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All Journal Media Penelitian dan Pengembangan Kesehatan Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF PHARMACY Gadjah Mada Journal of Psychology Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Ahmad Husain Asdie
Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Ability of Curcuminoid from Curcuma domestica Val. in Reducing the Secretion of Reactive Oxygen Intermediates by Synovial Fluid Monocytes in Patients with Osteoarthritis Kertia, Nyoman; Asdie, Ahmad Husain; Rochmah, Wasilah; -, Marsetyawan
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

AbstractIncreasing the secretion of reactive oxygen intermediates (ROI) by monocytes in the synovial fluid is anindicator to determine the severity of joint inflammation. Previous studies have shown that curcumin inhibitthe osteoarthritis progression with its ability to inhibite the activity of the nitric oxide synthase (NOS) enzymefrom macrophages. In this prospective randomized open end blinded evaluations = PROBE study, 80 patientswith knee osteoarthritis were eligable. The subject were devided in to two group: group who received 3 x 30mg of curcuminoid from Curcuma domestica Val. extract (curcuminoid group) and group who received 3 x 25mg of diclofenac sodium (diclofenac group) as comparison. The treatment was for 4 weeks time. The secretionof ROI by sinovial fluid monocytes was calculated by scoring the amount of formazan formation after neutralred staining in nitrobleu tetrazolium reduction assay. The result of this study showed that the secretion of ROIby synovial fluid monocytes was significantly decreased in both groups (p <0.001) respectively. There wasno significant difference in decreasing of ROI secretion of synovial fluid monocytes between both treatmentgroups (p = 0.92).Keywords : curcuminoid, diclofenac sodium, reactive oxygen intermediates, monocyte, osteoarthritis
Genetic Variation of Apolipoprotein E (ApoE) in Surabaya, Palu and Alor Populations of Indonesia Hastuti, Pramudji; Sofro, Abdul Salam Mudzakir; Asdie, Ahmad Husain; Sadewa, Ahmad Hamim
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

AbstractApolipoprotein E (ApoE) has been considered to play an important role in cardiovascular disorders.Several studies reported that genetic variation in ApoE locus influence plasma lipoprotein level. The objectivesof this study was to compare the frequency of ApoE genotypes and alleles in some populations of Indonesia.One hundred and ninety five voluntarily unrelated apparently healthy individuals were recruited fromSurabaya, Palu and Alor representing the western, middle and eastern populations of Indonesia, respectively.Blood samples were collected from each subject for DNA extraction. The common allelic variants of ApoE werescreened using polymerase chain reaction (PCR) and restriction fragment length polymorphism. Three allelesi.e. ε2, ε3 and ε4 were identified and five genotypes i.e. ApoE ε2/ε2, ApoE ε2/ε3, ApoE ε3/ε3, ApoE ε2/ε4, ApoE ε3/ε4 were found in three populations studied, while ApoE ε4/ε4 was absent in Surabaya, representing the westernpopulations of Indonesia. The frequency of ε2, ε3 and ε4 alleles in the western population were 0.208, 0.701and 0.092 respectively; in the middle population were 0.242, 0.618 and 0.140 respectively and in the easternpopulation of Indonesia were 0.267, 0.466 and 0.267 respectively. The highest frequency of ε2 and ε4 allelewas found in the eastern population of Indonesia. The distribution of ε2 allele were not significantly differentamong all Indonesian populations, but significantly different were found in ε3 and ε4 allele in the easternpopulation compared to those in the western and middle populations of Indonesian. It can be concluded thatthe frequency of three ApoE alleles in the western and middle populations of Indonesia was not significantlydifferent however, significantly different was observed in the frequency of ApoE ε3 and ε4 alleles from theeastern compared to those in the western and middle populations of Indonesia.Keywords : Apolipoprotein E; genotypes; allele frequency; populations of Indonesia
Apolipoprotein E as Risk Factor for Coronary Heart Disease Hastuti, Pramudji; M Sofro, Abdul Salam; Asdie, Ahmad Husain; Sadewa, Ahmad Hamim
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Allelic variation of apolipoprotein E (apo E) has been shown to infl uence the concentrations of total cholesterol and low density lipoprotein cholesterol (LDL-C) and considered to play a role as one of risk factors for coronary heart disease (CHD). The aim of this study was to examine the relationship between Apo E polymorphism and the risk of CHD. Blood samples were collected from 33 CHD patients in Dr. Sardjito Hospital Yogyakarta, and 38 apparently healthy control individuals in a cross sectional study. The common allelic variants of ApoE were screened employing polymerase chain reaction and restriction fragment length polymorphism. The results obtained were analyzed by t-test and signifi cantly different if p <0.05 and risk factor was calculated by odd ratio. Frequency of ApoE ε2, ε2 and ε4 alleles in CHD patients were 12.1%, 69.7% and 18.2% while in controls were 18.4%, 72.4% and 9.2% respectively. Dyslipidemia condition was a strongrisk factor for CHD. By controlling lipid profi le and applying multifactorial statistic analysis, it was shown that ε4 gene carrier was the risk factor for CHD, but not in triglyceride level, whereas ε2 carrier gene was not the risk factor for CHD. Dislipidemia was the risk factor for CHD and ApoE ε4 gene carrier was the risk factor for CHD.Key words: apolipoprotein E, ApoE ε4 gene carrier, coronary heart disease, dyslipidemia.
Effect of the combination of C.domestica extract and essential oil of C.xanthorrhiza roxb. on leucocyte count in synovial fluid compared to that of pyroxicam Kertia, Nyoman; A.D., Imono; ., Mufrod; Catur, Eny; Rahardjo, Poerwono; Asdie, Ahmad Husain
Indonesian Journal of Pharmacy Vol 16 No 3, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.783 KB) | DOI: 10.14499/indonesianjpharm0iss0pp155-161

Abstract

Osteoarthritis (OA) is the most prevalence rheumatic disease. The non steroidal antiinflamatory drugs display some side effect especially on the gastrointestinal tract, liver, kidney and blood clotting. Kunyit (Curcuma domestica L.) and temulawak (Curcuma xanthorrhiza Roxb.) as natural herbal remedies are developed for antiinflammation, antioxidant and cholagoga. The aim of this present research was to study the effect of combination of Curcuma domestica extract and essential oil of Curcuma xanthorrhiza Roxb on the leucocyte count in the synovial fluid of patient with osteoarthritis compared to that of pyroxicam. A double blind randomized clinical trial was used and conducted at Dr. Sardjito Hospital Yogyakarta. After washed out period the knee synovial fluid was aspirated and the leucocyte count was examined. Patients were randomized for receiving piroxicam capsule 10 mg twice daily or combination of 3,66 ± 0,65 % w/w curcuminoid extract of Curcuma domestica L.and 25 ml essential oil containing curcumen, bergamoten germacren B, Curserenon, Germacron, Xanthorrizol as a major component with 27,64 ± 0,85 % relative concentration of xanthorrhizol each capsule; two times a day for two week period. At the end of this study the synovial fluid was examined. Decreasing the inflamation of synovial area was parrallel with decreasing leucocyte count by 174.56 ± 161.73 per mm3 (p
PENGARUH TERAPI KURKUMINOID EKSTRAK RIMPANG KUNYIT DIBANDINGKAN DENGAN NATRIUM DIKLOFENAK TERHADAP FUNGSI GINJAL PENDERITA OSTEOARTRITIS Kertia, Nyoman; Asdie, Ahmad Husain; Rochmach, Wasilah; -, Marsetyawan
Media Penelitian dan Pengembangan Kesehatan Vol 21, No 4 Des (2011)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v21i4 Des.81.

Abstract

Background: Osteoarthritis is a joint disease most often found in the community. Patients with osteoarthritis are frequently drink non steroidal anti inflammatory drugs such diclofenac sodium. Data has shown too many side effects of diclofenac sodium especially to the kidney function. A research data in Malang shows that people who use herbs were 476 persons in city area and 580 persons in distric area per thousand patients with arthritis. Curcuma domestica Val. is one of Asia's native plants used to make herbal medicine mainly used to reduce inflammation. The purpose of this study was to assess the safety of curcuminoid from Curcuma domestica Val. rhizome extract compared to diclofenac sodium to the kidney function of patients with osteoarthritis.  Methods: This was a prospective randomized open and blinded evaluation (PROBE) study. Subjects with knee osteoarthritis were divided randomly into two groups: the group who received 30 mg 3 times daily of curcuminoid from Curcuma domestica Val rhizome extrac (curcuminoid group) and group who received 25 mg 3 times daily of diclofenac sodium (diclofenac group). Assessment of results including serum blood urea nitrogen (BUN) and creatinine level was performed before and after 4 weeks period of treatment. Data analysis compared the change of those levels in each group during the treatment period by student t test analysis. Results: In the curcuminoid group there was no significant decrease of serum BUN level (p=0.52) and there was a significant decrease of serum creatinine level (p=0.03). In the diclofenac group there was a significant increase of serum BUN level (p<0.01) and no significant increase of serum creatinine level (p=0.39). Increasing the serum level of BUN and creatinine in diclofenac group were significantly different compared to decreasing of those level in the curcuminoid group with p=0.01 and p=0.03 respectively. Conclusion: Treatment with Curcuminoid from Curcuma domestica Val. rhizome extract was significantly decreased the serum BUN and creatinin level compared to those increased level in diclofenac sodium treatment. AbstrakLatar belakang: Osteoartritis merupakan penyakit sendi yang paling banyak dijumpai di masyarakat. Pasien osteoartritis sering menggunakan obat anti inflamasi non steroid seperti natrium diklofenak. Data menunjukkan banyaknya efek samping natrium diklofenak khususnya terhadap fungsi ginjal. Data penelitian di Kotamadya dan Kabupaten Malang menunjukkan bahwa penduduk yang menggunakan jamu masing-masing adalah 476 orang dan 580 orang per seribu pasien. Kunyit (Curcuma domestica Val.) adalah salah satu tumbuhan asli Asia yang biasa dibuat jamu yang utamanya digunakan untuk mengurangi peradangan. Tujuan penelitian ini adalah untuk menilai keamanan terapi kurkuminoid ekstrak rimpang kunyit dibandingkan dengan natrium diklofenak terhadap fungsi ginjal penderita osteoartritis. Metode: Penelitian ini dirancang sebagai prospective randomized open and blinded evaluation (PROBE). Subjek dengan osteoartritis lutut yang memenuhi syarat dan bersedia ikut dalam penelitian ini dibagi menjadi dua kelompok secara random yaitu kelompok yang mendapatkan terapi kurkuminoid ekstrak rimpang kunyit 30 mg diminum 3 kali sehari (kelompok kurkuminoid) dan kelompok yang mendapatkan terapi natrium diklofenak 25 mg diminum 3 kali sehari (kelompok diklofenak). Penilaian hasil terapi yang meliputi kadar blood urea nitrogen (BUN) dan kreatinin serum dilakukan sebelum dan setelah 4 minggu terapi. Analisis data untuk membandingkan perubahan kadar BUN dan kreatinin pada masing-masing kelompok perlakuan dilakukan dengan student t test. Hasil: Pada kelompok yang mendapat terapi kurkuminoid ekstrak rimpang kunyit terjadi penurunan kadar BUN serum yang tidak bermakna (p=0,52) dan penurunan kadar kreatinin serum secara bermakna (p=0,03). Pada kelompok yang mendapat terapi natrium diklofenak terjadi peningkatan kadar BUN serum secara bermakna (p<0,01) dan peningkatan kadar kreatinin serum yang tidak bermakna (p=0,39). Peningkatan kadar BUN dan kreatinin serum pada terapi natrium diklofenak berbeda bermakna dengan penurunan kadarnya pada terapi kurkuminoid (p=0,01 dan p=0,03). Kesimpulan: Terapi kurkuminoid ekstrak rimpang kunyit menurunkan kadar BUN dan kreatinin serum penderita osteoartritis secara bermakna dibandingkan dengan peningkatan kadarnya pada terapi dengan natrium diklofenak.
Peran Faktor-faktor Psikologis terhadap Depresi pada Pasien Diabetes Mellitus Tipe 2 Donsu, Jenita DT; Hadjam, M Noor Rochman; Asdie, Ahmad Husain; Hidayat, Rahmat
Jurnal Psikologi Vol 41, No 2 (2014)
Publisher : Faculty of Psychology, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.087 KB) | DOI: 10.22146/jpsi.6953

Abstract

Depression has strong relationship with diabetes mellitus and the aspect of psychology has influence toward the diabetes patient as control. This research aimed to find out the role of psychological factors in dealing with depression suffered by patient of DM-2 and to see the dynamics of both direct and indirect relationship of psychological factors toward the depression of DM-2 patient. The main model is a model that can bring effect of mediation between social support and depression caused by DM-2. Optimism can provide mediating effect of the relationship between social support and self-esteem. Resilience can provide mediating effect of the relationship between social support and self-esteem. Similarly, self-esteem acts as the mediator of the relationship between optimism and resilience toward the depression of DM-2 patient. The result of estimation parameter test showed the direct relationship between social support toward optimism (0.717), resilience (0.811), self-esteem (0.269) and depression (-0.210) and the direct role to depression (-0.397). The higher social support and self-esteem will cause the higher optimism and resilience. On the other hand, the role of self-esteem showed negative coefficient. On the other hand, the role of self-esteem to depression showed a negative coefficient is, the higher self-esteem will be followed by the decrease of depression in patient with DM-2. Keywords: depression, optimism, resilience, self-esteem, social support, DM-2
Eksplorasi Kearifan Lokal Masyarakat dalam Mengonsumsi Pangan Sumber Zat Goitrogenik terhadap Gangguan Akibat Kekurangan Yodium Ningtyas, Farida Wahyu; Asdie, Ahmad Husain; Julia, Madarina; Prabandari, Yayi Suryo
Kesmas Vol. 8, No. 7
Publisher : UI Scholars Hub

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Abstract

Eksplorasi Kearifan Lokal Masyarakat dalam Mengonsumsi Pangan Sumber Zat GSelain kekurangan yodium, penyebab lain gangguan akibat kekurangan yodium (GAKY) di Kabupaten Jember adalah faktor goitrogenik tiosianat. Tiosianat adalah hasil detoksifikasi sianida. Sianida banyak terkandung pada beberapa sayuran yang biasa dikonsumsi. Tujuan penelitian ini adalah untuk mengeksplorasi kebiasaan konsumsi dan cara pengolahan pangan sumber zat goitrogenik sebagai solusi mengatasi GAKY di Kabupaten Jember. Dengan menggunakan pendekatan kualitatif, pengumpulan data dilakukan melalui focus grup discussion dilengkapi dengan semi-kuantitatif formulir frekuensi makan. Data yang terkumpul diolah dengan content analysis. Ada empat kelompok FGD yang diikuti 6 – 9 ibu rumah tangga yang terpilih melalui metode maximum variation sampling. Penelitian ini dilaksanakan pada bulan September 2012 – April 2013. Daun singkong, daun pepaya, rebung, sawi pahit, kubis dan selada air adalah sayuran sumber zat goitrogenik yang dikonsumsi harian dengan porsi yang cukup besar disebabkan faktor kesukaan dan kebiasaan oleh masyarakat Jember. Kadar sianida yang terkandung pada sayuran di kabupaten Jember berkisar 0,010 – 0,4 ppm dalam keadaan segar, tertinggi pada singkong dan terendah pada gambas dan kubis. Kadar sianidanya menjadi 0,18 – 0,0001 ppm setelah beberapa cara pengolahan yang biasa dilakukan oleh masyarakat Jember. Blansing/kulup adalah cara mereduksi kadar sianida yang paling baik dibandingkan cara pengolahan lain yang biasa dilakukan masyarakat Jember seperti rebus, tumis, goreng dan kukus. Other caused of iodine deficiency disorder (IDD) that was identified in Jember Regency is thiocyanate goitrogenic factor. Thiocyanate is the result of detoxification from cyanide content in some common vegetables consumed which consume daily. The purpose of this study was to explore goitrogenic food consumption habits and processing as a solution to overcome IDD in Jember Regency. Using a qualitative approach, data collection is done through focus group discussion equipped with a semi-quantitative food frequency form. The collected data were processed with content analysis. There are four groups of 6 – 9 FGD followed housewife selected through maximum variation sampling method. The study was carried out in September 2012 – April 2013. Cassava leaves, papaya leaves, bamboo shoot, cabbage, “sawi pahit” and “selada air/arnong” that vegetables contain substances goitrogenik consumed daily by a large enough portion due to factors fondness and familiarity. Cyanide content on vegetables from Jember district was around 0,01 – 0,40 ppm, the highest was in cassava and the lowest in cabbage and “gambas”. After some processing methods practiced by society, cyanide levels in foodstuffs become 0,18 – 0,0001 ppm. Blanching is the best way to reduce cyanide than the usual way as boiled, sauteed, fried and steamed.oitrogenik terhadap Gangguan Akibat Kekurangan Yodium
Perubahan Pola Konsumsi Pangan Sumber Zat Goitrogenik Sianida dan Cara Pengolahannya Melalui Penyuluhan Gizi Ningtyias, Farida Wahyu; Asdie, Ahmad Husain; Julia, Madarina; Prabandari, Yayi Suryo
Kesmas Vol. 9, No. 2
Publisher : UI Scholars Hub

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Abstract

Kabupaten Jember masih menghadapi masalah gizi gangguan akibat kekurangan iodium (GAKI), sebagian besar kecamatannya termasuk dalam kategori daerah endemik GAKI. Salah satu penyebabnya adalah faktor goitrogenik sianida yang mengganggu pembentukan hormon tiroid. Keberadaannya pada beberapa sayuran yang biasa dikonsumsi masyarakat menyebabkan diperlukannya pola konsumsi dan proses pengolahan yang baik agar aman dikonsumsi. Penelitian ini bertujuan mengubah pola konsumsi goitrogenik sianida dan cara pengolahannya melalui penyuluhan gizi dan demonstrasi cara pengolahan pangan sumber goitrogenik sianida yang benar. Penelitian ini adalah sebuah penelitian kuasi eksperimental dengan rancangan pretest-posttest control design. Jumlah sampel sebanyak 196 ibu rumah tangga, terdiri dari 98 orang di setiap kelompok perlakuan dan kontrol. Penelitian dilakukan di Kecamatan Arjasa Kabupaten Jember pada bulan Maret hingga Mei 2013. Hasil penelitian menunjukkan adanya peningkatan konsumsi bahan mentah sumber goitrogenik sebesar 25,98 gram. Namun, tidak terjadi peningkatan konsumsi sianida, justru menurun sebesar 9,09 miligram pada kelompok perlakuan. Hal ini terjadi karena pemilihan cara pengolahan yang tepat sesuai materi intervensi, yaitu beralih ke kulub dan rebus berkuah. Namun penurunan ini tidak signifikan (p = 0,56). Materi tentang GAKI dan cara mereduksi kadar sianida pada bahan pangan sumber goitrogenik sianida bisa dijadikan materi penyuluhan dalam program pencegahan GAKI di Kabupaten Jember. Jember still encounter the problem of nutrition iodine deficiency disorders (IDD), most of the district are included in the category of endemic areas. One reason is the cyanide goitrogenic factors that can interfere with the function of the thyroid hormone. Its presence in some commonly consumed vegetables society, causes the need for patterns of consumption and good processing in order to make it safe for consumption. Cyanide is a precursor thiocyanate which disrupt the formation of thyroid hormones through two pathways, active transport and interfere with the activity of thyroid peroxidase. This study aimed to change food pattern and way of processing goitrogenic cyanide food stuff through nutritional counseling and demonstration of food processing to reduce cyanide in goitrogenic food stuff. The research was a quasy-experimental study with pretest-posttest control design. The number of samples 196 housewives, consist of 98 people in the respective treatment groups and control. The study was conducted in the District Arjasa Jember between March and May 2013. The result showed presence of increased consumption of raw materials sources goitrogenic cyanide of 25.98 grams, was not followed by an increase in the consumption of cyanide, which has decreased by 9.09 miligram in the treatment group. This occurs because of the selection of appropriate food processing, switching to boil and blanching (kulub). However, this decrease was not significant (p = 0.56). The material on IDD and how to reduce levels of cyanide in the food source of cyanide can be used as material counseling in prevention programs IDD in Jember.
Co-Authors Abdul Salam M Sofro, Abdul Salam Abdul Salam Mudzakir Sofro, Abdul Salam Mudzakir Agus Siswanto Ahmad Hamim Sadewa Astuti, Herni Banudi, La Eny Catur, Eny Farida Wahyu Ningtyas Farida Wahyu Ningtyias, Farida Wahyu Imono A.D., Imono Jenita DT Donsu Juleka, Juleka M Noor Rochman Hadjam Madarina Julia Marsetyawan -, Marsetyawan Marsetyawan Marsetyawan Mufrod ., Mufrod Nyoman Kertia Poerwono Rahardjo Pramudji Hastuti, Pramudji Rahmat Hidayat Reppie, Maxie Roudy Retno Pangastuti Rochmach, Wasilah Siregar, Arief Kurniawan Suhaema Suhaema, Suhaema Susetyowati Wasilah Rochmah Yayi Suryo Prabandari