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Charles Rangga Tabbu
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Journal : Jurnal Veteriner

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Tapak Perlekatan Reseptor Virus Flu Burung yang Diisolasi dari Berbagai Unggas Sejak tahun 2003 sampai 2008 (RECEPTOR BINDING SITE OF AVIAN INFLUENZA VIRUS H5N1 ISOLATED FROM VARIOUS POULTRIES SINCE 2003 TO 2008) Michael Haryadi Wibowo; Charles Rangga Tabbu; Widya Asmara; Heru Susetya
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian Influenza (AI) is an infectious disease in poultry, caused by type A of avian influenza virus(AIV), in the family of Orthomyxoviridae. Almost all birds’ species are sensitive to the AI. Beside theability to infect various species of poultry. AIV type A has a wide range of host including all bird species,mammals, dan human. Today some scientists reported that the cases of AI in mammals, including humansare increasing. This condition suggests that the AI virus circulated in the field may have some mutationsin the amino acid determinants responsible receptor binding site (RBS). A research was therefore designedto investigate the molecular level of HA gen fragment responsible for receptor binding site of AIV isolatedfrom various poultry since 2003 to 2008. Molecular characterization was based on the amplification ofreceptor binding site of HA gene by reverse transcriptase polymerase chain reaction (RT-PCR). All RTPCRof HA gene positive products were sequenced to determine the nucleotide composition at the targetedfragment. Sequences yielded were analyzed by program Mega 4.0 versions, including multiple alignment,deductive amino acid prediction, and establishment of phylogenetic tree. The results show that all AIVisolates could be determined of some conserved amino acids residues responsible for RBS which indicatethe binding preference of avian like receptor, sialic acid ? 2, 3 galactose except isolate A/Layer/Jabar/MHW-RBS-02/2008 which could be found a deletion of amino acid at position of 129 dan mutation of 151isoleucine into threonine. Phylogenetic study showed that clustering of AIV did not base on species of birdor geographic origin of AI viruses which were studied.
Kajian Molekuler Daerah D-Loop Parsial DNA Mitokondria Kuda (Equus caballus) Asli Tengger Yuriadi -; Rini Widayanti; Aris Purwantoro; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Tengger’s horse (Equus caballus) is a local Indonesian horse an originated from its ancestor in Java.As the population of Tengger’s horse is almost extinct it is important to conserve and increase the horsepopulation by in situ or ex situ conservation.The objective of this research was to study the moleculargenetic of partial D-loop of Tengger’s horse. Sequencing of PCR product, showed that the D-loop consistedof 319 nucleotides. The DNA was isolated from whole blood and amplified and sequenced using a publishedprimer sets. The sequence was aligned and compared with horse D-loop sequences available in Genebankusing Clustal W method in MEGA program version 4.0.2. Ten different nucleotide sites were found inTengger horse from (nucleotide no. 9, 52, 64, 69, 102, 117, 133, 170, 187 and 293). The genetic distanceanalised using Kimura 2-parameter model ranged between 0,0% and 3,2%, with the average of 1,7%. Thephylogenetic tree using neighbor joining method based on the sequence of nucleotide partial D-loop couldnot be used to differentiate among horse from Tengger and E. caballus.
AMINO-TERMINUS OF POLYMERASE BASIC-2 OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM VARIOUS ANIMAL SPECIES IN INDONESIA Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The information on pathogenicity and adaptation factors of avian influenza virus (AIV) in mammalsis very inportant in an effort to reduce the risk of avian influenza (AI) pandemic in the future. Polymerasegene complex appears to be the major factors for adaptation of AIV to certain animal species. A preliminarystudy on role of non-coding region (NCR) and amino-terminus of polymerase-basic 2 (PB2) is presented.Purified viral RNA of AIV isolated from chicken, duck, pig, and quail of Bali and Yogyakarta was reversetranscribed into cDNA and amplified using reverse transcriptase-polymerase chain reaction (RT-PCR)using PB-2 universal forward primer and specifically designed backward primer. The result showed thatall AIV’s H5N1 isolated from chicken, duck, quail, and pig, posed PB2 amino-terminus typical for IndonesianAIV H5N1. However, polymorphic amino acids of the protein fragment did not show any species specificmotive, with the exception of the pig isolate Sw/Tabanan/2006 which had specific substitution of D16E,H17Q, M40I, and H124Y.
Virgin Coconut Oil Meningkatkan Aktivitas Fagositosis Makrofag Ayam Pedaging Pascavaksinasi Flu Burung (VIRGIN COCONUT OIL INCREASES THE PHAGOCYTOSIS ACTIVITY OF MACROPHAGE OF BROILER CHICKEN FOLLOWING AVIAN INFLUENZA VACCINATION) Enny Yusuf Wachidah Yuniwarti; Widya Asmara; Wayan Tunas Artama; Charles Rangga Tabbu
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The research objective was to find an alternative avian influenza prevention in broilers by increasinganimal’s antibody titer and macrophages phagocytic  activity.  Virgin coconut oil (VCO) is a food supplementthat is proven safe for human consumption therefore it is assumed to be safe for the animal’s (chickens).Factorial design  2 vaccinated: unvaccinated) x 4 (dose of VCO: 0, 5, 10 and 15 mL/kg feed) were applied inthis study.  A total of 40 day day old chick were allocated in the eight treatments groups.  Feed and drinkingwater were available  ad libitum.  Antibody titers of the animals were detected using ELISA, whereasphagocytic activity of the macrophages were detected from spleen.  The result showed that the highestphagocytic activity and antibody titers were seen in chickens which were given VCO at 10 mL/kg feed.  It isconcluded that the VCO could increased the phagocytic activity of macrophages.
Penerapan Metode Diagnosis Cepat Virus Avian Influenza H5N1 dengan Metode Single Step Multiplex RT-PCR Aris Haryanto; Ratna Ermawati; Medania Purwaningrum; Dini Wahyu Yudianingtyas; Michael Haryadi Wibowo; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian influenza (AI) virus is a segmented single stranded (ss) RNA virus with negative polarity andbelong to the Orthomyxoviridae family. Diagnose of AI virus can be performed using conventional methodsbut it has low sensitivity and specificity. The objective of the research was to apply rapid, precise, andaccurate diagnostic method for AI virus and also to determine its type and subtype based on the SingleStep Multiplex Reverse Transcriptase-Polymerase Chain Reaction targeting M, H5, and N1 genes. In thismethod M, H5 and NI genes were simultaneously amplified in one PCR tube. The steps of this researchconsist of collecting viral RNAs from 10 different AI samples originated from Maros Disease InvestigationCenter during 2007. DNA Amplification was conducted by Simplex RT-PCR using M primer set. Then, bysingle step multiplex RT-PCR were conducted simultaneously using M, H5 and N1 primers set. The RTPCRproducts were then separated on 1.5% agarose gel, stained by ethidum bromide and visualized underUV transilluminator. Results showed that 8 of 10 RNA virus samples could be amplified by Simplex RTPCRfor M gene which generating a DNA fragment of 276 bp. Amplification using multiplex RT-PCRmethod showed two of 10 samples were AI positive using multiplex RT-PCR, three DNA fragments weregenerated consisting of 276 bp for M gene, 189 bp for H5 gene, and 131 bp for N1. In this study, rapid andeffective diagnosis method for AI virus can be conducted by using simultaneous Single Step Multiplex RTPCR.By this technique type and subtype of AI virus, can also be determined, especially H5N1.
Akumulasi Timbal dalam Cakar Ayam Kampung Djohan -; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Domestic chickens tend to consume lead (Pb) from their contaminated environment especially in freerangingchickens. Feet of domestic chickens are commonly consumed by many people, making them goodbioindicator to be analyzed for environmental monitoring and food safety purposes. Accumulation of leadin feet and parts of feet (tarsometatarsus bones, digiti, and skin-muscles) were investigated in this study.The average concentrations of lead in whole feet, tarsometatarsal bones, digiti, and skin-muscles were 3.4,3.8, 3.3, and 1.9 ?g.g-1 d.m., respectively. The average amount of Pb accumulated in chicken feet ranges from25.5 to 74.7 ?g. Consumption of 0.5 – 2 chicken feet. A day-1 (low – high intake) results in the exposure of 4,8to 111,5 ?g. individual-1.day-1, which was much higher than the daily exposure standar of 1 ?g. individual-1. day-1. However, very low intake (< 0.1 chicken feet.day-1) results in exposure lower than the recommendedexposure standard. More frequent monitoring and exposure assessment combined with the awareness ofgeneral public on lead contamination in the environment are important for minimizing the risk of leadexposure to human through chicken feet consumption.
Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Yuli Purwandari Kristianingrum; Charles Rangga Tabbu; Bambang Sutrisno; Sitarina Widyarini; Kurniasih .; Tri Untari; Asmarani Kusumawati
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.
Non Coding Region dan Amino Terminus Gen Polimerase Asidik Virus Avian Influenza Subtipe H5N1 Asal Hewan Indonesia Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The knowledge on the species adaptation factor of avian influenza virus of H5N1 subtype (AIV H5N1)is very important as a signal for the emergence of a new strain with pandemic potential. This research wasconducted to find out the sequence variation of the Non-Coding Region (NCR) and Coding Region (CR) of 5’-terminal cRNA of the polymerase acidic (PA). Total RNA from twenty six (26) avian influenza virussubtype H5N1 isolates were amplified using reverse-transcriptase-polymerase chain reaction (RT-PCR)with a universal forward primer for influenza virus and specifically designed backward primers. Nineteen(19) gene fragments of PA could be amplified. RT-PCR products were sequenced and analyzed using Mega4 software. The length of NCR of PA gene was found to be 24 bases and conserved. A/T composition of PANCR was 58.3%. Species and geographical specificity could not be found in the genetic distance, the aminoacid polymorphism, as well as the phylogenetic analysis of the CR. RNA sequencing is discussed andrecomended to be studied further.
VARIATION OF NON-CODING REGION AND CODING REGION OF 5’-TERMINAL CRNA OF POLYMERASE BASIC 1 OF AVIAN INFLUENZA VIRUS SUBTYPE H5N1 Gusti Ayu Yuniati Kencana; Widya Asmara; Charles Rangga Tabbu; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The sequence of the Non-Coding Region (NCR) and Coding Region (CR) of 5’-terminal cRNA of thepolymerase basic 1 (PB1) gene as a major factor for the species adaptation of avian influenza virussubtype H5N1 (AIV H5N1) has been analysed. The information could be a virological signal for theemergence of a new strain with pandemic potential. Total RNA from twenty six (26) avian influenzasubtype H5N1 isolates were amplified using reverse-transcriptase-polymerase chain reaction (RT-PCR)with a universal forward primer for influenza virus and specifically designed backward primers. Fifteen(15) PB1 gene fragments could be amplified. RT-PCR products were sequenced and analyzed using Mega4software. The length of NCR of PB1 gene was found to be 24 bases and mostly shows conserved sequence,with an exception of Dk/Badung/2006 isolate which has C-7T substitution. A/T composition of PB1 NCRwas 54,2%, while the Dk/Badung/2006 isolate was 58,3%. Species and geographical specificity could not befound in the genetic distance, the amino acid polymorphism, as well as the phylogenetic analysis of t
Akumulasi Timah Hitam dalam Daging dan Tulang Ayam Kampung dan Ayam Negeri (LEAD ACCUMULATION IN MEAT AND BONES OF DOMESTIC AND BROILER CHICKEN) Djohan .; Charles Rangga Tabbu
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Lead is a heavy metal polluting the environment, and its accumulation in animal or human bodies canhave neurotoxic and nephrotoxic effects on animals and human. Lead-contaminated chicken meat can bethe source of lead to human. Lead exposure to human can be assessed by measuring its concentration andaccumulation in chicken body parts and analyzing chicken consumption patterns. This study was conductedto measure lead concentrations in chicken body parts and to estimate lead exposure caused by consumptionof chicken body parts (breast, legs, wings) and tissues (meat, skin, cartilage, spongy bones). Samples wereextracted by using aqua regia digestible method with a mixture of HCl: HNO3 (3:1; v/v) and leadconcentrations were measured by the Atomic Absorption Spectrophotometry (AAS) method. The leadconcentrations in chicken tissues varied from< 0.01 to 1.81?g.g-1dry weight.The average concentrations oflead in chicken tissues were lower than the recommended safety level of lead in chicken meat (1.0?g.g-1),except for the breast cartilage (1.03?g.g-1). The lowet accumulation level 2.6 ?g g-1 was found in domesticchicken wings while the highest of 32.9 ?g g-1 was found in broiler chicken breast (total of meat, skin,cartilage). Based on the data of lead accumulation in chicken tissues, a polynomial equation describing theprobability (P) to be exposed to certain amount of lead in chicken tissues (A, in ?g) was determined as P =-(1 x 10-3)A2 + (6,4 x 10-2)A.
Co-Authors Agnesia Endang Tri Hastuti Wahyuni Agus Eko Srihanto Aris Haryanto Aris Purwantoro Asmarani Kusumawati Bambang Sumiarto Bambang Sutrisno Dini Wahyu Yudianingtyas Djohan - Djohan . Dyah Ayu Widiasih Enny Yusuf Wachidah Yuniwarti Gusti Ayu Yuniati Kencana Haryadi M. Wibowo Heru Susetya Heru Susetyo I Gusti Ngurah Kade Mahardika Khrisdiana Putri Kurniasih . M. Haryadi Wibowo Medania Purwaningrum Michael Haryadi Wibowo Ratna Ermawati Rini Widayanti Setyawan Budiharta Sidna Artanto Sitarina Widyarini Tati Aryani Tri Untari Vinsa Cantya Prakasita Wayan Tunas Artama Widya Asmara Yuli Purwandari Kristiangingrum Yuriadi -