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All Journal International Journal of Public Health Science (IJPHS) Jurnal Kesehatan Lingkungan indonesia Jurnal Ilmu Dasar Jurnal Natur Indonesia e-Journal Pustaka Kesehatan Jurnal Kesehatan Masyarakat Indonesian Journal of Tropical and Infectious Disease Journal of Agromedicine and Medical Sciences Elkawnie The Indonesian Journal of Occupational Safety and Health Majalah Kedokteran Sriwijaya Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) Medical Laboratory Technology Journal Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan SRIWIJAYA JOURNAL OF MEDICINE Jurnal Respirologi Indonesia Jurnal Kesehatan Journal of Health Sciences Berkala Penelitian Hayati JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery)
Diana Chusna Mufida
Departement Mikrobiologi, Fakultas Kedokteran, Universitas Jember, Jl. Kalimantan No.37, Kampus Tegal Boto, Kabupaten Jember, Jawa Timur, 68121
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health

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The Correlation between Personal Hygiene of Greengrocer and Coliform Bacteria Contamination in Lettuce in Jember Traditional Markets Qintar Layallia Faza; Diana Chusna Mufida; Muhammad Ali Shodikin; Bagus Hermansyah; Elvia Rahmi Marga Putri
Journal of Agromedicine and Medical Sciences Vol 8 No 2 (2022)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v8i2.30237

Abstract

Lettuce is a vegetable that is usually consumed in raw condition or unprocessed by people. Unprocessed vegetables can cause food-borne diseases. Lettuce that is not processed and being consumed directly can cause contamination by Coliform bacteria. The purpose of this study is to know the correlation between Greengrocer’s personal hygiene and Coliform bacteria contamination in lettuce in Jembertraditional markets. This research used a descriptive observational design with the cross-sectional approach. It took place in eight traditional markets in Jember Regency and Microbiology Laboratory, Faculty of Medicine, University of Jember. The samples used were 41 lettuces purchased from 41 traditional market sellers. The microbiological examination was performed in the laboratory using the most probable number method. The results showed that lettuce sellers had bad personal hygiene (92,7%) and the data from the most probable number test found 97,6% contaminated by Coliform bacteria. The bivariate analysis was used to know the relationship between personal hygiene vegetable seller and contamination Coliform bacteria in lettuce. Thep-value was 0,072, which means there was no relationship between personal hygiene vegetable seller and contamination Coliform bacteria in lettuce. Keywords: lettuce, Coliform, contamination, personal hygiene
Characterization of pili protein with molecular mass of 85 kDa Escherichia coli as an adhesin and a hemagglutinin Dini Agustina; Siti Marissa Aisyah; Ika Rahmawati Sutejo; Diana Chusna Mufida
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art5

Abstract

Background: Escherichia coli (E. coli) is a rod-shaped gram-negative bacterium included in Multi Drug Resistant (MDR) bacteria. There are several strategies in place to prevent and treat E. coli bacterial infections to reduce some the incidences of MDR. One of them is the development of a protein-based vaccine. Pili is one of virulence factors in E. coli surface proteins that can mediate attachments of bacteria to host cells (adhesin or hemagglutinin) and can be used as protein-based vaccine candidates. Objective: This study aims to determine characterization of pili protein with molecular mass of 85 kDa Escherichia coli bacteria as an adhesin and a hemagglutinin. Methods: This study is a true experimental laboratories and a descriptive study to determine roles of pili protein with molecular mass of 85 kDa as an adhesin and a hemagglutinin. Samples used in this study were a stock of E. coli in the Microbiology Laboratory Faculty of Medicine, Universitas Negeri Jember (UNEJ). A hemagglutination test in this study used mouse erythrocytes while an adhesion test used mouse enterocytes. Isolation and purification of E. coli pili protein, isolation of mouse erythrocytes, isolation of mouse enterocytes, the hemagglutination test and the adhesion test were methods in this study. A data analysis with a correlation-regression was used to determine relationships between the adhesion index and titer pili 85 kDa with a limit of significance, 0.05 (p <0.05). Results: The hemagglutination test showed no ability of agglutination with the formation of red aggregate points starting from the first dilution. An analysis with Pearson correlation indicated a relationship between titer pili 85 kDa E. coli and adhesion index (p = 0.009; R = -0.921).Conclusion: Pili protein 85 kDa E. coli plays a role as an adhesin protein and do not have a role as a hemagglutinin protein.
Hubungan antara Higiene Perorangan dan Kejadian Koinfeksi Cacingan pada Penderita Tuberkulosis Lailatis Shofia; Bagus Hermansyah; Enny Suswati; Dini Agustina; Diana Chusna Mufida; Muhammad Ali Shodikin
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Vol 4, No 1, 2021
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v4i1.98

Abstract

Tuberkulosis (TB) dan cacingan merupakan penyakit infeksi dengan jumlah penderita yang banyak di Indonesia. Tingginya prevalensi cacingan di Indonesia memungkinkan terjadinya koinfeksi STH pada pasien TB paru yang menyebabkan imunitas anti M. tuberculosis menurun sehingga respon terhadap pengobatan tuberkulosis menjadi tidak maksimal. Cacingan salah satunya dipengaruhi oleh higiene perorangan. Tujuan penelitian ini adalah untuk menganalisis hubungan antara higiene perorangan dan kejadian koinfeksi cacingan pada penderita TB di Kecamatan Tempurejo Kabupaten Jember. Jenis penelitian ini adalah penelitian observasional dengan desain analitik cross sectional. Populasi yang digunakan adalah pasien TB di Kecamatan Tempurejo dalam periode waktu September – Oktober 2019. Data yang digunakan dalam penelitian ini adalah data primer berupa hasil wawancara dan hasil pemeriksaan feses. Pemeriksaan feses dilakukan menggunakan metode sedimentasi dan floatasi. Analisis data dilakukan menggunakan uji Fisher. Hasil penelitian ini menunjukkan bahwa kejadian koinfeksi cacingan pada pasien TB di Kecamatan Tempurejo Kabupaten Jember sebesar 9,67% dan disebabkan oleh dua spesies STH yaitu A. lumbricoides (66,7%) dan Hookworm (33,3%). Higiene perorangan responden terdiri atas higiene baik (64,5%) dan higiene buruk (35,5%), dimana 66,7% kejadian koinfeksi cacingan terjadi pada responden dengan higiene perorangan buruk. Hasil uji Fisher menunjukkan bahwa tidak ada hubungan yang signifikan antara higiene perorangan dengan kejadian koinfeksi cacingan pada pasien TB di Kecamatan Tempurejo Kabupaten Jember (p-value sebesar 0,281).
Pengaruh Pemberian Imunisasi Intranasal Epitope Protein RrgB 255-270 Streptococcus pneumoniae Terhadap Kadar IL-4 Danang Tejamukti Widiatmaja; Diana Chusna Mufida; Zahrah Febianti
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Vol 4, No 1, 2021
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v4i1.100

Abstract

Streptococcus pneumoniae atau pneumokokus merupakan penyebab penyakit community acquired pneumoniae(CAP). Penularan pneumokokus dapat dicegah oleh vaksin, seperti PPV dan PCV. Vaksin tersebut memiliki beberapa kelemahan seperti terbatas pada strain tertentu dan pemberian yang masih bersifat invasif. Pada penelitian ini dilakukan pengembangan vaksin dari epitope pneumokokus yang diberikan secara intranasal. Salah satu epitope yang dapat digunakan sebagai kandidat vaksin adalah epitope dari protein RrgB penyusun pili, seperti epitope protein RrgB 255-270 dari bakteri Streptococcus pneumoniae yang memiliki komponen antigenik tinggi. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian imunisasi intranasal epitope protein RrgB 255-270 Streptococcus pneumoniae terhadap kadar IL-4. Kadar IL-4 diukur dengan metode ELISA dari bilasan hidung tikus wistar yang diimunisasi dengan epitope protein RrgB 255-270 S. pneumoniae secara intranasal. Bilasan hidung yang didapat akan diproses menggunakan metode ELISA untuk menghitung kadar IL-4. Nilai rata-rata kadar IL-4 pada K1 adalah 28,852± 18 ng/L, rata-rata kadar IL-4 pada K2 adalah 20,630 ± 9 ng/L dan rata-rata pada K3 adalah 18,519 ± 6 ng/L. Hasil uji ANOVA Welch menunjukkan nilai p sebesar 0,299. Dapat disimpulkan bahwa imunisasi intranasal epitope protein RrgB 255-270 S. pneumoniae memberikan perbedaan yang tidak signifikan (dengan p > 0,05).
Epidemiology of Pulmonary Tuberculosis in Diabetes Mellitus Patients Syahputri, Siti Ananda Hardita; Mufida, Diana Chusna; Bumi, Candra
Jurnal Kesehatan Vol 14 No 2 (2023): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v14i2.3963

Abstract

Pulmonary Tuberculosis (pulmonary TB) and Type 2 Diabetes Mellitus (DMT2) are the leading causes of death worldwide. The prevalence of T2DM has increased worldwide as an aging result, urbanization, changes in diet, and reduced physical activity patterns, which have increased obesity. Pulmonary TB and T2DM often occur together and complicate each other during treatment. Pulmonary TB infection-DMT2 appears based on a low immunity state. When the body has low immunity, the reactivation of Mycobacterium tuberculosis bacteria can occur more easily. However, several factors can influence it. This study uses a case-control design. The researcher made observations on the subjects by comparing the case group and the control group. This study had 74 respondents in the case group and 74 respondents in the control group using quota sampling. This study has the results that the factors that influence the incidence of pulmonary TB-DMT2 are smoking behavior 0.000α, age 0.877>α, and alcohol consumption 0.998>α. (α=0.05). The factor that most influences the risk of pulmonary TB-DMT2 is smoking behavior with a wald value of 13.391. This research provides information regarding the problem of pulmonary TB-DMT2 in Jember. To increase public awareness of the risk for Pulmonary TB-DMT2.
The Relationship Between Personal Hygiene and The Prevalence of Tinea Unguium Infection Among Farmers in Jember District Mufida, Diana Chusna; Himma, Alyatul; Agustina, Dini; Armiyanti, Yunita; Sofiana, Kristianningrum Dian
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 18 No 1 (2024): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v18i1.7514

Abstract

Tinea unguium is an infection of the nail caused by dermatophyte fungi. Nails infected with the fungus will be discolored, thick, and brittle. Personal hygiene is one of the risk factors that can affect tinea unguium. This study aimed to determine the relationship of personal hygiene to the prevalence of tinea unguium infection in farmers in the Jember Regency. The benefits of research can be used as a source of information about the importance of maintaining personal hygiene to avoid fungal infections that cause tinea unguium. This study used an analytic observational method with a cross-sectional approach. This research was conducted from August to December 2023 in 10 districts of Jember Regency. The population in this study were all farmers in Jember Regency. The sample in this study was calculated by the Slovin formula and obtained from as many as 100 farmers who met the inclusion and exclusion criteria. Sampling using mixed methods sampling. The statistical analysis used is Cramer's V test α = 0.05. The results of this study show a significant relationship between personal hygiene and the prevalence of tinea unguium in farmers in Jember Regency with a p-value of 0.004 (0,004<0,05).
Effect of Immunization of The Pili Protein 65.5 kDa Klebsiella pneumoniae on IFN-γ Levels of Spleen BALB/c Mice Sa'adah, Ajeng Samrotu; Mufida, Diana Chusna; Dini Agustina; Pulong Wijang Pralampita
Indonesian Journal of Tropical and Infectious Disease Vol. 12 No. 2 (2024)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v12i2.54026

Abstract

Klebsiella pneumoniae is a Gram-negative bacterium that poses a threat to the global community. Currently, no vaccine for K. pneumoniae is licensed by the Food and Drug Administration (FDA). The delay in the manufacture of the K. pneumoniae vaccine was because many vaccine candidates failed at the clinical trial stage due to adverse cross-reactions. Pili can be used as a choice as a vaccine candidate. Pili K. pneumoniae is an immunogenic substance that triggers an immune response, one of which is the cytokine IFN-γ. Splenic splenocytes are the main source of IFN-γ-producing cells. The aim of this study is to determine the effect of immunization pili protein 65.5 kDa K. pneumoniae on IFN-γ levels from spleen BALB/c mice. There were 3 groups, K1 as control given PBS, K2 given pili protein 65.5 kDa + adjuvant, and K3 given adjuvant. IFN-γ was then measured by the ELISA method and analyzed by the ANOVA test. The results of measuring IFN-γ levels using One-way ANOVA showed that the total for all groups was 243.50 ± 43.7 with p < 0.05, the Post Hoc LSD test was continued. The Post Hoc test showed significant differences between K1 control and K2 groups, and between K1 and K3 groups, but not between K2 and K3 groups. It can be concluded that immunization with 65.5 kDa of pili protein does not affect the increase in IFN-γ levels in the spleen of BALB/c mice. 
Increased levels of IL-4 in the Spleen of BALB/c Mice after 65.5 kDa Pili Protein Klebsiella pneumoniae Immunization Putra Uta, Diego Rafi; Mufida, Diana Chusna; Sutejo, Ika Rahmawati; Agustina, Dini; Suswati, Enny; Shodikin, Muhammad Ali
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.575

Abstract

Pneumonia is an infection of the lung parenchyma caused by Klebsiella pneumoniae, resulting in a high mortality rate of millions each year. To reduce these deaths, one potential solution is to create a vaccine that utilizes virulence factors of this microorganism, such as pili. During the early phase of infection, these antigens have a crucial role and can stimulate the production of memory b cells. These cells are activated by IL-4 (interleukin-4) in lymphoid organs, such as the spleen. Pathogen exposure such as virulence factors can stimulate the secretion of IL-4 in the spleen organ. This study aims to investigate the role of Klebsiella pneumoniae pili in this process. The research conducted from May to December 2023 at the Microbiology Laboratory, Faculty of Medicine, University of Jember was purely experimental, using Balb/c mice with IL-4 levels in the spleen organ as variables. The study used 15 mice, divided into control (K1), adjuvant (K2), and antigen (K3) groups. The research data were analyzed using non-parametric tests, specifically the Kruskal-Wallis and Post Hoc tests. The Kruskal-Wallis test revealed significant differences (p=0.003). In the Post Hoc test, a significant difference was found between the control and antigen groups (p=0.002). The results conclusively demonstrate that induction of Klebsiella pneumoniae pili protein 65.5 kDa significantly increases IL-4 levels in the spleen. Future studies should consider adding serum specimens to provide additional information.
The 32 kDa Outer Membrane Proteins of Klebsiella pneumoniae Acts as A Bacterial Adhesin Agustina, Dini; Shodikin, M. Ali; Nugraha, Bima Setia Sandya; Mufida, Diana Chusna; Suswati, Enny; Hermansyah, Bagus
Jurnal ILMU DASAR Vol 23 No 2 (2022)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v23i2.25435

Abstract

Klebsiella pneumoniae is a bacterium that often causes infection in the human body. At present K. pneumoniae can resist some of the antibiotics it has associated with modification of one of the virulence factors possessed by K. pneumoniae. One virulence factor of K. pneumoniae as pathogen bacteria is Outer Membrane Protein (OMP). The study of adhesin factors in K. pneumoniae involving hemagglutinin and adhesin proteins that have been found in the OMP 20 kDa and 40 kDa, but there is still no research that discusses the role of 32 kDa OMP as a hemagglutinin protein and adhesin. The purpose of this study is to determine the role of 32 kDa outer membrane of K. pneumoniae as hemagglutinin and adhesin proteins. After isolation of Outer Member Protein (OMP) from the K. pneumoniae, which then carried out a hemagglutination test using mice erythrocyte cells and adhesion test using mice enterocyte cells. The results of the hemagglutination test using mice erythrocyte cells obtained the highest hemagglutination titer for the molecular weight of 32 kDa in titers 1/4. The adhesion index with dilution titer has a significant relationship, with a conversion coefficient of 0,813 which means the dilution titer with the OMP adhesion index has a strong relationship with the direction of a positive relationship. The regression test results obtained an R-value of 0,813 which shows a strong relationship, while the R2 value is 66.1%. Conclusion in this study is 32 kDa outer membrane proteins of K. pneumoniae acts as a bacterial adhesin.
The Association between Environmental Sanitation and Soil Transmitted Helminths (STH) Coinfection of Tuberculosis Patients in Panti District, Jember Regency Alfianti, Nita; Armiyanti, Yunita; Hermansyah, Bagus; Suswati, Enny; Agustina, Dini; Mufida, Diana Chusna
Journal of Ners and Midwifery Vol 7 No 3 (2020)
Publisher : STIKes Patria Husada Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26699/jnk.v7i3.ART.p354-361

Abstract

Tuberculosis (TB) is a contagious infectious disease caused by the bacteria Mycobacterium tuberculosis. More than 80% of TB cases attack the pulmonary organs and the rest are extra-pulmonary TB. Indonesia is one of the countries with the high number of tuberculosis cases besides India, China, the Philippines, and Pakistan. Aside from being a country with a high TB incidence, Indonesia is also an endemic country for helminth infections, especially Soil-Transmitted Helminths (STH). The high prevalence of STH infections in Indonesia allows STH coinfection in TB patients to cause decreasing immunity, thus affecting the outcome of TB infection. STH infection is very closely related to environmental sanitation. The purpose of this study was to identify and determine the association of environmental sanitation risk factors to the coinfection of Soil-Transmitted Helminths (STH) in tuberculosis patients in Panti District, Jember Regency. This type of research was observational research with cross-sectional analytic design. Of the 49 TB patients who were undergoing treatment at the Panti Health Center, 32 people were willing to be respondents, but only 25 people collected stool samples. Fisher's test results showed that the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency was 12% and was caused by two STH species, namely A. lumbricoides and Hookworm. Environmental sanitation in TB patients was mostly good, e.g 54.5% of respondents had good environmental sanitation, but there was no significant association between environmental sanitation and the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency (p> 0.05).
Co-Authors Adellia Fira Fa'idha Adilah Julinar Irianti Alfianti, Nita Angga Mardro Raharjo Angga Mardro Raharjo, Angga Mardro Azham Purwandhono Bagus Hermansyah Candra Bumi Dafista Diyantika Dafista Diyantika Danang Tejamukti Widiatmaja Dini Agustina Dion Krismashogi Dharmawan Dwita Aryadina Rachmawati Ellen Ocktavironita Elvia Rahmi Marga Putri Enny Suswanti ENNY SUSWATI Eprila Darma Sari Fitri, Nadya Eka Hanifa Rizki A.S. Heni Fatmawati Himma, Alyatul Iftinan, Karenzha Ika Rahmawati Sutejo Kristianningrum Dian Sofiana Lailatis Shofia M. Ali Shodikin Mei Syafriadi Misnawi Misnawi Misnawi Misnawi Nita Alfianti Nugraha, Bima Setia Sandya Nurul Indah Saffanah Pralampita, Pulong Wijang Putra Uta, Diego Rafi Putu Ayu Laksmi Lestari Qintar Layallia Faza Regina Finka S.Pd. M Kes I Ketut Sudiana . Sa'adah, Ajeng Samrotu Salsabila, Yuna Annisa Saputra, Antonius Dwi Shodikin, M. Ali Shofia, Lailatis Siti Marissa Aisyah Syahputri, Siti Ananda Hardita Wahyuningsih, dr. Rita Widhiasari Normaningtyas Widiatmaja, Danang Tejamukti Wiwien Sugih Utami Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Zahrah Febianti