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All Journal International Journal of Public Health Science (IJPHS) Jurnal Kesehatan Lingkungan indonesia Jurnal Ilmu Dasar Jurnal Natur Indonesia e-Journal Pustaka Kesehatan Jurnal Kesehatan Masyarakat Indonesian Journal of Tropical and Infectious Disease Journal of Agromedicine and Medical Sciences Elkawnie The Indonesian Journal of Occupational Safety and Health Majalah Kedokteran Sriwijaya Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) Medical Laboratory Technology Journal Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan SRIWIJAYA JOURNAL OF MEDICINE Jurnal Respirologi Indonesia Jurnal Kesehatan Journal of Health Sciences Berkala Penelitian Hayati JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery)

Diana Chusna Mufida

Departement Mikrobiologi, Fakultas Kedokteran, Universitas Jember, Jl. Kalimantan No.37, Kampus Tegal Boto, Kabupaten Jember, Jawa Timur, 68121

Author-ID : 247760

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health

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The 35,2 kda Hemaglutinin Protein of Pili’s Proteus mirabilis P355 as Adhesin on Rabbit’s Blader Epitelial Diana Chusna Mufida; Enny Suswati
Jurnal ILMU DASAR Vol 8 No 1 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.208 KB)

Urinary tract infection represent one of nosocomial infection in hospital. One of agent nosocomial urinary tract infection is Proteus mirabilis bacteria and common occur on patient with urinary catheter. Urinary tract infection with caused by P. mirabilis was persistent, very difficult to eradicated. Further more is caused some complication such as cystitis, acut and cronic pyelonephritis, kidney bladder stone, bacterimiae and sepsis. This bacteriae has same virulence factors. Fimbriae is one of it. In the adhesion test utilization of protein hemaglutinin pili 35.2 kDa resulted in the electroelusion which salluted in vesica urinaria epitelial by dose 400μl , 200μl, 100μl, 50μl, 25μl, 12,5μl and 0μl as control. Form adhesion test on vesica urinary epitelial that salluted with 35.2kDa protein pili we faund that the higher dose of protein can decrease bacteriae consentration at vesica urinary epitelial. This result was significant with r= 0.93 and p value = 0.005. This conclusion of this study is 35.2 kDa molecular weight protein pili of P. mirabilis P355 were adhesion protein.Keywords : hemaglutinin, pili, Proteus mirabilis, adhesin

The Role of Pili Protein 38,6 kDa Klebsiella pneumoniae as a Hemagglutinin and Adhesin Protein which Serves as a Virulence Factor Regina Finka; Dini Agustina; Dwita Aryadina Rachmawati; Enny Suswati; Diana Chusna Mufida; Ali Shodikin
Journal of Agromedicine and Medical Sciences Vol 5 No 2 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i2.9558

Klebsiella pneumoniae is an infectious bacteria in various parts of the body. The presence of proteins in pili that will bind to cell surface receptors (adhesin proteins) and cell membrane sugar molecules (hemagglutinin proteins) is a very influential factor in the ability of K. pneumoniae to enter the human body. The purpose of this study was to determine the role of pili 38.6 kDa K. pneumoniae protein as a hemagglutinin and adhesin protein which functions as a virulence factor. This type of research is pure experimental research using the electrophoresis method (SDS-PAGE) to obtain protein. The protein obtained was then tested for hemagglutination and adhesion test using erythrocyte cells and enterocytes of BALB / C mice to determine their role as hemagglutinin and adhesin proteins. Conclusion of this study pili protein 38.6 kDa Klebsiella pneumoniae acts as a hemagglutinin and adhesion protein which functions as a virulence factor. Keywords: Klebsiella pneumonia, 38,6 kDa Protein, Hemagglutinin, Adhesin

The Morphological Changes of Staphylococcus Aureus Caused by Ethanol extracts of Cocoa Beans (Theobama Cacao) through In Vitro Dafista Diyantika; Diana Chusna Mufida; Misnawi Misnawi
Journal of Agromedicine and Medical Sciences Vol 3 No 1 (2017)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v3i1.4094

Staphylococcus aureus (S. aureus) is positive gram bacteria which often infect humans. The use of antibiotic for therapy is the factor of resistance. S. aureus resistant towards some biotic like tetracycline, streptomycin, chloramphenicol, ampicilin, eritromisin and penicillin. The increase of the resistance demands more findings on new drugs. Ethanol extracts of cocoa beans has a block capacity towards the growth of S. aureus via in vitro. The effect of antibacterial of ethanol extracts has already been proved. However, its morphology changes on S. aureus after given ethanol extracts of cocoa beans is not found yet. This research is aimed at studying the morphological changes on S. aureus after given ethanol extracts of cocoa beans. The research design is Quasi Experimental with Posttest Only Control Group Design. The sample used is S. aureus whereas the material tests is ethanol extracts of cocoa beans in concentration of 31.2 mg/mL; 15.6 mg/mL and 7.8 mg/mL. The negative control is sterile aquades and the positive control is cephalexin suspension 4 ug/mL. The method for observing the S. aureus morphology change is scanning electronic microscope. The research result shows that there is a morphology change. Given ethanol extracts of cacao seed at 7,8 mg/mL and 15,6 mg/mL, there is enlargement of wall cell and the unity of septum. And at 31,25 mg/mL, there is protrusion at the wall cell and enlargement of wall cell.Keywords: Antimikrobial, Cacao, SEM, Staphylococcus aureus.

Antibiotic Sensitivity Test on Staphylococcus Aureus Detected in Sputum of Patients with Pneumonia Treated in Hospitals Dini Agustina; Diana Chusna Mufida; Hanifa Rizki A.S.; Dion Krismashogi Dharmawan
Journal of Agromedicine and Medical Sciences Vol 5 No 1 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i1.9267

Pneumonia is a respiratory tract infection that attacks the pulmonary parenchyma. This disease can be caused by bacteria, one of which is Staphylococcus aureus. Antibiotics have an important role to play in reducing the morbidity and mortality incidence of pneumonia, but currently, the incidence of resistance antibiotics is increased. The purpose of this study was to determine the sensitivity of antibiotics Staphylococcus aureus in pneumatic sputum patients. The design of this study was observational descriptive, using sputum samples from pneumonia patients in Subandi and Paru Jember hospitals in November to December 2018. The identification of bacteria in sputum was continued by testing the sensitivity of bacteria to antibiotics. The antibiotics used in this study were chloramphenicol, gentamicin, amikacin, levofloxacin, ampicillin-sulbactam, cotrimoxazole, and ciprofloxacin — data obtained in the frequency distribution table. Of the ten sputum that was successfully cultured, four Gram-negative bacilli bacteria and six Gram-positive coccus bacteria were obtained. The results approved the six Gram-positive coccus bacteria, detected as Staphylococcus aureus. The antibiotic resistance of Staphylococcus aureus to ampicillin-sulbactam was high. Keywords: antibiotic sensitivity, bacteria, pneumonia

PERAN PROTEIN MEMBRAN LUAR 55 kDa Salmonella typhi ISOLAT JEMBER SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Diana Chusna Mufida; Candra Bumi; Heni Fatmawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009
Publisher : The East Java Biological Society

Salmonella typhi is an obligate pathogen that is usually found in clinical specimen from typhoid fever patients. The pathogenic mechanism of bacteria are not fully elucidated especially its potential activity of the outer membrane protein (OMP) as hemaglutinin and adhesion molecule. After identification, bacterial isolate of outer membrane protein fraction 12,5% SDS-PAGE were used to isolate OMP followed by hemaglutinin test and in vitro adhesion test. The study showed that the 55 kDa protein of S. typhi is a hemaglutinin protein that could agglutinate, Wistar mouse erythrocytes. The 55 kDa OMP is also an adhesion protein showed by its activity to adherence to Wistar mice’s enterocyt. The increased dose of 55 kDa OMP will decrease the amount of S. typhi bacteria adherenced to Wistar mice’s enterocyt. Outer membrane protein 55 kDa S. typhi as hemagglutinin and also adhesion protein.

Correlation Between Leukocyte Differential Counts with The Severity and Outcome of Coronavirus Disease 2019 (COVID-19) Patients In Jember Angga Mardro Raharjo; Eprila Darma Sari; Diana Chusna Mufida
Jurnal Respirologi Indonesia Vol 41, No 3 (2021)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v41i3.186

Background: Coronavirus Disease 2019 (COVID-19) is an acute respiratory disease caused by new strain of RNA viruses named Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Hematological changes, especially leukocyte differential counts, presumed to be a predictor of the severity and outcome of COVID-19 patients. This study aimed to analyze the correlation between leukocyte differential counts with the severity and outcome of COVID-19 patients. Methods: This study was conducted using a cross-sectional analytic observational method, through secondary data analysis of COVID-19 patients who were tested positive by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and hospitalized between April–November 2020 at Citra Husada Jember, Kaliwates Jember, and Jember Klinik Hospital. Results: From 267 patients, there were 139 female patients (52.1%) and 128 male patients (47.9%). There was a positive correlation on leukocytes, neutrophil, and neutrophil-lymphocyte ratio (NLR) with the severity and outcome of the patients, and negative correlation on eosinophils, lymphocytes, and monocytes (P 0.05) Conclusion: Leukocyte differential counts examination could be a predictor of the severity and outcome of COVID-19 patients, especially neutrophils, lymphocytes, and NLR.

Peran Protein Hemaglutinin Pili Streptococcus Pneumoniae 54 Kda Sebagai Adhesin Diana Chusna Mufida; Adellia Fira Fa'idha; Zahrah Febianti
Jurnal Ilmiah Kesehatan Vol 13 No 2 (2020): Jurnal Ilmiah Kesehatan (Journal of Health Science)
Publisher : Universitas Nahdlatul Ulama Surabaya

Pili memiliki protein adhesin yang berperan dalam proses adhesi. Penelitian menunjukkan bahwa protein pili S. pneumoniae 54 kDa merupakan protein hemaglutinin. Tujuan penelitian ini adalah menguji peran protein hemaglutinin pili S. pneumoniae 54 kDa sebagai adhesin. Pili S. pneumoniae diisolasi menggunakan alat pili cutter. Hasil potongan pili dilakukan SDS-PAGE dan diidentifikasi berat molekul proteinnya. Protein berat molekul 54 kDa diisolasi sehingga menghasilkan protein larutan. Dilakukan uji adhesi pada larutan protein dengan konsentrasi bertingkat untuk mengetahui indeks ahesi. Uji korelasi Pearson diperoleh nilai p-value 0,036 (p < 0,05) dan koefisien korelasi R= -0,840, hasil ini menunjukkan bahwa kedua variabel memiliki hubungan yang signifikan, sangat kuat dengan arah hubungan negatif. Analisis regresi didapatkan R2 0,997, artinya 99,7 % konsentrasi protein pili 54 kDa S. pneumoniae mempengaruhi indeks adhesi, sehingga dapat disimpulkan protein hemaglutinin pili 54 kDa S. pneumoniae merupakan protein adhesin.

Hubungan Kejadian Ko-Infeksi Soil-Transmitted Helminths pada Pasien Tuberkulosis dengan Jumlah Eosinofil di Kecamatan Kalisat Kabupaten Jember Widhiasari Normaningtyas; Muhammad Ali Shodikin; Angga Mardro Raharjo; Dini Agustina; Diana Chusna Mufida; Yunita Armiyanti
Jurnal Ilmiah Kesehatan Vol 13 No 2 (2020): Jurnal Ilmiah Kesehatan (Journal of Health Science)
Publisher : Universitas Nahdlatul Ulama Surabaya

Abstrak: Tuberkulosis (TB) menjadi masalah kesehatan utama baik lingkup global maupun nasional. Tuberkulosis menyebabkan status gizi kurang baik. Status gizi yang kurang baik dapat disebabkan oleh infeksi kronis, salah satunya infeksi cacing usus terutama Soil-Transmitted Helminths (STH). Ko-infeksi STH pada pasien TB selain mempengaruhi status gizi, juga akan mempengaruhi respon imun pasien. Respon imun pada pasien ko-infeksi STH akan menghambat respon imun Th 1 dalam melawan TB serta akan didapatkan peningkatan eosinofil (eosinofilia). Tujuan utama dalam penelitian ini adalah untuk mengetahui jumlah eosinofil pada pasien tuberkulosis terhadap ko-infeksi STH di Kecamatan Kalisat, Kabupaten Jember. Jenis penelitian ini adalah penelitian observasional dengan desain analitik cross sectional. Populasi yang digunakan adalah pasien TB di Kecamatan Kalisat dalam periode waktu September – Desember 2019. Data yang digunakan dalam penelitian ini adalah data primer berupa hasil pemeriksaan darah dengan metode hitung jenis dan hasil pemeriksaan feses menggunakan metode sedimentasi dan floatasi. Analisis data dilakukan menggunakan uji Fisher. Hasil penelitian menunjukkan bahwa kejadian koinfeksi STH pada pasien TB di Kecamatan Kalisat Kabupaten Jember sebesar 3,6% dan disebabkan oleh Hookworm. Hasil uji Fisher menunjukkan tidak ada hubungan yang signifikan antara jumlah eosinofil dengan kejadian koinfeksi STH pada pasien TB di Kecamatan Kalisat Kabupaten Jember dengan nilai p-value 1,000. Kata kunci: Tuberkulosis, Soil-Transmitted Helminths, Eosinofilia

Bacterial and parasitic contamination of raw vegetable in Jember regency, Indonesia : potential risk for food-borne diseases Diana Chusna Mufida; Elvia Rahmi Marga Putri; Dini Agustina; Enny Suswati; Muhammad Ali Shodikin; Yunita Armiyanti; Bagus Hermansyah; Wiwien Sugih Utami; Angga Mardro Raharjo
International Journal of Public Health Science (IJPHS) Vol 11, No 4: December 2022
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v11i4.21875

A raw vegetable is one of the transmission factors in human food-borne infection. Contamination might start from the plantation, distribution, until at serving time in the dining table. A higher level of contamination will increase the risk of food-borne illness. The study aims to determine bacteria and parasite contamination in some raw vegetables that people usually consume in Jember Regency, East Java, Indonesia. In this study, we collected raw vegetable from eight traditional markets. We chose randomly at each market five samples of vegetables that usually directly consumed, like lettuce, tomato, cabbage, basil, long bean, and cucumber. Pathogens we identified limited on bacteria and parasite like helminth and protozoan group. This study showed that 91% contaminated by E. coli, 84% by Staphylococcus aureus, and 79% contaminated by Vibrio cholerae. Besides bacteria, 36% of samples contaminated by the helminth group, and protozoa contaminated 27%. The most vegetables contaminated was lettuce, even in bacterial or parasitic contamination. This study concluded that there is contamination in raw vegetables usually directly consumed in Jember Regency. It will be a potential risk factor for food-borne illness in the future.

Hubungan Pengetahuan Keamanan Pangan dengan Higiene Penjual dan Kontaminasi Salmonella spp Pada Lalapan Mentah di Kecamatan Patrang Adilah Julinar Irianti; Diana Chusna Mufida; Muhammad Ali Shodikin; Yudha Nurdian; Bagus Hermansyah; Angga Mardro Raharjo
Jurnal Kesehatan Lingkungan Indonesia Vol 21, No 2 (2022): Juni 2022
Publisher : Master Program of Environmental Health, Faculty of Public Health, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkli.21.2.180-187

Latar belakang: Berdasarkan data Wilayah Kerja Puskesmas Patrang didapatkan sebanyak 2964 kasus diare pada tahun 2020 di Kabupaten Jember. Diare dapat terjadi akibat infeksi bakteri Salmonella spp. yang menular secara oral fecal. Bakteri Salmonella spp seringkali mencemari bahan pangan terutama sayuran. Sementara itu, masyarakat Indonesia sering mengonsumsi sayuran mentah yang disebut dengan lalapan mentah. Kontaminasi bakteri pada lalapan mentah dapat disebabkan oleh kondisi higiene penjual yang kurang baik yang dipengaruhi oleh beberapa faktor, seperti pengetahuan keamanan pangan penjual. Penelitian ini bertujuan untuk mengetahui hubungan pengetahuan keamanan pangan dengan higiene penjual dan kontaminan Salmonella spp pada lalapan mentah di Kecamatan Patrang.Metode: Penelitian ini menggunakan metode observasional analitik dengan desain penelitian cross sectional, dan data dianalisis menggunakan uji Spearman Rank. Sampel ditentukan dengan teknik simple random sampling dengan jumlah sampel 30 penjual.Hasil: Hasil penelitian menemukan bahwa 83,3% penjual memiliki pengetahuan keamanan pangan yang baik, 46,7% penjual memiliki higiene personal yang cukup, dan 80% lalapan mentah terkontaminasi Salmonella spp. Pada uji analisis Spearman Rank tidak didapatkan hasil yang signifikan antara pengetahuan keamanan pangan dengan higiene penjual (0,721>0,05) dan tidak ada hasil yang signifikan antara pengetahuan keamanan pangan dengan Salmonella spp (0,235>0,05).Simpulan: Dapat disimpulkan bahwa tidak ada hubungan pengetahuan keamanan pangan dengan higiene penjual dan Salmonella spp pada lalapan mentah di Kecamatan Patrang. ABSTRACT Title: Relation of Food Safety Knowledge, Seller Hygiene, and Salmonella spp on Raw Lalapan in Patrang DistrictBackground: Based on data from the Patrang Health Center Work Area, there were 2964 cases of diarrhea in 2020 in Jember Regency. Diarrhea can occur due to infection with Salmonella spp. which is transmitted oral fecal. Salmonella spp can contaminate food especially vegetables. Meanwhile, Indonesian people often consume vegetables salad called raw lalapan. Raw lalapan contamination can caused by seller poor hygiene that influenced by several factors, such as their food safety knowledge. This study aims to determine the relation of food safety knowledge with seller hygiene and Salmonella spp contaminants on raw lalapan in Patrang District.Method: This study used an analytical observational method with a cross sectional study design, and the data were analyzed using Spearman Rank test. The sample was determined by a simple random sampling technique with a sample of 30 sellers.Result: The study found that 83.3% seller had good food safety knowledge, 46.7% seller had sufficient personal hygiene, and 80% of raw lalapan were contaminated with Salmonella spp. In the Spearman Rank analysis test, there were no significant results between food safety knowledge and seller hygiene (0.721>0.05) and no significant results between food safety knowledge and Salmonella spp contaminants (0.235>0 ,05).Conclusion: So, it can be concluded that there was no relation of food safety knowledge with seller hygiene and Salmonella spp contaminants in raw vegetables in Patrang District.

Co-Authors Adellia Fira Fa'idha Adilah Julinar Irianti Alfianti, Nita Angga Mardro Raharjo Angga Mardro Raharjo, Angga Mardro Azham Purwandhono Bagus Hermansyah Candra Bumi Dafista Diyantika Dafista Diyantika Danang Tejamukti Widiatmaja Dini Agustina Dion Krismashogi Dharmawan Dwita Aryadina Rachmawati Ellen Ocktavironita Elvia Rahmi Marga Putri Enny Suswanti ENNY SUSWATI Eprila Darma Sari Fitri, Nadya Eka Hanifa Rizki A.S. Heni Fatmawati Himma, Alyatul Iftinan, Karenzha Ika Rahmawati Sutejo Kristianningrum Dian Sofiana Lailatis Shofia M. Ali Shodikin Mei Syafriadi Misnawi Misnawi Misnawi Misnawi Nita Alfianti Nugraha, Bima Setia Sandya Nurul Indah Saffanah Pralampita, Pulong Wijang Putra Uta, Diego Rafi Putu Ayu Laksmi Lestari Qintar Layallia Faza Regina Finka S.Pd. M Kes I Ketut Sudiana . Sa'adah, Ajeng Samrotu Salsabila, Yuna Annisa Saputra, Antonius Dwi Shodikin, M. Ali Shofia, Lailatis Siti Marissa Aisyah Syahputri, Siti Ananda Hardita Wahyuningsih, dr. Rita Widhiasari Normaningtyas Widiatmaja, Danang Tejamukti Wiwien Sugih Utami Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Zahrah Febianti

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