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Induksi dan Multiplikasi Kalus Eucalyptus sp. pada Berbagai Media Callus Induction Medium (CIM) Secara In Vitro BARAELA EZRA WIJAYA; RINDANG DWIYANI; NI NYOMAN ARI MAYADEWI
Agrotrop : Journal on Agriculture Science Vol 13 No 1 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i01.p07

Abstract

Callus Induction and Multiplication of Eucalyptus sp. on Various Media Callus Induction Medium (CIM) In Vitro. Callus induction in vitro can be done by planting specific part of plant such as leaf, root, stem, etc in aseptic condition by using medium that contains 2,4-D, a plat regulator for promoting callus formation. One of the objectives of callus production in vitro is to form secondary metabolites, because eucalyptus is known as a producer of essential oils. This research is an initial study for in vitro production of secondary metabolites for further research. The aim of this study was to determine the most suitable callus induction medium for callus formation and callus multiplication for Eucalyptus grown in vitro. This research was designed as a Completely Randomized Design (CRD), five treatments, repeated five times. Woody Plant Medium (WPM) was used as the basic media. Several Callus induction Medium (CIM) were used as treatments, i.e. CIM1 = 1 ppm 2,4-D; CIM2 = 1,5 ppm 2,4-D; CIM3 = 2 ppm 2,4-D; CIM4 = 1 ppm NAA+1 ppm BAP; and CIM5 = 2 ppm NAA+ 2 ppm BAP. Variables observed were the time of curly leaf formation, time of the emerging callus, colour and texture of the callus, callus’s weight, callus surface area, the weight of callus after multiplication. The result showed that CIM4 and CIM5 had the fastest emerging callus (7 days after planting), CIM1 and CIM2 formed green and crumb texture of callus, CIM1 produced the highest of callus’s weight, and CIM2 resulted in the highest of callus’s surface area. In conclusion, the most suitable CIM for callus induction was CIM1, while for callus multiplication was CM2.
Identifikasi Karakter Morfologi dan Analisis Kandungan Nutrisi Buah Pisang Mas, Buluh, dan Lumut Lokal Bali I GUSTI AYU ARINI LAKSEMI; I NYOMAN RAI; NI NYOMAN ARI MAYADEWI
Agrotrop : Journal on Agriculture Science Vol 13 No 1 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i01.p03

Abstract

Identification of Morphological Characters and Fruits Nutrient Analysis of Pisang Mas, Buluh and Lumut Local Bali. Banana are a common commodity in Indonesia, having high economic, cultural and nutritional value. The objective of this research was to find the difference of morphological characters and nutrient content of Pisang Mas, Buluh and Lumut Local Bali. The research was conducted from July to October 2021. The samples of banana were taken from Antap Village, Selemadeg Barat District, Tabanan Regency, while identification and analysis were conducted in the laboratory. This research used UPGMA analysis and randomize complete design (RCD) with 3 replications. The results of identification showed different qualitative morphological characters in bunch position, fruit shape, fruit apex, remains of flower relicts at fruit apex, mature peel color, pedicel surface, pulp color before maturity, pulp color at maturity and predominant taste, while the quantitative showed the different in the number of pedicels, fruit length, fruit pedicel length and fruit peel thickness. Pulp nutrient content showed differences in water content, dry matter, crude lipid, crude fiber, and carbohydrate content.
Innovative Application of Onion Extract to Improve the Quality of Tomato Fruit Ni Nyoman Ari Mayadewi; I Nyoman Gede Astawa; Anak Agung Keswari Krisnandika; Ni Made Classia Sukendar
SOCA: Jurnal Sosial Ekonomi Pertanian Vol 17 No 1 (2023): Vol 17 No 1 (2023)
Publisher : Program Studi Agribisnis, Fakultas Pertanian, Universitas Udayana Jalan PB.Sudirman Denpasar, Bali, Indonesia. Telp: (0361) 223544 Email: soca@unud.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/SOCA.2023.v17.i01.p05

Abstract

Tomatoes are one of the daily necessities for people. In general, consumers favor tomatoes with few seeds. Therefore, there must be efforts made to improve the quality of tomatoes, one of which is to reduce the number of seeds, even if it is possible to make tomatoes without seeds (seedlessness). By applying Gibberellic acid (GA) during the growth phase of tomato plants, it is possible to enhance tomato quality through technology. The application of GA to certain plants can reduce the number of tomato seeds and increase their sweetness. It is necessary to discover a way to use GA-containing natural ingredients to prepare for the high cost of synthetic GA. Onions are one of the natural ingredients that contain GA. Thus, this study aimed to examine the effect of applying onion extract as a source of GA to obtain the optimum concentration of onion extract that can improve the quality of tomato fruit. The experimental design utilized Randomized-Group Design with five different treatments: no spraying, spraying with 25%, 50%, 75%, and 100% onion extract. According to the findings, the application of onion extract improved the quality of tomato fruit. This condition is reflected by a decrease in the number and weight of seeds per fruit and an increase in the fruit's vitamin C content. Therefore, it is essential to conduct this research to produce inexpensive, high-quality tomatoes to sustain the economy and increase farmers' income
The Effect of Application of Diplazium Esculentum Leaf Extracts and GA3 on the Berry Quality of Grape (Vitis Vinifera L.) Cultivar of ‘Prabu Bestari’ I Nyoman Gede Astawa; Rindang Dwiyani; Ni Nyoman Ari Mayadewi; Prila Kartika Manulang; Anak Agung Made Astiningsih
Agro Bali : Agricultural Journal Vol 6, No 2 (2023)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v6i2.1218

Abstract

This study investigated the effect of extract of Diplazium esculentum leaves and GA3 on the berry quality of Vitis vinifera c.v. Prabu Bestari. The objective of this research was to find out the effect of the application of D. esculentum leaf Extract and GA3 on the berry quality of grape c.v. Prabu Bestari. This study was conducted in the Vineyard at the Experimental Station of the Faculty of Agriculture, Udayana University during April to October 2021. A total of five different treatments were applied on inflorescences of grape (by dipping them), i.e. T0= control, only distilled water; T1 = 200 cc leaf extract was diluted with distilled water in to 1 L (200 cc L-1); T2=400 cc L-1; T3 = 600 cc L-1; T4= 500 ppm GA3 (as control positive). There were five treatments arranged in a randomized block design with 6 repetition.  The results showed that all treatments were not able to induce seedless, as seeds still presented inside berries, however, the number of seeds in the treatment of 500 ppm GA3 reduced significantly compared to other treatments.  Weight per seed decreased significantly at 400 cc L-1 D. esculentum extract, 600 cc L-1 D esculentum extract and 500 ppm GA3.
RESPON PERTUMBUHAN TANAMAN PAKCOY (Brassica rapa L.) PADA BERBAGAI JENIS MEDIA TUMBUH DALAM SISTEM BUDIDAYA VERTIMINAPONIK I Nyoman Wahyu Ardianta; I Nyoman Rai; Ni Nyoman Ari Mayadewi
Plumula : Berkala Ilmiah Agroteknologi Vol. 9 No. 2 (2021): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vertiminaponik merupakan perpaduan antara budidaya tanaman (hidroponik) dan budidaya ikan (akuakultur) dalam satu tempat/lahan yang disusun secara vertikal. Dalam sistem vertiminaponik media tanam memegang peranan penting sebagai salah satu penentu pertumbuhan tanaman dan ikan. Tujuan penelitian ini adalah mengkaji media tanam terbaik untuk pertumbuhan dan hasil pakcoy serta pertumbuhan dan hasil ikan lele pada sistem vertiminaponik. Penelitian menggunakan rancangan acak kelompok (RAK) dengan satu faktor yaitu media tanam yang terdiri atas empat taraf, yaitu pecahan batu bata, pasir malang, kerikil dan zeolit. Hasil penelitian menunjukkan, media tanam pasir malang menghasilkan berat kering total dan berat segar total hasil panen pakcoy tertinggi yaitu 12,42 g dan 240,72 g. Media tanam zeolit memberikan hasil bobot ikan lele tertinggi yaitu 11,75 kg, namun berbeda tidak nyata dengan media pasir malang (11,03 kg).
Identifikasi Karakter Morfologi dan Analisis Kandungan Nutrisi Buah Pisang Susu, Kepok, dan Raja Lokal Bali Liran, I Gusti Putu Oka Maha Putra Wardana; Rai, I Nyoman; Mayadewi, Ni Nyoman Ari
Agrotrop : Journal on Agriculture Science Vol 14 No 1 (2024)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Identificaion of Morphological Characters and Fruits Nutrient Analysis of Pisang Susu, Kepok, and Raja Lokal Bali. Banana (Musa sp.) is one of the germplasms which is distributed in tropical and sub-tropical areas. Various types of bananas exist, causing differences in character between each cultivar. This research was carried out in July - October 2022. The location for sampling bananas was in Banjar Soka, Antap Village, Selemadeg Barat District, Tabanan Regency, while morphological identification and analysis of nutrient content were carried out at Laboratory. This study used UPGMA analysis and a completely randomized design (CRD) with three replications. Identification of morphological characters revealed differences in the qualitative characters of the shape of fruit, shape of the tip of fruit, rest of the flower relics, colour of the peel before and after ripening, cracking of the peel, surface of the peels, colour of pulp before and after ripening shape of the seeds, finger length, finger stalk length, finger stalk width and fruit peel thickness. The nutritional content of the fruit flesh is different in crude fat and crude fibre.
Pengaruh Kadar Air Tanah dan Jumlah Tanaman Inang Berbeda terhadap Perbanyakan Spora FMA dan Pertumbuhan Tanaman Jagung Fau, Maria Berlian; Rai, I Nyoman; Wiraatmaja, I Wayan; Mayadewi, Ni Nyoman Ari
Plumula : Berkala Ilmiah Agroteknologi Vol. 12 No. 2: Juli 2024
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v12i2.228

Abstract

Tanaman jagung merupakan jenis tanaman yang paling umum digunakan sebagai inang (host) dalam perbanyakan spora mikoriza. Penelitian ini bertujuan untuk mengetahui metode perbanyakan Fungi Mikoriza Arbuskula (FMA) dengan kadar air tanah dan jumlah tanaman inang dan pengaruhnya terhadap pertumbuhan tanaman jagung. Rancangan percobaan yang digunakan, yaitu RAK 2 faktor. Faktor pertama kadar air tanah terdiri atas 4 taraf yaitu K1 (100% kapasitas lapang), K2 (75% kapasitas lapang), K3 (50% kapasitas lapang) dan K4 (25% kapasitas lapang). Faktor kedua jumlah tanaman inang per pot terdiri atas 3 taraf, yaitu J1 (1 tanaman per pot), J2 (2 tanaman per pot), dan J3 (3 tanaman per pot). Hasil penelitian menunjukkan bahwa interaksi perlakuan kadar air 25% dan jumlah tanaman tiga per pot (K4J3) menghasilkan 372.275 spora per pot. Pada pertumbuhan tanaman jagung, faktor tunggal kadar air tanah K3 menghasilkan berat kering total tertinggi, yaitu 3.065 g, dan faktor tunggal jumlah tanaman inang per pot J3 menghasilkan berat kering total tertinggi, yaitu 6.445 g.
Indigenous arbuscular mycorrhizal fungi at tangerine cv. Tejakula (Citrus reticulata cv. Tejakula) plantations, their colonization of the roots, and their effect on soil fertility Wijana, Gede; Rai, I Nyoman; Mayadewi, Ni Nyoman Ari; Astiari, Ni Komang Alit
Journal of Degraded and Mining Lands Management Vol. 12 No. 2 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.122.7173

Abstract

Tangerines (Citrus reticulata var. Tejakula) were destroyed in 1984 by CVPD, allegedly because the use of chemicals was too intensive, resulting in land degradation. Since ten years ago, these oranges have been successfully cultivated again using healthy organic cultivation, but farmers have difficulty getting organic fertilizer. This research aimed to identify and isolate the Arbuscular Mycorrhizal Fungi (AMF) on tangerines cv. Tejakula, their colonization of the roots and effect on soil fertility. Exploration was conducted by collecting root and soil samples in tangerine cv. Tejakula production centers are in the Tejakula District, Buleleng Regency. Spore isolation was conducted using wet filtration and centrifugation techniques, while root colonization by AMF was carried out using the staining method with trypan blue. The isolated spores are composited into AMF biofertilizer using volcanic sand as a carrier medium. The biofertilizer was then tested for its effect on soil fertility with three treatment levels of AMF spore doses (0, 20, and 40 spores/50 g of carrier media per plant). The results of exploration, isolation, and identification showed that three genera and six species of AMF were found. The spore density ranged from 17 to 32 per 100 g of soil (mean 24.17 ± 2.02 spores). The average root colonization by AMF was very high, ranging from 90 to 100%, and the application of AMF biofertilizer at a dose of 20 and 40 spores per plant provided soil fertility was much better than the control (0 AMF spores per plant).
Remediation of heavy metal-contaminated paddy soils using biochar and its effect on corn growth and yield Mayadewi, Ni Nyoman Ari; Rai, I Nyoman; Indrayuni, Dewa Ayu Bulan
Journal of Degraded and Mining Lands Management Vol. 12 No. 4 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.124.8325

Abstract

Badung River, a primary irrigation source for the subak system in South Denpasar, has been reported to contain excessive levels of heavy metals, exceeding the standard quality for irrigation water. Corn cultivated on subak systems irrigated by this irrigation source had heavy metal residues in its roots and kernels. Long-term consumption of corn containing heavy metals such as Pb, Cd, Cu, Cr, and Hg will cause toxicity to plant tissues and the human body. This study examined the content of heavy metals in soil and corn plants in subak systems in South Denpasar, which are irrigated with water from the Badung River. Remediation of the soils contaminated by heavy metals was conducted using rice straw and husk biochar (Br), coconut coir and shell biochar (Bc), vegetable and fruit waste biochar (Bw), and lignohumic biochar (Bl). The study also evaluated the effect of applying biochar on growth and yield of corn grown on soils contaminated by heavy metals. The results of this study showed that the heavy metal contents in the soil and irrigation exceeded the threshold for soil and water quality standards. The application of Bl yielded the highest biomass fresh weight and seed weight at 14% moisture content compared to other treatments. The Bl treatment also resulted in the best performance in binding and translocating heavy metals based on the bioaccumulation and translocation values ??of the biochar types. Hence, it can be concluded that the most suitable biochar for the remediation of heavy metal-contaminated paddy soils in subak systems is lignohumic biochar.