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All Journal Jurnal Veteriner JOURNAL OF COASTAL DEVELOPMENT BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Aquaculture Journal Journal of Aquaculture and Fish Health PENA AKUATIKA : JURNAL ILMIAH PERIKANAN DAN KELAUTAN
slamet budi prayitno
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Social Sciences Veterinary

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Journal : JOURNAL OF COASTAL DEVELOPMENT

 1 
THE PERFORMANCE OF BI-FILTER ON THE PRODUCTION AND DISEASE INCIDENCE OF PRAWN (PENAEUS MONODON FAB.) Slamet Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 4, No 1 (2000): Volume 4, Number 1, 2000
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

It has been long known that the most potent factor that influences prawn production was unfavorable environment due to industrial, agriculture and habitat wastes as well as improper application of good husbandry management. This research was designed to study the effect of biological filter on the growth of tiger prawn, water qualilv, and disease incidence. 7`wo sets of experiments, namely field stddy and laboratory experiment were conducted . Field survey was carrted out to collect field data on actual husbandry practices, production, water and soil quality. Laboratory experiment was aimed at evaluating the efhctiveness of biologicalfilter and disease incidence. Juvenile prawn e (j 5 gr.) at a density of 15 animals/M2; 20 animals/M2, and 25 animals/M2 were used as experimental animals. The observed parameters were field productivity, growth, survival rate, water quolitv, bacterial counts, soil quality and disease incidence. Experiments were carried in 3 replicates. Field survey showed that prawn production decreased steephr at the third production cycle as low as 627.65 kg/Ha compared to the first cycle (1893. 28 kg/lla}. Most prawn were weak, and very vulnerable to diseases. Bacterial count was more than I0Jcfii/ml. Water quality rapidly declined as prawn grew especially during the second and third cycles. Laboratory experiment with biologicalfilter in contrast demonstrated better water quality and healthier prawn,. although water quality declined slightly along with both the age and density. The growth rate was better than during field trials; among three treatments there were signdicant dyferences; the survival rate was best at density of I 5 animals/M2 but, the best estimated production was reached by density of 20 animals/M2 producing 2645 kg/Ha/cycle without any problems for around 4 cycles. Disease incidence and parasites infestation was undetected in the tank during the experiment.
THE EFFECT OF DIFFERENT C:N AND C:P RATIO OF MEDIA ON THE CONTENT OF POLYHYDROXYBUTYRATE IN BIOFLOC INOCULATED WITH BACTERIUM Bacillus cereus Supono .; Johannes Hutabarat; Slamet Budi Prayitno; YS Darmanto
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 2 (2013): VOLUME 16, NUMBER 2, YEAR 2013
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Biofloc technology  has  added values in aquaculture management, both in water quality  management and feeding  management.  As an optional feed, biofloc is capable to enhance growth due to high protein content.  Bacteria, main component biofloc, can produce polyhydroxybutyrate (PHB) as reserve of energy and growth accelerator for fish. The aim of the research were to study the effect of the different C:N and C:P ratio of media on the content of polyhydroxybutyrate in biofloc and to determine optimum media to produce high polyhydroxybutyrate content in biofloc. The experiment was arranged in factorial  with completely randomized design  in  three replications.  Treatments were C:N ratio of 15, 20, 25 and C:P ratio of 75, 100, and 125. The result showed that C:N ratio and C:P ratio of media and their interaction  affect the content of polyhydroxybutyrate in biofloc.  C:N ratio of 20 and C:P ratio of 125 resulted in most polyhydroxybutyrate (29.25±7.376 mg g-1 biofloc dry weight). Ratio of  C:N  of media gave linier and quadratic responses and C:P ratio of media gave linier one. Optimum polyhydroxybutyrate production was obtained at C:N ratio of 20.9 and C:P ratio of  125 resulting in 29.66 mg  g-1 biofloc dry weight (2,97%)
APPLICATION OF REPETITIVE SEQUENCE-BASED PCR ON THE RICHNESS OF VIBRIO ON THE TIGER SHRIMP (Penaeus monodon Fab.) S arjito; Nestin E.W. Ningrum; Ocky Karna Radjasa; Slamet Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

A molecular-based study was conducted to estimate the richness of the Vibrio on  tiger shrimp (Penaeus monodon Fab.) from brackish water pond of Mororejo, Kendal. Tiger shrimps were collected from the extensive brackish water pond and a total of 22 isolates were obtained from hepatopancreas and telson of tiger shrimp. Based on the repetitive sequence-based polymerase chain reaction (rep-PCR), it was found that two groups of Vibrio. To investige the efectiveness of rep-PCR in estimating the richness of Vibrio on tiger shrimps, three isolates  (JTW 01, JTW 03 and JTW 06) were chosen for further investigation. On the basis of sequence analysis, the result showed  that the JTW 01, JTW 03 and JTW 06 were closely related to Vibrio sp. Absa7 clone 423.1, Vibrio splendidus and Vibrio splendidus, respectively.  The result proved that two assosiated of Vibrio on tiger shrimp were Vibrio sp. Absa7 clone 423.1 and Vibrio splendidus. Therefore  the present study highlights the effectiveness of rep-PCR in rapid grouping and estimating the richness of Vibrio on  tiger shrimp.
THE EFFECTIVENESS OF Acanthus Ilicifolius IN PROTECTING TIGER PRAWN (Penaeus monodon F.) FROM Vibrio harveyi INFECTION Gina Saptiani; Slamet Budi Prayitno; Sutrisno Anggoro
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 2 (2012): Volume 15, Number 2, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Sea holly (Achantus ilicifolius) is a mangrove plant that often used by coastal society as a traditional medicine.  It is potential to be developed as the sources of pharmaceutical products. This study aims at assessing the effectiveness of sea holly leaves as antibacterial agent and as an agent to enhance the durability of shrimp against Vibrio harveyi.  First, dried sea holly leaves were extracted with methanol, after that, it was fractionated with silica gel column method using solvent n-hexane, ethyl acetate, and n-butanol.  The treatments given to tiger prawn were crude extract, the fraction of ethyl acetate, and n-butanol, and it was given by immersion.   Next, the challenge test was conducted toward Vibrio harveyi.  As the result, the extract and the leaves of A. ilicifolius possess activities of inhibiting the growth of V. harveyi in vivo, reducing the prevalence of attacks and improving survival of prawn. In general, based on clinical symptoms and pathological anatomy, n-butanol fraction of the A.  ilicifolius leaves possess the best protection, along with ethyl acetate fraction and the crude.
DETERMINATION OF MERCURY’S BIOACCUMULATION FACTOR IN MILK FISH (Chanos chanos) OF SEMARANG MUNICIPALITY FISHPONDS USING NEUTRON ACTIVATION ANALYSIS Slamet Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 3, No 3 (2000): Volume 3 Number 3 Year 2000
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Abstract

Determination of bioaccumulation factor of mercury (200Hg) in chanos chanos of Semarang municipality fishponds has been carried out by applying the neutron activation analysis (NAA) and gamma spectrometry analysis. The gamma spectrometry technique was employed to analyze the gamma radiation exposure from activated samples. The heavy metal 200 Hg can be identified in water and milk fish (Chanos chanos), along with natural radionuclides 40K in water. The concentration of Mercury in the water at the 3 villages was 0.950 10-6 ppm, 284 10-6, and 350 10-6 ppm respectively. Whilst mercury concentration in the milk fish was 186 10-3, 068 10-3, and 098 10-3 ppm for Mangunharjo, Mangkang, dan Karanganyar respectively. Bioaccumulation factor can be estimated from the ratio of these heavy metal element in the sample and the water. Result shows that the bioaccumulation factor for mercury at 3 villages (Mangunharjo, Mangkang dan Karanganyar) is 195.942, 238.961, 279.614 respectively.
THE GROWTH ANALYSIS OF Stichopus vastus (Echinodermata: Stichopodidae) IN KARIMUNJAWA WATERS Bambang Sulardiono; Slamet Budi Prayitno; Ign Boedi Hendrarto
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Stichopus vastus is one of sea cucumber resources utilize which has commercial economic value, locally known  as “gametes”. Because this species is continuously exploited, while the nature of life has not been known, one of them especially its growth.  Aspect of growth is an important parameter which is used as a basis for evaluating these resources, hence this resource is well managed and can be utilized in a sustainable manner. The study aims to: (1) estimates the growth characteristics mortality rate, recruitment and potential use of sea cucumber of  S.  vastus in Karimunjawa. The study is expected to give beneficial to the species of sea cucumber resource management strategy at  Karimunjawa waters, Jepara. The analysis of the growth parameters of S. vastus obtained values ​​of growth coeficient (K) of 0.55 year-1 and length infinity L ∞ value of 315.80 mm. Therefore the obtained values ​​of K tend to be close to zero value, it indicates the nature of growth tends to slow and with a relatively long life.  Life span of  S. vastus is 5.41 years, which can achieve body lengths of 283.06 to 296.91 mm, and the monthly growth rate ranged from 9.0 to 12.37 mm. The results of the analysis of mortality showed that S. vastus has value of total mortality (Z) of 0.98,  natural mortality (M) of 0.298, and catch mortality (F) of 0.682, with the rate of exploitation (E) of 0.6963. This suggests that the rate of exploitation is above the threshold standard set by the Government of 0.5 (BRKP, 2004). The analysis of the S. vastus the recruitment showed that the higest percentage recruit occurred in May-June is 17.16 - 18.33%. This is presumably due to a population increase of the spawning process in those months, although the value of the addition is not very significant.   It is suggested the need for regulations regarding: (a) the catchment season which is based on the tendency of the reproductive patterns of S. vastus, in Karimujawa, (b) restrictions on the size of the catch, (c) catch quotas, (d) the methods that are environmentally friendly catch, (e) permits the ship / boat used to catch sea cucumbers business. To support the success of regulation, it is necessary to strengthen community institutions through the management of sea cucumber resource-based society, with the Co-management approach.
Co-Authors Aninditia Sabdaningsih Bagus Rimbayu Ardie Bambang Sulardiono Boedi Hendrarto Diana Rachmawati Fitriadi, Ren Gina Saptiani Gina Saptiani Istiyanto Samidjan Johannes Hutabarat Nestin E.W. Ningrum Ocky Karna Radjasa Purnama, Muhammad Romi Novriadi Rudolf Muskananfola, Max S arjito Sarjito - Seto Windarto Soedibya, Petrus Hary Tjahja Subagiyo Subagiyo Supono . Suryanti Sutrisno Anggoro VIVI ENDAR HERAWATI YS Darmanto