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All Journal ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Hemera Zoa Jurnal Sain Veteriner Spirakel Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Current Biomedicine
Elok Budi Retnani
Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis, Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Upaya Meningkatkan Kesadaran Higiene Personal Masyarakat dan Menjamin Kesejahteraan Hewan Ternak dalam Menghadapi Pandemi Covid-19 Elok Budi Retnani; Rudy Rudy; Desi Nupremani Dermawan; Nadira Fadilah; Felicia Rizal Putri; Virgilius Martin Kelake Kedang; Berlin Berliana Anugrah; Hilman Rizki Pangestu; Januarius Jimi
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. Khusus 2 (2021): April 2021
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.864 KB)

Abstract

Thematic community service program which are located in Neglasari and Purwasari villages, Dramaga sub-district, Bogor district has been going well. Several program activities are carried out including the prevention and handling of Covid-19 which aims to prepare the people of Neglasari and Purwasati villages to face the Covid -19 pandemic. The next program is improving the welfare of farmer groups and livestock health, namely a demonstration of how to make probiotics for livestock through a video and poster which is socialized through the WhatsApp communication group. Another program is “ibu cerdas anak sehat” which aims to educate mothers about zoonotic diseases that are often ignored, such as toxoplasma and ringworm and is implemented by distributing leaflets to mothers who attend posyandu. Keywords: COVID-19, Neglasari, Purwasari, Probiotic, Zoonoses
Pendeteksian secara Otomatis Telur Cacing Haemonchus contortus menggunakan Algoritma YOLOv3 Ridi Arif; Elok Budi Retnani; Fadjar Satrija; Rizky Diyu Purnama
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.71945

Abstract

Infeksi Haemonchus contortus atau haemonchosis umumnya terjadi pada ruminansia kecil seperti domba. Haemonchus contortus adalah spesies yang paling patogenik pada ruminansia kecil dan berhabitat di abomasum. Hewan yang terinfeksi Haemonchus contortus  secara berangsur-angsur akan mengalami anemia karena aktivitas cacing yang menghisap darah. Selain menimbulkan anemia, domba akan mengalami penurunan bobot badan akibat penurunan daya cerna. Infeksi tersebut dapat berjalan secara akut maupun kronis dan dalam kondisi tertentu dapat juga mengakibatkan kematian pada hewan. Oleh karena itu dibutuhkan perangkat yang efektif dan efisien untuk mengindentifikasi keberadaan telur cacing Haemonchus contortus sebagai alat deteksi cepat telur cacing. Tujuan dari penelitian ini adalah membuat perangkat cerdas berbasis Algoritma YOLOv3 yang mampu mendeteksi dan mengidentifikasi telur cacing Haemonchus contortus secara cepat.  Penelitian ini menggunakan Algoritma You Only Look Once (YOLO) versi 3 yang merupakan algoritma yang dikembangkan untuk membantu mendeteksi objek secara realtime. Algoritma YOLO dijalankan dalam framework aplikasi anaconda dengan menggunakan pycharm dan aplikasi OPENCV. Identifikasi telur Haemonchus contortus secara otomatis berhasil dilakukan dengan proses tagging pada dataset dan membuat file weight training bagi YOLO. Hasil uji coba menggunakan mikroskop cahaya dan smartphone menunjukan bahwa bahwa Algoritma YOLO mampu mengidentifikasi telur Haemonchus contortus dengan nilai confidence lebih dari 90%. Penggunaan perangkat Dino-Lite yang terhubung pada mikroskop cahaya menunjukan algoritma YOLO tidak dapat berjalan karena adanya enkripsi pada perangkat tersebut. Otomatisasi pendeteksian telur Haemonchus contortus dapat dilakukan dengan memanfaatkan Algoritma YOLOv3 yang dibantu dengan hardware berupa laptop dan smartphone android serta memiliki kemampuan identifikasi dengan tingkat akurasi yang tinggi. 
Aspiculuris tetraptera Infection In Mice: Parasite degree and Differential Leukocyte Zulfikri Mustakdir; Rizal Arifin Akbari; Elok Budi Retnani; Risa Tiuria
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No 2, JULY 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v6i2.20579

Abstract

Aspiculuris tetraptera is one of helminth parasite commonly found in mice. The response of the mice to parasite infection varies depending on various factors. Immune system is one of the factors that affect the occurrence of parasite infection in the host. The purpose of this study were to observe the parasitemia level and the immune respons of mice that infected with Aspiculuris before and after challenge infection. Mice were infected with Aspiculuris twice to see the development of parasite and the immune respon. Feces and blood samples were collected from the mice. Feces samples were examined by floating method to find the parasite egg, whereas blood samples were examined by blood smears to count the differential leukocytes. The result showed that Aspiculuris tetraptera eggs appear in the feces on the 20th day after infected as many 200 eggs/gram and increased on the 26th day as much 300 eggs/gram of feces. The peaks of eosinophils were on the 3rd and 6th days at 2.8% and 3.1% respectively. The level of Neutrophils and monocytes showed fluctuations but the trend decrease from day to day. Lymphocyte levels also showed fluctuations but tended to increase from the first day to the 26th day.
ULTRASOUND IMAGING OF THE SPLEEN, STOMACH, LIVER, KIDNEYS, BLADDER, AND PROSTATE IN PIGLETS (Sus scrofa domestica) Gunanti Gunanti; Carina Khairunnisa; Fadhilah Nur Annisa; Elok Budi Retnani; Akhmad Arif Amin; Dwi Utari Rahmiati
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.276 KB) | DOI: 10.21157/j.ked.hewan.v15i4.21784

Abstract

This study aims to describe ultrasonographic imaging of the spleen, stomach, liver, kidney, bladder, and prostate in piglets (Sus scrofa domestica). Ultrasonography was performed on six male piglets aged 1 month with a body weight of 4-8 kg. Ultrasonography of the spleen, stomach, liver, urinary bladder, and prostate was observed in the dorsal-recumbency position, and the kidney was observed in the lateral-recumbency position. The ultrasound examination used the Chison EBit 60, which is a linear type transducer with a frequency of 9 MHz, transverse and sagittal positions, and a gain of about 55-80. Ultrasonography of the spleen showed that the shape of the spleen head varied in each individual. The results of gastric ultrasonography showed the gastric lining clearly and the similarity of the position, shape, structure, echogenicity, and size of the stomach. The results of liver ultrasonography showed that the echogenicity of the liver tended to be hyperechoic, the kidneys were hyperechoic, and the bladder was hypoechoic due to the use of improper gain. The average length of the right kidney was 4.73±0.50 cm and the left kidney was 4.08±0.26 cm. The bladder wall thickness was 1.3±0.31 mm. Imaging of the bladder showed changes in wall size and echogenicity. Prostate ultrasound results obtained an average length of 2.68±0.45 cm, width 1.69±0.35 cm. The results of this study can be used as a reference for normal data on organ position, organ structure, and organ echogenicity of the spleen, stomach, liver, kidney, bladder, and prostate in piglets.
Kejadian nematodosis gastrointestinal pada monyet ekor panjang (Macaca fascicularis) di Pusat Studi Satwa Primata LPPM IPB Rosyid, Bahaudien; Darusman, Huda Shalahudin; Retnani, Elok Budi
Current Biomedicine Vol. 1 No. 1 (2023): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.1.33-45

Abstract

Background: The long-tailed macaque (Macaca fascicularis) is often used as a model animal in biomedical research. Nematodosis is common among non-human primates. Gastrointestinal nematodosis may affect study results or be a potential source of transmission to humans (zoonosis). Objective: The study aimed to identify the type, calculate the degree of infection, and the prevalence of gastrointestinal nematode worms in long-tailed macaques in captivity at the Primate Research Center, Institute for Research and Community Service (PSSP LPPM) IPB University. Methods: Faeces samples from 24 long-tailed macaques were examined using the McMaster and Kato-Katz simple flotation methods. Results: Nine (37.5%) of the 24 samples were infected by trichurid (20.8%), ascarid (12.5%), and strongylid (4.2%), each with an average degree of infection 833.3 ± 923.8, 32 ± 27.7, and 12 ± 17 eggs per gram of faeces (EPG), respectively. The prevalence of nematodosis in long-tailed macaques based on age group and sex was in infants 50%, children 50%, adolescents 16.7%, adults 33.3%, males 33.3%, and females 41.7%. Conclusion: The incidence and degree of gastrointestinal nematodosis infection in long-tailed macaques showed a moderate to low level. These results can be considered in maintenance management, biomedical research, and mitigation of potential transmission risk to humans.
Kuantifikasi Kepucatan Konjungtiva menggunakan Sensor TCS 34725 pada Domba Model Anemia Haemonchosis Arif, Ridi; Retnani, Elok Budi; Satrija, Fadjar; Fitri, Raudhatul; Santriagung, Mochamad Alfinanda; Nurzuliana, Dina
Acta VETERINARIA Indonesiana Vol. 11 No. 1 (2023): Maret 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.1.1-8

Abstract

Infeksi Haemonchus contortus (haemonchosis) sering menimbulkan gejala anemia karena sifatnya yang menghisap darah. Penelitian ini bertujuan menilai tingkat kepucatan konjungtiva mata domba menggunakan scanner berbasis sensor TCS 34725. Alat scanner konjungtiva dibuat menggunakan Arduino Uno sebagai mikrokontroler dan dilengkapi dengan sensor kecerahan, sensor jarak, dan layar LCD 16 x 2. Domba percobaan dibuat mengalami anemia sebagai model dari kondisi haemonchosis anemia dan dibagi ke dalam kelompok D1 dan D2. Selanjutnya dilakukan pengambilan data warna konjungtiva menggunakan scanner dan sampel darahnya untuk diperiksa jumlah sel darah merah (RBC), nilai hematokrit (PCV), Hb, dan indeks eritrositnya. Pengambilan sampel dilakukan pada hari ke 0, 3, dan 6. Kondisi anemia buatan berhasil tercapai dengan dibuktikannya warna konjungtiva yang terlihat semakin pucat pada hari ke-3 dan 6. Hasil pengukuran scanner menunjukkan rata-rata brightness di dalam kandang selama pengambilan data sensor adalah 433.56±122.62 dengan nilai RGB yang bervariasi pada kelompok D1 dan D2. Seiring dengan kondisi warna yang semakin pucat terlihat nilai Red dan Green juga menurun sedangkan nilai Blue tidak. Hasil pemeriksaan darah menunjukkan bahwa nilai RBC, Hb, PCV, dan indeks eritrosit pada D1 terlihat menurun pada hari ke 3 dan mulai recovery pada hari ke-6 meski warna konjungtiva masih terlihat pucat. Pada kelompok D2, hasil pemeriksaan darah menunjukkan nilai yang terus menurun sampai hari ke-6 seiring dengan konjungtiva yang bertambah pucat. Hubungan yang terlihat kuat adalah nilai Red hasil dari alat scanner dengan nilai Hb hasil pemeriksaan darah. Simpulan dari penelitian ini adalah sensor TCS 34725 dapat digunakan untuk mengkuantifikasi kepucatan konjungtiva dan mampu untuk menduga kondisi anemia pada domba karena memiliki korelasi dengan kadar Hb di dalam darah.
RELIABILITY OF AMYLOID DETECTION IMMUNOASSAY KITS FOR ALZHEIMERS DISEASE SCREENING: A PRELIMINARY STUDY Putri, Indah Aprianti; Ningrum, Emilna Mega; Noviana, Rachmitasari; Mariya, Silmi; Saepuloh, Uus; Retnani, Elok Budi; Hamdan, Muhammad; Nugraha, Jusak; Darusman, Huda Shalahudin
Jurnal Kedokteran Hewan Vol 18, No 3 (2024): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i3.31109

Abstract

Alzheimers Disease is a progressive neurodegenerative disease that damages the brain and is part of dementia. Alzheimers dementia is caused by various factors that until now the proper therapy is uncertain. Kit production as a diagnostic tool to support the development of screening for Alzheimers disease is a strategic effort. Screening based on detection with Enzyme-Linked Immunosorbent Assay (ELISA) can work effectively and accurately to optimize a specific antibody material or monoclonal antibody to the A42 peptide. This study aimed to research the reliability of the amyloid detection kit which was developed as an alternative screening test for Alzheimers disease compared to commercially available kits. Based on the results and interpretation of inter and intra-assay coefficients, the in-house ELISA kit has comparable A42 detection to the commercially available ELISA kit
ULTRASOUND IMAGING OF THE SPLEEN, STOMACH, LIVER, KIDNEYS, BLADDER, AND PROSTATE IN PIGLETS (Sus scrofa domestica) Gunanti, Gunanti; Khairunnisa, Carina; Annisa, Fadhilah Nur; Retnani, Elok Budi; Amin, Akhmad Arif; Rahmiati, Dwi Utari
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.21784

Abstract

This study aims to describe ultrasonographic imaging of the spleen, stomach, liver, kidney, bladder, and prostate in piglets (Sus scrofa domestica). Ultrasonography was performed on six male piglets aged 1 month with a body weight of 4-8 kg. Ultrasonography of the spleen, stomach, liver, urinary bladder, and prostate was observed in the dorsal-recumbency position, and the kidney was observed in the lateral-recumbency position. The ultrasound examination used the Chison EBit 60, which is a linear type transducer with a frequency of 9 MHz, transverse and sagittal positions, and a gain of about 55-80. Ultrasonography of the spleen showed that the shape of the spleen head varied in each individual. The results of gastric ultrasonography showed the gastric lining clearly and the similarity of the position, shape, structure, echogenicity, and size of the stomach. The results of liver ultrasonography showed that the echogenicity of the liver tended to be hyperechoic, the kidneys were hyperechoic, and the bladder was hypoechoic due to the use of improper gain. The average length of the right kidney was 4.730.50 cm and the left kidney was 4.080.26 cm. The bladder wall thickness was 1.30.31 mm. Imaging of the bladder showed changes in wall size and echogenicity. Prostate ultrasound results obtained an average length of 2.680.45 cm, width 1.690.35 cm. The results of this study can be used as a reference for normal data on organ position, organ structure, and organ echogenicity of the spleen, stomach, liver, kidney, bladder, and prostate in piglets.
Co-Authors A.S. Satyaningtijas Ainul Khadija Saleema Amalan Tomia Annisa, Fadhilah Nur Berlin Berliana Anugrah Carina Khairunnisa David Anwar Desi Nupremani Dermawan Desrayni Hanadhita DEWI APRI ASTUTI Dhea Ardhina Krisdamaiyanti Dwi Utari Rahmiati Erna Suzanna Fadhilah Nur Annisa Fadjar Satrija Felicia Rizal Putri Fitri, Raudhatul Gunanti . HERA MAHESHWARI Hilman Rizki Pangestu Huda Shalahudin Darusman Irwan Oktoni Januarius Jimi Jefri Naldi Jusak Nugraha Khairunnisa, Carina koekoeh santoso Lily Zalizar Muhamad Cahadiyat Kurniawan Muhammad Falikhul Musyaffa' Muhammad Hamdan Nadira Fadilah Nanis Nurhidayah Niken Ulupi Ningrum, Emilna Mega Noviana, Rachmitasari Noviriliensi Hartika Nurzuliana, Dina Putri, Indah Aprianti R Irpan Pahlepi Ridi Arif Risa Tiuria Rizal Arifin Akbari Rizky Diyu Purnama Rosyid, Bahaudien Rudy Rudy Santriagung, Mochamad Alfinanda SILMI MARIYA Simon He SINGGIH H. SIGIT Singgih Harsoyo Sigit sri murtini . Sumiati Tomia Supan Kusumamihardja Susi Soviana Upik Kesumawati Hadi UUS SAEPULOH Virgilius Martin Kelake Kedang Yulvian Sani Yusuf Ridwan Zulfikri Mustakdir