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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Entomologi Indonesia Nandur
WAYAN ADIARTAYASA
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science

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PELATIHAN PENGENDALIAN PENYAKIT BUSUK BERAIR PADA BUAH SALAK DI DESA DUDA TIMUR, KECAMATAN SELAT KABUPATEN KARANGASEM W. Adiartayasa; I.N. Wijaya; I.G.N. Bagus; I.M.M. Adnyana; I.K. Siadi
Buletin Udayana Mengabdi Vol 17 No 3 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.807 KB) | DOI: 10.24843/BUM.2018.v17.i03.p03

Abstract

Salak (Salacca edulis Reinw.) merupakan tanaman asli Indonesia dan buah salak mempunyai nilai yang cukup tinggi dan disukai oleh konsumen Indonesia. Salak Bali mempunyai pasar yang cukup baik di kota besar di Jawa seperti Surabaya, Solo, Semarang dan Jakarta. Desa Sibetan dan Duda Timur merupakan penghasil buah salak yang terbanyak. Umur simpan buah salak Bali berkisar antara 6-7 hari pada suhu penyimpanan 29oC, selama penyimpan bobot buah salak mengalami penurunan 20 persen. Kekerasan daging buah salak cenderung mengalami penurunan ditandai dengan makin melunaknya daging buah. Kerusakan buah salak dapat terjadi sejak berada pada pertanaman, waktu panen, pasca panen sampai ke pemasaran berkisar 40%. Kerusakan buah salak dapat terjadi akibat luka, memar, pencoklatan, buah pecah kulit, dan penyakit busuk berair. Menurut Adiartayasa (2004) kerusakan buah salak disebabkan oleh jamur yaitu Ceratocystis sp. dan Botryodiplodia sp. yang menyebabkan kulit buah salak menjadi berwarna coklat kehitaman dan berair, kulit buah mudah pecah dan terkelupas, serta daging buah busuk dan berair serta berwarna coklat. Lebih lanjut ekstrak daun sirih yang diperoleh dari solven metanol 5-15% memiliki daya hambatan yang tinggi terhadap pertumbuhan Botrydiplodia sp. pada media PDA. Sedang ekstrak daun sirih mempunyai kemampuan yang lebih rendah dalam menghambat pertumbuhan Botrydiplodia sp. Pengendalian penyakit Busuk Berair pada buah salak dilaksanakan pada Kelompok Tani dan Ternak Tabu Nandini di Desa Duda Timur, Kecamatan Selat, Kabupaten Karangasem pada hari Senen, 14 Agustus 2017. Kegiatan pelatihan dilaksanakan pada Wantilan Kelompok Tani dan Ternak Tabu Nandini dan pemaparan pelatihan dibantu dengan alat bantu LCD serta dihadiri oleh 35 orang petani dan 6 orang dosen. Peserta mampu mengidentifikasi dan menunjukkan gejala penyakit Busuk Berair pada buah salak. Lebih dari 100 persen peserta dapat menunjukkan gejala penyakit Busuk Berair pada buah salak. Pengendalian penyakit Busuk Berair pada buah salak dalam penyimpanan dan pemasaran dapat menggunakan ekstrak daun sirih.
HAMA DAN PENYAKIT PADA TANAMAN JERUK SERTA PENGENDALIANNYA I N. Wijaya; W. Adiartayasa; I G.P. Wirawan; M. Sritamin; M. Puspawati; I M. Sudarma
Buletin Udayana Mengabdi Vol 16 No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Sebagian besar penduduk Desa Katung, Kecamatan Kintamani, Kabupaten Bangli sebagai petani jeruk yang tergabung dalam kelompok Subak Abian Kerta Winangun. Pertanaman jeruk di daerah ini sangat berhasil, sehingga pendapatannya meningkat. Namun belakangan, petani resah karena tanaman jeruknya terserang hama dan penyakit terutama penyakit CVPD (Citrus Vein Phloem Degeneration) dan hama lalat buah. Oleh karena itu perlu diadakan penyuluhan dan pelatihan tentang pengelolaan hama dan penyakit pada tanaman jeruk. Adapun tujuan penyuluhan dan pelatihan ini untuk memberikan pengetahuan dan keterampilan kepada para petani setempat tentang bahaya serangan hama dan penyakit serta pengendaliannya pada tanaman jeruk meliputi : gejala serangan, faktor-faktor yang mempengaruhi perkembangan hama dan penyakit, cara penularan penyakit serta cara pengendaliannya. Metode yang digunakan dalam pelatihan ini adalah penyampaian materi di ruangan dan praktek di perkebunan jeruk. Adapun tujuan penyuluhan dan pelatihan ini untuk memberikan pengetahuan dan keterampilan kepada para petani setempat tentang bahaya serangan hama dan penyakit serta pengendaliannya pada tanaman jeruk meliputi : gejala serangan, faktor-faktor yang mempengaruhi perkembangan hama dan penyakit, cara penularan penyakit serta cara pengendaliannya. Pelatihan ini dipandu oleh 5 (lima) orang pelatih yang berasal dari Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana. Pelatihan diikuti diikuti oleh 50 orang peserta. Penceramah memaparkan materinya dengan menggunakan alat peraga berupa power point dan menggunakan spesimen berupa gejala serangan hama dan penyakit. Kemudian dilanjutkan dengan praktek lapangan selama 60 menit. Masing-masing peserta diberi tugas untuk mencari tanaman jeruk yang terserang hama dan penyakit serta penyebabnya. Dalam pelaksanaan pelatihan tersebut, petani sangat antusias untuk mengikuti penyuluhan. Hal ini terbukti dari banyaknya pertanyaan-pertanyaan yang diajukan. Untuk mengetahui keberhasilan kegiatan ini, maka dilaksanakan evaluasi dengan pengisian kuisioner sebelum dan sesudah pelatihan.
AWAS BAHAYA PENYAKIT CVPD DAN TEKNIK PENGENDALIANNYA PADA TANAMAN JERUK I. N. Wijaya; M Sritamin; W Adiartayasa; I. G. N Bagus; M Sudarma; N. M. Puspawati
Buletin Udayana Mengabdi Vol 13 No 2 (2014): Vol 13, No. 2 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Education and training on integrated pest management of CVPD (Citrus Vein Phloem Degeneration) was held at Sengkaduan,Taro village, Gianyar regency on 14 August 2014. The activities aimed to improve the farmers’ knowledge of CVPD and integrated pest management. The training was attended by 44 participants from local farmer groups of Sengkaduan village. The method used were lectures, demonstration and practice in the citrus field. All participants participated enthusiastically and hope they have the next intensive training of the citrus culture.Keywords : Training, CVPD, Sengkaduan
PELATIHAN BUDIDAYA TANAMAN JERUK BEBAS PENYAKIT CVPD DI DESA KERTA GIANYAR W. Adiartayasa; I.N. Wijaya; I. G.P. Wirawan; N.N. Darmiati; I K. Siadi
Buletin Udayana Mengabdi Vol 16 No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Penyakit CVPD (citrus vein phloem degeneration) merupakan penyakit penting pada tanaman jeruk. Penyakit menyebar melalui bibit tanaman jeruk yang telah terinfeksi CVPD dan ditularkan melalui serangga vektor D. citri. Tanaman jeruk yang terserang CVPD memperlihatkan gejala daun menguning atau klorosis, warna tulang daun tetap hijau, ukuran daun menjadi kecil dan daun menjadi tebal dan kaku. Hasil pengamatan tanaman secara morfologi di Kecamatan Kintamani didapatkan 10 jenis tanaman jeruk yaitu jeruk Siam, Selayar, Besakih, Tejakula, Manis, Nipis, Purut, Lemo, dan jeruk Bali. Gejala penyakit CVPD pada masing-masing jenis tanaman jeruk mempunyai gejala klorosis bervariasi. Pelatihan ini bertujuan untuk menambah pengetahuan budidaya tanaman jeruk bebas penyakit CVPD. Pelatihan ini telah dilaksanakan di Kantor Desa Kerta, pada hari Rabu tanggal 10 Agustus 2016. Kegiatan ini diikuti oleh 20 orang petani dan lima orang dosen Fakultas Pertanian Unud. Hasil evaluasi yang didapatkan 15 orang menguasi 90 persen materi, dan 5 orang menguasai 60 persen materi. Petani mengharapkan adanya pelatihan dan pendampingan yang berkelanjutan mengenai budidaya tanaman jeruk.
Identifikasi Mikoriza Vesikular Arbuskular pada Rhizosfer Tanaman Lamtoro (Leucaena leucocephala) dan Kaliandra (Calliandra calothyrsus) serta Perbanyakannya dengan Media Zeolit NI WAYAN PUSPARINI DHARMAPUTRI; I NYOMAN WIJAYA; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Vesicular Arbuscular Mycorrhizae Identification of Lamtoro (Leucaena leucocephala) and Kaliandra (Calliandra calothyrsus) Rhizosphere and Its Spore Multiplication in Zeolit MediaSongan village was located in the district of Kintamani, Bangli. The plants population were dominated by lamtoro and kaliandra. This plant was included in the Fabaceae (Leguminoseae) family which capable of forming nodules and symbiotic association to mycorrhizae. This study aimed to determine the types mycorrhizal of rhizosphere lamtoro and kaliandra plants and determine the effectiveness of zeolite media and corn symbiont plant in propagation of VAM. The experiment was conducted from Desember 2014 to Februari 2015, in the Laboratory of Genetic Resources Unit and Molecular Biology University of Udayana. Spore isolation was done by conducting wet sieving method. Roots colonization percentages were calculated by root staining method and spores multiplication through trapping culture method. The results showed that VAM spores found in the rhizosphere lamtoro plants are three genera that resembled Acaulospora, Glomus, and Scutellospora whereas in the rhizosphere of kaliandra plants found two genera that resembled Acaulospora and Glomus. Mycorrhizal structures found in the rhizosphere of lamtoro and kaliandra plants were vesicles and inner spores.VAM from the rhizosphere of lamtoro and kaliandra plants can be reproduced using zeolite media and corn symbionts.
Deteksi Penyakit Citrus Vein Phloem Degeneration (CVPD) Dengan Teknik Polymerase Chain Reaction (PCR) pada Daun Tanaman Jeruk Yang Memiliki Pola Gejala Klorosis Berbeda RIDA MELANI; WAYAN ADIARTAYASA; I NYOMAN WIJAYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.2, April 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Citrus Vein Phloem Degeneration (CVPD) Disease Detection With Polymerase Chain Reaction (PCR) Technique In Citrus Plant Leaves That Have Different Chlorosis Symptom Patterns Detection of Citrus Vein Phloem Degeneration (CVPD) disease with Polymerase Chain Reaction (PCR) on citrus leaves that have different pattern of chlorosis symptoms was conducted at laboratory of Genetic Resources and Biology Molecular, Udayana University. This study aims to determine the presence of Liberibacter asiaticus in citrus leaves that have different patterns of chlorosis symptoms using a specific primer 16S rDNA (OI1 and OI2c). DNA amplified on 1% agarose gel obtained DNA bands of 1160 bp on four samples with a pattern of symptoms of chlorosis.
Organogenesis Tanaman Bawang Merah (Allium ascalonicum L.) Menggunakan Umbi Secara In Vitro pada Media Dasar Murashige and Skoog yang Diperkaya Vitamin B5 dengan Naftalene Acetic Acid dan 6-Benzyl Amino Purine KATARINA IKA NOVIANITA; MADE SRITAMIN; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.3, Juli 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Organogenesis of Shallot Plant (Allium ascalonicum L.) Using Shallot Bulb with In Vitro Method on Murashige and Skoog Basic Media that Enriched by Vitamin B5 with Naftalene Acetic Acid and 6-Benzyl Amino Purine This study was conducted to determine the effect of the combination of NAA (Naftalene Acetic Acid) and BAP (6-Benzyl Amino Purine) in MS media which can stimulate organogenesis in the onion (Allium ascalonicum L.) Biru Lancor variety in vitro. This experiment used four treatments, namely P1 (NAA 1 mg / L + BAP 2 mg / L), P2 (NAA 2 mg / L + BAP 2 mg / L), P3 (Vitamin B5 + NAA 1 mg / L + BAP 1) mg / L), and P4 (Vitamin B5 + NAA 2 mg / L + BAP 2 mg / L). Based on observations made on the growth and development of onion bulbs in vitro, treatment P3 with the combination of concentration 1 mg / L NAA and 1 mg/L BAP with the addition of vitamin B5 can stimulate organogenesis well to the number of leaf shoots, leaf length, number of roots and root length. This media provides the best results for the observed parameters compared to other treatments.
Induksi Mutasi Kromosom dengan Kolkisin pada Tanaman Anggur Merah (Vitis vinifera L. Varietas Prabu Bestari) Melalui Pembentukan Kalus Secara In Vitro DIMINDA ELA SRI ERTINA BR GINTING; MADE SRITAMIN; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Induction of Chromosome Mutations with Colchicin in Red Grape (Vitis vinifera L. Prabu Bestari Varieties) Through In Vitro Callus Formation This study aims to determine the colchicine concentration that can induce chromosome mutations in red grape, carried out in vitro. This study used a completely randomized design (CRD) with 9 treatments and 3 replications so that the number of experimental units was 27 bottles of culture. The sterilized red grape node explants were immersed in colchicine at the concentration according to the treatment for 24 hours. The colchicine concentration used was 0.015% (K1), 0.020% (K2), 0.025% (K3), 0.030% (K4), 0.035% (K5), 0.040% (K6), 0.045% (K7), 0.050% ( K8) and 0% (K0) as controls. The effect of immersion in several colchicine concentrations on chromosome mutations in explants of red grape nodes that form callus cannot be observed because the chromosomes are not clearly visible, and the number of chromosomes cannot be calculated. Callus was formed in colchicine immersion treatment with a concentration of 0.015% (K1) and 0.035% (K5). Callus on K5 was formed faster, namely 10 weeks after planting, while K1 was at 11 weeks after planting. The average callus diameter on K5 was larger with a size of 4.3 mm, while on K1 it was 2 mm.
Deteksi Penyakit Citrus Vein Phloem Degeneration (CVPD) dengan Teknik Polymerase Chain Reaction (PCR) pada Tanaman Jeruk di Bali NI PUTU SWARI MEITAYANI; WAYAN ADIARTAYASA; I NYOMAN WIJAYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.2, April 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACTS Detection of Citrus Vein Phloem Degeneratin (CVPD) Disease by Polymerase Chain Reaction (PCR) Technique on Citrus Plant in Bali CVPD disease is one of many important disease on citrus plant caused by Liberobacter asiaticum. The mechanism of infection is not yet known, so that the efforts to control the disease have not been adequately. In the research, we tried to detect CVPD disease on citrus plant at several locations in the centre of the citrus plantation in Bali used the PCR technique. The results of reseach showed eleven from eighteen sample is positive have contain for the L. asiaticum that show size of DNA 1160 bp, because the size of 1160 bp DNA bands is expression by L. asiaticum, then the citrus leaf samples were detected positive for the L. asiaticum. Based on the results of the calculation incidence of the disease with percentage of attack is 54,38% and the intensity of CVPD diseased plants was 9,86% showed CVPD disease was spread over eight villages consist of Katung, Belancan, Bayung Gede, Awan, Catur, Pengotan, Pelaga and Petang. Keyword: Citrus Vein Phloem Degeneration (CVPD), Liberobacter asiaticum, Polymerase Chain Reaction (PCR)
Isolasi dan Identifikasi Bakteri Selulolitik pada Sampah Organik di TPA Suwung Denpasar FADEL ALKAHFI; WAYAN ADIARTAYASA; I GEDE PUTU WIRAWAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.2, April 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Isolation and Identification of Cellulolytic Bacteria in Organic Waste at TPA Suwung Denpasar Organic waste contains a lot of lignocellulosic material which can be degraded by cellulases. The degradation of cellulose requires three types of enzymes produced by microorganisms. Cellulolytic bacteria are one of the microorganisms involved in the decomposition process and produce cellulase enzymes which can degrade organic matter. This study aims to isolate and test the ability of cellulolytic bacteria in the organic waste of TPA Suwung Denpasar by isolating morphological identification, and quantitative cellulase test. The results of this study found twenty-seven (27) bacterial isolates that were able to grow on CMC media and were classified based on colony morphology and obtained 3 different isolate groups. The isolates that had been grouped were coded A, B, and C. The colony of the isolated isolates appeared to be round and wrinkled. Some surfaces are convex, raised, and hilly and are clear, yellows in color, and white. Isolates A and C were gram positive, while isolate B was gram negative. Based on the calculation results of the Cellulolytic Index value, it was found that isolate B had the highest IS and was in the high category.
Co-Authors Abd. Rasyid Syamsuri DIMINDA ELA SRI ERTINA BR GINTING Evy Prima FADEL ALKAHFI GUSTI AYU KOMANG CANDRA PARWATI GUSTI PUTU DINTYA PUTRA HERLINDAH CHOIRI I G. N. Bagus I G. Ngurah Bagus I GEDE BAGUS DWIPANANDA I Gede Ketut Susrama I GEDE PUTU WIRAWAN I Gusti Ngurah Bagus I K. Simpati I KADEK PURNAWIRAWAN PUTRA I Ketut Siadi I Ketut Suada I KETUT SUMIARTHA I KOMANG JULIARTA I M. Sudarma I Made Mega Adnyana I N. Wijaya I N. WIJAYA I NYOMAN WIJAYA I Putu Anom I WAYAN SUAMBA I. G. N Bagus I. N. Wijaya I.G.N. Bagus I.M.M. Adnyana I.N. Wijaya IKA NURHAYATI KATARINA IKA NOVIANITA KETUT AYU YULIADHI KETUT SIMPATI Ketut Srie Marhaeni Julyasih Khamdan Khalimi M Sudarma M. Puspawati MADE SRITAMIN MADE SUDANA MAHARDHIKA DWI PUTRA WIJAYA Merlyna Rosalia NI MADE ADHYA NIDIDHYA SANI NI NENGAH DARMIATI NI PUTU SWARI MEITAYANI NI WAYAN PUSPARINI DHARMAPUTRI RIDA MELANI Romulia Trielva Apriliani Sasadara, Maria Malida Vernandes WHISNU IDA BAGUS Wijaya I N. Yundari, Yundari