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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Entomologi Indonesia Nandur
WAYAN ADIARTAYASA
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science

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Deteksi Keberadaan Penyakit CVPD (Citrus Vein Phloem Degeneration) dengan Teknik PCR (Polymerase Chain Reaction) di Dusun Untalan Desa Jungutan Kecamatan Bebandem Kabupaten Karangasem I KADEK PURNAWIRAWAN PUTRA; WAYAN ADIARTAYASA; I MADE MEGA ADNYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.4, Oktober 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Detection of CVPD (Citrus Vein Phloem Degeneration) Disease Using PCR Technique of Citrus from Untalan Sub Village, Jungutan Village, Bebandem District, Karangasem Regency This research aims to know about presentation of CVPD disease on citrus plants and detect CVPD disease using PCR technique with specific primer called 16S rDNA. This research was conducted at Laboratory of Genetics Resource and Biology Moleculer, Udayana University. Result for visual monitoring of presentation in CVPD symptom in four locations is 25%, 37%, 27%, 19%, and the average is 27%. The result for average the presentation of CVPD symptom in the shoot of citrus in each locations is 7.9%, 15.73%, 8.9%, and 7.07%. The isolation of leaves citrus DNA were appeared on 1% agarose in electrophoresis showed DNA band. The result of DNA amplified that were appeared on 1% agarose in electrophoresis showed DNA bands 1160 bp. 3 samples from 4 with specific symptom of CVPD gave positive PCR reaction. DNA Bands 1160 bp is expression by Liberobacter asiaticum, than the sample from Untalan Sub Village were detected positive for the Liberobacter asiaticum and the samples were infected by CVPD disease.
Aplikasi Teknik Polymerase Chain Reaction (PCR) Terhadap Variasi Gejala Penyakit Citrus Vein Phloem Degeneration (CVPD) pada Beberapa Jenis Daun Tanaman Jeruk GUSTI PUTU DINTYA PUTRA; WAYAN ADIARTAYASA; MADE SRITAMIN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.2, April 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Application Polymerase Chain Reaction (PCR) Technique to Variation Symptoms Disease Citrus Vein Phloem Degeneration (CVPD) on Several Types of Citrus Leaves CVPD disease is one of the major diseases in citrus plant. CVPD disease spread from plant to plant by insect vectors Diaphorina citri, and from one region to another region infected by seed. CVPD disease causes symptoms, the leaves become chlorotic with veins remain green. This study aims to determine the PCR method can detect CVPD of citrus from mild to severe symptoms. CVPD symptoms observed on several types of citrus leaves (Siam, Selayar, Besakih, Tejakula, Sweet, Purut, Lime, Lemo, and pomelo) showed chlorosis symptoms from mild to severe. Then the plants were symptomatic will be identified molecular in the Biotechnology Lab. After electrophoresis on almost all samples showed DNA bands with size 1160 bp. Therefore 1160 bp was had bacteri Liberobakter, the plants reacted positive with Liberobakter asiaticum Keyword: CVPD, Liberobacter asiaticum, PCR
Isolasi dan Uji Degradasi Bakteri Selulolitik dari Sampah Organik di TPST-3R Kertalangu dan TPST-3R Nangun Resik terhadap Bunga Jepun Bali MAHARDHIKA DWI PUTRA WIJAYA; WAYAN ADIARTAYASA; I NYOMAN WIJAYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.4, Oktober 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Isolation and Degradation Test of Cellulolytic Bacteria from Organic Waste at TPST-3R Kertalangu and TPST-3R Nangun Resik Against Jepun Bali Flowers Cellulolytic bacteria are microorganisms that produce cellulase enzymes that can synergistically hydrolyze crystalline cellulose into smaller oligosaccharides and eventually become glucose which can be used by these microorganisms as a source of nutrients for their growth. Cellulose acts as an inducer in cellulase synthesis which has two functions, namely as an inducer in enzyme synthesis and as a carbon source for cell growth. This study aims to obtain bacteria that are able to degrade organic waste samples using several methods, namely bacterial isolation, selective media, identification of bacterial colony morphology and qualitative bacterial degradation test. The results of this study indicated the presence of bacterial growth in selective CMC media and showed the ability of bacteria to degrade organic waste samples on Gelatine media. The results of bacterial isolation on CMC media with a dilution level of 10-8 were found 14 bacterial isolates in the TPST-3R Kertalangu sample and 16 bacterial isolates in the TPST-3R Nangun Resik sample. Each isolate was classified based on colony morphology, obtained three different isolates. Isolates taken from TPS Kertalangu were coded A, B, and C while isolates taken from TPST-3R Nangun Resik were coded E, F, and G. Most of the surface is convex, and flat. Based on the results of the calculation of the cellulolytic index, it was found that only isolate G was in the medium category and the other isolates had a high category value. The formation of a clear zone around the colonies that grew on CMC media showed that the isolate had cellulolytic activity measured qualitatively. The results of the analysis of the degradation ability of the Bali Jepun flower samples showed that isolate F was the fastest degraded isolate with an average of 3.5 days.
Pengendalian Jamur Akar Putih (Rigidoporus sp.) Penyebab Penyakit Layu pada Tanaman Cengkeh ( Syzygium aromaticum L. ) secara Hayati dan Nabati di Rumah Kaca I KOMANG JULIARTA; MADE SUDANA; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Controlling Root Fungus White (Rigidoporus sp.) Causes wilt disease in plants Clove (Syzygium aromaticum L.) in Biological Greenhouse Control of Root White Fungus (Rigidoporus sp.) Causes wilt disease on clove plants (Syzygium aromaticum L.) by using Biological and Botanical controls in Greenhouse, Aims of this study to determine the effectiveness of plant-based materials and the biological control of wilt disease on cloves plants. The method used in this study was a completely randomized design. This research was conducted in the greenhouse using 10 treatments and 3 replications. The results showed that the treatment of some biological and Botanical controls in the trials showed that the treatment of P. fluorecens have the ability to encourage vegetative growth of cloves plants, and Trichoderma sp. is able to reduce the degree of damage to the plants wilt disease of cloves. Keywords: Cengkeh, the degree of damage, Rigidoporus sp., biological and botanical controls
Isolasi dan Identifikasi Fungi Mikoriza Arbuskular (Fma) secara Mikroskopis pada Rhizosfer Tanaman Jeruk (Citrus sp.) di Desa Kerta, Kecamatan Payangan, Kabupaten Gianyar I WAYAN SUAMBA; I GEDE PUTU WIRAWAN; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Arbuscular Mycorrhizal Fungi (AMF) by Microscopic (AMF) in Rhizosphere of Citrus Plant (Citrus sp.) in Kerta Village, Payangan District, Gianyar Regency Arbuscular Mycorrhizal Fungi (AMF) is a group of organisms from fungi that describes a form of mutualism between symbiotic fungi and plant roots. AMF has great potential as a biological fertilizer because the microorganism which has a very important role in facilitating the absorption of nutrients in the soil to increase plant growth, in addition AMF also function as a biological barrier against pathogen that infect the roots, increasing the availability of water for plants to grow and improve hormone boosters. The purpose of the study was to determine the types of AMF that exist in the rhizosphere of citrus plant and determine the presence of infection by the AMF in the citrus plant roots in the  village of Kerta. The results of the isolation and identification of AMF spores in the rhizosphere of citrus plants in the village of Kerta found 14 different types of AMF spores. This study found 7 type of Glomus, 5 types of Gigaspora and 2 types of Acaulospora. Those types were determined by using the characteristic of their spores. Observations on root finding that the AMF  infection in the form vesicles and arbuscular structures. Keywords : Arbuscular Mycorrhizal Fungi, Glomus, Gigaspora, Acaulospora, Vesicles and Arbuscular.
Analisis Homologi Fragmen DNA CVPDr dari Jeruk Kinkit Trophasia trifolia Menggunakan BLAST Protein Dan BLAST Nukleotida WHISNU IDA BAGUS; I GEDE PUTU WIRAWAN; I WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Homology Analysis Of CVPDr DNA Fragment from 13 Citrus Variety in Bali Using Nucleotide Blast And Protein Blast CVPDr from Trhiphasia trifolia is predicted to have to increase resistance fromCVPD, but the mechanism of this DNA is still largely unknown. The first step of DNAanalyzing is by using homology with Nucleotide and Protein BLAST. BLAST canidentify, similarity search, and predict the function of DNA. The goal of this researchis to compare CVPDr DNA fragment from T. Trifolia with sequences from NCBIGenbank and finding it’s significant homology using nucleotide BLAST and proteinBLAST. Nucleotide BLAST result shows that “DNA Citrus nobilis cultivar Tabanancitrus vein phloem degeneration r protein (CVPDr) gene, partial cds” have the highesthomologs, the other sequences that’s shown by BLAST does not have high enoughsimilarity to be homologus. Protein BLAST show “citrus vein phloem degeneration rprotein [Citrus nobilis]” have the highest homologs, but even if the other BLAST resulthave percentage of similarity lower than 55% there are still possibility of structuralhomolog because the E value is lower than 0.01.
Isolasi dan Identifikasi Bakteri Selulolitik pada Sampah Upacara Agama di Pura Goa Lawah Klungkung NI MADE ADHYA NIDIDHYA SANI; WAYAN ADIARTAYASA; I GEDE PUTU WIRAWAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.2, April 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Isolation and Identification of Cellulolytic Bacteria in Religious Ceremony Waste at Goa Lawah Temple Klungkung Cellulolytic bacteria are bacteria that can hydrolyze cellulose complexes into smaller oligosaccharides and finally glucose. Cellulolytic bacteria synthesize a set of enzymes that can hydrolyze cellulose called cellulase enzymes. This study aims to isolate and identify cellulolytic bacteria that have the potential to degrade cellulose by taking organic waste samples from TPS Pura Goa Lawah Klungkung. Characterization was carried out by growing selected pure isolates on CMC media and then dripping with congored 0.1% to test their cellulolytic potential. The results of bacterial isolation at 10dilution-8 obtained 28 bacterial isolates that were able to grow and utilize cellulose as a carbon source. From the isolation results obtained The 4 bacterial isolates each had different morphological characters capable of producing clear zones with a diameter of 10 mm: 5 mm respectively; 3 mm and 4 mm. The results of the isolation of bacterial colonies appeared to be round and irregular in shape, had clear and cream colors, had a convex and flat surface and all isolates had smooth edges. Type C bacterial isolates included gram-negative groups, bacterial isolates A, B and D included gram-positive groups and all bacterial isolates A, B, C and D were rod-shaped. The degradation ability based on the cellulolytic index value of bacterial isolate A was in the highest category compared to bacterial isolates B, C and D.
Deteksi Keberadaan Penyebab Penyakit Citrus Vein Phloem Degeneration (CVPD) secara Molekuler pada Tanaman Jeruk Siam (Citrus nobilis Lour var. microcarpa Hassk) berdasarkan Variasi Gejala Klorosis IKA NURHAYATI; WAYAN ADIARTAYASA; I GUSTI NGURAH BAGUS
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.4, Oktober 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Detection of the Presence of Citrus Vein Phloem Degeneration (CVPD) Disease Causing in Molecular on Citrus (Citrus nobilis Lour var. microcarpa Hassk)which Based on Variation of Chlorosis Symptoms This research was aimed to identify the presence of Liberobacter asiaticum which caused of Citrus Vein Phloem Degeneration disease on citrus leave with different chlorosis symptoms. This research was conducted at Laboratory of Genetics Resources and Biology Molecular, Udayana University. The leaves sample were measured the chlorophyll content using a chlorophyll meter and then were identified in molecular using Polymerase Chain Reaction (PCR) technique, using primer O11 and O12 which will amplified the specific fragmen of 16S rDNA. The result for chlorophyll content indicated that the leaf samples were containing 26.80, 36.52, 36.62, 40.74, 44.56, 47,06, 58.44 and 65.16 SPAD.DNA amplification showed that the leaves which contained chlorophyll at 58.44 and 65.16 SPAD didn’t show DNA bands with size 1160 bp, and leaves which contained chlorophyll at26.80, 36.52, 36.62, 40.74, 44.56 and 47.06 SPAD showed DNA bands 1160 bp. Therefor the DNA bands 1160 bp is expression by L. asiaticum, then the citrus leaf samples were detected positive for the L. asiaticum and the samples were positive infected by CVPD disease, so that the chlorophyll content of citrus leaves at ?47.04 SPAD can be used as basis to diagnosis CVPD disease on citrus plants.
Kultur Jaringan Tanaman Anthurium (Anthurium andraeanum var. tropical) pada Media MS dengan Penambahan Zat Pengatur Tumbuh BAP dan NAA HERLINDAH CHOIRI; I KETUT SUADA; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.3, Juli 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Tissue Culture of Anthurium Plants (Anthurium andraeanum var. tropical) in MS Media with Growth Regulating Substances BAP and NAA Anthurium (Anthurium andraeanum var. tropical) is an ornamental plant that has been cultivated on a wide scale in the floriculture industry. The advantages of anthurium ornamental plants has beautiful flowers that are suitable for ornamental plants and cut flowers, and the cut flowers have good opportunities in the domestic and international markets. So makes it suitable to be in-vitro developed to produce seedling that are disease resistant, quality and quantity. This experiment was to determine the optimal concentration of 6-Benzylaminopurine and Napthalene Acetic Acid to induce callus from leaves and shoots from anthurium seeds on Murashige and Skoog base media. Experiments on leaf explants using factorial completely randomized design with 25 treatment levels of growth regulator substances BAP and NAA. The results showed that BAP and NAA treatments could increase the growth of anthurium plants. The treatment that has the best result in anthurium leaf curvature is A3B2 (5 mg/l NAA + 3 mg/l BAP), with the fastest curvature time is 2 hst and the explant curves 100%. The treatment that has the best results in shoots induction from anthurium seeds is the treatment of A3B3 (5 mg/l NAA + 5 mg/l BAP) with the appearance of shoots at 7.33 days after planting, having 8.92 of shoots, having 3.33 of leaves, and having 5.85 of roots. The A3B3 treatment showed significantly different from the control treatment. The appearance of shoots on control treatment was 8.66 days after planting, having 3.66 of shoots, having 3.33 of leaves, and having 2.33 of roots. The combination of 5 mg/l BAP + 5 mg/l NAA (A3B3) was the best treatment for shoots growth and was suitable for the purpose of multiplying anthurium seedling.
Uji Efikasi Formulasi Rizobakteri Pantoea agglomerans GTA24 dalam Mengendalikan Penyakit Rebah Semai yang Disebabkan oleh Sclerotium rolfsii pada Tanaman Kedelai GUSTI AYU KOMANG CANDRA PARWATI; KHAMDAN KHALIMI; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Efficacy Test of Pantoea agglomerans GTA24 rhizobacteria Formulations in Controling Damping off  Disease Caused By Sclerotium rolfsii on Soybean Plants The experiment aimed to determine the effectiveness of the P. agglomerans GTA24 rhizobacteria formulations in controlling damping off diseases. The experiment was conducted in vitro and in greenhouse test. The experiment was a randomized complete blocks with five replications. The treatment consists of four types of P. agglomerans GTA24 rhizobacteria formulations is pellets formula, powder formula, compost formula, and the gel formula. The results showed that the application of P. agglomerans GTA24 formulations significantly inhibited the fungal growth. P. agglomerans GTA24 showed strong inhibitory activity agains    Sclerotium rolfsii on PDYA medium percentage of inhibitory activity about 96,30%. The lowest disease incidence was attained by the treatment of gel formula, in which only 15,56% of the soybean plants were infected.   Keywords : rhizobacteria formulations, damping off disease, soybean plants
Co-Authors Abd. Rasyid Syamsuri DIMINDA ELA SRI ERTINA BR GINTING Evy Prima FADEL ALKAHFI GUSTI AYU KOMANG CANDRA PARWATI GUSTI PUTU DINTYA PUTRA HERLINDAH CHOIRI I G. N. Bagus I G. Ngurah Bagus I GEDE BAGUS DWIPANANDA I Gede Ketut Susrama I GEDE PUTU WIRAWAN I Gusti Ngurah Bagus I K. Simpati I KADEK PURNAWIRAWAN PUTRA I Ketut Siadi I Ketut Suada I KETUT SUMIARTHA I KOMANG JULIARTA I M. Sudarma I Made Mega Adnyana I N. Wijaya I N. WIJAYA I NYOMAN WIJAYA I Putu Anom I WAYAN SUAMBA I. G. N Bagus I. N. Wijaya I.G.N. Bagus I.M.M. Adnyana I.N. Wijaya IKA NURHAYATI KATARINA IKA NOVIANITA KETUT AYU YULIADHI KETUT SIMPATI Ketut Srie Marhaeni Julyasih Khamdan Khalimi M Sudarma M. Puspawati MADE SRITAMIN MADE SUDANA MAHARDHIKA DWI PUTRA WIJAYA Merlyna Rosalia NI MADE ADHYA NIDIDHYA SANI NI NENGAH DARMIATI NI PUTU SWARI MEITAYANI NI WAYAN PUSPARINI DHARMAPUTRI RIDA MELANI Romulia Trielva Apriliani Sasadara, Maria Malida Vernandes WHISNU IDA BAGUS Wijaya I N. Yundari, Yundari