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All Journal Jurnal Akademika Biologi Islamic Management: Jurnal Manajemen Pendidikan Islam Jurnal Ilmiah Keperawatan Social, Humanities, and Educational Studies (SHEs): Conference Series Lisan An Nathiq : Jurnal Bahasa dan Pendidikan Bahasa Arab Aphorisme: Journal of Arabic Language, Literature, and Education EKONOMIKA45 Language-Edu Jurnal Kajian Dan Penalaran Ilmu Manajemen Khirani: Jurnal Pendidikan Anak Usia Dini Jurnal Ekonomi Manajemen Dan Bisnis (JEMB)
Siti Nur Jannah
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Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Nursing Public Health Social Sciences Veterinary

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KOMUNITAS RHIZOBAKTERIA TANAMAN TEH DENGAN APLIKASI FORMULA BIOIMUNIZER (Chryseobacterium Sp DAN Bacillus Sp) BERDASARKAN GEN 16S rRNA Agnistisya Widaranti; Siti Nur Jannah; Hermin Pancasakti Kusumaningrum; Dwi Ningsih Susilowati
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Commodity of tea (Camellia sinensis) has an important role in the national economy, especially in the field of agro-industries. Based on data obtained from the Directorate General of Plantation, the tea plant productivity declined over the last few years. This is due to the pest attack which result in decreasing the productivity of the tea plant. Increased crop productivity of tea have been done, such as the use of herbicides and insecticides, but until now there is very little effort to increase the production of tea plants by the use of biological agents. Chryseobacterium sp and Bacillus sp are rhizobacteria in tea rhizosphere that could potentially be used as a biocontrol agent (bioimmunizer). The purpose of this study is to determine the community rhizobacteria in tea soil with the addition of bioimmunizer based on 16S rRNA gene using T-RFLP technique. The method used in this research is T-RFLP technique (Terminal Restriction Fragment Length Polymorphism) using enzymes Msp I and Rsa I. The calculated value are relative abundance, Shannon diversity index (H '), evenness index (E), and dominance index. The results of this study indicate that soil samples with the addition of bioimunizer consisting of Arthrobacter sp, Bacillus sp, Actinobacteria, and Chryseobacterium sp.Keyword: T-RFLP, Chryseobacterium sp, Bacillus sp
ISOLASI BAKTERIOFAG Salmonella spp. dari BIOFILM pada SISTEM AIR MINUM ISI ULANG Rahayu Damayanti; Siti Nur Jannah; w wijanarka; Sri Hartin Rahaju
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The public demands for the refill drinking water increases causing the development of refill drinking water industries. However, the problems occurred is no standard method to process drinking water with sterile and lack of government oversight. These cases give rise to sanitation which is the formation of bacteria pathogen forming biofilms in refill drinking water system. One of the bacteria pathogens is Salmonella. Salmonella in refill drinking water can cause diarrhea, because it can produce cytotoxin and enterotoxin. Bacteriophages are viruses that infect bacteria. This study aims to find natural isolates of bacteriophage from biofilm samples to infect Salmonella spp. in refill drinking water system. The isolates obtained is then characterized by biochemical test including Gram stain, a test Kligler Iron Agar (KIA) and api assay 20 E. The positive Salmonella spp. isolates are in the second dilution refill drinking water depot. The isolation of bacteriophage from biofilm is conducted with bacteriophages amplification and bacteriophage filtrate. The Infection test is performed by using Salmonella enterica, Salmonella 7A1 from Teluk Ambon and Salmonella spp. from refill drinking water depot. Platting is performed on serial dilutions of 10-2 to 10-10phage dilution. Positive result is characterized by the formation of plaque which is in source water samples, water product and drinking water depot. The number of plaques formed is calculated by Plaque Forming Units (PFU/mL) to determine quantification or calculation phages.
KERAGAMAN SECARA MOLEKULER BAKTERI ASAM LAKTAT PADA ILEUM DAN SEKUM AYAM BROILER YANG DIBERI PERLAKUAN PAKAN HASIL FERMENTASI Chrysonilia crassa Husnul Khotimah; Siti Nur Jannah; Rejeki Siti Ferniah
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Lactic Acid Bacteria (LAB) are microflora in the digestive tract that has positive roles in poultry’s health. The amount and diversity of LAB in the gastrointestinal tract are influenced by several factors, one of them is by the feeding factor. The study aims to analyze the LAB diversity in ileum and cecum after feeding of fermented Chrysonilia crassa molecularly. Analysis of LAB spesies diversity is needed to provide a baseline profile of microbial community database on the illeum and cecum digestive tract of broiler chicken between control (commercial feed) and treatment (feed by Chrysonilia crassa fermentation) by the method of Terminal Restriction Fragment Length Polymorphism (T-RFLP). The calculated values are the number of phyilotypes, relative abundance, Shannon-Wiener (H') diversity index, evenness (E), and similarity. Group of LAB detected in the control were Lactobacillus delbrueckii (180 bp), Lactobacillus sp. (187 bp), Lactobacillus plantarum (572 bp), uncultured bacterium (87 bp) and unidentified (50 bp, 582 bp). The result of this study showed that by giving the fermented feed by Chrysonilia crassa had resulted in the decreasing of LAB diversity in ileum (0.66), cecum (0.48) compared with commercial feed (control) in ileum (0.84), cecum (1.05). Keywords: broiler chicken, cecum, ileum, LAB, T-RFLP
DETEKSI GEN tlh DAN tdh PADA Bakteri Vibrio parahaemolyticus DARI AIR TAMBAK UDANG VANNAME (Litopenaeus vannamei) DI KABUPATEN REMBANG Adila Nawan Hasrimi; Anto Budiharjo; Siti Nur Jannah
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Vibrio parahaemolyticus is hallophilic gram-negative bacteria that live as natural inhabitant in aquatic environment. All Vibrio parahaemolyticus strain known to have thermolabile hemolysin encoded by tlh gene as species marker. Thermostable direct hemolysin encoded by tdh gene is responsible for regulating one of the virulence factors in Vibrio parhaemolyticus. The aim of this research is to detect tlh gene and tdh gene from water of vanname shrimp’s aquaculture in Rembang regency. Colonies of green-blueish bacteria grew from the isolation of  vanname shrimp’s aquaculture water in CD-VP media which is identified as Vibrio parahaemolyticus. The isolated bacteria is specifically identified as Vibrio parahaemolyticus bacteria by the detection of tlh gene. Molecular analysis shows tdh negative result that indicates tdh gene is not present in the isolated bacteria. Vibrio parahaemolyticus isolate were cultured in Wagatsuma agar for the tdh gene confirmation test that showed Kanagawa negative result, in which indicated that V. parahaemolyticus did not produce thermostable direct hemolysin. Vibrio parahaemolyticus isolate did not show any virulence factors to initiate host colonization in the aquatic environment. Keywords: Vibrio parahaemolyticus, tdh gene, tlh gene
KERAGAMAN BAKTERI ASAM LAKTAT SECARA MOLEKULER PADA ILEUM DAN SEKUM AYAM BROILER YANG DIBERI PAKAN PREBIOTIK BEKATUL DAN BEKATUL HASIL FERMENTASI Laelatul Baniyah; Siti Nur Jannah; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Lactic Acid Bacteria (LAB) is a digestive tract microflora that play a positive role in poultry health. The number and diversity of LAB in the digestive tract affected by several factors, among them was the kind of feed. The purpose of this research was  to know the molecular diversity of Lactic Acid Bacteria (LAB) in broiler’s ileum and cecum  after  feeding with  prebiotic bran and Rhizopus oryzae fermented  bran which was added to commercial feed. The molecular analysis was done using T-RFLP method , Hae III and Msp I were used as restriction enzymes.  The number of phylotype, relative abundance, Shannon diversity index (H '), evenness (E), and Dominance (D)  were examined . The results indicated that the addition of bran prebiotics on commercial feed showed a higher  diversity of lactic acid bacteria on broiler’s ileum and cecum, compared  with the addition of Rhizopus oryzae fermented  bran. The dominant BAL types are Lactobacillus spp, L. delbrueckii subs. Bulgaricus, L. intermedius, L. amilovorus, uncultured bacteria 87 bp, 280 bp, 331 bp and unidentified bacteria 74 bp, 82 bp, 131 bp. Keywords: Diversity, Lactic Acid Bacteria, Prebiotics, Bran, T-RFLP
IDENTIFIKASI JENIS PIGMEN DAN UJI POTENSI ANTIOKSIDAN EKSTRAK PIGMEN BAKTERI Rhodococcus sp HASIL ISOLASI DARI SEDIMEN SUMBER AIR PANAS GEDONG SONGO Muhamad Fikri Zulfikar; Endang Kusdiyantini; Siti Nur Jannah
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Pigments are the coloring agents commonly used in the pharmaceutical, cosmetic, and food industries. This compound has the ability as antibacterial, anticancer, antibiotic and antioxidant. Pigment can be obtained synthetic and natural, natural pigments can be produced by plants, animals and microbes, one of which microbes is Rhodococcus sp. This study was conduct to identify the pigment found in Rhodococcus sp and measure the antioxidant activity of pigment produced by Rhodococcus sp. Growth and pigments production Rhodococcus sp was  grown on NB (Nutrient broth) medium with 96 hours incubation. This Growth was measured by dry weight cell. Identification of pigment using Thin Layer Chromatography and spectrophotometer UV-Vis. Measured of antioxidant activity Rhodococcus sp pigment using Carotene Bleaching methods. The results of pigments obtained from the identification by separation of pigment samples by thin layer chromatography resulted in 2 fractions on silica gel plate GF 250 with Rf values of 0.75 and 0.90. and analysis of UV-Vis spectrophotometers obtained optimum wavelengths 395 and 420 nm is beta-carotene pigments. Rhodococcus sp pigment measured its antioxidant activity with carotene bleaching method with the antioxidant activity value 51,24%.Keywords: Pigment, Rhodococcus sp, Pigment identification, antioxidant activity
DETEKSI MRSA (Methicillin Resistant Staphylococcus aureus) PADA PASIEN RUMAH SAKIT DENGAN METODE MALDI-TOF MS DAN MULTIPLEX PCR Dian Wahyu Kemalaputri; Siti Nur Jannah; Anto Budiharjo
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Methicillin Resistant Staplylococcus aureus (MRSA) is a strain of Staphylococcus aureus which has been resistant to methicillin antibiotics and β-lactam group. The mechanism of MRSA resistance occurs because the Staphylococcus aureus produces Penicillin Binding Protein (PBP2a) encoded by the mecA gene that has low affinity to methicillin. Nowadays, MRSA is tested by resistance test using Oxacillin 1 ug. This method requires pure isolate and bacterial culture, so that the results can be seen in 5 days. To find a rapid and precise diagnostic technique for detecting MRSA, mecA gene detection utilizing PCR multiplex technique as the alternative diagnostics. This study aims to find a quick and precise alternative diagnostic technique for MRSA examination, which is utilizing MALDI-TOF MS and multiplex PCR technique.Keywords: MRSA, Staplylococcus aureus, MALDI-TOF MS, Multiplex PCR.
ISOLASI BAKTERIOFAG SPESIFIK Pseudomonas sp. DA1 DARI BIOFILM PADA SISTEM PENGISIAN AIR MINUM ISI ULANG. Zikra Hayati; Siti Nur Jannah; Agung Suprihadi
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The social demand for drinking water increases both in urban and rural areas. The state encourages the development of Industrial Water Supply Depot (DAM) refills. problems occurred is no standard method for treating drinking water is sterileand lack of government supervision. This raises the issue of sanitation that is the presence of pathogenic bacteria that form biofilms in drinking water refill system. One of the bacterial pathogen is Pseudomonas. Bacteria Pseudomonas at refill drinking water can cause infections in the digestive tract. This study aims to find specific bacteriophage isolates of biofilm samples to infect bacteria Pseudomonas sp. on refill drinking water system. Isolation host Pseudomonas done in 2 ways dilution and filtration and grown on selective media Pseudomonas Isolation Agar. Isolates obtained were then characterized by gram staining and Kligler Iron Agar (KIA) test. Isolation of bacteriophages of biofilm done by bacteriophage amplification and filtration to obtain filtrate bacteriophage I and II. Test performed by using the host's infection Pseudomonas sp. DA1, Pseudomonas aeruginosa and Salmonella sp. (LIPI’s collection). The results showed that the drinking water refill depot contained positive isolates of Pseudomonas is Pseudomonas sp. DA1 and obtained isolate specific bakteriofag that can infect Pseudomonas sp. DA1. Bacteriophage titer calculation results on each sample at 3,0 x 105 PFU/ml in drinking water depot, 3,3 x 107 PFU/ml in the water product, and 9 x 107 PFU/ml water sources. Keyword: refill drinking water systems, biofilms, Pseudomonas sp DA1, bacteriophage
KOMUNITAS BAKTERI RHIZOSFER TEH MELALUI APLIKASI BIOIMUNIZER (Chryseobacterium sp. dan Alcaligenes sp.) DENGAN METODE TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM (T-RFLP) Elin Savitri Aviani; Dwi Ningsih Susilowati; Siti Nur Jannah; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Types of bacteria from the rhizosphere communities actually have been developed as bioimunizer but their consistency is still need further experiment about indigenous bacteria associated with rhizosphere of plants. This study aims to identification on the tea plant rhizosphere bacterial communities which in previous studies has been infected with E. vexans Massee and given bioimunizer as well as the checking existence Chryseobacterium sp. and Alcaligenes sp. The result research showed that communities of bacteria on control samples obtained through culturing Bacillus sp. (51.91%), Acidobacteria bacterium (39.42%) and Actinobacteria sp. (8.66%). Control sample through metagenom obtained Gemmatimonas aurantiaca (5.80%), Bacillus sp. (42.55%), Acidobacteria bacterium (23.45%) and Actinobacteria sp. (28.20%). Communities of bacteria in the samples treated by culturing obtained Gemmatimonas aurantiaca (3.58%), Bacillus sp. (30.76%), Pseudomonas sp. (5.55%) Acidobacteria bacterium (13.94%) and Actinobacteria sp. (46.16%). Communities of bacteria in the samples treated by metagenom found Bacillus sp. (10.66%), Acidobacteria bacterium (4.22%), Actinobacteria sp. (5.48%), Uncultured bacterium (1.49%), Alcaligenes sp. (36.95%) and Chryseobacterium sp. (46.82%). The existence of Alcaligenes sp. and Chryseobacterium sp. show consistency bioimunizer composition is applied. Communities of bacteria on metagenom have diversity and evenness level higher than the culturing approach. Keywords: bacterial communities, T-RFLP, Chryseobacterium sp., Alcaligenes sp.
ISOLASI, KARAKTERISASI Aeromonas hydrophila DAN DETEKSI GEN PENYEBAB PENYAKIT MOTILE AEROMONAS SEPTICEMIA (MAS) DENGAN 16S rRNA DAN AEROLYSIN PADA IKAN LELE (Clarias sp.) M Muslikha; Sri Pujiyanto; Siti Nur Jannah; Hessy Novita
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Aeromonas hydrophila merupakan bakteri Gram negatif yang banyak ditemukan di perairan dan dapat menyerang ikan. Selain itu, A. hydrophila menyebaban penyakit Motile Aeromonas Septiemia (MAS) yang menyerang beberapa organ dalam seperti hati, limpa dan ginjal. Isolat bakteri diisolasi dari ikan lele (Clarias sp.) yang berasal dari berbagai daerah seperti, Ciganjur, Sukamandi dan Citayam. Penelitian ini bertujuan untuk mengisolasi, karakterisasi dan deteksi gen patogen bakteri A. hydrophila penyebab penyakit MAS pada ikan lele. Beberapa pengujian yang dilakukan pada penelitian ini yaitu uji biokimia (pewarnaan Gram, uji oksidatif-fermentatif, uji katalase, uji oksidase, uji d-mannitol, uji TSA skim milk, uji Mac Conkey, dan uji novobiosin), uji deteksi gen patogen dilakukan secara molekuler dengan menggunakan primer 16S rRNA dan aerolysin. Berdasarkan hasil pengujian biokimia, A. hydrophila merupakan Gram negatif dengan sel berbentuk basil pendek, bersifat motil, positif menghasilkan enzim oksidase, enzim katalase dan positif oksidatif dan fermentatif, positif fermentasi laktosa. Hasil deteksi gen patogen menunjukkan isolat AH2 dan B9 memiliki gen faktor virulen yaitu, aerolysin. Isolat AH2 dan B9 menghasilkan gen aerolysin kembali pada deteksi gen hasil reisolasi dari postulat Koch. Kata kunci : Ikan Lele (Clarias sp.), Aeromonas hydrophila, Gen Faktor Virulen, Motile Aeromonas Septicemia (MAS).
Co-Authors Abdul Aziz Adila Nawan Hasrimi Agnistisya Widaranti Agung Suprihadi Aisa, Aufia - Anto Budiharjo Cindi Octavia Dian Wahyu Kemalaputri Djauharotun Nafisah Dwi Indarti DWI NINGSIH SUSILOWATI Elin Savitri Aviani Endang Kusdiyantini Hermin Pancasakti Kusumaningrum Hessy Novita Hilda Zahra Lubis HUSNUL KHOTIMAH Khalida Ziah Sukma Siregar Laelatul Baniyah Laksono Trisnantoro M Muslikha Merina Widyastuti MG Isworo Rukmi Muhamad Fikri Zulfikar Ninik Ambar Sari Nur Hidayatun Nuzul Purnama Pramono Pramono Putri Silvia Rahayu Damayanti Rahma Zhafirah Rejeki Siti Ferniah Renny Oktafia Rina Dian Rahmawati Sapto Dwi Anggoro, Sapto Dwi Siti Musannadah Sri Anik Rustini Sri Hartin Rahaju Sri Pujiyanto SUTIKNO Vivi Dwi Yanti w wijanarka Zainuri