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All Journal HAYATI Journal of Biosciences Maspari Journal Maspari Journal Jurnal Ilmu Kelautan Spermonde Prosiding Seminar Biologi BIOMA : Jurnal Biologi Makassar International Journal of Applied Biology Jurnal Ilmu Alam dan Lingkungan Jambura Journal of Health Sciences and Research YARSI Medical Journal Vivabio : Jurnal Pengabdian Multidisiplin
Rosana Agus
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Analisis Residu Amoksisilin Pada Hepar dan Ventrikulus Ayam Petelur di Pasar Tradisional Makassar Raymond Tumanduk; Muhammad Nasrum Massi; Rosana Agus; Firdaus Hamid
Jurnal Ilmu Alam dan Lingkungan Vol. 14 No. 2 (2023): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v14i2.27900

Abstract

Residu antibiotik dalam pangan asal hewan terjadi karena tidak memperhatikan waktu henti obat, melebihi dosis yang dianjurkan dan digunakan sebagai feed additive dalam pakan. Residu antibiotik dapat mengancam kesehatan manusia seperti alergi, keracunan, resistensi bakteri dan gangguan jumlah mikroflora dalam saluran pencernaan. Penelitian ini bertujuan untuk mengetahui adanya residu antibiotik golongan amoksisilin pada ayam petelur di pasar tradisional Kota Makassar. Metode yang digunakan adalah pengambilan 24 sampel hepar dan 24 ventriculus ayam petelur pada 4 pasar tradisional di Kota Makassar. Pengukuran kadar residu antibiotik pada sampel yang positif dengan uji sensitivitas antibiotik dilakukan menggunakan HPLC (High Performance Liquid Chromatography). Hasil pengukuran kadar residu antibiotik amoksisilin pada sampel hepar diperoleh konsentrasi 0.0005-0.0010 mg/kg dan pada sampel ventrikulus konsentrasi 0.0002-0.0020 mg/kg. Kadar residu antibiotik amoksisilin pada sampel hati dan ventriculus dibawah nilai Batas Maksimum Residu (BMR) yang ditetapkan SNI 01-636-2000 yaitu 0.01 mg/kg. Hasil penelitian menunjukkan bahwa residu antibiotik amoksisilin yang terdapat pada hepar dan ventrikulus ayam petelur pada 4 pasar tradisional di Kota Makassar berada dibawah nilai BMR.
Diversifikasi Olahan Rumput Laut Protein Tinggi Dalam Upaya Penurunan Angka Stunting di Kabupaten Pangkep, Sulawesi Selatan: Diversification of Processed High Protein Seaweed in Efforts to Reduce Stunting Rates in Pangkep Regency, South Sulawesi Magdalena Litaay; Syahribulan; Rosana Agus; Kurniati Umrah Nur; Mustika Tuwo; St Fauziah; Early Septiningsih4; St. Nurliah Sanusi; Agung Alfian; Mastan; Fausia; Putri Anriani; Zaraswati Dwyana
Vivabio: Jurnal Pengabdian Multidisiplin Vol. 5 No. 3 (2023): VIVABIO: Jurnal Pengabdian Multidisiplin
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/vivabio.v5i3.51116

Abstract

The coronavirus (COVID-19) pandemic continues to increase, the transmission, spread, and death rates are getting higher so the government is implementing large-scale social restrictions on people's activities in Indonesia. This high transmission can be caused by poor community behavior towards prevention programs that have been informed by the Government, therefore it is necessary to carry out dissemination and counseling related to COVID-19 to increase public knowledge in prevention and transmission to minimize the spread of COVID-19. Since April 2020, COVID-19 has been confirmed to have spread in 34 provinces in Indonesia. So that various regulations were issued to reduce the level of spread of COVID-19 starting from wearing masks, washing hands, keeping a distance, staying away from crowds and reducing mobility, large-scale social restrictions, imposing restrictions on community activities, vaccination programs in doses 1-3, and finally the health protocol for the transition period. Technological developments have encouraged the delivery of information to be more interactive. One of the uses of technology is the delivery of spatial information through ArcGIS Story Maps. ArcGIS Story maps are a way to visualize maps and nonspatial data in advanced web applications. The results of using Story maps are in the form of presenting information and counseling media in the form of interactive maps that contain an overview of COVID-19, case conditions, distribution levels based on urban villages, and how to handle them to related rules through the Story map feature. Keywords:  COVID-19; ArcGIS Online; Story Maps; Corona Virus ABSTRAK Kebutuhan masyarakat Indonesia saat ini akan bahan pangan sudah semakin meingkat seiring dengan dengan meningkatnya jumlah penduduk. Kebutuhan gizi masyarakat harus selalu terpenuhi, tetapi seiring dengan waktu sumber bahan pangan semkin berkurang sehingga perlu untk mengantisipasi kebutuhan gizi terutama protein. Saat ini kebutuhan akan protein dibuat dengan mengolah daging buatan, dari berbagai sumber dan sekarang yang menjadi pusat perhatian adalah rumput laut. Kabupaten Pangkep termasuk daerah  penghasil berbagai jenis rumput laut. Euchema dan Gracillaria adalah jenis rumput laut yang sudah dikenal oleh masyarakat, akan tetapi banyak jenis rumput laut berprotein tinggi yang belum dikenal oleh masyarakat yang dapat diolah menjadi produk makanan bergizi tinggi. Kegiatan pengabdian ini dilaksanakan di Dinas Pengendalian Penduduk Keluarga Berenca Pemberdayaan Perempuan Perlindungan Anak (DP2KBP3A) Kabupaten Pangkep Sulawesi Selatan. Salah satu upaya yang dilakukan adalah melaksanakan pelatihan diversifikasi produk rumput laut  adalah dengan melakukan inovasi produk pangan bergizi. Solusi yang ditawarkan melalui penyuluhan tentang jenis rumput laut yang berprotein tinggi dan pelatihan pembuatan produk bahan pangan berbasis rumput laut, selain menambah penggunaan fungsi rumput laut dan melatih ketrampilan untuk membuat produk inovasi dan dapat dijadikan usaha untuk menambah pendapatan rumah tangga. Keterlibatan mitra dalam kegiatan ini DP2KBP3A Kabupaten Pangkep adalah menyediakan sarana dan prasarana yang dibutuhkan selama kegiatan berlangsung dan mengkordinir kehadiran peserta pelatihan pada saat kegiatan pengabdian. Evaluasi untuk mengetahui penguasaan materi peserta pelatihan mulai dari produksi dan penggunaan produk tersebut di masyarakat diberikan dalam bentuk kuisioner. Luaran dari kegiatan ini berupa publikasi pada jurnal pengabdian pada masyarakat indeks Sinta  dan produk inovasi rumput laut.  Kata Kunci:  Ulva lactuca, Euchema; Gizi; Gracilaria; Pangkep; Stunting    
Cloning and Production of Antigen 85A Mycobacterium tuberculosis for Diagnostic Latent Tuberculosis: a Preliminary Study Agus, Rosana; Sjafaraenan; Samara, Yulianti; Massi, Muhammad Nasrum
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.374-381

Abstract

The main challenge in the management of Tuberculosis (TB) is diagnosing quickly and accurately, especially Latent Tuberculosis Infection (LTBI). LTBI detection was carried out using the Tuberculin Skin Test (TST) and Interferon Gamma Release Assay (IGRA). In TB endemic areas, these two examinations have limitations, so current research is directed at finding specific antigens for the diagnosis of LTBI. One of the potential proteins is Antigen 85A (Ag85A) Mycobacterium tuberculosis (Mtb) encoded by Fibronectin-binding protein A (FbpA). The Ag85 complex induces the proliferation of T-cells and interferon-gamma in most healthy individuals infected with Mycobacterium tuberculosis, Mycobacterium leprae, and BCG-vaccinated mice, making it a potential antigen. This study aims to clone and produce recombinant protein Ag85A from Mtb in Escherichia coli BL21. The methods used were ligation to the pET-32a expression vector, transformation to Escherichia coli BL21, and production of protein by IPTG induction. Characterization of recombinant clones by colony PCR and sequencing. The results obtained were that the fbpA gene isolated from Mtb clinical isolate had been amplified, and the PCR product was 900 bp. The production of Antigen 85A has been successfully carried out and produces 44 kDa.
MOLECULAR DETECTION AND CHARACTERIZATION OF THE  fbpA GENE IN MULTI DRUG RESISTANT (MDR) Mycobacterium tuberculosis ISOLATES FROM MAKASSAR Widyastuti, Helmy; Agus, Rosana; Wahyudi, Dedeng
Jambura Journal of Health Sciences and Research Vol 7, No 4 (2025): OKTOBER: JAMBURA JOURNAL OF HEALTH SCIENCES AND RESEARCH
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/jjhsr.v7i4.33729

Abstract

Antibiotics have been widely used to treat tuberculosis (TB), yet the emergence of drug-resistant strains remains a serious global concern, highlighting the need for new molecular biomarkers to assess treatment success more accurately. Mycobacterium tuberculosis adheres to host tissues through the fbpA gene, which plays a vital role in bacterial virulence and persistence. The novelty of this study lies in exploring the fbpA gene as a potential biomarker for evaluating TB treatment response, differing from previous research that mainly targets drug-resistance genes such as rpoB, katG, or inhA. This study offers a new perspective by focusing on a virulence-related gene that may reflect bacterial survival and therapy outcomes, particularly in Indonesian clinical samples where such data remain scarce. The study aimed to assess the potential of the fbpA gene as a treatment biomarker by detecting a 900 bp fragment using molecular techniques. Ten sputum samples were collected from the Tuberculosis Unit of HUM-RC, Hasanuddin University Hospital, Makassar, and underwent sputum decontamination, Ziehl-Neelsen staining, acid-fast bacilli examination, DNA isolation, PCR amplification, and gel electrophoresis. The results showed the presence of DNA bands in all samples, with variations in thickness and intensity corresponding to DNA concentration. These findings suggest that the fbpA gene could serve as a rapid and reliable molecular biomarker for monitoring bacterial load and evaluating treatment efficacy, supporting the development of more precise diagnostic tools for multidrug-resistant TB management.
Biodiversity of Marine Tunicates at Samalona Waters, Sangkarang Archipelago, Indonesia Litaay, Magdalena; Santosa, Slamet; Johannes, Eva; Agus, Rosana; Moka, Willem; Darmansyah Tanjung, Jennyta Dhewi
Jurnal Ilmu Kelautan SPERMONDE VOLUME 4 NUMBER 1, 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v4i1.3616

Abstract

The study aims to know the biodiversity and community structure of marine tunicate of Samalona waters. The present study is part of biodiversity assessment for marine resources of Sangkarang Archipelago SW Makassar Indonesia. Field campaign was conducted from October to November 2016. Sample collection was done at depth of 3 m and 5 m by using Line Intersection Transect (LIT) method combination plot. Two transects size of 50 m were placed parallel to a shore line at three stations (sta.) at Samalona waters. Plot size 2.5 x 2.5 m was placed side by side of transect and all tunicate inside plot was noted, identified, counted and photographed. Sample collection was collected by using SCUBA and under water camera. Environmental parameters including water temperatue, salinity, dissolved oxygen, clarity, current speed, and wind speed were measured in situ. Data were analysed using ecological indices including species composition and density, Shanon Wienner species diversity, Evenness, and Morisita Indices. The result indicates that there are 18 species of tunicates present at depth of 3 m and 7 meters of Samalona waters, respectively. The result of the ecological analysis shows that species diversity is categorized moderate and no dominant species. Environment parameters indicatess that water quality at Samalona waters is in good condition to support tunicates.
BIODIVERSITY OF MARINE TUNICATES IN SAMALONA WATERS, SANGKARANG ARCHIPELAGO, INDONESIA Litaay, Magdalena; Santosa, Slamet; Johannes, Eva; Agus, Rosana; Moka, Willem; Tanjung, Jennyta Dhewi Darmansyah
Jurnal Ilmu Kelautan SPERMONDE VOLUME 4 NUMBER 1, 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v4i1.3920

Abstract

The study aims to know the biodiversity and community structure of marine tunicate in Samalona waters. The present study is part of biodiversity assessment for marine resources of Sangkarang Archipelago SW Makassar Indonesia. Field campaign was conducted from October to November 2016. Sample collection was done at 3 and 7 m depth by using Line Intersection Transect (LIT) method combined with a quadrat (plot). Two 50 m transects were placed parallel to a shore line at three stations (sta.) at Samalona waters. A quadrat (plot) (2.5 m x 2.5 m) was placed side by side of the line transect and all tunicates in the transect was recorded, identified, counted and photographed. Samples were collected by using SCUBA and under water camera. Environmental parameters including water temperatue, salinity, dissolved oxygen, clarity, current and wind speed, were measured in situ. Data were analysed using ecological indices including species composition and density, Shanon Wienner species diversity, Evenness, and Morisita Indices. The result indicates that there are 18 species of tunicates present at 3 m as well as 7 m depth of Samalona waters.. Result of the ecological analysis shows that species diversity can be categorized as moderate and there were no dominant species. Environmental parameters indicates that water quality at Samalona waters was in good condition to support tunicates.
Detection of the PR5 Gene Associated with Downy Mildew Resistance and  Genetic Diversity Analysis of S2 Lines of Local Maize (Zea mays L.) From South Sulawesi Using SSR Markers Rahma, Sitti; Juhriah; Agus, Rosana
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.475-489

Abstract

A significant decline in corn production is often associated with downy mildew (Peronosclerospora maydis), while maize varieties with genetic resistance to this disease are still relatively limited. A molecular-based approach is needed, such as Phatogenesis Related (PR5) gene detection, to identify maize genotypes that are potentially resistant to downy mildew. This study aims to identify the presence of the PR5 gene and analyze genetic diversity in local maize from South Sulawesi, Srikandi kuning (national variety), and Carotenoid sync 3 from International Maize and Wheat Improvement Center (CIMMYT) to support the acceleration of downy mildew-resistant plant breeding programs. PR5 gene detection was carried out by extracting RNA according to the Total RNA Mini Kit Plant (Geneaid) procedure followed by PCR techniques with specific primers. Genetic diversity analysis was carried out using 15 polymorphic SSR primers. DNA amplification showed that 23 individuals were detected as containing the PR-5 gene from 30 samples tested based on the results of agarose gel electrophoresis. The PIC value obtained from the Simple Sequence Repeats (SSR) primers showed a high level of genetic diversity ranging from 0.64 to 0.93 with an average of 0.85. The genetic similarity matrix was calculated and analyzed using the UPGMA method using NTSYS version 2.2, producing a dendrogram with two main clusters. Cluster I has only one individual with a large genetic distance, while Cluster II is divided into two subclusters, IIA and IIB, reflecting the genetic closeness of most individuals. Individuals with PR5 genes and high genetic diversity were identified as potential candidates for use in a superior maize breeding program resistant to downy mildew disease.
Co-Authors Agung Alfian Andi Arfan Sabran Andi Zulkifli AS Arfan Sabran Asaad Maidin Azis, Moh. Ivan Darmansyah Tanjung, Jennyta Dhewi Early Septiningsih4 Eva Johannes Eva Johannes Fahruddin Fahruddin Fausia Firdaus Hamid Handayani Lolodatu Juhriah Magdalena Litaay Magdalena Litaay Marlina Ummas Genisa Mastan Mochammad Hatta Mochammad Hatta Mohc Hatta Mohc Hatta, Mohc Moka, Willem Muh. Nasrum Massi Muh. Ruslan Umar Muhammad Nasrum Massi Mustika Tuwo Nismawati Nismawati Nur Haedar Nur Haedar Nur, Kurniati Umrah Putri Anriani Raymond Tumanduk Rizalinda Sjahril S.Retnoningrum, Debbie Samara, Yulianti Sitti Rahma, Sitti Sjafaraenan Sjafaraenan, Sjafaraenan Slamet Santosa St Fauziah St. Nurliah Sanusi Syahribulan Tanjung, Jennyta Dhewi Darmansyah Wahyudi, Dedeng Widyastuti, Helmy Zaraswati Dwyana Zaraswati Dwyana