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Pemanfaatan Bakteri Pseudomonas Flourescens Rh4003 dan Asam Askorbat untuk Mempertahankan Viabilitas Benih Padi Hibrida A. A. Keswari Krisnandika; Eny Widajati; Abdjad Asih Nawangsih
Buletin Agrohorti Vol. 5 No. 2 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.619 KB) | DOI: 10.29244/agrob.v5i2.16800

Abstract

Padi hibrida memiliki glume terbuka yang berperan penting pada laju perlambatan viabilitas benih selama penyimpanan. Serta untuk mencapai hasil panen padi hibrida yang optimal membutuhkan asupan nutrisi yang berkualitas tinggi. Penelitian ini bertujuan untuk mempelajari pengaruh formula lapisan benih dengan penambahan bakteri Pseudomonas fluorescens RH4003 sebagai Pertumbuhan Tanaman Memnghasilkan Rhizobakteri dan asam askorbat sebagai antioksidan, menghambat viabilitas benih padi hibrida dalam penyimpanan. Penelitian ini terdiri dari tiga seri percobaan, yaitu menggunakan 3 varietas padi hibrida (DG-1, Intani dan SL-8), Rancangan percobaan yang digunakan yaitu petak bersarang dengan enam kali periode penyimpanan sebagai petak utama. Hasil penelitian menunjukkan bahwa lapisan yang menggunakan asam askorbat merupakan perlakuan terbaik dari semua variabel viabilitas hampir pada semua varietas. Perlakuan dengan menggunakan bakteri memiliki hasil yang signifikan (p < 0.01) meningkatkan laju pertumbuhan dan indeks vigor pada minggu keenam penyimpanan sebesar 22.48% per etmal dan 83.33% untuk varietas benih padi SL-8. Lapisan DG-1 yang menggunakan asam askorbat pada minggu keenam mengakibatkan indeks vigor tertinggi (90 %) yang signifikan (p<0.5) dan peningkatan yang sangat signifikan pada perkecambahan benih dan indeks vigor minggu kesembilan penyimpanan untuk varietas SL-8 SHS (92.67% dan 73.33%).
Pelet Bakteri Probiotik untuk Biokontrol Xanthomonas oryzae pv. oryzae dan Viabilitas Benih Padi Anak Agung Keswari Krisnandika; Eny Widajati; Wawan Hermawan; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 12 No 1 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.369 KB) | DOI: 10.14692/jfi.12.1.27

Abstract

Probiotic bacteria collection from the rice plant tissue (i.e. endophytic 467 and endophytic 748 isolates), the rhizosphere (Ralstonia pickettii TT47) and that from the ground (actinomycetes 6) has been reported as biocontrol agents. The effect of pellet containing probiotic bacteria to suppress Xanthomonas oryzae pv. oryzae pathogen in rice seed has not been known thoroughly. This research was carried out to evaluate the effect of pellet containing probiotic bacteria in maintaining viability of rice seeds infected by X. oryzaepv. oryzae. A dual culture method was used to test the antagonistic activities between probiotic bacteria and X. oryzaepv. oryzae. Isolates R. pickettii TT47, endophytic 467 and actinomycetes 6 showed antagonistic activities against X. oryzae pv. oryzae. Among them, only endophytic 467 and actinomycetes 6 that showed compatibility. Pellet formulation (talc + CMC 1.5% + glycerol 1%) contain actinomycetes 6 singly or combination with endophytic 467 in infected Ciherang rice seed was able to suppress population X. oryzaepv. oryzae as long as 6 weeks storage. While, the highest percentage of seed germination and seedling growth rate during 6 weeks storage was obtained on pellet formulation with R. pickettii TT47, i.e. 86.67% and 17.17% etmal-1 respectively and significantly different with infected nonpelleted rice seed (62.67% and 11.02% etmal-1). In conclusion, the application of probiotic bacteria R. pickettii TT47, endophytic 467 and actinomycetes 6 in pellet formulation was effective to decrease X. oryzaepv. oryzae and maintain viability of infected rice seed in 6 weeks storage.
Model Desain Taman Toga Desa Bukian, Kecamatan Payangan, Kabupaten Gianyar Wiranatha Kadek; I Made Agus Dharmadiatmika; Anak Agung Keswari Krisnandika
Jurnal Lanskap Indonesia Vol. 13 No. 2 (2021): Jurnal Lanskap Indonesia
Publisher : http://arl.faperta.ipb.ac.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jli.v13i2.34321

Abstract

Tanaman Obat Keluarga (Toga) adalah sebidang tanah di halaman rumah yang dimanfaatkan untuk menumbuhkan tumbuhan yang berkhasiat obat untuk memenuhi kebutuhan obat keluarga. Nilai fungsional taman Toga di pekarangan rumah Desa Bukian sudah tercapai namun penataan tamannya belum mempertimbangkan karakter tanaman, estetika serta filosofi Taman Tradisional Bali. Konsep dasar yang diterapkan adalah taman tematik yang menyeimbangkan antara keindahan taman dengan suplay tanaman obat, berdasarkan filosofi taman tradisional bali yang terdiri dari 7 unsur pembentuk taman di Bali. Yang ditonjolkan dari konsep ini adalah tata ruang sebagai alas, atap, dinding dan pola desain di masing-masing mandala yang saling terkait menjadi satu kesatuan sehingga mampu menjadi panduan pemilik rumah dalam menata taman, serta rekomendasi model desain taman Toga hasil dari penempatan tanaman pada konsep ruang yang telah dirancang berdasarkan karakter masing-masing tanaman dan menjadikan filosofi taman tradisional bali sebagai panduan. Dalam setiap perancangan taman di pekarangan diharapkan menggunakan filosofi tamana tradisional bali sebagai dasar sehingga dapat menjaga dan melestarikan kearifan lokal dan menjaga kesehatan keluarga dengan pengobatan herbal.
Pengaruh Kombinasi Kerapatan Kanopi Pohon terhadap Kenyamanan Termal di Lapangan Puputan Margarana, Denpasar Bagas Utomo Putra; Anak Agung Keswari Krisnandika; I Made Agus Dharmadiatmika
Jurnal Lanskap Indonesia Vol. 14 No. 1 (2022): Jurnal Lanskap Indonesia
Publisher : http://arl.faperta.ipb.ac.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jli.v14i1.38646

Abstract

Puputan Margarana as a city park is used as a public space that can accommodate outdoor activities for its users. Comfort is an important factor consider for users to conduct activity inside. User comfort level can be measured using the thermal humidity index formula based on the temperature and humidity variables. The temperature and humidity can be influenced by the presence of vegetation, especially trees. Differences in tree species composition will cause differences in the canopy density formed. This study aims to identify the effect of the combined tree canopy density on thermal comfort. This research method begins with determining the sample point based on the combination of canopy density to areas that are not shaded by the canopy. Then, the canopy was photographed at the sample point for analysis using Hemiview 2.1 software to obtain the leaf area index (LAI) value. Furthermore, independent samples T test, assessment of thermal comfort, correlation analysis and linear regression between variables. The results of the analysis show that there is a strong correlation between LAI with temperature, humidity, and THI. There were significant differences between THI at sample points 1, 2, 4, and 5 with LAI 1.68-4.53 against THI without canopy shade. Meanwhile, THI at sample points 3 and 6 with LAI 0.96-1.36 was not significantly different from THI without canopy shade. This shows that the combination of tree canopy density in this study has an effect on thermal comfort. This shows that canopy density can be one of the considerations in selecting trees for landscape design for climate engineering purposes.
Social Economy and Digital Mapping of Subak Sembung Ecotourism Gede Mekse Korri Arisena; Anak Agung Keswari Krisnandika; Dwi Putra Darmawan
Agriekonomika Vol 9, No 2: Oktober 2020
Publisher : Department of Agribusiness, Faculty of Agriculture, Universitas Trunojoyo Madura, Indonesi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agriekonomika.v9i2.8754

Abstract

The social, cultural, economic, and environmental potentials in Subak Sembung are not well-developed, such that these potentials have not been adequately explored by the local community. This study aimed to describe the synergy between subak and the ecotourism in supporting the sustainable national food stability program. Subak Sembung selected as the study location because its location in the middle of Denpasar City and already developed as ecotourism sites. There were 66 participants involved in this study. Formal surveys, rapid appraisal, and map-making through satellite imaging from the Google Earth Pro (GE) application were used to collect the data. Result revealed that the social economy social activities conducted by the farmer were: (1) seed-producing tools, fertilizers, pesticides, agricultural tools, (2) land-tillage, nurseries, cultivating, preserving the plant, harvesting, (3) marketing, and (4) the correlation between farmers, subak and ecotourism; worth to be developed as a tourist attraction site.
KENAL KEBUN UNTUK ANAK USIA DINI N. Kohdrata; C.G.A. Semarajaya; A.A.K. Krisnandika; L.S. Yusiana; A.A.A.W.S Djelantik
Buletin Udayana Mengabdi Vol 17 No 4 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.512 KB) | DOI: 10.24843/BUM.2018.v17.i04.p25

Abstract

Pengabdian dengan judul Kenal Kebun pada Anak Usia Dini berangkat dari pemikiran untuk membuat suatu program untuk mengenalkan alam lingkungan sejak usia dini pada anak-anak usia pra sekolah. Mencari sepetak taman atau kebun di fasilitas pendidikan di wilayah perkotaan pada saat ini, khususnya Denpasar, merupakan hal yang tak mudah. Bahkan lingkungan sekolah anak-anak PAUD Permata Bunda yang menjadi sekolah untuk program pengabdian juga sangat terbatas dalam hal ketersediaan ruang hijau. Fakultas Pertanian Universitas Udayana memiliki Kebun Percobaan yang berlokasi di Jl. Pulau Moyo, Denpasar, seluas 1,4 ha kiranya dapat menjadi wahana untuk pengenalan aktifitas kebun yang tepat kepada anak-anak. Metode pelaksanan adalah hands-on learning.Anak-anak usia dini akan diajak untuk mengenali beberapa tanaman kebun dan juga serangga sebagai bagian dari pengenalan langsung. Pengalaman langsung ini diharapkan makin mendekatkan mereka dengan lingkungan alami disamping menambah pengetahuan mereka akan dunia sekitar. Anak-anak diajak untuk mengenal nama-nama tanaman dengan melihat dan memegang secara langsung benda yang dimaksud.
Karakteristik Molekuler Virus Avian Orthoavulavirus 1 Genotipe VII yang Diisolasi dari Tabanan Bali (MOLECULAR CHARACTERISTIC OF AVIAN ORTHOAVULAVIRUS 1 GENOTYPE VII ISOLATED FROM TABANAN BALI) Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Nengah Wandia; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Anak Agung Keswari Krisnandika; Anak Agung Oka Wijaya
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.964 KB) | DOI: 10.19087/jveteriner.2019.20.4.593

Abstract

Newcastle disease (ND) is a very harmful avian disease, endemic in Indonesia and various parts of the world. The causative agent is ND virus or Avian orthoavulavirus 1 (AOAV-1). This virus is an RNA virus with wide genetic variation. Based on the genome length, it can be classified into AOAV-1 Class I and II. Class I are generally avirulent whereas Class II are consist of both virulent and avirulent viruses, currently there are 18 genotypes of the class II. To find out the molecular characteristics of AOAV-1 currently circulating in the field, isolation and identification of viruses from laying hens that was suspected ND from Tabanan Bali in 2017, was performed. The isolated viruses hereafter named as Tabanan1/ARP / 2017. A one-step RT-PCR reaction was carried out to amplify NP, F and HN gene fragments from the virus using three specific pairs of AOAV-1 primers. The obtained nucleotide sequences are then used in phylogenetic analysis. For phylogenetic analysis several strains of AOAV-1 from class II representing genotype I-VII as well as one strain from Class I were accessed from GenBank. From the analysis of the F gene nucleotide sequences, it was found that Tabanan 1 / ARP / 2017 is a genotype VII virus with an amino acid sequence at the F protein cleavage site is 112 R-R-Q-K-R-F117, a typical virulent strain. Phylogenetic analysis using nucleotide sequences NP and HN genes also positioned this isolate in genotype VII. At the nucleotide level, genetic distance with virulent isolates that was isolated in 2007 and 2010 were 8.26% and 1.08% while at the amino acid level were 5.26% and 0.64%. There were found mutations in amino acids at positions 107 and 108 of F protein.
Identifikasi tanaman penyerap Pb di tiga ruas jalan Kota Denpasar Anak Agung Keswari Krisnandika; Naniek Kohdrata; Cokorda Gede Alit Semarajaya
Jurnal Arsitektur Lansekap Vol.5, No.2, Oktober 2019
Publisher : Prodi Arsitektur Pertamanan, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.963 KB) | DOI: 10.24843/JAL.2019.v05.i02.p10

Abstract

Plant leaf capability identification to absorb Pb in three sections of Denpasar city streets. Plant as a softelement on landscape’s road, can also function as a pollutant absorber. Plants ability to absorb pollutants,depending on their characters, especially leaves morphology and its environment. Gajah Mada, P. B. Sudirmanand Letda Tantular are three roads in Denpasar City which have highly activity of human and vehicle based ontheir location in center of trade, education and government area. Humans in this area potentially risk ofexposure to lead (Pb) of motor vehicle emissions. One solution to minimize Pb exposure to humans is to useplants that can absorb dust and Pb along those roads. Survey's result shows that the dominant plants in LetdaTantular roadside are Lagerstroemia speciosae and Samanea saman, while Plumeria rubra and Callistemonviminalis are dominant in Gajah Mada, at P. B. Sudirman, Cerbera manghas and Polyalthia longifolia are dominating. The leaf of those plants then analyzed to know their capability in accumulate dust and Pbabsorption. Laboratory test showed that Lagerstroemia speciosae leaves have the best ability to absorb dust(5.80472 mg/cm2) and absorb Pb (5.04133 mg/kg). Morphology’s character of Lagerstroemia speciosae leavesis stiff, with a rough surface but not hairy. Lagerstroemia speciosae has row and dense canopies with moderateplant height, this plant roots also didn't damage roads. Therefore, Lagerstroemia speciosae is one of the properplant to use as pollutant absorber especially on fairly large roads with dense human activities.
Pendekatan Fisik dan Ekologis Penggunaan Pohon Asam Jawa Sebagai Tanaman Tepi Jalan di Sekeliling Trotoar Lapangan Puputan Badung, Denpasar NI WAYAN FEBRIANA UTAMI; ANAK AGUNG KESWARI KRISNANDIKA
Jurnal Arsitektur Lansekap Vol.2, No.2, Oktober 2016
Publisher : Prodi Arsitektur Pertamanan, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1021.165 KB) | DOI: 10.24843/JAL.2016.v02.i02.p08

Abstract

ABSTRACTPhysical and Ecological Approaches to Using Tamarinds as Street Trees around the Sidewalk in Puputan Badung Parks, DenpasarMany streetscapes in Indonesia cities used tamarinds (Tamarindus indica L.) tress as soft scape elements and Denpasar city is also one of them. This paper is concerned with the use of tamarinds species in street trees, especially it uses around the Puputan Badung sidewalks. The objectives of this paper were to identify the positive impact of tamarinds physically and ecologically on the environment. A survey methods was employed to obtain information about the existing trees. Descriptive methods was used to analyze trees character physically and ecologically and also its suitability based on literature study. Study results showed that there were sixteen individuals of tamarinds found along the sidewalk in Puputan Badung parks. The trees distributed as many as four individuals on northern and southern part, six individuals on eastern part, and two individuals on western part of the park. Some physical characteristic of the trees have been identified, i.e. vase canopy shape to provide tree canopy cover and avoid sun glare effect, fine plant texture to expand space impression, and slow stem growth. In addition, trees ecological characteristics recognized for controlling air pollutants (15N and Pb) and also providing wildlife habitat for lac insects (Laccifer lacca) as well as providing nectar for honeybees. It is suggested that, given the contribution of tamarind trees to the environment, more and more of them should be planted as street tress, and to maintain it, the requirements of planting the trees should be taken into account.
Karakter pengunjung dan radius layanan taman kota:studi kasus Taman Puputan Badung I Gusti Ngurah Made Agung, Denpasar Naniek Kohdrata; Anak Agung Keswari Krisnandika; Cokorda Gede Alit Semarajaya
Jurnal Arsitektur Lansekap Vol.6, No.2, Oktober 2020
Publisher : Prodi Arsitektur Pertamanan, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JAL.2020.v06.i02.p13

Abstract

City Park Service Distance: Case Study of Puputan Badung I Gusti Ngurah Made Agung Denpasar Park. This research is a preliminary work on finding out how a park has been functioned as a public facility to a city. It is aimed to find out Lapangan Puputan Badung service distance range as one of Denpasar city parks. Moreover, it is also to find out the characteristic uses of the park by its users. This case study uses a survey research method. Sample of respondents are chosen using accidental sampling technique with questionnaire as media of data collection. Research findings are analyzed spatially and descriptively as well. Result shows that most samples live within 5 km range from the park. The park has been used mostly for recreation and exercises. People within productive age have been dominated the use of the park. Duration people spend in the park is between 1 – 2 hours, however there is an interesting finding that 15% samples say that they spend 3 – 4 hours in the park. There is also a strong tendency that people uses the park as a group, meaning they might be there with family, relatives, or friends. More than 50% of user respondents are the park frequentvisitors. As conclusion, it can be stated that Lapangan Puputan Badung function the best to serve the dwellers within 5 km radius.