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All Journal Jurnal Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Jurnal Agro Veteriner
Eka Pramyrtha Hestianah
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Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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Bakteri Legionella pneumophila Terdeteksi pada Air Kolam Renang di Kota Surabaya dengan Nested Polymerase Chain Reaction (LEGIONELLA PNEUMOPHILA BACTERIADETECTED IN SWIMMING POOL WATER OF SURABAYA BY USING NESTED POLYMERASE CHAIN REACTION) Eduardus Bimo Aksono; Ana Adelina Farahdiba; Eka Pramyrtha Hestianah
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.466 KB) | DOI: 10.19087/jveteriner.2017.18.2.221

Abstract

Legionella pneumophila is a Gram-negative bacillus that causes nosocomial and community-acquired pneumonia. The aim of this research was to detect the presence of bacteria of L. pneumophila species in the swimming pools water of Surabaya city by using nested Polymerase Chain Reaction (PCR) assay of a specific gene for L. pneumophila (mip gene). This study used purposive sampling method. A total of 10 water samples were collected from five swimming pools consisting of 200 mL water for each swimming pool. The results showed that of 10 samples tested by nested PCR, one sample was positive for L. pneumophila, and nine samples were negative. L. pneumophila were found in pool water samples with a higher temperature (>30ºC).Serogrouping analysis of positive sample that L. pneumophila bacteria detected in the water sample of swimming pool in Surabaya was L. pneumophila serogroup 9 (98%) and serogroup 10 (98%). L. pneumophila detection of bacteria is expected to raise the awareness of physician and microbiologists about the transmission of L. pneumophila and will also be useful for controlling the agents. ABSTRAK Legionella pneumophila adalah bakteri Gram-negatif berbentuk batang yang dapat menyebabkan penyakit nosokomial dan pneumonia. Tujuan penelitian ini adalah untuk mendeteksi keberadaan bakteri L. pneumophila pada air kolam renang di Kota Surabaya dengan menggunakan nested Polymerase Chain Reaction (PCR) berbasis gen spesifik L. pneumophila (mip gene). Penelitian ini menggunakan metode purposive sampling. Sebanyak sepuluh sampel diambil dari lima kolam renang. Sampel diambil sebanyak 200 mL dari air kolam renang di setiap lokasi. Hasil dari 10 sampel yang diuji menggunakan nested PCR, satu sampel menunjukkan hasil positif untuk L.pneumophila, dan sembilan sampel menunjukkan hasil negatif. Bakteri L. pneumophila ditemukan pada sampel air kolam dengan suhu yang lebih tinggi (>30ºC). Satu sampel positip tersebut ketika dilanjutkan terhadap analisis serogrup terlihat bahwa bakteri L. pneumophila yang terdeteksi pada air kolam renang di Kota Surabaya termasuk L. Pneumophila serogrup 9 (98%) dan serogrup 10 (98%). Terdeteksinya bakteri L. pneumophila ini diharapkan dapat meningkatkan kesadaran dokter dan ahli mikrobiologi tentang penyebaran L. pneumophila dan juga bermanfaat untuk mengontrol agen Legionellosis.
The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

Abstract

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.
Histological Features of the Duodenum of Mice (Mus musculus) Treated with Liquid Probiotic Isolates of Bacillus coagulans, Bacillus subtilis, and Saccharomyces cerevisiae Devi Sri Rohmani; Mohammad Anam Al Arif; Sri Agus Sudjarwo; Mohammad Sukmanadi; Eka Pramyrtha Hestianah; Widya Paramita Lokapirnasari
Jurnal Agro Veteriner Vol. 7 No. 2 (2024): Journal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i2.57620

Abstract

This study aims to determine the histological description of the height and width of the duodenal villi of mice (Mus musculus) treated with liquid probiotic isolates of Bacillus coagulans, Bacillus subtilis, and Saccharomyces cerevisiae. Histological examination of the duodenum of mice was carried out by making histological preparations from the height and width of the duodenal villi which were observed under a microscope with 100x magnification. Statistical analysis in this study used One-Way ANOVA and Duncan's test. The results showed that the control group and the treatment group had significantly different height and width of the duodenal villi (p<0.05). The conclusion of this study is that the administration of isolates Bacillus coagulans, Bacillus subtilis, and Saccharomyces cerevisiae can increase the height and width of the duodenal villi of mice and the P2 group with a dose of 0.27 mL/kg BW has the greatest average value for the height and width of the duodenal villi with an average height of 360.80 μm and an average width of 152.30 μm.
Spermatozoa motility and viability of turkey (Meleagris gallopavo) semen in egg yolk nanoparticles-phosphate buffered saline extender at 3-5°C storage Nabila Ayu Saraswati; Herry Agoes Hermadi; Tri Wahyu Suprayogi; Tatik Hernawati; Eka Pramyrtha Hestianah
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.129-138

Abstract

This study aimed to evaluate the spermatozoa motility and viability of turkey semen extended with egg yolk nanoparticles-PBS extender at various concentrations, stored at 3-5°C. Semen was collected from two turkeys and divided into four treatment groups. Group T0 consisted of fresh turkey semen extended with 20% (v/v) fresh egg yolk in phosphate-buffered saline (PBS). Groups T1, T2, and T3 consisted of fresh turkey semen extended with 5, 10, and 20% (v/v) egg yolk nanoparticles in PBS, respectively. Extended semen was stored in a refrigerator (3-5°C), and progressive spermatozoa motility and viability were evaluated every two hours until spermatozoa motility decreased to 40%. The results showed that turkey semen in T3 extender containing 20% ​​egg yolk nanoparticles in PBS was able to maintain spermatozoa motility for up to eight hours, better than T0 and T2 extenders which supported spermatozoa motility for six hours. In contrast, T1 extenders only support spermatozoa motility for four hours. T3 extenders were also able to maintain spermatozoa viability for up to eight hours, while T0, T1 and T2 extenders could only support viability for 6 hours. The extender with 20% egg yolk nanoparticles in PBS was significantly better (p <0.05) in maintaining spermatozoa quality compared to those with 5 and 10% egg yolk nanoparticles, as well as the extender with 20% fresh egg yolk. In conclusion, 20% egg yolk nanoparticles in PBS semen extender was effective in maintaining the motility and viability of turkey (Meleagris gallopavo) spermatozoa stored at 3-5°C for up to eight hours.
Toxoplasma Oocyst Contamination in Water Sources in Goat-Sheep Farming In Sidoarjo District Mufa, Ramy Inas Mahirah; Eka Pramyrtha Hestianah; Lucia Tri Suwanti; Hani Plumeriastuti; Widya Paramita Lokapirnasari; Tony Hartono; Rina Vitriasari; Retno Yuli; Galaxy Guardian; Heni Puspitasari; Mufasirin
Journal of Parasite Science Vol. 9 No. 1 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i1.67669

Abstract

Toxoplasmosis is one of the zoonotic diseases caused by the protozoa Toxoplasma gondii. The disease infects warm-blooded animals including humans. The purpose of this study was to determine the contamination of Toxoplasma oocysts in water sources in goat-sheep farms in Sidoarjo Regency. A total of 63 water samples were used for the study. Water samples were taken from 5 sub-districts that have many goat-sheep farms, each sub-district has 2 villages, with each village having 6-7 samples. The examination used the floating method and was examined under a microscope with a magnification of 400x. The results of the examination found 21 samples of Toxoplasma gondii oocysts or 33.33% (21/63). The morphology of T.gondii oocysts is round, ovoid or ellipsoid with sizes varying between 8.02 - 12.72 micrometers. Preventive measures are needed by treating water before giving it to livestock.
The effect of addition of melon (Cucumis melo L.) flesh juice into Lactated Ringer's-egg yolk extender on spermatozoa plasma membrane integrity and spermatozoa morphological abnormalities of semen of native rooster Pitaloka, Dinda Galuh; Mas'ud Hariadi; Suherni Susilowati; Budi Utomo; Tatik Hernawati; Eka Pramyrtha Hestianah; Samuel Inioluwa Akeju
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.49-58

Abstract

This study aims to determine the effect of adding melon (Cucumis melo L.) juice to Lactated Ringer's-egg yolk extender on the intact plasma membrane (IPM) and morphological abnormalities of native rooster spermatozoa. Five ejaculate each from two native roosters (Gallus gallus domesticus) were divided into four equal volumes. Ejaculate was extended 1:10 (v/v) in Lactated Ringer's-egg yolk without or with the addition of of 2, 4, and 8% (v/v) melon flesh juice, respectively for groups T0, T1, T2, and T3. Extended semen was stored in a refrigerator (5°C) and evaluated every two hours. The results showed that IPM of all groups decreased (p <0.05) consistently, lasting more than 40% for six hours in the T0 and T1 groups and four hours in the T2 and T3 groups. The percentage of spermatozoa morphological abnormalities in all groups stored for six hours was higher (p <0.05) than before storage. However, it was not significantly different (p >0.05) from that which had been stored for two and four hours. It was concluded that native rooster semen extended in Lactated Ringer's-egg yolk without (group T0) or with 2% melon flesh juice (group T1) and stored at 5°C retained spermatozoa plasma membrane integrity of more than 40% and morphological abnormalities of less than 20% for six hours. While the addition of 4 % (T2) and 8% (T3) melon flesh juice maintained the percentage of IPM and spermatozoa morphological abnormalities for only four hours.
Tomato (Lycopersicon esculentum Mill.) juice restored the number of Leydig cells, and the diameter of the seminiferous tubules of mice (Mus musculus) exposed to lead acetate Revina Ayu Septiani; Iwan Sahrial Hamid; Emy Koestanti Sabdoningrum; Anwar Ma'ruf; Eka Pramyrtha Hestianah; Maslichah Mafruchati
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.123-129

Abstract

Lead is a harmful pollutant from engine exhaust that causes free radicals and has detrimental effects on the testicular tissue. This study aimed to determine the effects of tomato juice on the number of Leydig cells and the diameter of the seminiferous tubules of mice exposed to lead acetate. Twenty-five male mice were divided into five groups. Mice in the control (C-) group were given placebos. Meanwhile, mice in C+, T1, T2, and T3 groups were exposed to lead acetate at a dose of 100 mg/kg BW/day for 14 days and given tomato juice respectively at 0, 0.16, 0.32, and 0.64 mL/day from day 8 to day 35. On day 36, all mice were sacrificed, and the testes were collected for histological preparation. The result showed that lead exposure in the C+ group decreased (p <0.05) in the number of Leydig cells and the diameter of the seminiferous tubules compared to the mice in group C-. Administration of tomato juice in groups T1, T2, and T3 increased (p <0.05) the number of Leydig cells and the diameter of seminiferous tubule compared to the mice in the C+ and C- groups. However, tomato juice administration to the T3 group decreased the number of Leydig cells and the diameter of the seminiferous tubules (p <0.05) compared to the T2 group. In conclusion, an effective dose of 0.32 mL/day of tomato juice restored Leydig cell number and seminiferous tubules diameter in mice exposed to lead acetate.
Effect of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic cells and Leydig cells of gentamicin-induced rats Ngizzah, Nailul; Wurlina Wurlina; Poedji Hastutiek; Iwan Sahrial Hamid; Eka Pramyrtha Hestianah; Lita Rakhma Yustinasari
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.99-106

Abstract

This study aimed to determine the effect of the administration of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic and Leydig cells of gentamicin-induced rats (Rattus norvegicus). This study used 25 white male rats divided randomly into five groups. The rats were injected with Gentamicin 5 mg/kg bw/day subcutaneously for 14 days, except for the negative control group (NCG). During the next 14 days, the rats were given oral moringa leaf extract of 200, 316, and 500 mg/kg bw/day respectively for E200, E316, and E500 groups. The data were analyzed with one-way ANOVA followed by Duncan's test. The results showed that the number of spermatogonia, spermatocytes, and spermatids in NCG was higher (p <0.05) than in the other groups. The number of spermatogonia, spermatocytes, and spermatids in the E316 group was higher (p <0.05) than in PCG. The number of spermatogonia in the E500 group was higher (p <0.05) than in the E316 group, but the number of spermatocytes and spermatids in the E316 group was similar (p >0.05) to the E500 group. The number of PCG Leydig cells was the smallest (p <0.05) compared to the other groups. The administration of Moringa leaves extract in the E200 and E316 groups increased (p <0.05) the number of Leydig cells compared to PCG. The number of Leydig PCG cells in the E500 group was not significantly different (p >0.05) from that of the NCG. It could be concluded that administration of Moringa oleifera leaves extract restored the number of spermatogenic and Leydig cells of gentamicin-induced rats.
Co-Authors Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Ana Adelina Farahdiba Annisa Nur Fitria Rahma Anwar Ma'ruf BUDI UTOMO Devi Sri Rohmani Emy Koestanti Sabdoningrum Galaxy Guardian Hani Plumeriastuti Heni Puspitasari Herry Agoes Hermadi Iwan Sahrial Hamid Kadek Rachmawati Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Mas'ud Hariadi Mohammad Sukmanadi Mufa, Ramy Inas Mahirah Mufasirin Nabila Ayu Saraswati Ngizzah, Nailul Nove Hidajati Pitaloka, Dinda Galuh Poedji Hastutiek Retno Yuli Revina Ayu Septiani Rimayanti Rimayanti Rina Vitriasari Samuel Inioluwa Akeju Sherina Lashita Candrakirana Sri Agus Sudjarwo Suherni Susilowati Tatik Hernawati Tony Hartono Tri Wahyu Suprayogi Widya Paramita Lokapirnasari Wurlina, W Yustinasari, Lita Rakhma