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All Journal JMM (Jurnal Masyarakat Mandiri) Biota Jurnal Ilmiah Medicamento PROFESSIONAL HEALTH JOURNAL KESANS : International Journal of Health and Science Abdinesia: Jurnal Pengabdian Kepada Masyarakat
Imam Syahputra Yamin
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Biota

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The Genetic Diversity of Macroalgae on The Ekas Coast of East Lombok Based on Chromatogram Data from PCR Ampification Baiq Repika Nurul Furqan; Imam Syahputra Yamin; Sri Sofiati Umami
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.417

Abstract

Macroalgae are multicellular photosynthetic organisms that live in water. Some species of macroalgae are very difficult to identify based on their morphology alone because they have great similarities. Therefore, identification of macroalgae diversity based on its genotype is very important to do in order to enrich the genetic information of macroalgae found on one of the beaches on the island of Lombok. This study aimed to analyze the genetic diversity of macroalgae from Pantai Ekas Beach, Jeowaru District, East Lombok, NTB using 18S rRNA gene markers. The research was carried out in 4 stages, namely sampling, morphological analysis of samples, isolation of total macroalgae DNA, isolation of genes by PCR amplification, agarose gel electrophoresis. Sampling was carried out at certain coordinates at east longitude and south latitude using the Ekas Beach quadratic transect method. The morphological analysis of the samples was guided by an identification key. Total DNA, macroalgae were isolated based on the spin column I DNA kit easy method. Genes were isolated by means of PCR amplification. Total DNA isolates and gene amplicons were confirmed by agarose gel electrophoresis. The results obtained were based on their morphology, all samples obtained were grouped into 9 macroalgae samples which could be divided into brown macroalgae (3 samples), green macroalgae (2 samples) and red macroalgae (4 samples). All samples have successfully isolated total DNA and its 18S rRNA gene which was confirmed by agarose gel electrophoresis chromatogram.
Hematological Test: Predicting Incidence Dengue Shock Syndrome Yamin, Imam Syahputra; Wahyono, Tri Yunis Miko
Biota Vol 14 No 2 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i2.387

Abstract

Dengue hemorrhagic fever (DHF) is an infectious disease caused by the Dengue virus. DHF cases in Mataram City from 2016 to 2020 tend to increase where most of the DHF patients are treated at the Mataram City Hospital. Death due to DHF infection mostly occurred in patients with Dengue Shock Syndrome (DSS) and mortality from DSS was reported to be 50 times higher than in DHF patients without DSS. Prompt diagnosis and appropriate treatment are important determinants for the prevention and control of dengue cases, so knowing the predictors of risk factors can prevent/reduce deaths from dengue virus. This study used a case-control design. Cases were DHF patients diagnosed with DSS by the treating doctor, while the controls were DHF patients diagnosed not DSS by the treating doctor. The research data was obtained from the results of laboratory tests stored in the medical records of patients treated at the Mataram City Hospital from January 2016 to December 2020. The design of the analysis was aimed at obtaining the odds ratio (OR) value followed by multivariate analysis to determine the risk factors that could detect DSS earlier. The variables that were statistically significant in the prediction of the final model were the increase in hematocrit value > baseline with OR= 17.1 (95% CI: 4.033-72.600), a decrease in platelet value < 100.000/µL with OR = 6 (95% CI). : 2,306-15,699), and decreased leukocyte value < baseline with OR= 5.1 (95% CI: 2,209-11,838). While the most dominant variable is the increase in hematocrit value > baseline with OR = 17.1 (95% CI: 4.033-72.600) and p value = 0.000.
Antibacterial Activity Test of Ethanol Extract of Red Macroalgae (Acanthopora Spicifera) Against Staphylococcus Aureus Bacteria Taibin, Ali; Yamin, Imam Syahputra; Nurul Furqan, Baiq Repika
Biota Vol 15 No 2 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i2.487

Abstract

Bacterial infection Staphylococcus aureus has become a significant global health problem, mainly due to increasing resistance to existing antibiotics. To overcome this problem, research is continuing to find new sources of natural antibacterial agents. Red macroalgae (Acanthopora sp.) has promising biological potential, including activity as an antimicrobial. This study aims to determine the effect and how much concentration of red macroalgae ethanol extract (Acanthopora sp.) which is close to the value of the inhibition zone of the positive control Amoxicillin in inhibiting bacterial growth S. aureus. This research is a laboratory experimental research. The stages of the research began with taking red macroalgae samples (Acanthopora sp.) collected from Pandanan beach, North Lombok Regency, West Nusa Tenggara by the method simple random sampling and identified morphologically, manufacture of simplicia, manufacture of red macroalgae ethanol extract (Acanthopora sp.) with 99.99% ethanol solvent (absolute p.a), phytochemical screening tests, and antibacterial activity tests using a Completely Randomized Design (CRD) were carried out by well diffusion method with concentration variations of 2%, 4%, 6%, 8% and 10%, Amoxicillin as positive control and aquadest as negative control and ethanol as solvent control. Antibacterial activity test results from the crude extract of red macroalgae (Acanthopora sp.) against bacteria S. aureus incubated during overnight (16-18 hours) in an incubator with a temperature of 37OC. The results showed that the phytochemical analysis of the ethanol extract of red macroalgae identified the presence of flavonoids and saponins which are known to have potential as antibacterial agents. The presence of these compounds supports the antibacterial activity of red macroalgae against S. aureus. Red macroalgae ethanol extract (Acanthopora sp.) has antibacterial activity against S. aureus. The antibacterial activity of the ethanol extract showed a concentration-response pattern, where the higher the concentration of the extract, the greater the inhibition zone formed. The concentration of 10% ethanol extract showed the largest inhibition zone with an average diameter of 23.5 mm with the sensitive category.
Bioactivity Diversity (Antibacterial and Antioxidant) of Macroalgae in the Ekas Beach Area, Lombok Nurul Furqan, Baiq Repika; Yamin, Imam Syahputra; Umami, Sri Sofiati
Biota Vol 17 No 1 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v17i1.489

Abstract

Macroalgae in the southern coastal area of Lombok has not been explored much in terms of bioactivity. The aim of this research is to explore the usefulness of 9 types of macroalgae on Ekas Lombok beach by searching for their bioactivity capabilities, whether each macroalgae has no bioactivity at all, has antibacterial and antioxidant activity, only has antibacterial activity but no antioxidant activity or vice versa and whether there are macroalgae that even have anticancer activity. The method used in this research consists of several steps: Macroalgae Sampling, Morphological Analysis of Macroalgae Samples, Isolation of active compounds from macroalgae and Cytotoxic Test on Breast Cancer Cells in Vitro which consists of 3 tests: Culture and sub culture of MCF-7 Breast Cancer Cells, Cell Viability and Cytotoxicity Test with MTT and DNA Isolation of MCF-7 Breast Cancer Cells. The research results show that the macroalgae with the best antibacterial activity is the red macroalgae Acanthophora spicifera and the macroalgae with the best antioxidant activity is the green macroalgae Ulva reticulata.
Co-Authors Antari, Gladeva Yugi Baiq Desy Ratnasari Baiq Desy Ratnasari Baiq Repika Nurul Furqan Diah Miftahul Aini Effendi, Julham Febrina Sulistiawati Manuaba, I.B.G. Fajar May Hardianti Muhammad Syamsussabri Nanda Berliana Tania Fidzikri Novia Arista Sanjaya, I.N Hariyasa Sri Sofiati Umami Sri Sofiati Umami, Sri Sofiati Taibin, Ali Tri Yunis Miko Wahyono Zulhakim, Zulhakim