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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia Biotropika Journal of Biomedicine and Translational Research Bioma : Jurnal Biologi Indonesia Global Health Management Journal Acta Biochimica Indonesiana Majalah Kedokteran Andalas Medika Kartika : Jurnal Kedokteran dan Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Tunas Medika Jurnal Kedokteran & Kesehatan JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia
Beti Ernawati Dewi
Departemen Mikrobiologi Fakultas Kedokteran Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : Global Health Management Journal

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The Effectiveness of Cassava Leaf (Manihot esculenta C.) Flavonoid Quercetin as Dengue Virus-1 Antivirus in Vitro Rayasari, Husnaya; Apriyanto, Dadan Ramadhan; Brajawikalpa, Rama Samara; Dewi, Beti Ernawati
GHMJ (Global Health Management Journal) Vol. 7 No. 3s (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-741086

Abstract

Background:  Dengue fever is a mosquito-borne disease that can cause severe symptoms and death, placing high burden on healthcare systems in tropical regions. Currently, there are no antiviral treatments. It has been proven that synthetic quercetin able to improve body performance and reduce the risk of infection as well as inhibit DENV replication. Cassava leaves (Manihot esculenta C.) contain anthocyanins (flavonoids) such as quercetin and it shows potential as antiviral agent as the synthetic as well.    Aims: To describe the levels of self-compassion and procrastination among medical students and to examine how these factors relate to academic performance. Methods: This experimental study used post-test only with control group design consisted of 13 treatment groups and 1 control group. Treatment group were divided into 6 different concentrations for inhibition test (50 µg/mL, 25 µg/mL, 12.5 µg/mL, 6.25 µg/mL, 3.125 µg/mL, 1.5 µg/mL) and 7 different concentrations  for cytotoxicity test (80 µg/mL, 40 µg/mL, 20 µg/mL, 10 µg/mL, 5 µg/mL, 2.5 µg/mL, 1.25 µg/mL). The control group was a negative control treated with 0.2% DMSO. Inhibition was assessed by Focus Forming Unit Assay and cytotoxic was assessed by number of Microtiter Tetrazolium Assay (MTT Assay). The statistical analysis used in this study were the Shapiro-Wilk test for normality test, Kruskal-Wallis test for hypothesis test, and post-hoc test to determine whether the groups studied in this research had significant differences from each other. Results: The CC50 and IC50 values of quercetin of cassava leaf (Manihot esculenta C.) were found to be 3.44 μg/mL and 0.25 μg/mL, respectively, with an SI value of 123. Based on these results, it can be concluded that the flavonoid quercetin of cassava leaf (Manihot esculenta C.) exhibits selective properties in inhibiting the replication of DENV-1. Statistical analysis showed non-normal distribution (P<0.05), hypothesis test was accepted (P<0.05), and no significant differences between concentrations in the post-hoc test. Conclusion: Quercetin of cassava leaf (Manihot esculenta C.) is effective as an antiviral agent against dengue serotype 1 strain New Guinea C in vitro. Received: 25 September 2024  |  Reviewed: 15 October 2024  |  Revised: 30 November 2024  |  Accepted: 15 December 2024.   Dimension Badges:
Inhibition Test of Cassava Leaves (Manihot Esculenta Crantz) Flavonoid Nicotiflorin on Replication of Dengue Virus Serotype 1 in Vitro Yulianti, Selsa; Apriyanto, Dadan Ramadhan; Brajawikalpa, Rama Samara; Dewi, Beti Ernawati
GHMJ (Global Health Management Journal) Vol. 8 No. 2 (2025)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-821192

Abstract

Background:  Dengue fever is a disease caused by the bite of Aedes aegypti mosquitoes infected with the dengue virus. Dengue virus infection can lead to hemorrhagic fever and even death. Currently, treatment for dengue infection is supportive, as there are no commercially available antiviral drugs. Nicotiflorin, a compound found in cassava leaves (Manihot esculenta Crantz), has shown potential as an antiviral agent against dengue. Aims: This study aimed to determine the inhibitory effect of the flavonoid nicotiflorin, derived from cassava (Manihot esculenta Crantz) leaves, as an antiviral agent against dengue virus serotype 1 (DENV-1) in vitro. Methods: A post-test-only control group design was utilized in this experimental research, comprising one control group alongside thirteen treatment groups. For the cytotoxicity assessment, the treatment groups were exposed to seven varying concentrations (1.25 µg/mL, 2.5 µg/mL, 5 µg/mL, 10 µg/mL, 20 µg/mL, 40 µg/mL, and 80 µg/mL), while six different concentration levels (1.5 µg/mL, 3.125 µg/mL, 6.25 µg/mL, 12.5 µg/mL, 25 µg/mL, and 50 µg/mL) were employed in the inhibition assay. The control group received 0.2% DMSO as a negative control. Antiviral inhibition was assessed using the Focus Forming Unit (FFU) Assay, while cytotoxicity was evaluated using the Microtiter Tetrazolium Assay (MTT Assay). Statistical analyses included the Shapiro-Wilk test for normality, the Kruskal-Wallis test for hypothesis testing, and post-hoc analysis to assess significant differences among treatment groups. Results: The CC₅₀ and IC₅₀ values of nicotiflorin from cassava leaves were determined to be 19.24 μg/mL and 0.9550 μg/mL, respectively, yielding a Selectivity Index (SI) of 20.14. These findings indicate that the flavonoid nicotiflorin from cassava leaves exhibits selective antiviral activity against DENV-1 replication. Statistical analysis revealed a non-normal data distribution (P < 0.05), a significant difference among groups based on the Kruskal-Wallis test (P < 0.05), and no statistically significant differences among specific concentrations in the post-hoc test. Conclusion: Nicotiflorin from cassava leaf (Manihot esculenta Crantz) has inhibitory activity on the replication of dengue virus serotype 1 strain in vitro.
Co-Authors . Andriansjah Agus Syahrurachman Agustin Iskandar Aldo Ferly Andi Yasmon Apriyanto, Dadan Ramadhan Aryati Aryati Budiman Bela Chie Aoki-Utsubo Cita Christine Mayorita DEKA LARASATI Desti, Hidayati Dewi Wulandari Dewi Wulandari Dewijanti, Indah Dwiatmi Eduardus Gilang Putra Eleanor Louana Urfa Elisabeth D. Harahap EVA DAMAYANTI Fera Ibrahim Fithriyah Fitriyah Sjahta Hak Hotta Hardi Darmawan Heni Pujiastuti Herdiman T. Pohan Kartika, Aisya Alma Asmiranti Leonard Nainggolan Lia Meilawati Linlin Haeni Marissa Angelina Mirawati Sudiro Nadia Tita Indriasti Nikki Aldi Massardi Nugraheni, Enny Oktania Sandra Puspita OKTIVIA CHANDRA MUSTIKA Pramita G. Dwipoerwantoro Putra, Eduardus Gilang Rahajuningsih Dharma Rama Samara Brajawikalpa Rayasari, Husnaya Rianto Setiabudy Rizka Andhitia Mentari Putri Safrullah, Muhamad Iqbal Santi, Mei Ria Settrin Chenderawasi SJATHA, FITHRIYAH Soemanadi Soemanadi Sri Hartati Sri Hartati Suhendro Suhendro Syamsiar, Syamsiar T. Mirawati Sudiro TJAHJANI MIRAWATI SUDIRO Tjahjani Mirawati Sudiro Tjahjani Mirawati Sudiro VIVI SETIAWATY Yulianti, Selsa YULIANTY MUHAYAR Zanjabila, Sabighoh