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All Journal Jurnal Kesehatan Prima Jurnal Analis Medika Biosains (JAMBS) The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Journal of Indonesia Laboratory Students (JILTS)

Maruni Wiwin Diarti

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Author-ID : 2390830

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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SENYAWA PENGKUPLING α-NAFTHILAMIN UNTUK VALIDASI METODE SPEKTROFOTOMETRI PENENTUAN NITRIT (〖〖NO〗_2〗^-) DI DALAM AIR Maruni Wiwin Diarti; I Gusti Ayu Nyoman Danuyanti; I Gede Billy Sumantri
Jurnal Kesehatan Prima Vol 9, No 1 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Validation of analytical methods need to be conducted to determine the feasibility of the method when the method is newly developed or modified. Determination of nitrite in water-based Cupling diazotized reaction has been developed in various fields. Many modifications have been carried out primarily in the selection of the source of the diazonium salt compound or compounds that can cupling. The this study was to determine the validity of the determination of nitrite in water by spectrophotometric method cupling compound α-nafthilamin. This study is a pre-experiments with experimental units and large units based guidelines experimental validation of analytical methods. The results show the value of 0.995 linearity, limit of detection (LOD) 0.039 ppm, 0.882 sensitivity, precision 1.38%, and 100.37% accuracy rate. The conclusion of this study is the method of spectrophotometric determination of nitrite in water using compound α-nafthilamin cupling valid for determining the concentration of nitrite in the water with a minimum detection limit of 0.039 ppm.

SENSITIVITAS MEDIA OGAWA DAN MEDIA LOWENSTEIN JENSEN TERHADAP HASIL PERTUMBUHAN KUMAN MYCOBACTERIUM TUBERCULOSIS Pancawati Ariami; Maruni Wiwin Diarti; Yunan Jiwintarum
Jurnal Kesehatan Prima Vol 8, No 2 (2014): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Referring to the national TB control program, the diagnosis made by microscopic examination of sputum by direct and definitive diagnosis by culture examination. Direct sputum examination is the gold standard method of microscopic examination of a recognized WHO TB and confirmation done by the method of culture. Research "Sensitivity Test Using Ogawa Medium and Lowenstein Jensen Medium Againts Micobacterium tuberculosis Bacteria Growth Results ", performed to identify and compare the results of Ogawa medium and culture on Lowenstein-Jensen medium and analyze the sensitivity between the two medium. A descriptive observational study was conducted. Study sample is a sample saturated with a consecutive sampling. Sample size retrieval technique based on sample collection time, which is June 8 through July 18, 2011, a total of 53 samples. Research is to test the sensitivity variables as independent variables and the growth of Mycobacterium tuberculosis bacteria on the Ogawa medium and LJ medium as a bound variable. The results of the study, of 53 samples, 21 (39.6%) samples were negative, so that 32 (60.4%) samples tested positive for M tuberculosis and the total growth of 36 samples (67.9%). Lowenstein Jensen (LJ) medium is more sensitive than the Ogawa medium, both in terms of speed of growth and in the number of colonies that grow. For the determination of Mycobacterium species needs to be done other tests to confirm. Culture results can also be used for comparison using the PCR method, OAT resistance by culture methods.

THE ALTERNATIVE DILUTE SOLUTION OF NaCl 0.9% AT THE GIEMSA STAINING ON THE INVESTIGATION THE MORPHOLOGY OF SPERMATOZOA Maruni Wiwin Diarti; Erlin Yustin Tatontos; Aden Turmuji
Jurnal Kesehatan Prima Vol 10, No 2 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract : The quality of staining, the morphology of spermatozoon applays The Giemsa dye, is strongly influenced by the type of Giemsa thinner. The terms of diluent that can be used is having a buffer property. The solution of NaCl 0.9 %, besides having buffer properties also has a cheaper price. The research is a descriptive study that aims to determine NaCl 0.9% as the alternative of diluting solution at Giemsa staining on morphological examination of spermatozoon. This study was conducted to compare the quality of spermatozoon morphology staining by using Giemsa which was diluted with 0.9% NaCl solution and the solution of Phosphate Buffer. The result of the study indicated The Giemsa staining with NaCl 0.9% solution, is obtained the outstanding spermatozoon morphology staining absorption was 71.625% and the poor spermatozoon morphology staining absorption was 28.375%. The results of study which was applied the Phosphate Buffer solution, was obtained the outstanding spermatozoon morphology staining absorption was 81.563% and the poor spermatozoon morphology staining absorption was 18.438%. The NaCL 0.9% can be used as an alternative dilute solution at Giemsa staining on morphological examination of spermatozoon.

The Result Discrepancies between Histological and PCR Method for Detecting Helicobacter pylori in Patients with Dyspepsia due to Inappropriate Preparation before Endoscopy Maruni Wiwin Diarti; Haris Widita; Soewignjo Soemohardjo; Weny Astuti; Troef Sumarno; Yunan Jiwintarum; Zainul Mutaqin; Retno Handayani
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 10, ISSUE 2, August 2009
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/102200946-50

Background: False negative result of Helicobacter pylori (H. pylori) detection in gastric tissue can be due to inappropriate preparation before endoscopy. The objectives of this study is to compare the result of H. pylori detection in gastric biopsy by histological method and ure C polymerase chain reaction (PCR) in patients with dyspepsia who underwent upper gastrointestinal (GI) endoscopy without preparations other than six hours fasting before endoscopy. Method: We obtained 156 paraffin blocks of gastric endoscopic biopsy samples, taken from antrum and corpus of patients with dyspepsia who underwent endoscopic examination at the Endoscopy Unit of Biomedika hospital, Mataram. All biopsy samples were stained with Hematoxylin and Eosin for tissue diagnosis and Giemsa stain for detecting H. pylori Ure C PCR were done on all blocks. Cag PCR were performed on all Ure C PCR positive samples. Results: Of 156 paraffin blocks, only 17 blocks (10.9%) were positive for H. pylori by histological examination. All of the 17 samples showed positive results on PCR method. Of 156 paraffin blocks, positive results were found in 73 patients (45.9%) by ure C PCR method. The PCR method has increased the positivity rates of H. pylori more than four times compared to histological method. This study showed that the rate of cag a was 63.0%. Conclusion: Ure C PCR is superior to histological examination in patients who did not stop consuming acid supressor drug and antibiotic two weeks prior to endoscopy. This phenomenon can be explained by the change of spiral form into coccoid form of H. pylori, which is hardly detected using Giemsa stain. Keywords: Helicobacter pylori, histology, ureC, Cag a, PCR

Hubungan antara Lama Terinfeksi Virus Penderita Hepatitis B dengan Indeks Eritrosit (MCV,MCH,MCHC) Septiani, Erna; Maruni Wiwin Diarti; Erlin Yustin Tatontos; Fihiruddin
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 2 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i2.166

Background: Hepatitis B is an infectious disease that attacks the liver caused by the Hepatitis B virus. Hepatitis B virus infection is very infectious and is the main cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Hepatitis B can cause liver cirrhosis, where there is a change in the structure of the liver parenchyma resulting in a decrease in erythrocyte index levels. Objective: To determine the relationship between the duration of viral infection in hepatitis sufferers and the erythrocyte index (MCV, MCH, MCHC) Method: This research is an analytical observational study with a cross sectional approach and accidental sampling technique Results: Based on the results of the Kruskall Wallis test, the relationship between the duration of infection with the hepatitis B virus and the erythrocyte index (MCV, MCH, MCHV) resulted in MCV 75.50, MCH 17.97, MCHC 43.03 with a p value = 0.000 < 0.05. so it can be concluded that there is a relationship between the duration of infection with the hepatitis B virus and the erythrocyte index (MCV, MCH, MCHV) Conclusion: From the results of the analysis carried out, there is a relationship between the duration of infection with the hepatitis B virus and the erythrocyte index (MCV, MCH, MCHV)

Co-Authors Aden Turmuji Anas Fadli Wijaya Anggun Sari Mianti Awan Dramawan Edy Kurniawan Erlin Yustin Tatontos, Erlin Yustin Fahrudin Ahad Fihiruddin Haerul Anam Haris Widita Hesti Suliastiningsih I Gede Billy Sumantri I Gusti Ayu Nyoman Danuyanti Ida Ayu Reka Iswari Pauzi Nurhidayati Nurhidayati Pancawati Ariami Retno Handayani Rohmi Rohmi Rohmi Rohmi Santy Pristianingrum Septiani, Erna Siti Rif’ah Sabariah Soewignjo Soemohardjo Troef Sumarno Urip Urip Weny Astuti Yunan Jiwintarum Yuri Syaffitri Kinanti Achmad Zaenal Fikri Zainul Mutaqin

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