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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia AGRITEKNO, Jurnal Teknologi Pertanian Community Development Journal: Jurnal Pengabdian Masyarakat Rumphius Pattimura Biological Journal BAKIRA: Jurnal Pengabdian Kepada Masyarakat Kalwedo Sains (KASA) BAKTI : Jurnal Pengabdian Kepada Masyarakat
Pagaya, Joseph
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Social Sciences Other

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Journal : Rumphius Pattimura Biological Journal

 1 
THE EFFECT OF SEVERAL TOOTHPAST ON THE GROWTH OF Streptococcus mutans Nawaly, Hermanus; Pagaya, Joseph; Kaihena, Martha
RUMPHIUS Vol 2 No 1 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i1p011-018

Abstract

This study aims to determine the effect of several toothpastes on the growth of Streptococcus mutans which is the main cause of tooth decay. The study used 5 types of toothpaste, namely toothpaste A, toothpaste B, toothpaste C, toothpaste D, and toothpaste E. The antibacterial testing method used the diffusion method with the cup-plate technique. The significance level of each type of toothpaste was measured using a completely randomized design followed by a Tukey test to see differences in toothpaste treatment. The results showed that toothpaste A with active ingredients Monofluorophosphate Sodium 1.12%, Triclosan 0.3%, Calcium Glycerophosphate 0.13% had the largest average resistance zone of 27.3 mm. Whereas Toothpaste E with the active ingredient 0.08% Sodium Monofluorophosphate, 0.01% Sodium Fluoride has the smallest average resistance zone, which is 14.25 mm. The diameter of the ANOVA test zone of several types of barrier toothpaste against Streptococcus mutans at 95% confidence intervals showed that there was a significant difference.
ANTIMICROBIAL POTENTIAL OF GARLIC FRESH (Allium sativum L.) AND LAKOR ONION (Allium ascalonicum L.) ON THE GROWTH OF Staphylococcus aureus Pagaya, Joseph; Lysay, Claudia Priscia; Seumahu, Cecillia Anna
RUMPHIUS Vol 3 No 2 (2021): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv3i2p030-033

Abstract

Garlic (Allium sativum L.) and shallot Lakor (Allium ascalonicum L.) local products are plants that are used as spices for various dishes and can be used as herbal medicine. For natural use, safe and easy to consume which can be seen through its potential as herbal medicine, a study was carried out to test the antimicrobial potential of garlic (Allium sativum L.) and Lakor shallot (Allium ascalonicum L.) juice on the growth of bacteria (Staphylococcus aureus). Lakor garlic and shallots are grated, the results of grated Lakor garlic and shallots are squeezed to produce 100% pure onion bulb juice. Lakor garlic and shallot juice was diluted with concentrations of 25%, 35%, 45%, 55%, 65%, 75%, 85% and 95%, respectively. Antimicrobial activity was tested by the diffusion method using a disk to measure the diameter of the clear zone. The results showed that garlic (Allium sativum L.) was able to inhibit the growth of Staphylococcus aureus which could be demonstrated by the clear zone diameter of 16.3 mm at a concentration of 35% and at a concentration of 65% the diameter of the clear zone exceeded the clear zone of the amoxycillin antibiotic. Lakor red onion (Allium ascalonicum L.) showed antimicrobial potential but was unable to damage the bacterial cell wall as indicated by the diameter of the clear zone of 7.7 mm at a concentration of 95%. There is potential for both onion bulbs but have different antimicrobial properties against the growth of Staphylococcus aureus.
IN VITRO ANTAGONISM OF THE ISOLATE MUSHROOM Trichoderma harzianum TO Fusarium oxyzporum f. sp. Cubense CAUSES WILT DISEASE IN BANANA KEPOK (Musa paradisiaca normalis) Pattikawa, Apriyanti Santi; Pagaya, Joseph; Patty, Jogeneis
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p050-055

Abstract

Trichorderma harzianum is a soil native fungus that is beneficial because it has high antagonistic properties against plant pathogenic fungi. Control mechanisms can be: antibiosis, parasitism, competition and hyphae interference. T. harzianum as a biological control agent for the pathogenic fungus Fusarium oxyforum f. sp. Cubense is an alternative to control this pathogenic fungus, without causing a negative impact on the environment. The aim of the study was to determine whether Trichodernma harzianum isolates Piru, Kairatu and Uraur could suppress the growth of the fungus Fusarium oxyforum f. sp. cubense and Trichorderma harzianum which isolates (Piru isolate, Kairatu isolate and Uraur isolate) had faster inhibition against Fusarium oxyforum f. sp. cubense causes wilt disease on banana kepok (Musa paradisiaca normalis) in vitro with antagonism test. The research was conducted in 2 places, namely in the HPT BPTPH laboratory in Maluku province and continued in the microbiology laboratory at FMIPA Unpatti. The research method used was laboratory experimental in nature by using a completely randomized design with factorial pattern with three treatments and three replications. The results showed that the cause of wilt disease in banana plants was Fusarium oxyforum f. sp. Cubense. Antagonism test in vitro showed that T. harzianum Piru isolate showed the highest inhibition zone (70.87%), both Kairatu isolates (45.27%), and the lowest by Uraur isolate (32.61%) so that it can be said that T. harzianum has the potential to inhibit the growth of Fusarium fungus. oxyforum f. sp. Cubense.
Co-Authors Adrien Jems Akiles Unitly Amos Killay Febby J. Polnaya Hermanus Nawaly Kusadhiani, Indrawanti La Eddy Lapu, Petrus Loppies, Cindy Loppies, Cindy R M Loppies, Cindy RM, Lysay, Claudia Priscia Mainassy, Meilissa C Martha Kaihena Pattikawa, Apriyanti Santi Patty, Jogeneis Pieter Kakisina Ratulewen, Desi Ellida Ruslan Husen Saban Tawari Sanita Suriani Savitri, Imelda K E Setha, Beni Seumahu, Cecelia Anna Seumahu, Cecillia Anna Siahaya , Anho Netty Siahaya, Anho Netty Siahaya, Antho N Tahitu, Ritha Tuhumuri, Evelin Ukratalo, Abdul M