure their health by joining the government’s program to visit the primary health service for antenatal care during pregnancy, and the integrated health center for growth monitoring after the baby is born. About 71 participants were recruited to do the pre-test, join the nutrition class, and finish the post-test.Results: Showed a double frequency of participants answering one hundred percent correctly during post-test, also most of the participants had an increased score in the post-test, p=0.000 (Wilcoxon signed-rank test). It showed that this program significantly increased participants’ knowledge about stunting.Conclusion: Nutrition education through simple lectures effectively improved knowledge about stunting. It is recommended to incorporate stunting topics into the pre-marriage education program as a preventive measure to reduce stunting. 
The Potential of Tepache Drinks with Addition of Guava Skin as Probiotic Drink Afrinia Eka Sari; maulin Inggraini; Arindah Nur Sartika
Journal of Global Nutrition Vol 6 No 1 (2026)
Publisher : Ikatan Sarjana Gizi Indonesia (ISAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53823/jgn.v6i1.195

Abstract

The development of probiotic beverage innovation is now growing. Probiotic products are in demand by the public because of their benefits in maintaining digestive track Health. One drink that has potential to be a probiotic drink is tepache, a fermented drink made from pineapple skin dissolved in a sugar solution. The fermentation process that occurs produces lactic acid bacteria that have potential to be probiotics. In this study, an experimental design was used where tepache drinks were made with six different formulas, where the differences are found in the types of sugar, namely palm sugar and granulated sugar, and the amount of sugar added: F1 (16%), F2 (22%) anf F3 (27%) is the same for added brown sugar in F4, F5, F6. Identification of bacteria was carried out by growing on PCA (Plate Count Agar) media to determine the Total Colony Count and MRSA (de Mann Rogosa Agar) media to grow lactic acid bacteria in this case selectively in the grow of lactobacillus. From the results of observations for 3 days, showed that bacteria grew on PCA media at a dilution 10-4, 10-5, and 10-6 and on MRSA media grew at a dilution of 10-4. From the pH measurements, the results obtained for all formulas were pH 4 and brix degress 60. Organoleptic test showed that this tepache drink was liked by panelists, data taken from 20 panelist because its refreshing, sour taste and fruity aroma.The study concluded that tepache has the potential to be a probiotics beverage, as the bacteria found to grow were lactic acid bacteria with pH that matches the probiotic drink and was preffered by panelists
Analysis of Escherichia coli and Salmonella sp. Contamination in Snacks and Food Handlers of Elementary School Children in West Bekasi Afrinia Ekasari; Arindah Nur Sartika; Maulin Inggraini; Reza Anindita
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.373-377

Abstract

Food safety is a preventive measure against the risk of disease caused by microorganisms, chemicals, and physical materials that can contaminate food. Salmonella sp. and E. coli is a common food poisoning agent found in school snacks. Elementary school students are a vulnerable group to microbial contamination due to their frequent snacking habits. Therefore, this study aims to analyze E. coli and Salmonella sp. contamination in school snacks in the West Bekasi school area, Bekasi City. The research design of this study was a laboratory-based experiment. Data collection method: Data was collected from three different locations: SDN Bintara 1, SDN Jakasampurna 1 and SDN Kotabaru 3. Snack food testing was carried out using TPC testing, to determine the presence of E. coli contamination using EMBA media and Salmonella sp. using SSA media. The result of TPC is above 71,4% of school children’s snacks are classified as unsafe only 4 types of food are classified as safe, namely onigiri, papeda, cilor, and meatballs on skewers. There are 2 foods that grow in EMBA media, namely udang rambutan and cibay, it is possible that both foods are contaminated with E. coli.