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Bacterial contamination of tomatoes sellers in eight traditional markets of Jember Regency, East Java, Indonesia Laili, Nikmatul; Suswati, Enny; Abrori, Cholis; Mufida, Diana Chusna; Hermansyah, Bagus; Putri, Elvia Rahmi Marga
BKM Public Health and Community Medicine Vol 40 No 04 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bkm.v40i04.9307

Abstract

Purpose: Foodborne diseases are a significant threat to public health and is often associated with pathogenic microorganisms entering the body due to consuming contaminated food. One of the factors causing foodborne diseases was the contamination of pathogenic bacteria in vegetables consumed raw, one of which is tomatoes. This study was conducted to determine the correlation between traders' personal hygiene behavior and bacterial contamination of tomatoes in the traditional Jember Regency market. Methods: This study used analytical observational research with a cross-sectional research design, conducted in the Laboratory of Microbiology, Faculty of Medicine, University of Jember. The large sample size of this study was 40 traders. Personal hygiene behavior of traders was obtained from direct observation with observation sheets in eight selected traditional markets in Jember Regency. Results: Tomato samples were contaminated with bacteria with the highest bacterial colony >2.5 x 108 CFU/g and the lowest 4.6 x 105 CFU/g. The types of bacteria found in this study were Vibrio parahaemolyticus (15%), Shigella sp. (62.5%), Salmonella sp. (72.5%), Vibrio cholerae (82.5%), Escherichia coli (90%), and Staphylococcus aureus (92.5%). The bivariate test results showed no significant correlation between personal hygiene, sanitation, supporting facilities, and traders' characteristics with bacterial contamination of tomatoes sold in eight Jember traditional markets. Conclusion: This study concludes that there is no significant correlation between traders' personal hygiene behavior and bacterial contamination in tomatoes sold in eight traditional markets of Jember Regency. Consumers should wash tomatoes well or process them first before consumption.
The Relationship between Milking Hygiene and Salmonella sp. Contamination on Cow’s Milk in Jember Regency Mufida, Diana Chusna; Shabri, Hammam Arif; Hermansyah, Bagus; Shodikin, M Ali; Suswati, Enny
BKM Public Health and Community Medicine The 12th UGM Public Health Symposium
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Purpose: Milk is a liquid produced by the mammary glands of female mammals. The nutritional content of milk almost covers the entire nutritional needs required by living things. The appearance of Salmonella sp. in cow's milk is due to milking methods that do not meet the standards, especially regarding milking hygiene management. This study examines the relationship between milking hygiene and Salmonella sp. contamination in cow's milk in Jember Regency. Method: This study used observational analytic research with a cross-sectional research design. Primary data were obtained through direct observation of milkers using a checklist related to milking. observe Salmonella sp. contamination using culture techniques on SCA (Salmonella Chromogenic Agar) as selective media agar for Salmonella sp. and Gram staining. Results: The results from this study show that the hygiene process pre-milking (78.9%), during-milking (71.1%), and post-milking (89.5%) have been implemented. The statistical test results showed a relationship between the pre-milking hygiene process with Salmonella sp. contamination (p-value < 0.05), but insignificant results were seen in the during and post-milking hygiene processes (p-value> 0.05). Conclusion: The conclusion from this study show that the three results only the hygiene processes pre-milking have results associated with Salmonella sp. contamination in cows' milk. Therefore, milkers are expected to maintain and improve dairy hygiene measures, especially before milking to prevent contamination of cow's milk.
Predisposing Factors in Preventing The Transmission of Drug-Resistant TBC: A Qualitative Study in Jember Atmawati, Siti Dwiana; Fajrin, Fifteen Aprila; Mufida, Diana Chusna
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 3 (2023): November
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i3.3173

Abstract

Drug-Resistant Tuberculosis (DR TB) is a form of tuberculosis caused by Mtb bacteria resistant to anti-tuberculosis drugs (OAT). The prevalence of DR TB in Jember has significantly increased over the years, and many still refuse treatment. These individuals actively spread the bacteria to people at home or in the workplace. This research aims to identify predisposing factors in preventing the transmission of DR TB based on knowledge, attitudes, and beliefs. It is a qualitative case study involving primary subjects, DR TB patients, additional information from patient support organizations, and health department officials in Kab. Jember. The criteria for research subjects are residing in Kab. Jember, undergoing treatment, and willing to participate. The subject selection technique uses purposive sampling, resulting in 10 individuals. Data collection techniques involve in-depth interviews and observations. Data presentation follows the guidelines of Milles and Huberman, employing Thematic Content Analysis (TCA) for analysis. Data validity checks use credibility and dependability tests with source and technique triangulation. The research results indicate varying knowledge among subjects regarding DR TB prevention, with most obtaining information from healthcare professionals and patient support. In terms of attitude perspectives, all subjects show a positive response, expressing willingness to prevent DR TB transmission. Belief perspectives reveal diverse actions in DR TB transmission prevention. Most trust advice from healthcare professionals and patient support, while a minority still believe in herbal treatments and the culture of "tirah" to support TB treatment. In conclusion, knowledge, attitude, and belief perspectives are positive, but there is room for improvement in the behavior of implementing DR TB transmission prevention measures.
B-cell epitope prediction of MPB83 protein as a candidate for serodiagnostic antigen of bovine tuberculosis in human: In silico study Ahmad, Shabrina Izzata Afiyati; Aziz , Ayu Munawaroh; Armiyanti, Yunita; Mufida, Diana Chusna
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 2, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss2.art4

Abstract

Background: Bovine tuberculosis (bTB) can be transmitted to humans by inhalation or consumption of incomplete pasteurized milk and dairy products derived from infected cows. Most cases of Mycobacterium tuberculosis (M. bovis) infection are resistant to tuberculosis (TB) drugs. The risk of death during treatment for bTB has been reported to be 2.55 times higher than for TB. However, the quality of diagnostic methods for bTB remains relatively low.Objective: We aim to evaluate the potential of the B-cell epitope of the MPB83 protein as a candidate bTB serodiagnostic antigen using an in silico approach. Methods: This study was a computer-based descriptive study using secondary data from the National Center for Biotechnology Information (NCBI) protein database. The MPB83 protein sequence was obtained from M. tuberculosis variant bovis AF2122/97 from the United Kingdom. We described the characteristics of the linear epitope of the M. bovis B-cell protein MPB83 by measuring antigenicity, molecular weight, instability index, and Grand Average of Hydropathy (GRAVY) score. The tools used in this study were IBIVU PRALINE, VaxiJen v2.0, IEDB, ExPASy ProtParam, Cluspro, and the PyMOL application.Results: We found an epitope that could be used for bTB serodiagnostic antigen with low conservation, the 106KLNPDVNLVDTLN118 epitope. It has the molecular weight, instability index, and GRAVY score of 1638.76 Da, -28.44, and -0.300, respectively. Epitopes with the best criteria were simulated by docking to human major histocompatibility complex (MHC) class II. Docking results showed that the lowest binding energy was -644.8 kcal/mol. Further analysis using the PyMOL application obtained 14 hydrogen bonds with bond distances ranging from 1.7 Å to 2.2 Å, all of which showed strong hydrogen bonds.Conclusion: The B-cell epitope of MPB83 protein sequence 106KLNPDVNLVDTLN118 has a potential serodiagnostic antigen candidate for human bTB.
The Association between Environmental Sanitation and Soil Transmitted Helminths (STH) Coinfection of Tuberculosis Patients in Panti District, Jember Regency Nita Alfianti; Yunita Armiyanti; Bagus Hermansyah; Enny Suswati; Dini Agustina; Diana Chusna Mufida
Journal of Ners and Midwifery Vol 7 No 3 (2020)
Publisher : STIKes Patria Husada Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26699/jnk.v7i3.ART.p354-361

Abstract

Tuberculosis (TB) is a contagious infectious disease caused by the bacteria Mycobacterium tuberculosis. More than 80% of TB cases attack the pulmonary organs and the rest are extra-pulmonary TB. Indonesia is one of the countries with the high number of tuberculosis cases besides India, China, the Philippines, and Pakistan. Aside from being a country with a high TB incidence, Indonesia is also an endemic country for helminth infections, especially Soil-Transmitted Helminths (STH). The high prevalence of STH infections in Indonesia allows STH coinfection in TB patients to cause decreasing immunity, thus affecting the outcome of TB infection. STH infection is very closely related to environmental sanitation. The purpose of this study was to identify and determine the association of environmental sanitation risk factors to the coinfection of Soil-Transmitted Helminths (STH) in tuberculosis patients in Panti District, Jember Regency. This type of research was observational research with cross-sectional analytic design. Of the 49 TB patients who were undergoing treatment at the Panti Health Center, 32 people were willing to be respondents, but only 25 people collected stool samples. Fisher's test results showed that the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency was 12% and was caused by two STH species, namely A. lumbricoides and Hookworm. Environmental sanitation in TB patients was mostly good, e.g 54.5% of respondents had good environmental sanitation, but there was no significant association between environmental sanitation and the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency (p> 0.05).
Immunodetection of Adhesin Pili Protein 38.6 kDa K. pneumoniae Using Western Blot Dini Agustina; Diana Chusna Mufida; Nurul Indah Saffanah
Elkawnie Vol. 7 No. 2 (2021)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i2.10127

Abstract

Abstract: Klebsiella pneumoniae is a pathogen that causes infections in communities and hospitals. These bacteria have many virulence factors that play an important role in the pathogenicity of infection and antibiotic resistance. Pili protein Klebsiella pneumoniae with 38.6 kDa as one of the virulence factors, that have roles as hemagglutinin dan adhesin protein so potentially as a vaccine candidate. This research aimed to immunodetection pili protein 38.6 kDa using Western Blot.  Western Blot was used to detect that protein with specific primary antibodies. These antibodies are obtained from mice serum-induced intraperitoneally with Pili protein 38.6 kDa Klebsiella pneumoniae. Protein bands that appear on the membrane of Western Blot results are proteins with a molecular weight of 85.6 kDa, 65.5 kDa, 46.9 kDa, and 29.4 kDa. This study concludes that Pili protein 38.6 kDa Klebsiella pneumoniae as the target protein does not appear in the Western Blot result.Abstrak: Klebsiella pneumoniae adalah patogen yang menyebabkan infeksi di komunitas maupun rumah sakit. Bakteri tersebut memiliki banyak faktor virulensi yang berperan penting dalam patogenisitas terjadinya infeksi dan resistensi antibiotik. Protein pili Klebsiella pneumoniae dengan 38,6 kDa sebagai salah satu faktor virulensi, memiliki peran sebagai protein hemagglutinin dan adhesin sehingga berpotensi sebagai kandidat vaksin. Tujuan penelitian ini untuk imunodeteksi protein pili 38,6 kDa dengan menggunakan Western Blot. Western Blot digunakan untuk mendeteksi protein itu dengan spesifik antibodi primer. Antibodi ini diperoleh dari serum tikus yang telah diinduksi secara inteperitoneal dengan protein Pili 38,6 kDa Klebsiella pneumoniae. Band protein yang muncul pada membran hasil Western Blot adalah protein dengan berat molekul 85,6 kDa; 65,5 kDa; 46,9 kDa dan 29,4 kDa. Dari penelitian ini dapat disimpulkan bahwa protein Pili 38.6 kDa Klebsiella pneumoniae sebagai protein target tidak muncul.
The Relationship Between Personal Hygiene and The Prevalence of Tinea Unguium Infection Among Farmers in Jember District Diana Chusna Mufida; Alyatul Himma; Dini Agustina; Yunita Armiyanti; Kristianningrum Dian Sofiana
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 18 No 1 (2024): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v18i1.7514

Abstract

Tinea unguium is an infection of the nail caused by dermatophyte fungi. Nails infected with the fungus will be discolored, thick, and brittle. Personal hygiene is one of the risk factors that can affect tinea unguium. This study aimed to determine the relationship of personal hygiene to the prevalence of tinea unguium infection in farmers in the Jember Regency. The benefits of research can be used as a source of information about the importance of maintaining personal hygiene to avoid fungal infections that cause tinea unguium. This study used an analytic observational method with a cross-sectional approach. This research was conducted from August to December 2023 in 10 districts of Jember Regency. The population in this study were all farmers in Jember Regency. The sample in this study was calculated by the Slovin formula and obtained from as many as 100 farmers who met the inclusion and exclusion criteria. Sampling using mixed methods sampling. The statistical analysis used is Cramer's V test α = 0.05. The results of this study show a significant relationship between personal hygiene and the prevalence of tinea unguium in farmers in Jember Regency with a p-value of 0.004 (0,004<0,05).
Caspase-3 modulation by Cinnamomum burmannii extract attenuates pancreatic damage in diabetic rats: An in silico and in vivo study Sulihah, Nurfaizah Titisari; Mufida, Diana Chusna; Akbar, Anandiya Dzakyfikri Dzikrullah; Baidowi, Ivan Iqbal
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 17, No 2, (2026)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol17.Iss2.art2

Abstract

Background: Diabetes mellitus is characterized by hyperglycaemia resulting from impaired insulin secretion or action. This condition may trigger oxidative stress leading to apoptosis, mediated by caspase-3. Pancreatic tissue damage caused by apoptosis contributes to diabetes progression. Cinnamomum burmannii extract is known to contain bioactive compounds with antioxidant and antiapoptotic properties, which may provide protective effects on pancreatic tissue.Objectives: This study aimed to evaluate the potential of C. burmannii extract in modulating caspase-3 in pancreatic diabetic rats through both in silico and in vivo studies.Method: The in silico study was conducted using molecular docking analysis to evaluate the binding affinity and interaction patterns of active compounds from C. burmannii with Caspase-3 as the target protein involved in apoptosis using pyRx, with visualization in Discovery Studio. The in vivo study used streptozotocin-induced diabetic male Wistar rat, which were randomly divided into four groups (normal control group, DM group, DM group treated with C. burmannii extract 100 mg/kg BW, DM group treated with CB extract 200 mg/kg BW). The pancreatic tissue was collected, and the Caspase-3 expression was evaluated using immunohistochemical staining. The obtained data were analysed using SPSS Statistics with one-way ANOVA and Pearson correlation coefficient, with p < 0.05 considered significant.Results: The in silico analysis demonstrated that epicatechin compounds have a strong binding affinity with the active site of caspase-3. Then the in vivo analysis indicated that administration of C. burmannii extract significantly reduced caspase-3 expression in the pancreas of the diabetic rats compared to the control group.Conclusion: C. burmannii extract has potential as an antiapoptotic agent through caspase-3 modulation. These findings provide scientific evidence supporting the promising role of C. burmannii as an adjunctive therapeutic agent in diabetes management.
Meningococcal Meningitis In Children: A Case Report Muhammad Ali Shodikin; Achmad Haykal Baswedan; Dini Agustina; Diana Chusna Mufida; Enny Suswati; Bagus Hermansyah
Journal of Clinical and Translational Medicine Vol. 1 No. 1 (2026): August
Publisher : PT Litera Integra Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.67580/medixis.v1i1.87

Abstract

Meningococcal meningitis is brain membrane (meninges) inflammation caused by Neisseria meningitidis (Meningococcus) bacteria. In the child population, meningococcal meningitis is a rapid-onset disease with high morbidity and mortality rates. In this case, a 7-year-old boy admitted to the Emergency Room of dr. Soebandi Hospital, Jember, presented with seizure, decreased of consciousness, fever, and positive meningeal signs. The results of laboratory and radiological investigations showed bacterial meningitis caused by Neisseria meningitidis bacteria. This patient was treated with antibiotics, symptomatic and supportive medication, which gave a good result. It is important to publish all clinical findings, laboratory examinations, and management of this case so that health workers are aware of the spread possibility of meningococcal meningitis.