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All Journal Abdimas Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Majalah Ilmiah MOMENTUM Bioeksperimen: Jurnal Penelitian Biologi Journal of Health (JoH) Jurnal Labora Medika Jurnal Biomedika SEMAR Techno Conference on Innovation in Health, Accounting, and Management Sciences Proceeding Bengawan :Jurnal Pengabdian Masyarakat Gema Kesehatan Medical Journal of Soeradji
Rahmat Budi Nugroho, Rahmat Budi
Program Studi D3 Analis Kesehatan Fakultas Ilmu Kesehatan, Universitas Setia Budi Surakarta
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PROFIL SENSITIVITAS ANTIBIOTIK TERHADAP ISOLAT BAKTERI SWAB LUKA PENDERITA DIABETES MELITUS: ANTIBIOTIC SUSCEPTIBILITY PROFILE OF BACTERIAL ISOLATES FROM WOUND SWABS Budi Nugroho, Rahmat; D. Andang Arif Wibawa; Muhammad Evy Prasetyanto
GEMA KESEHATAN Vol. 16 No. 1 (2024): Juni 2024
Publisher : POLTEKKES KEMENKES JAYAPURA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47539/gk.v16i1.448

Abstract

Luka adalah setiap cedera yang terjadi pada tubuh yang disebabkan oleh kecelakaan, benturan, kekerasan, atau pembedahan yang menyebabkan kerusakan pada membran kulit atau jaringan di bawahnya. Infeksi luka dapat disebabkan oleh beberapa jenis mikroorganisme termasuk bakteri diantaranya E.coli, Staphylococcus sp, Klebsiella, Proteus sp, Enterobacter sp, Streptococcus sp dan lain-lain. Uji sensitivitas antibiotik dilakukan untuk mengetahui pola resistensi bakteri dan menghindari penyebaran bakteri resisten. Penelitian ini bertujuan untuk mengetahui jenis bakteri yang ditemukan pada swab luka dan mengetahui apakah bakteri tersebut sensitif atau resisten terhadap antibiotik. Metode penelitian ini adalah penelitian eksperimental diagnostik dilaksanakan di Laboratorium Mikrobiologi Universitas Setia Budi Surakarta. Identifikasi bakteri dengan menggunakan uji biokimia dan alat VITEK  2 (bio Mérieux, USA). Ditemukan bakteri Gram positif yaitu Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactie dan Enterococcus faecalis. Sedangkan bakteri Gram negatif yaitu Escherichia coli, Klebsiella pneumoniae, dan Acinetobacter baumannii. Bakteri Gram positif yang ditemukan sensitif terhadap antibiotik vancomycin, tigecycline, linezolid dan resisten terhadap eritromycin, ciprofloxacin, clindamycin, ampicylin. Bakteri Gram negatif sensitif terhadap antibiotik meropenem. Tingkat resistensi mikroba terhadap berbagai jenis antibiotik cukup tinggi, sebagian besar isolat bakteri menunjukkan resistensi terhadap satu atau lebih antibiotik. Kata kunci: Infeksi, Sensitivitas, Swab luka   A wound is any injury to the body caused by an accident, impact, violence, or surgery that causes damage to the skin membrane or tissue underneath it. Infection of the wound can be caused by several types of microorganisms, including E.coli, Staphylococcus sp, Klebsiella, Proteus sp, Enterobacter sp, Streptococcus sp, and others. Antibiotic sensitivity tests are conducted to determine patterns of bacterial resistance and avoid the spread of resistant bacteria. The study aimed to identify the type of bacteria found in the wound swab and determine whether the bacteria were sensitive or resistant to antibiotics. This method was diagnostic experimental research at the Microbiology Laboratory of the University of Setia Budi Surakarta. They identified bacteria using biochemical tests and VITEK 2 (bio Mérieux, USA) devices. Gram-positive bacteria were found: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactia, and Enterococcus faecalis. Gram-negative bacteria include Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii. Gram-positive bacteria were found to be sensitive to the antibiotics vancomycin, tigecycline, and linezolid and resistant to erythromycin, ciprofloxacin, clindamycin, and ampicillin. Gram-negative bacteria are sensitive to antibiotic meropenem. The degree of microbial resistance to various antibiotics was relatively high, with most bacterial isolates showing resistance against one or more antibiotics. Keywords: Infection, Sensitivity, Swab Wound
UJI AKTIVITAS ANTI BAKTERI EKSTRAK ETANOLIK UMBI AKAR BATU (Coccinia grandis L.Voight) TERHADAP BAKTERI SALMONELLA SP Rukmana, Rizal Maarif; Nugroho, Rahmat Budi; Wisnumurti, Dwi Admani
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8390

Abstract

AbstractSalmonella sp are bacteria that often infect human and animal. This type of bacteria can cause Salmonellosis in human. Previous research has been carried out on secondary metabolites of plants as medicinal ingredients. One of the plants that has potential as a medicine is stone Akar Batu (Coccinia grandis L Voight). This study aims at examining the antibacterial activities of ethanolic extract of stone Akar Batu on Salmonella sp. The extraction method was carried out by maceration and the solvent used was ethanol 96%. Antibacterial activity was conducted using diffusion method. Identification of compound groups of extracts was done by reacting extracts with various chemical reagents. The results showed that stone of Akar Batu powder had a moisture content of 5.99% and a yield of 1.76%. The identification results of ethanolic extract of stone Akar Batu chemical group indicated that the extract contained flavonoids, alkaloids, saponins and tannins. The results of antibacterial activity test have revealed that ethanolic extract of stone Akar Batu can inhibit Salmonella sp.
Identifikasi dan Uji Sensitivitas Antibiotik pada Bakteri PenyebabInfeksi Saluran Kemih di RS Soeradji Tirtonegoro Klaten Wulandari, Retno; Nugroho, Rahmat Budi; Puspawati, Nony
MJS Medical Journal of Soeradji Vol 1 No 2 (2024): MJS (Desember)
Publisher : RSUP dr.Soeradji Tirtonegoro Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70605/wjt7e808

Abstract

Latar Belakang: Infeksi saluran kemih (ISK) adalah kondisi yang ditandai dengan adanya mikroorganisme patogen di dalam urin, kandung kemih, uretra, ginjal, atau prostat. Penyebab ISK adalah 95% karena bakteri, sementara 5% sisanya disebabkan oleh mikroorganisme lain. Gejala ISK meliputi keinginan untuk buang air kecil meskipun kandung kemih kosong, atau nyeri saat buang air kecil. Standar emas untuk penegakan diagnosis dan terapi ISK adalah melalui kultur urin Tujuan: Mengetahui jenis bakteri penyebab ISK dan pola sensitivitasnya terhadap antibiotik. Metode: Penelitian ini deskriptif dengan desain cross sectional. Data 30 sampel urin pasien ISK di RS Soeradji Tirtonegoro Klaten yang diperiksa untuk kultur dan sensitivitas terhadap antibiotik pada bulan Oktober-Desember 2023. Identifikasi bakteri menggunakan alat Matrix Assisted Laser Desorption Ionization Time Of Flight (MALDI TOF). Sensitivitas antibiotik menggunakan alat BD Phoenix, dengan interpretasi hasil sensitif, intermediet, atau resisten sesuai standar CLSI. Hasil: Dari 30 sampel urin, ditemukan 37 isolat bakteri. Bakteri penyebab ISK meliputi Escherichia coli (32,43%), Klebsiella pneumoniae (16,22%), dan Enterococcus faecalis (10,81%). Escherichia coli sensitif terhadap Amikasin (100%), Meropenem (70%), Imipenem (75%), dan Piperasilin/Tazobaktam (75%). Klebsiella pneumoniae sensitif terhadap Amikasin (100%), Imipenem (83%), Meropenem (67%), Gentamisin (67%), dan Piperasilin/Tazobaktam (67%). Enterococcus faecalis sensitif terhadap Ampisilin (100%), Linezolid (100%), Penisilin (75%), dan Vankomisin (75%). Kesimpulan: Bakteri penyebab ISK yang ditemukan pada urin pasien ISK adalah Escherichia coli (32,43%), Klebsiella pneumoniae (16,22%), Enterococcus faecalis (10,81%). Escherichia coli dan Klebsiella pneumoniae sensitif 100% terhadap Amikasin, sementara Enterococcus faecalis sensitif 100% terhadap Ampisilin dan Linezolid
Co-Authors Andang Arif Wibawa Arif Wibawa, S.P., M.Si., Dionysius D. Andang Arif Wibawa Dian Kresnadipayana Dwi Admani Wisnumurti Gregorius Indra Budianto Gregorius Indra Budianto, Gregorius Indra Gregorius Prima Indra Budianto Gregorius Prima Indra Budianto, Gregorius Prima Indra Happy Mulyani Happy Mulyani Ifandari Ifandari Iloh Antarini Kartinah Wiryosoenjoyo Kuntowati, Retno Lila Fadilla Masita, Tutus Elsa Muhammad Evy Prasetyanto Mukti, Patria Nony Puspawati Nur Segi Octavia Prilya Shanty Andrianie R.C. Hidayat Soesilohadi Retno Wulandari Rinda Binugraheni, Rinda Rizal Maarif Rukmana Rizal Maarif Rukmana Rizal Maarif Rukmana, Rizal Maarif Siti Umamah Susan Primadevi Susan Primadevi Susan Primadevi, Susan Wisnumurti, Dwi Admani