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All Journal ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Proceedings of International Conference on Multidiciplinary Research Cakradonya Dental Journal Odonto dental journal Jurnal of Syiah Kuala Dentistry Society
Ridha Andayani
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences

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Perbandingan Jumlah Koloni Streptococcus Sp, Lactobacillus Sp Dan Candida Sp Di Dalam Rongga Mulut Pasien Skizofrenia Rumah Sakit Jiwa Banda Aceh Ridha Andayani; Abdillah Imron Nasution; Muhammad Qadri
Cakradonya Dental Journal Vol 6, No 1 (2014): Juni 2014
Publisher : FKG Universitas Syiah Kuala

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Abstract

Pasien skizofrenia dapat mengalami masalah gigi dan mulut yang sama dengan orang normal sebagai populasi umum. Namun bukti menunjukkan bahwa mereka memiliki resiko lebih besar mengalami penyakit di rongga mulut dan lebih membutuhkan perawatan rongga mulut. Terjadinya penyakit di rongga mulut sangat erat kaitannya dengan peningkatan jumlah koloni mikroorganisme seperti Streptococcus sp, Lactobacillus sp dan Candida sp. Peningkatan jumlah koloni mikroorganisme dapat meningkatkan status mikroorganisme tersebut menjadi patogen yang menyebabkan suatu penyakit di rongga mulut. Penelitian ini bertujuan untuk mengetahui perbandingan jumlah koloni Streptococcus sp, Lactobacillus sp dan Candida sp di rongga mulut. Penelitian ini adalah penelitian deskriptif dengan pendekatan eksperimental laboratoris yang dilakukan di Rumah Sakit Jiwa Banda Aceh. Subjek penelitian sebanyak 47 pasien skizofrenia laki-laki dan perempuan. Pada subjek dilakukan pengambilan sampel di rongga mulut dengan menggunakan cotton wooden steril yang kemudian dibawa ke laboratorium untuk dikultur dan dihitung jumlah koloni mikroorganismenya. Berdasarkan hasil penelitian menunjukkan jumlah koloni Streptococcus sp yang dikultur pada media selektif TYS20B sebanyak 93,2 x 106 CFU/ml, Lactobacillus sp yang dikultur pada media selektif MRSA sebanyak 0,6 x 106 CFU/ml, dan Candida sp yang dikultur pada media selektif SDA sebanyak 30,5 x 106 CFU/ml. Streptococcus sp merupakan mikroorganisme paling dominan pada rongga mulut pasien skizofrenia Rumah Sakit Jiwa Banda Aceh sehingga dapat dikatakan karies gigi sangat rentan terjadi di rongga mulut pasien tersebut.
UJI AKTIVITAS ANTIFUNGAL EKSTRAK KULIT PISANG BARANGAN (Musa paradisiaca L.) TERHADAP Candida albicans Ridha Andayani; Afrina .; Kartika Sari
Cakradonya Dental Journal Vol 9, No 1 (2017): Juni 2017
Publisher : FKG Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v9i1.9874

Abstract

Candida albicans (C. albicans) adalah jamur oportunistik yang pada keadaan tertentu dapat menjadi patogen di rongga mulut dan menyebakan kandidiasis oral. Penanganan kandidiasis oral umumnya menggunakan obat-obatan antifungal sintetik yang dapat menimbulkan efek samping. Kulit pisang barangan (Musa paradisiaca L.) adalah bagian yang sering dianggap tidak bermanfaat namun mengandung banyak komponen antifungal seperti alkaloid, saponin, steroid, triterpenoid, kuinon, polifenol dan flavonoid. Tujuan penelitian ini adalah untuk mengetahui aktivitas antifungal ekstrak kulit pisang barangan terhadap pertumbuhan C. albicans. Pada penelitian ini, kulit pisang barangan diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Ekstrak kulit pisang barangan yang telah diuji fitokimia, diuji aktivitas antifungalnya menggunakan metode dilusi dengan Standard Plate Count (SPC). Hasil penelitian menunjukkan bahwa ekstrak kulit Musa paradisiaca L. berpengaruh terhadap pertumbuhan C. albicans dengan konsentrasi hambat minimum (KHM) sebesar 12,5% dan Konsentrasi Bunuh Minimum sebesar 100%.Kata kunci : Candida albicans, antifungal, kulit pisang
PENGARUH DURASI PEMAPARAN LARUTAN FLUORIDE DENGAN KONSENTRASI 0,15% TERHADAP PERUBAHAN KEKASARAN PERMUKAAN DENTIN Abdillah Imron Nasution; Ridha Andayani; Putri Disa Maulida
Cakradonya Dental Journal Vol 9, No 2 (2017): Desember 2017
Publisher : FKG Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v9i2.9744

Abstract

Fluoride sering digunakan sebagai bahan aktif maupun bahan desensitisasi di dalam pasta gigi. Konsentrasi standar fluoride yang boleh terkandung dalam pasta gigi di Indonesia tidak boleh lebih dari 0,15% (1500 ppm). Terpaparnya fluoride dengan dentin dapat mendukung remineralisasi dengan stabilisasi hidroksiapatit. Tujuan penelitian ini adalah untuk melihat pengaruh durasi pemaparan fluoride dengan konsentrasi 0,15% terhadap perubahan kekasaran permukaan dentin. Atomic Force Microscopy digunakan untuk mengukur kekasaran permukaan dentin dan pengukuran selanjutnya menggunakan software Gwyddion v.2.30. Dua belas gigi premolar digunakan sebagai spesimen dan dipotong pada area mahkota dekat dengan cementoenamel junction kemudian permukaan dentin dihaluskan menggunakan SiC paper 600-grit. Spesimen dikelompokkan ke dalam 4 kelompok yaitu : Kontrol, Etsa, Fluoride 3 menit dan Fluoride 15 menit. Hasil dari penelitian menunjukkan bahwa nilai rata-rata kekasaran permukaan dentin dari yang tertinggi hingga terendah diawali pada kelompok : etsa (1.850.37 m), k ontrol (1.760.32 m), fluoride 3 menit (1.740.19 m) dan f luoride 15 menit (1.620.37 m). Hasil uji analisis statistik one-way ANOVA dan uji Post Hoc LSD menunjukkan tidak ada perbedaan yang signifikan pada tiap-tiap kelompok (p0,05). Dapat disimpulkan bahwa durasi pemaparan larutan fluoride 0,15% belum mampu mengurangi kekasaran permukaan dentin secara signifikan.Kata kunci: Fluoride, kekasaran permukaan
Kemampuan Air Rebusan Daun Salam (Eugenia Polyantha Wight) Terhadap Jumlah Makrofag Pada Gambaran Histologi Periodontitis Agresif (Penelitian Pada Tikus Model) Ridha Andayani; Abdillah Imron Nasution; Afini Rahimi
Cakradonya Dental Journal Vol 8, No 2 (2016): Desember 2016
Publisher : FKG Universitas Syiah Kuala

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Abstract

Aggregatibacter actinomycetemcomitans merupakan bakteri dominan pada periodontitis agresif. Daun salam (Eugenia polyantha w) bersifat sebagai anti-inflamasi, antimikroba, analgesik dan antibakteri. Saat ini, belum banyak penelitian potensi daun salam dalam respon inflamasi yang diperankan oleh makrofag. Tujuan penelitian ini untuk mengetahui kemampuan air rebusan daun salam dengan konsentrasi 20%, 40%, dan 80% terhadap jumlah sel makrofag pada gambaran histologi periodontitis agresif. Penelitian ini merupakan penelitian eskperimental laboratoris yang menggunakan tikus putih rattus norvegiccus dibagi empat kelompok, tiga kelompok perlakuan air rebusan daun salam dengan konsentrasi 20%, 40%, dan 80%, dan satu kelompok kontrol akuades.Pada hari pertama kelompok perlakuan dan kelompok kontrol diinokulasi bakteri Aggregatibacter actinomycetemcomitans sampai hari ketujuh. Hari kedelapan sampai kesepuluh kelompok perlakuan diaplikasikan air rebusan daun salam dan kelompok kontrol diaplikasikan akuades. Hari kesebelas tikus dieuthanasi, selanjutnya dilakukan pengambilan sampel histologi jaringan periodontal tikus diamati secara mikroskopik. Hasil penelitian menunjukkan perbandingan jumlah makrofag kelompok perlakuan dengan kelompok kontrol menunjukkan perbedaan yang bermakna (p0,05). Dapat disimpulkan, kandungan air rebusan daun salam dapat membantu respon inflamasi yang diperankan oleh sel makrofag.
Fungistatic effect of Gracilaria verrucosa on phospholipase enzymes and the cell surface hydrophobicity of Candida albicans Gani, Basri A.; Andayani, Ridha; Batubara, Fitri Yunita; Ifwandi, Ifwandi; Syafriza, Dharli; Herlambang, Muhammad Mas'ud; Fathirah, Shiti Alya; Rifki, Ayudia
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 1 (2025): March
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i1.p66-73

Abstract

Background: Candida albicans (C. albicans) was reported as a primary cause of oral candidiasis. Phospholipase enzymes and cell surface hydrophobicity (CSH) are involved in the pathogenesis of its infection. Gracilaria verrucosa (G. verrucosa) is reported to contain flavonoids, tannins, and phenolic compounds, which can inhibit the development of C. albicans. Purpose: The study analyzed the effect of the ethanol extract of G. verrucosa on the inhibition of the phospholipase enzyme and CSH of C. albicans. Methods: The G. verrucosa chemical compounds were examined by phytochemical tests and phospholipase enzyme-inhibiting egg yolk media, and a CSH assay was conducted using xylene. Results: Gracilaria verrucosa contains steroids, terpenoids, tannins/phenolics, and flavonoids. The 25%–100% concentrations inhibit the phospholipase enzyme of C. albicans more strongly at 48 hours than at 12 hours and 24 hours (p < 0.05). Furthermore, the 100% and 75% concentrations substantially affect the inhibition of CSH of C. albicans, with a strong relationship. Conclusion: Gracilaria verrucosa has a more substantial inhibitory effect in suppressing the phospholipase enzyme and CSH of C. albicans.
PENGARUH KONSUMSI AIR MINUM REVERSE OSMOSIS (RO) TERHADAP LAJU ALIRAN, pH, DAN VISKOSITAS SALIVA PADA SISWA SMA NEGERI 10 FAJAR HARAPAN BANDA ACEH Santi Chismirina; Ridha Andayani; Afrina .; Putri Humaira Nanda Ibrahim; Hafizh Gunawan Amri
Journal of Syiah Kuala Dentistry Society Vol 4, No 1 (2019): OKTOBER
Publisher : Dentistry Faculty

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Abstract

One drink that is processed by the reverse osmosis method is Reverse Osmosis (RO) drinking water.Through this method, all micro particles contained in filtered water including essential minerals. Thereduction in essential minerals can reduce the activation function of salivary gland secretory cellsand it will have an impact on salivary flow, pH, and viscosity. This study aims to determine the effectof RO drinking water consumption to the flow rate, pH, and viscosity of saliva on students of FajarHarapan Senior High School Banda Aceh. The study subjects consisted of 45 subjects who consumedRO drinking water as the treatment group and 8 subjects who did not consume RO drinking water asa control group. Saliva from the treatment and control group subjects was collected by spittingmethod to measure the flow rate, pH, and viscosity of saliva. The results of the study that wereanalyzed using the unpaired T test showed that there was no effect of RO drinking water consumptionon the flow rate, pH, and viscosity of saliva in SMA Negeri 10 Fajar Harapan Banda Aceh. Based onthe results of the research, it can be concluded that the consumption of RO drinking water does notaffect the flow rate, pH and viscosity of the saliva in students of Fajar Harapan Senior High SchoolBanda Aceh. Keywords : Essential minerals, Calcium, Magnesium, Spitting method, pH meter.
HUBUNGAN STRES NEGATIF (DISTRES) DENGAN PERDARAHAN GINGIVA PADA PASIEN RUMAH SAKIT JIWA BANDA ACEH Sunnati .; Ridha Andayani; C Putroe Ula Rahifa; Sri Rezeki
Journal of Syiah Kuala Dentistry Society Vol 4, No 1 (2019): OKTOBER
Publisher : Dentistry Faculty

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Abstract

Gingival bleeding is an early feature of gingivitis. Gingivitis is characterized by gingival redness,swelling, and bleeding on probing. Gingival bleeding may be caused by local factor and systemicfactors. Negative stress (distress) is thought to be the risk factor of gingival bleeding. Previous studiesshowed that negative stress (distress) may contribute to the severity of gingival bleeding. However, insome cases heavy gingival bleeding was found in the presence of minimal plaque retention. Theobjective of this study was to see the correlation between negative stress (distress) and gingivalbleeding. This study was an analytic cross-sectional study taking Banda Aceh mental hospital. Thesubjects of this study were 58 patients aged between 15 60 years old. There were 30 male and 28female patients. Data was collected from medical record, history taking and clinical examination ofgingival bleeding and oral hygiene index-simplified (OHI-S). Statistic analysis using chi-square testrevealed that there was no correlation between negative stress (distress) to gingival bleeding (p0.05).Conclusion: This study shows that there is no significant correlation between negative stress (distress)to gingival bleeding. Keywords: Gingival bleeding, negative stress (distress), OHI-S
POTENSI EKSTRAK ALGA MERAH Gracilaria verrucosa SEBAGAI PENGHAMBAT PERKEMBANGAN PEMBENTUKAN BIOFILM Enterococcus faecalis PADA INFEKSI SALURAN AKAR GIGI Putri Rahmi Noviyandri; Ridha Andayani; Ervina Rizky
Journal of Syiah Kuala Dentistry Society Vol 3, No 1 (2018): JANUARY
Publisher : Dentistry Faculty

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Abstract

Enterococcus faecalis (E. faecalis) is a facultative anaerobic Gram-positive bacteria which becomesthe main bacteria occurrence failure of endodontic treatment. The red algae Gracilaria verrucosa (G. verrucosa) is one of the herbal plants that contain antibacterial compounds such as alkaloid,flavonoid, saponin, steroid, and triterpenoid. This study aims to determine the potency red algae G.verrucosa extract as inhibitor of E. faecalis biofilm formation development. The red algae G.verrucosa extract with a concentration of 100%, 75%, 25%, 12,5%, and 6,25% was made inmaceration method using ethanol 96%. Microtiter Plate Biofilm Assay method and ELISA readerwith wavelength 595nm were used to test the biofilm. The result of One Way ANOVA showedp0,05 which mean there was an effect of the test group on the formation of E. faecalis biofilm development and Post Hoc Tests-Duncan showed that there was a significant differences betweeneach group of those concentration. In the treatment group, there was found two concentration thathave significant differences, there were 100% and 6,25%. Based on the result of this study, we canconclude that the red algae G. verrucosa extract was useful as an inhibitor of E. faecalis biofilmformation development. The higher concentration of the red algae G. verrucosa, the higher its abilityas an inhibitor of E. faecalis biofilm formation development.Keywords : Enterococcus faecalis, red algae Gracilaria verrucosa, Microtiter Plate Biofilm Assaymethod, ELISA reader.
AKTIVITAS ANTIBAKTERI TEPUNG CACING TANAH (Lumbricus rubellus) TERHADAP Enterococcus faecalis SECARA IN VITRO Ridha Andayani; Zaki Mubarak; Dian Rizki Rinanda
Journal of Syiah Kuala Dentistry Society Vol 1, No 2 (2016): JULY
Publisher : Dentistry Faculty

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Abstract

AbstractEnterococcus faecalis is a facultative anerobic bacterium which can causesecondary periapicalinfection and is very resistant to numerous antimicrobial substances normally used during the rootcanal treatment. Earthworm (Lumbricusrubellus) possess antimicrobial peptide, known as Lumbricin-1 which is known tohinder the growth of Gram positive and Gram negative bacteria as well as fungi,but rarely caused resistance. This research was an experimental laboratory study conducted to observethe antibacterial activity of Lumbricin-1 contained in earthworm powder towards the growth of E.faecalis in vitro. Enterococcusfaecalis was cultured on CHROMagar VRE media and incubatedanaerobically for24-48 hours in the temperature of 37C. The bacterium was identified by observingthe colour of the colony of the bacterium growing on the CHROMagarVRE medium and Gramstaining, while antibacterial activity test was performedusing disk diffusion method. Statisticalanalysis using one way ANOVA and Duncan test showed that there was a significant difference (p 0,05) between test and control group. The result of the study showed that earthworm powderpossessed strong antibacterial activity towards the growth of Enterococcusfaecalis.Key words: Enterococcus faecalis, Lumbricus rubellus, antimicrobial peptide, Lumbricin-1
POTENSI ANTIBAKTERI EKSTRAK JAHE (Zingiber officinale Roscoe) TERHADAP Porphyromonas gingivalis SECARA IN VITRO Zulfan M. Alibasyah; Ridha Andayani; Ana Farhana
Journal of Syiah Kuala Dentistry Society Vol 1, No 2 (2016): JULY
Publisher : Dentistry Faculty

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Abstract

AbstractChronic periodontitis is the most common periodontal disease occurs in people with prevalence of96.58% and usually occurs at the age of 35 years and over. The main cause of chronic periodontitis isaccumulation of plaque and bacteria. The most dominant bacteria in chronic periodontitis isPorphyromonas gingivalis. Ginger (Zingiber officinale Roscoe) is a spice plant that is widely used bythe public, contain secondary metabolites such as flavonoids, terpenoids, phenols, and essential oilwhich is antibacterial. This study aim was to determined the gingers extract (Zingiber officinaleRoscoe) antibacterial potency against Porphyoromonas gingivalis with various concentrations are6.25%, 12.5%, 25%, 50%, and 100%. Antibacterial potency which test done by placing a paper discsthat has been dipped in the test group with 5 concentration and the placed on the surface of the MHAmedium (Mueller Hilton Agar) which had been grown Porphyromonas gingivalis, and incubated for 24hours at a temperature of 37 0C in an anaerobic atmosphere. Furthermore, inhibition zones werecalculated using calipers. Data were analyzed by one way ANOVA test which showed that the extractof ginger (Zingiber officinale Roscoe) can inhibit Porphyromonas gingivalis with probability (P0.05).Conclusion the extract of ginger (Zingiber officinale Roscoe) cold inhibit the growth ofPorphyromonas gingivalis at 6,25% concentration of extract with average of inhibition zone 10,6 mmthat categorized weak.Keywords: Ginger, Porphyromonas gingivalis, Chronic Periodontitis
Co-Authors Abdillah Imron Nasution Afini Rahimi Afrina . Amalia Dwi Habdani Amatu Sholiha Ana Farhana Andrian Andrian Basri A. Gani Basri A. Gani, Basri A. C Putroe Ula Rahifa Dian Rizki Rinanda Ervina Rizky Fajri Ekawardana Fathirah, Shiti Alya Fitri Yunita Batubara, Fitri Yunita Hafizh Gunawan Amri Herlambang, Muhammad Mas'ud Hessyi Amanda Pratiwi, Hessyi Amanda Ifwandi Ifwandi, Ifwandi Iga Kumalasari Iin Sundari Kartika Sari Kumalasari, Iga M. Naufal Asyraf Mauliza Hanim Melati Hayatul Husni, Melati Hayatul Muhammad Qadri mulkhairul, Ilham Niken Yulia Novriyanti Fatimah Harahap, Novriyanti Fatimah Putri Disa Maulida Putri Humaira Nanda Ibrahim Putri Rahmi Noviyandri Rani Nurzaini Harahap Ratih Asrina Fitri Rifki, Ayudia Rosdiana Ginting Santi Chismirina Sri Rezeki Sunnati . Suzanna Sungkar, Suzanna Syafriza, Dharli Ulfah, Khairiyah Vivi Zayanti Zaki Mubarak Zulfan M. Alibasyah