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All Journal Medical Laboratory Technology Journal Jaringan Laboratorium Medis An-Nadaa: Jurnal Kesehatan Masyarakat Tropical Health and Medical Research Journal Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Karya Generasi Sehat
Insana, Aima
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Oral Candida spp. Density as a Blood Glucose Marker in Diabetes Mellitus Patients Insana, Aima; Nurlailah, Nurlailah; Adawiyah, Rabiatul
Tropical Health and Medical Research Vol. 7 No. 2 (2025): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v7i2.133

Abstract

Candida spp. is a commensal fungus in the oral cavity that can become an opportunistic pathogen when there is a decrease in the immune system or metabolic disorders such as diabetes mellitus. Hyperglycemia in people with diabetes increases salivary glucose levels, which can be a nutrient source for Candida, thus triggering increased density, biofilm formation, and antifungal resistance. This study aims to analyse the relationship between blood glucose levels and oral Candida spp. density in patients with diabetes mellitus. The study used a cross-sectional design in 29 Guntung Manggis Banjarbaru Community Health Centre patients. Saliva samples were collected by mouthwash and planted on Hichrome Candida Differential Agar (HCDA) media to calculate colony density. At the same time, blood glucose levels were measured spectrophotometrically using the GOD-PAP method. The results showed abnormal blood glucose levels (?200 mg/dL) were found in 16 respondents (55.2%), and abnormal Candida spp. density (>500 CFU/mL) was also found in 16 respondents (55.2%). The growth of Candida spp. detected in 24 samples (82.8%). The Chi-square test showed a significant association between hyperglycemia and increased oral Candida spp. density (p = 0.017). These findings suggest that hyperglycemia plays a role in increased Candida density in the oral cavity. Hence, monitoring blood glucose levels and oral Candida density is important for preventing fungal infection complications in patients with diabetes mellitus.
Comparison of TCM GeneXpert MTB/RIF Ultra Examination Results with AFB Microscopic Examination in Pulmonary TB Patients with MTB Detected Ulfah, Putriana; Dwiyanti, Ratih Dewi; Insana, Aima
Tropical Health and Medical Research Vol. 7 No. 2 (2025): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v7i2.135

Abstract

Tuberculosis (TB) is a disease in Indonesia with the second largest number of cases in the world after India. One of the TB-free strategies is early diagnosis with bacteriological laboratory examination for rapid treatment, thereby reducing the incidence rate. WHO recommends the GeneXpert molecular rapid test for confirming the diagnosis of TB. The TB diagnosis is confirmed by Acid-Fast Bacilli (AFB) microscopic examination for laboratories with difficulty accessing rapid molecular tests. The study aims to compare the rapid molecular test GeneXpert MTB/RIF Ultra examination with Ziehl-Neelsen stained AFB microscopic examination in diagnosing lung patients who have been detected with Mycobacterium tuberculosis very low, low, medium, and High (MTB detected)—the type of observational analytical study, with a cross-sectional design to understand the differences. The sampling technique is a total sampling of 30 samples, and data analysis using the Wilcoxon statistical test. Of the 30 samples tested for Mycobacterium tuberculosis (MTB), the Molecular Rapid Test identified all samples as positive (100%). In contrast, AFB microscopy detected only 23 positive cases (76.67%) and failed to detect MTB in 7 samples (23.33%). Based on bacterial load classification, three samples (10%) were categorized as very low, with AFB results showing two negatives (6.67%) and one scanty (3.33%). Among the nine low-load samples (30%), five were AFB-negative (16.67%) and four were positive (1+) (13.33%). In the seven medium-load samples (23.33%), microscopy detected five samples as 1+ (16.67%), two as 2+ (6.67%), and one as 3+ (3.33%). Of the 11 high-load samples (36.67%), AFB identified three as 2+ (10%) and eight as 3+ (23.33%). The conclusion of the results of the Molecular Rapid Test examination with AFB microscopic there shows a significant difference (P < 0.025) < ? (0.05). It is recommended that further research be conducted to compare three bacteriological examinations, namely rapid molecular test, AFB microscopic, and culture, as a gold standard examination.
Family Socioeconomic Factors as a Risk Factor for ARI Incidence in Toddlers in Pekauman Dalam Village, Banjar Regency, Indonesia Dwiyanti, Ratih Dewi; Insana, Aima
Tropical Health and Medical Research Vol. 7 No. 2 (2025): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v7i2.138

Abstract

Acute Respiratory Tract Infections (ARI) remain a leading cause of infant mortality worldwide, including in Indonesia. Banjar Regency, South Kalimantan, has the highest infant pneumonia mortality rate in the province, despite its lower prevalence. This situation necessitates a study of the risk factors contributing to the incidence of ARI, particularly from a family's socioeconomic perspective. This study aims to determine the relationship between the incidence of ARI in toddlers and family socioeconomic risk factors in Pekauman Dalam Village, Banjar Regency. This study used an analytical study design with a cross-sectional approach. A sample of 28 toddlers was selected using total sampling. Data were collected through structured questionnaires completed by the toddlers' mothers/caregivers. Data analysis was performed bivariately using simple binary logistic regression, with further analysis using the Chi-Square test and Fisher's Exact Test on significant variables. The results of the study showed that among the 28 toddlers examined, six toddlers (21.4%) exhibited symptoms of acute respiratory infections (ARI). Statistical analysis revealed that, among all variables tested, only the toddler's age had a significant association with ARI incidence (p = 0.001; Fisher's Exact Test = 0.011). Toddlers aged ?1 year had a higher proportion of ARI cases than toddlers aged >1 year. Other variables such as family income, home ownership, residential density, cooking fuel, and maternal education did not show statistically significant relationships, although some had p-values approaching the significance threshold. Toddler age was the main risk factor influencing ARI cases in this study. Although most other socioeconomic factors were not statistically significant, these aspects are still important to consider in ARI prevention efforts. This study can form the basis for more targeted preventive interventions in areas with high socioeconomic vulnerability.
The Effect of Sputum Delivery Time on the Sensitivity of Culture and AFB Microscopy in the Diagnosis of Drug-Resistant TB Rizkiyah, Risa; Dwiyanti, Ratih Dewi; Insana, Aima
Tropical Health and Medical Research Vol. 7 No. 2 (2025): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v7i2.139

Abstract

Drug-resistant tuberculosis (DR-TB) is a major challenge in TB control in Indonesia. Early detection using liquid and solid media cultures is crucial due to the limited sensitivity of Acid-Fast Bacilli (AFB) microscopic examination. In addition, the time of sputum specimen delivery can also affect bacterial viability. This study aims to determine the relationship between liquid and solid media culture results with AFB microscopic results in immediate and delayed sputum in patients with DR-TB. This is an observational analytical study with a cross-sectional design, involving 40 sputum samples from DR-TB patients examined at the South Kalimantan Provincial Health Laboratory from January to April 2025. Samples were grouped based on the time of sputum delivery (<7 days = immediate; ?7 days = delayed), and examined using AFB microscopic methods, Mycobacterium Growth Indicator Tube liquid culture, and Lowenstein-Jensen solid culture. Data analysis was performed using the Spearman correlation test. Of the 40 sputum samples examined, 2 (5%) were positive by AFB microscopy, 8 (20%) were positive by liquid culture (MGIT), and 6 (15%) were positive by solid culture (Lowenstein-Jensen). The results showed that in immediate sputum, Mycobacterium tuberculosis (MTB) was detected in 7 samples using liquid media and in 5 samples using solid media. In contrast, in delayed sputum, only one sample was positive in both media. Most samples with negative AFB microscopy still yielded MTB growth in culture, particularly in immediate sputum. There was no significant correlation between AFB and liquid culture results in immediate sputum (p=0.172), but there was a substantial correlation between AFB and solid culture (p=0.025). In delayed sputum, both liquid and solid cultures showed a significant correlation with AFB results (p=0.046). Liquid culture was proven to be more sensitive than AFB microscopic examination, especially for immediately examined sputum. MTB viability and accuracy of results decreased in late-delivered sputum, so optimal specimen delivery management is necessary to maintain diagnostic quality.
Pengaruh Lama Penyimpanan Jamu Beras Kencur terhadap Pertumbuhan Kapang Pinto Laksono, Fransiskus Xaverius Agung; Dwiyanti, Ratih Dewi; Rifqoh, Rifqoh; Insana, Aima
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8571

Abstract

During the Covid-19 pandemic, people began to maintain their health, by using herbal medicine as an alternative treatment. One type of herbal medicine used is jamu beras kencur because it has properties to increase stamina, increase appetite, and relieve aches and cough disorders. In the Regulation of the Minister of Health of the Republic of Indonesia No. 007 of 2012 carrying herbs such as jamu beras kencur does not require marketing authorization. The quality of the herbal medicine produced has not been confirmed as feasible from microbial contamination, especially in molds, the number of yeast molds in the jamu beras kencur must comply with BPOM standards Number 32 of 2019 namely AKK <103 colonies/mL. This study aimed to determine the effect of the storage time of jamu beras kencur on mold growth with storage treatments of 0 hours, 4 hours, 8 hours, 12 hours, and 24 hours at room temperature. This type of research is a Quasi Experiment with a research design of One Group Pretest-Postest Design. The results of this study showed that the average AKK value was 0 hours 0.2x100 colonies/mL, 4 hours 0.4x100 colonies/mL, 8 hours 0.4x100 colonies/mL. 12 hours 0.8x100 colonies/mL and 24 hours 2.2x100 colonies/mL. These results stated that the jamu beras kencur met the BPOM standard requirements Number 32 of 2019 which was AKK <103 Colonies/mL. The results of the Kruskal Wallis test obtained a sig value of 0.286, it was concluded that there was no effect of storage time for the jamu beras kencur on the growth of molds. The identification results on the jamu beras kencur obtained fungal species, namely Aspergillus flavus, Aspergillus niger, and Penicillium sp. Suggestions for further researchers can examine the effect of storage time on mold growth in other types of herbal medicine.
Salıva pH, Carıes and OHI-S Score in Stuntıng Pre-School Chıldren in The Swamp Area Utami, Naning Kisworo; Andrestian, Meilla Dwi; Insana, Aima
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.688

Abstract

Stunting remains a major global health issue and is associated with impaired physical and cognitive development, as well as increased susceptibility to oral diseases such as dental caries. Environmental factors, including acidic peatland water, may further aggravate oral health conditions among children living in swamp areas. This study aimed to analyze the relationship between salivary pH, dental caries severity (def-t index), and oral hygiene status (OHI-S) in preschool children with stunting living in peatland regions of South Kalimantan, Indonesia. An analytical observational study with a cross-sectional design was conducted involving 32 preschool children aged 2–5 years residing in the working area of the Martapura Timur Public Health Center. Data collection included clinical examination of caries using the WHO criteria, salivary pH measurement with a Krisbow digital pH meter, and assessment of oral hygiene using the Simplified Oral Hygiene Index (OHI-S). Statistical analysis was performed using the Spearman correlation test. The results showed that salivary pH ranged from 5.5 to 7.0, def-t scores ranged from 2 to 8 (mean = 5.34 ± 1.78), and OHI-S scores were 0.00 in 22 children (68.7%) and >0.00 in 10 children (31.3%). A significant association was found between salivary pH and dental caries severity (p = 0.005; α = 0.05), indicating that lower salivary pH is associated with higher caries prevalence. In conclusion, stunted preschool children in peatland areas exhibited acidic salivary conditions and a high caries burden despite relatively good oral hygiene status. These findings highlight the importance of integrating nutritional rehabilitation and oral health education programs to reduce the risk of dental caries in children living in environmentally vulnerable swamp regions.
Antithrombotic Activity and Hemolysis Pattern of Fibrinolytic Protease-Producing Bacterial Isolates from the Coast of Tanjung Dewa, South Kalimantan Lutpiatina, Leka; Dwiyanti, Ratih Dewi; Insana, Aima; Rizal, Muhammad; Mufid, Muhammad
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.689

Abstract

Cardiovascular disease is a leading cause of death worldwide, with thrombosis playing a key role in the pathological process. Conventional antithrombotic therapy often causes side effects, necessitating alternatives based on biological resources. Marine microorganisms are known to produce fibrinolytic protease enzymes that can degrade fibrin and prevent blood clot formation. This study aimed to evaluate the differences in antithrombotic activity and hemolysis patterns of fibrinolytic protease-producing bacterial isolates obtained from the coast of Tanjung Dewa Beach, South Kalimantan. A total of 15 isolates were obtained from seawater, beach sand, mollusks, crabs, and barnacles, and then subjected to colony morphology identification, Gram staining, and spore observation. Proteolytic activity testing using Skim Milk Agar media showed that all isolates had proteolytic activity with varying indices, with PSR1 showing the highest index (3.4). Furthermore, fibrinolytic testing using the fibrin plate assay method showed that 10 isolates were capable of degrading fibrin, with AL8 and SP2 showing the highest fibrinolytic indices (3.12 and 3.11, respectively). Antithrombotic testing using the clot lysis method revealed that AL7 and SP1 exhibited the highest lysis percentages (82.05% and 88.88%, respectively). Anticoagulant activity, as determined by the Lee-White method, showed that SP2 significantly prolonged the coagulation time (142 seconds, 49.65%). Hemolysis pattern testing revealed variations in activity, with AL8 and PSR1 classified as gamma-hemolytic and therefore potentially safer. Statistical analysis showed no significant differences between isolates in terms of antithrombotic activity, anticoagulant activity, or hemolysis pattern (p > 0.05). These findings suggest that coastal bacterial isolates from Tanjung Dewa Beach, particularly AL8 and SP2, have the potential to serve as safe, natural antithrombotic agents, supporting the development of biomedical therapies for cardiovascular disease.
Co-Authors Ahmad Muhlisin Dewi Dwiyanti, Ratih Erfan Roebiakto, Erfan Habibah, Siti Noor Hafidh, Muhammad Ipniliati, Eva Khairir Rizani, Khairir leka lutpiatina, leka Meilla Dwi Andrestian, Meilla Dwi Mufid, Muhammad MUHAMMAD RIZAL Nur Ain Mirandayanti Nurlailah Nurlailah, Nurlailah Pinto Laksono, Fransiskus Xaverius Agung Putri Wulandari, Rima Putri, Noorlaily Rabiatul Adawiyah Ratih Dewi Dwiyanti, Ratih Dewi Rifqoh Rifqoh Rifqoh, Rifqoh Rizkiyah, Risa SATRIYAS ILYAS Ulfah, Putriana Utami, Naning Kisworo Wahdah Norsiah, Wahdah Wulandari, Eka Dwi