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All Journal Jurnal Teknologi Dan Industri Pangan VALENSI ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Sains Materi Indonesia Microbiology Indonesia Majalah Ilmiah Ekonomi Komputer Jurnal Penelitian Hasil Hutan Agrointek Majalah Kulit, Karet, dan Plastik Jurnal Industri Hasil Perkebunan Indonesian Journal of Chemical Science INDONESIAN JOURNAL OF ESSENTIAL OIL Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ilmu Kefarmasian Indonesia Jurnal Penelitian Hasil Hutan Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Pijar MIPA Makara Journal of Science JURNAL REKAYASA KIMIA & LINGKUNGAN
Tun Tedja Irawadi
Chemistry Department, Faculty Of Mathematics And Natural Science, IPB University, Kampus IPB Dramaga, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Journal : Microbiology Indonesia

 1 
Cyclo (Tyrosyl-Prolyl) Produced by Streptomyces sp.: Bioactivity and Molecular Structure Elucidation ROFIQ SUNARYANTO; BAMBANG MARWOTO; LIESBETINI HARTOTO; ZAINAL ALIM MAS'UD; TUN TEDJA IRAWADI
Microbiology Indonesia Vol. 5 No. 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.153 KB) | DOI: 10.5454/mi.5.2.5

Abstract

Determination of bioactivity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by Streptomyces sp. have been carried out. The antibiotic was produced by liquid culture using Streptomyces sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, 1H NMR, 13C NMR, and 13C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC27853 , Staphylococcus aureus ATCC25923, and Bacillus subtilis ATCC66923 were 27.0, 68.7, 80.2, and 73.7 μg mL-1 , respectively. Identification using ESI-MS showed that the molecular weight of this antibiotic was 260 g mol-1 , and molecular formula was C14H16N2O3 . Elucidation of molecular structure using 1HNMR, 13C NMR, and 13C DEPT NMRshowed that antibiotic was cyclo(tyrosyl-prolyl).
Selection of Carbon and Nitrogen Source for 8-Hydroxy 9, 12-Octadecadienoic Acid Production using Endophytic Fungi Curvularia lunata BioMCC FE-00283 ERWAHYUNI ENDANG PRABANDARI; TUN TEDJA IRAWADI; WAHONO SUMARYONO; KHASWAR SYAMSU
Microbiology Indonesia Vol. 5 No. 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.109 KB) | DOI: 10.5454/mi.5.4.6

Abstract

Hydroxyoctadecadienoic acid (HODE) is one of hydroxy fatty acids that has anticancer activity.  HODE was previously produced by chemical synthesis or bioconversion from linoleic acid. This is the first paper reported production of HODE by Curvularia lunata an endophytic fungi of Cibotium barometz. Various carbon and hydrogen sources have been tested for their effects on the production of  HODE by C. lunata. Glucose, lactose, maltose, xylose, and sucrose were used as carbon sources, while yeast extract, monosodium glutamate, urea, and NH4Cl were used as nitrogen sources. Fermentation was done using 100 ml medium in 250 ml Erlenmeyer flask at 150 rpm, 28 oC for 10 days.  HODE products were analyzed by high pressure liquid chromatography using C18 coloumn and eluted by gradient system of acetonitril-water from 15% to 100%. Glucose  and monosodium glutamate were found to be the best carbon and nitrogen source.  The optimum concentration of glucose and monosodium glutamate for the production of HODE were 10 mg L-1 and 12 mg L-1 respectively.
Co-Authors . Muntamah A.A. Ketut Agung Cahyawan W Adi Cifriadi Afifi, Muhammad Ridho Ahmad Zakaria Aini, Zahratul Ali, Mohamad Makrus Amatullah, Afra Najiyah Amril Aman Anas Miftah Fauzi Ani Suryani Awan Purnawan BAMBANG MARWOTO Bambang Marwoto Bambang Marwoto Irawadi Cifriadi, Adi Dewi Sondari Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi Setyaningsih ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI Henny Purwaningsih Inda Iliyin Irma Herawati Suprapto Jorion Romengga Jorion Romengga Kapelle, Imanuel B. D. Khaswar Syamsu Komar Sutriah Komar Sutriah Krisnawati Krisnawati Krisnawati, Krisnawati Liesbetini Hartoto LIESBETINI HARTOTO Liesbetini Hartoto M. Syamsul Maarif Mas’ud, Zainal Alim Meika Syahbana Rusli Mira Rivai Mohamad Rafi Mohammad Khotib Muhammad Agus Muljanto Muhammad Hanafi Muhammad Romli dan Suprihatin Andes Ismayana Noviyan Darmawan Pamungkas, Wahyu Widji Puspitasari, Santi Retno Djulaika Rofiq Sunaryanto ROFIQ SUNARYANTO Rofiq Sunaryanto Santi Puspitasari Silvester Tursiloadi Sr Sugiarti Sugara, Taufan H Suharto Honggokusumo Suprapto, Irma Herawati Tanto Pratondo Utomo Taufan H Sugara Tetty Kemala Wahono Sumaryono Widia Citra Anggundari, Widia Citra Yandra Arkeman YOPI YOPI Zainal Alim Mas'ud Zainal Alim Mas'ud Zainal Alim Mas'ud ZAINAL ALIM MAS'UD Zainal Alim Mas’ud Zainal Alim Mas’ud Zainal Alim Mas’ud Zainal Alim Mas’ud