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All Journal International Journal of Public Health Science (IJPHS) Jurnal Kesehatan Lingkungan indonesia Jurnal Ilmu Dasar Jurnal Natur Indonesia e-Journal Pustaka Kesehatan Jurnal Kesehatan Masyarakat Indonesian Journal of Tropical and Infectious Disease Journal of Agromedicine and Medical Sciences Elkawnie The Indonesian Journal of Occupational Safety and Health Majalah Kedokteran Sriwijaya Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) Medical Laboratory Technology Journal Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan SRIWIJAYA JOURNAL OF MEDICINE Jurnal Respirologi Indonesia Jurnal Kesehatan Journal of Health Sciences Poltekita : Jurnal Ilmu Kesehatan Berkala Penelitian Hayati JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Berita Kedokteran Masyarakat Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery)
Diana Chusna Mufida
Departement Mikrobiologi, Fakultas Kedokteran, Universitas Jember, Jl. Kalimantan No.37, Kampus Tegal Boto, Kabupaten Jember, Jawa Timur, 68121
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health

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Personal Protective Footwear and The Risk of Tinea Unguium among Lojejer Villager Farmers Iftinan, Karenzha; Raharjo, Angga Mardro; Agustina, Dini; Mufida, Diana Chusna
The Indonesian Journal of Occupational Safety and Health Vol. 14 No. 2 (2025): The Indonesian Journal of Occupational Safety and Health
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijosh.v14i2.2025.222-229

Abstract

Introduction: Tinea unguium is a nail infection caused by dermatophytes, characterized by nail discoloration, thickening, and brittleness. Farmers, often exposed to prolonged wet conditions, are at higher risk of this infection. The use of appropriate footwear is recommended to mitigate this risk. However, inadequate personal protective equipment (PPE) usage makes farmers more susceptible to Tinea unguium. Research on Tinea unguium among farmers in Lojejer Village is limited, with differing result on the link between wearing footwear as PPE and the occurance of Tinea unguium. This study aimed to investigate the relationship between the use of footwear as PPE and the incidence of Tinea unguium among farmers in Lojejer Village. Methods: This study uses a cross-sectional observational design. It involved 98 respondents determined using Slovin’s formula. Data were collected through interviews and nail samples, which were subjected to fungal culture on sabouraud dextrose agar (SDA) medium and microscopic examination with lactophenol cotton blue staining. Fisher’s Exact tests were used for analysis. Results: Results revealed that seven farmers (7.14%) were affected by Tinea unguium, predominantly males (85.8%) aged 45–64 years (57.1%). Trichophyton rubrum was identified as the primary dermatophyte, with Aspergillus sp. as a contaminant. Statistical analysis showed no significant relationship between footwear usage, personal hygiene, or footwear hygiene and Tinea unguium (p-values > 0.05). Conclusion: The study found no significant association between footwear use, hpersonal hygiene, and footwear hygiene with Tinea unguium incidence among farmers in Lojejer Village
Bacterial contamination of tomatoes sellers in eight traditional markets of Jember Regency, East Java, Indonesia Laili, Nikmatul; Suswati, Enny; Abrori, Cholis; Mufida, Diana Chusna; Hermansyah, Bagus; Putri, Elvia Rahmi Marga
BKM Public Health and Community Medicine Vol 40 No 04 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bkm.v40i04.9307

Abstract

Purpose: Foodborne diseases are a significant threat to public health and is often associated with pathogenic microorganisms entering the body due to consuming contaminated food. One of the factors causing foodborne diseases was the contamination of pathogenic bacteria in vegetables consumed raw, one of which is tomatoes. This study was conducted to determine the correlation between traders' personal hygiene behavior and bacterial contamination of tomatoes in the traditional Jember Regency market. Methods: This study used analytical observational research with a cross-sectional research design, conducted in the Laboratory of Microbiology, Faculty of Medicine, University of Jember. The large sample size of this study was 40 traders. Personal hygiene behavior of traders was obtained from direct observation with observation sheets in eight selected traditional markets in Jember Regency. Results: Tomato samples were contaminated with bacteria with the highest bacterial colony >2.5 x 108 CFU/g and the lowest 4.6 x 105 CFU/g. The types of bacteria found in this study were Vibrio parahaemolyticus (15%), Shigella sp. (62.5%), Salmonella sp. (72.5%), Vibrio cholerae (82.5%), Escherichia coli (90%), and Staphylococcus aureus (92.5%). The bivariate test results showed no significant correlation between personal hygiene, sanitation, supporting facilities, and traders' characteristics with bacterial contamination of tomatoes sold in eight Jember traditional markets. Conclusion: This study concludes that there is no significant correlation between traders' personal hygiene behavior and bacterial contamination in tomatoes sold in eight traditional markets of Jember Regency. Consumers should wash tomatoes well or process them first before consumption.
The Relationship between Milking Hygiene and Salmonella sp. Contamination on Cow’s Milk in Jember Regency Mufida, Diana Chusna; Shabri, Hammam Arif; Hermansyah, Bagus; Shodikin, M Ali; Suswati, Enny
BKM Public Health and Community Medicine The 12th UGM Public Health Symposium
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Purpose: Milk is a liquid produced by the mammary glands of female mammals. The nutritional content of milk almost covers the entire nutritional needs required by living things. The appearance of Salmonella sp. in cow's milk is due to milking methods that do not meet the standards, especially regarding milking hygiene management. This study examines the relationship between milking hygiene and Salmonella sp. contamination in cow's milk in Jember Regency. Method: This study used observational analytic research with a cross-sectional research design. Primary data were obtained through direct observation of milkers using a checklist related to milking. observe Salmonella sp. contamination using culture techniques on SCA (Salmonella Chromogenic Agar) as selective media agar for Salmonella sp. and Gram staining. Results: The results from this study show that the hygiene process pre-milking (78.9%), during-milking (71.1%), and post-milking (89.5%) have been implemented. The statistical test results showed a relationship between the pre-milking hygiene process with Salmonella sp. contamination (p-value < 0.05), but insignificant results were seen in the during and post-milking hygiene processes (p-value> 0.05). Conclusion: The conclusion from this study show that the three results only the hygiene processes pre-milking have results associated with Salmonella sp. contamination in cows' milk. Therefore, milkers are expected to maintain and improve dairy hygiene measures, especially before milking to prevent contamination of cow's milk.
Predisposing Factors in Preventing The Transmission of Drug-Resistant TBC: A Qualitative Study in Jember Atmawati, Siti Dwiana; Fajrin, Fifteen Aprila; Mufida, Diana Chusna
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 3 (2023): November
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i3.3173

Abstract

Drug-Resistant Tuberculosis (DR TB) is a form of tuberculosis caused by Mtb bacteria resistant to anti-tuberculosis drugs (OAT). The prevalence of DR TB in Jember has significantly increased over the years, and many still refuse treatment. These individuals actively spread the bacteria to people at home or in the workplace. This research aims to identify predisposing factors in preventing the transmission of DR TB based on knowledge, attitudes, and beliefs. It is a qualitative case study involving primary subjects, DR TB patients, additional information from patient support organizations, and health department officials in Kab. Jember. The criteria for research subjects are residing in Kab. Jember, undergoing treatment, and willing to participate. The subject selection technique uses purposive sampling, resulting in 10 individuals. Data collection techniques involve in-depth interviews and observations. Data presentation follows the guidelines of Milles and Huberman, employing Thematic Content Analysis (TCA) for analysis. Data validity checks use credibility and dependability tests with source and technique triangulation. The research results indicate varying knowledge among subjects regarding DR TB prevention, with most obtaining information from healthcare professionals and patient support. In terms of attitude perspectives, all subjects show a positive response, expressing willingness to prevent DR TB transmission. Belief perspectives reveal diverse actions in DR TB transmission prevention. Most trust advice from healthcare professionals and patient support, while a minority still believe in herbal treatments and the culture of "tirah" to support TB treatment. In conclusion, knowledge, attitude, and belief perspectives are positive, but there is room for improvement in the behavior of implementing DR TB transmission prevention measures.
B-cell epitope prediction of MPB83 protein as a candidate for serodiagnostic antigen of bovine tuberculosis in human: In silico study Ahmad, Shabrina Izzata Afiyati; Aziz , Ayu Munawaroh; Armiyanti, Yunita; Mufida, Diana Chusna
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 2, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss2.art4

Abstract

Background: Bovine tuberculosis (bTB) can be transmitted to humans by inhalation or consumption of incomplete pasteurized milk and dairy products derived from infected cows. Most cases of Mycobacterium tuberculosis (M. bovis) infection are resistant to tuberculosis (TB) drugs. The risk of death during treatment for bTB has been reported to be 2.55 times higher than for TB. However, the quality of diagnostic methods for bTB remains relatively low.Objective: We aim to evaluate the potential of the B-cell epitope of the MPB83 protein as a candidate bTB serodiagnostic antigen using an in silico approach. Methods: This study was a computer-based descriptive study using secondary data from the National Center for Biotechnology Information (NCBI) protein database. The MPB83 protein sequence was obtained from M. tuberculosis variant bovis AF2122/97 from the United Kingdom. We described the characteristics of the linear epitope of the M. bovis B-cell protein MPB83 by measuring antigenicity, molecular weight, instability index, and Grand Average of Hydropathy (GRAVY) score. The tools used in this study were IBIVU PRALINE, VaxiJen v2.0, IEDB, ExPASy ProtParam, Cluspro, and the PyMOL application.Results: We found an epitope that could be used for bTB serodiagnostic antigen with low conservation, the 106KLNPDVNLVDTLN118 epitope. It has the molecular weight, instability index, and GRAVY score of 1638.76 Da, -28.44, and -0.300, respectively. Epitopes with the best criteria were simulated by docking to human major histocompatibility complex (MHC) class II. Docking results showed that the lowest binding energy was -644.8 kcal/mol. Further analysis using the PyMOL application obtained 14 hydrogen bonds with bond distances ranging from 1.7 Å to 2.2 Å, all of which showed strong hydrogen bonds.Conclusion: The B-cell epitope of MPB83 protein sequence 106KLNPDVNLVDTLN118 has a potential serodiagnostic antigen candidate for human bTB.
The Association between Environmental Sanitation and Soil Transmitted Helminths (STH) Coinfection of Tuberculosis Patients in Panti District, Jember Regency Nita Alfianti; Yunita Armiyanti; Bagus Hermansyah; Enny Suswati; Dini Agustina; Diana Chusna Mufida
Journal of Ners and Midwifery Vol 7 No 3 (2020)
Publisher : STIKes Patria Husada Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26699/jnk.v7i3.ART.p354-361

Abstract

Tuberculosis (TB) is a contagious infectious disease caused by the bacteria Mycobacterium tuberculosis. More than 80% of TB cases attack the pulmonary organs and the rest are extra-pulmonary TB. Indonesia is one of the countries with the high number of tuberculosis cases besides India, China, the Philippines, and Pakistan. Aside from being a country with a high TB incidence, Indonesia is also an endemic country for helminth infections, especially Soil-Transmitted Helminths (STH). The high prevalence of STH infections in Indonesia allows STH coinfection in TB patients to cause decreasing immunity, thus affecting the outcome of TB infection. STH infection is very closely related to environmental sanitation. The purpose of this study was to identify and determine the association of environmental sanitation risk factors to the coinfection of Soil-Transmitted Helminths (STH) in tuberculosis patients in Panti District, Jember Regency. This type of research was observational research with cross-sectional analytic design. Of the 49 TB patients who were undergoing treatment at the Panti Health Center, 32 people were willing to be respondents, but only 25 people collected stool samples. Fisher's test results showed that the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency was 12% and was caused by two STH species, namely A. lumbricoides and Hookworm. Environmental sanitation in TB patients was mostly good, e.g 54.5% of respondents had good environmental sanitation, but there was no significant association between environmental sanitation and the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency (p> 0.05).
Co-Authors Adellia Fira Fa'idha Adilah Julinar Irianti Ahmad, Shabrina Izzata Afiyati Angga Mardro Raharjo Angga Mardro Raharjo, Angga Mardro Atmawati, Siti Dwiana Azham Purwandhono Aziz , Ayu Munawaroh Bagus Hermansyah Candra Bumi Cholis Abrori, Cholis Dafista Diyantika Dafista Diyantika Danang Tejamukti Widiatmaja Dini Agustina Dion Krismashogi Dharmawan Dwita Aryadina Rachmawati Ellen Ocktavironita Elvia Rahmi Marga Putri Enny Suswanti ENNY SUSWATI Eprila Darma Sari Fifteen Aprila Fajrin Fitri, Nadya Eka Hanifa Rizki A.S. Heni Fatmawati Himma, Alyatul Iftinan, Karenzha Ika Rahmawati Sutejo Kristianningrum Dian Sofiana Lailatis Shofia Laili, Nikmatul M. Ali Shodikin Mei Syafriadi Misnawi Misnawi Misnawi Misnawi Nita Alfianti Nugraha, Bima Setia Sandya Nurul Indah Saffanah Pralampita, Pulong Wijang Putra Uta, Diego Rafi Putri, Elvia Rahmi Marga Putu Ayu Laksmi Lestari Qintar Layallia Faza Regina Finka S.Pd. M Kes I Ketut Sudiana . Sa'adah, Ajeng Samrotu Salsabila, Yuna Annisa Saputra, Antonius Dwi Shabri, Hammam Arif Shodikin, M Ali Shodikin, M. Ali Shofia, Lailatis Siti Marissa Aisyah Syahputri, Siti Ananda Hardita Wahyuningsih, dr. Rita Widhiasari Normaningtyas Widiatmaja, Danang Tejamukti Wiwien Sugih Utami Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Zahrah Febianti