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Isolasi Dan Identifikasi Senyawa Antioksidan Dari Buah Kasturi (Mangifera casturi Kosterm.) Sutomo Sutomo; Hadi Azhari; Arnida Arnida; Fadlilaturrahmah Fadlilaturrahmah; Rahmat Yunus
Jurnal Pharmascience Vol 4, No 2 (2017): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v4i2.5778

Abstract

ABSTRAK Antioksidan berfungsi untuk menetralisir radikal bebas, sehingga dapat mencegah dan melindungi terjadinya kerusakan tubuh yang dapat menyebabkan timbulnya penyakit degeneratif. Salah satu tanaman endemik Kalimantan Selatan yang teridentifikasi mengandung antioksidan alami yaitu kasturi (Mangifera casturi Kosterm.). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa antioksidan dari buah M. casturi. Ekstraksi dilakukan dengan metode maserasi. Pemisahan dan pemurnian dilakukan dengan KLT , KCV, dan kromatografi kolom gravitasi. Identifikasi senyawa dengan spektrofotometri UV-Vis dan FTIR serta uji kualitatif antioksidan dan aktifitas kuantitatif antioksidan isolat. Ekstraksi 2500 gram serbuk buah M. casturi dengan metanol diperoleh 840,69 gram ekstrak kental berwarna coklat. Fraksinasi 60 gram ekstrak metanol dengan fraksi etil asetat menghasilkan 8,3 gram ekstrak kering. Fraksi etil asetat difraksinasi kembali menggunakan KCV dengan eluen n-heksana : etil asetat (9:1; 8:2; 7:3; 6:4; 5:5; 4:6; 3:7) v/v, etil asetat : metanol (5:5 v/v), 100% metanol diperoleh fraksi A, B, C, D, E, F, G, dan H. Fraksi H dipilih untuk di isolasi menggunakan kromatografi kolom gravitasi dengan eluen n-heksana : etil asetat : metanol (5:3:0,5; 5:4:1; 5:4:2; 5:6:2; 5:6:2,5) v/v dan 100% metanol. Uji kualitatif KLT menunjukkan isolat H-7 mengandung senyawa antioksidan dengan pereaksi DPPH. Aktivitas antioksidan isolat H-7 memiliki nilai IC50¬ sebesar 4,61±0,57 ppm. Analisis isolat H-7 dengan UV-Vis menghasilkan puncak pada λ 274,8 nm (pita I) dan λ 218 nm (Pita II). Analisis FTIR menunjukkan gugus fungsi dari isolat H-7 yaitu -OH, C-H aromatik, C-H alifatik, C=O, C=C aromatik, dan C-O. Kata Kunci : Antioksidan, buah M. casturi, DPPH, fraksi etil asetat, Isolasi
Studi Farmakognostik dan Uji Parameter Nonspesifik Ekstrak Metanol Kulit Batang Kasturi (Mangifera casturi Kosterm.) Sutomo Sutomo; Nadya Agustina; Arnida Arnida; Fadilaturrahmah Fadilaturrahmah
Jurnal Pharmascience Vol 4, No 1 (2017): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v4i1.5761

Abstract

ABSTRAK Kasturi (Mangifera casturi Kosterm.) merupakan salah satu tumbuhan endemik Kalimantan Selatan. Kulit batang M. casturi mengandung beberapa golongan senyawa yang berpotensi sebagai obat. Penelitian ini bertujuan untuk memberikan data hasil analisis farmakognostik, batas-batas maksimal kandungan senyawa tertentu dan profil kromatogram kandungan kimia ekstrak metanol kulit batang M. casturi. Analisis farmakognostik terhadap tumbuhan meliputi morfologi, anatomi, dan identifikasi kandungan kimia. Batasan maksimal kandungan senyawa tertentu berdasarkan parameter non spesifik meliputi kadar air, kadar abu total, kadar abu tidak larut asam, total bakteri dan kapang, dan kadar Pb dalam ekstrak. Karakteristik tumbuhan M. casturi yaitu memiliki batang berwarna coklat tua dengan permukaan kasar dan bergetah, daun berwarna hijau, berbentuk lancet, kulit buah matang berwarna coklat keunguan, daging buah berwarna kuning terang hingga jingga, berbau khas, berasa manis agak asam dan banyak mengandung serabut. M. casturi mengandung senyawa alkaloid, flavonoid, terpenoid, fenol dan saponin yang dibuktikan berdasarkan uji identifikasi kimia dan analisis secara KLT. Pengujian parameter non spesifik ekstrak metanol kulit batang M. casturi secara berturut-turut yaitu, kadar air 12,5±0,7%; kadar abu total 1,0±0,5%; kadar abu tidak larut asam 0,67±0,28%; tidak terdeteksi adanya pertumbuhan bakteri pada ekstrak kulit batang M. casturi; sedangkan total kapang yaitu 250 koloni/g; dan kadar Pb yaitu 3 mg/kg. Kata Kunci: Kasturi, analisis, farmakognostik, Mangifera casturi, parameter non spesifik ABSTRACT Kasturi (Mangefera casturi Kosterm.) is one of the endemic plants of South Kalimantan. M. casturi bark contain several group of compounds potential to the treatment. This research aims to provide data analysis results pharmacognostic, maximum limits of certain compounds and profile chromatogram of chemical content of methanol extract from M. casturi bark. Pharmacognostic analysis of the plant include morphology, anatomy, and the identification of compounds. The maximum limits of certain coumpounds by non-specific parameters include water content, total ash, ash content insoluble in acid, total bacteria and fungi, and Pb content in the extract. M. casturi characteristic is having dark-brown stems with roughly surface and sticky, green leaves, lancet-shapes, fruit skin has a purplish brown colour, flesh of fruit has a bright yellow till orange colour, distinctive smell, slightly sour sweet taste, and contains lots of fiber. M. casturi bark contains alkaloids, flavonoids, terpenoids, phenols, and saponins that has been proved by chemical identification test and analysis of TLC. Non-specific parameter testing about methanol extract of M. casturi bark consecutively given, water content 12,5±0,7%; total ash 1,0±0,5%; ash content insoluble in acid 0,67±0,28%; undetected any bacterial contamination in the extract of M. casturi bark; while the total mold contamination is 250 colonies/g; and Pb contents is 3 mg/kg. Key words: Kasturi, pharmacognostic analysis, Mangifera casturi, non-specific parameter
AKTIVITAS ANTIPLASMODIUM In vitro DAN IDENTIFIKASI GOLONGAN SENYAWA DARI EKSTRAK ETANOL BATANG MANURAN (Coptosapelta tomentosa Valeton ex K.Heyne) ASAL KALIMANTAN SELATAN Arnida Arnida; Eka Rahmawaty Sahi; Sutomo Sutomo
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 2 No 2 (2017): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.024 KB) | DOI: 10.36387/jiis.v2i2.119

Abstract

Resistensi Plasmodium terhadap obat malaria mengakibatkan kegagalan pengobatan. Hal ini merupakan ancaman terlebih belum ditemukannya obat alternatif yang efektif untuk melawan resistensi.  Oleh karena itu ketersediaan antimalaria baru sangat diperlukan untuk melawan resistensi. Pencarian obat baru terus dilakukan melalui berbagai cara termasuk eksplorasi dan pengembangan bahan alam. Pemilihan bahan alam biasanya berdasarkan penggunaan secara empiris oleh masyarakat. Tanaman Manuran (Coptosapelta tomentosa Valeton ex K.Heyne) digunakan oleh masyarakat di Kotabaru Kalimantan Selatan untuk mengobati malaria. Penelitian yang dilaksanakan adalah melakukan uji aktivitas antiplasmodium in vitro dengan menentukan nilai konsentrasi penghambatan (IC50­­) dan melakukan identifikasi golongan senyawa dari ekstrak etanol batang C. tomentosa Valeton ex K.Heyne. Berdasarkan identifikasi golongan senyawa kimia dengan skrining fitokimia dan Kromatografi Lapis Tipis menunjukkan adanya senyawa flavonoid, terpenoid, saponin, tanin dan antrakuinon pada batang C. tomentosa Valeton ex K.Heyne. Ekstrak etanol batang C. tomentosa Valeton ex K.Heyne mempunyai aktivitas antiplasmodium in vitro tergolong aktif dengan IC50 45,864 ± 0,76 µg/mL.
STANDARDISASI BUAH CABE RAWIT HIYUNG (Capsicum frutescens L.) ASAL TAPIN KALIMANTAN SELATAN Sutomo Sutomo; Aulea Rahmawati; Muhammad Ikhwan Rizki
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 2 No 2 (2017): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.304 KB) | DOI: 10.36387/jiis.v2i2.121

Abstract

Cabe rawit hiyung (Capsicum frutescens L.) merupakan cabe rawit lokal khas Tapin, Kalimantan Selatan. Penelitian ini bertujuan untuk menentukan nilai parameter standardisasi simplisia dan ekstrak buah cabe rawit hiyung. Standardisasi dilakukan dengan menetapkan nilai parameter spesifik dan non spesifik simplisia dan ekstrak dari tiga tempat tumbuh yang berbeda yang meliputi uji organoleptik simplisia, uji mikroskopik, kadar sari larut etanol, kadar sari larut air, susut pengeringan, kadar abu total simplisia, kadar abu tidak larut asam simplisia, cemaran logam berat, pemerian ekstrak, rendemen, skrining fitokimia, pola kromatografi, penentuan kadar flavonoid total, kadar air, kadar abu total ekstrak, dan kadar abu tidak larut asam ekstrak. Hasil uji  standardisasi simplisia dari ketiga desa diperoleh rata-rata berupa serbuk, berwarna kemerahan, rasa sangat pedas, berbau khas, terdapat sel epidermis, hipodermis dan parenkrim mesokarp, kadar sari larut etanol 19,55 ± 1,07%, kadar sari larut dalam air 32,33 ± 2,03%, susut pengeringan 2,22 ± 0,51%, kadar abu total 4,11 ± 0,44%, kadar abu tidak larut asam 0,14 ± 0,01%, cemaran logam berat Pb 2,00 mg/kg dan Cd 4,00 mg/kg. Hasil uji standardisasi ekstrak dari ketiga desa diperoleh rata-rata berupa ekstrak kental, berwarna coklat, rasa sangat pedas, berbau khas, dengan rendemen sebesar 19,19 ± 1,03%, kandungan kimia yang terkandung adalah alkaloid, flavonoid, fenolik, saponin, pola kromatogram dengan nilai Rf 0,18; 0,60; 0,98, kadar flavonoid total yang paling besar yaitu desa Sungai Rutas 0,339%, kadar air 11 ± 0,67%, kadar abu total 7,96 ± 0,80%, dan kadar abu tidak larut asam 0,61 ± 0,05%.
SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN RACUN AYAM (Brucea javanica [L.] Merr.) ASAL KALIMANTAN SELATAN Sutomo Sutomo; Fahriah Fahriah; Arnida Arnida
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 6 No 1 (2021): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.647 KB) | DOI: 10.36387/jiis.v6i1.607

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Racun ayam is one of the plants which has several activities in its fruits, one of which is an antibacterial. This study aims to determine the antibacterial activity of ethanol extract of racun ayam leaves. The extraction was done by maceration method using ethanol as a solvent. Antibacterial activity assay was carried out on S. aureus, E. coli, and P. aeruginosa bacteria by disk diffusion method at the concentrations of 80, 100, and 120 ppm. The results showed that the ethanol extract of B. javanica leaves had the greatest inhibition zone on E. coli with inhibition zone diameter values ​​at 80, 100, and 120 ppm were 11.7 mm, 14.5 mm and 19.67 mm respectively. Inhibition zones in S. aureus could only be seen at 100 and 120 ppm which had inhibition zone diameters at 1.17 mm and 2.17 mm respectively, whereas in P. aeruginosa they did not have any inhibitory zone values. The results of statistical analysis showed a significance of the inhibition zone diameter values ​​of E. coli bacteria starting at 80 ppm with a sig value of 0.034. Due to a sig value was <0.05, it was categorized as significantly different from the negative control. The bacteria of S. aureus which had ​​<0.05 for sig values only found on 100 ppm and 120 ppm. Bacteria P. aeruginosa which had sig value >0.05 was not significantly different at all concentrations
Heme Polymerization Inhibitory Activity And Phytochemical Screening Of Ethyl Acetate Fraction In Manuran (Coptosapelta tomentosa Valeton ex K. Heyne) Stem Arnida; Siti Humairah Z.A; Sutomo; Fadlillahturrahmah
Jurnal Jamu Indonesia Vol. 6 No. 1 (2021): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v6i1.165

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The native Indonesian plant that is empirically used as an antimalarial agent is manuran (Coptosapelta tomentosa Valeton ex K. Heyne). This study aims to determine chemical compound and heme polymerization inhibitory activity of ethyl acetate fraction of C. Tomentosa stem based on IC50 value. The method identification of chemical compound used tube test, and the method of heme polymerization inhibitory activity was Basilico through in vitro method. The results of chemical compound identification of the ethyl acetate fraction of C. Tomentosa showed the presence of flavonoids, terpenoids, saponins, tannins, and anthraquinones. The average percentages of heme polymerization inhibitory activity of ethyl acetate fraction of C. Tomentosa stem from concentration 20; 10; 5; 2.5; 1.25; 0.625; 0.3125 mg / mL were 98.507; 97,872; 96,407; 93,560; 88,419; 80,680; and 45.467%.The averages of IC50 of ethyl acetate fraction and chloroquine diphosphate were 0.24 &plusmn; 0.018 mg/mL and 0.214 &plusmn; 0.012 mg/mL. This shows that the ethyl acetate fraction of C. Tomentosa stem has heme polymerization inhibitory activity. The result of the independent sample t-Test obtained the significance value of 0.111 (p more than 0.05) that there was no significant difference. It means that the ethyl acetate fraction of C. Tomentosa stem has heme polymerization inhibitory activity as well as chloroquine diphosphate. This suggests the potentiation of the methyl acetate fraction of the stem C. Tomentosa as anti-malarial.
Formulasi dan Evaluasi Sediaan Masker Peel-Off dari Ekstrak Buah Kasturi (Mangifera casturi Kosterm.) dengan Variasi Konsentrasi PVA Sutomo Sutomo; Nhaya Riskita; Mia Fitriana
Jurnal Pharmascience Vol 10, No 1 (2023): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v10i1.15569

Abstract

Kasturi (Mangifera casutri Kosterm.) merupakan salah satu tumbuhan endemik dari Kalimantan yang telah terbukti memiliki senyawa yang berpotensi sebagai antioksidan alami. Ekstrak buah tumbuhan M. casturi dapat dibuat menjadi salah satu bentuk sediaan masker peel-off dengan memanfaatkan polivinil alkohol (PVA) untuk film-forming agent. Sediaan masker peel-off mampu merileksasikan terhadap otot wajah, melembabkan, dan juga dapat membersihkan kulit wajah serta efektif dalam menghilangkan sel kulit yang mati. Tujuan dari penelitian ini adalah untuk menetapkan sifat fisik apa saja yang dapat dipengaruhi oleh adanya variasi konsentrasi PVA. Metode yang digunakan pada penelitian ini adalah metode eksperimental. Variasi konsentrasi PVA yang digunakan pada penelitian ini yaitu: formula 1, 2, dan 3 secara berturut-turut adalah 8%, 10%, dan 12%. Evaluasi yang dilakukan meliputi uji organoleptik, homogenitas, pH, daya lekat, daya sebar, waktu kering, dan uji hedonik. Evaluasi yang dilakukan terhadap formula 1, 2, dan 3 menegaskan bahwa dengan adanya variasi konsentrasi dari PVA menghasilkan formula yang berbeda secara signifikan baik dari hasil pengujian pH, daya lekat, daya sebar, dan waktu kering sediaan. Dari hasil penelitian tersebut dapat disimpukan bahwa dengan bervariasinya konsentrasi PVA yang digunakan dapat mempengaruhi terhadap sifat organoleptis, pH, daya lekat, daya sebar, serta waktu kering sediaan. Kata Kunci: Masker Peel-off, Buah Kasturi, Mangifera casturi, Polivinil Alkohol, Formulasi  Casturi fruit (Mangifera casturi Kosterm.) is one of the endemic plants of South Kalimantan which contains natural antioxidants compound. The casturi fruit extract was formulated into a peel-off mask preparation using polyvinyl alcohol (PVA) as a film-forming agent. The peel-off mask has several benefits, including being able to relax facial muscles, cleanse and moisturize facial skin and effective in removing dead skin cells. This research aimed to determine the physical characteristics that are influenced by the PVA concentration variations. The used PVA concentration variations are formula 1 (8%), formula 2 (10%) and formula 3 (12%). The conducted evaluations are the organoleptic tests, homogeneity, pH, adhesion, spread ability, dry time, and hedonic tests. The evaluation results show that there are significant differences in the pH test, adhesion, spread ability and dry time of the preparations. The conclusion of this research is that the PVA concentration variations affected the organoleptic test, pH, adhesion, dispersion and dry time of the casturi fruit extract peel-of mask.
Simplicia and Extracts Standardization from Jualing Leaves (Micromelum minutum Wight & Arn.) from South Kalimantan Sutomo Sutomo; Herwina Dita Lestari; Arnida Arnida; Agung Sriyono
Borneo Journal of Pharmacy Vol. 2 No. 2 (2019): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v2i2.898

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Jualing (Micromelum minutum Wight & Arn.) is a plant from South Kalimantan that has the potential to be developed as natural medicine. This study aims to establish standardization which includes specific and non-specific parameters of M. minutum leaf simplicia and extracts from three growing locations, namely Banua Botanical Garden, Sultan Adam Forest Park, and Forest Areas with Specific Objects of Rantau, South Kalimantan. The method of setting standardization parameters refers to the Indonesian Herbal Pharmacopoeia and General Standard Extracts Parameters. Organoleptic standardization of simplicia is brownish-green, has a distinctive odor, has a bitter and slightly spicy taste. Microscopic observations showed the presence of stomata, cell walls, cytoplasm, calcium oxalate crystals, upper epidermis, palisade tissue, spongy tissue, cortex, xylem, phloem, lower epidermis, and trichomes. Water content test showed the results of 21.9-22.07%; ethanol extract content of 12.87-13.17%; drying losses 4.64-4.84%; total ash content of 6.04-6.14%; acid insoluble ash content 1.13-1.19%; Pb levels of 0.022-0.025 mg/kg; Cd levels of 0.017-0.020 mg/kg; and Hg levels <0.0004 mg/kg. Extract standardization shows thick green-black, thick-smelling extract and bitter taste. Micromelum minutum leaves contain alkaloids, flavonoids, saponins, terpenoids, tannins, anthraquinones, phenols, and glycosides. Thin Layer Chromatography profiles show the similarity of chemical compounds in each growth location. Other parameters are extract yield of 8.08-8.32%; moisture content of 6.07-6.27%; total ash content of 4.22-4.27%; and acid insoluble ash content 1.11-1.12%. All standardization parameters meet the requirements in the Media Materia Indonesia and the Republic of Indonesia Drug and Food Control Center.
Pharmacognostic Study and Antioxidant Activity of Mundar (Garcinia forbesii King.) leaves from Banua Botanical Gardens of South Kalimantan Sutomo Sutomo; Dina Noor Kamali; Arnida Arnida; Normaidah Normaidah; Agung Sriyono
Borneo Journal of Pharmacy Vol. 3 No. 4 (2020): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v3i4.1541

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Mundar (Garcinia forbesii King.) is a plant from South Kalimantan. This plant has chemical contents that have potential as medicine. The purpose of this study is to provide a pharmacognostic picture of a specific, nonspecific and determine the antioxidant activity of G. forbesii leaves. Specific parameters include organoleptic, microscopic testing, thin-layer chromatography profiles, and phytochemical screening. Nonspecific parameters include total ash content, acid insoluble ash content, drying loss, water-soluble extract content, and ethanol-soluble extract content. Antioxidant activity was determined by the DPPH method based on IC50 values. Specific parameter test results are green powder, characteristic odor, and sour taste. Microscopic tests showed stomata, upper epidermis, lower epidermis, cell walls, xylem, phloem, palisade tissue, spongy tissue, and cuticles. Garcinia forbesii leaves contain alkaloids, flavonoids, phenols, tannins, and steroids. The TLC profile showed good separation of polar eluents, and yellow spots appeared after spraying DPPH reagents. The non-specific parameter test is the total ash content of 6.52±0.1%; acid insoluble ash content 1.06±0.08%; drying shrinkage 6.43±0.38%; water-soluble extracts 34.3±0.3%; and ethanol-soluble extracts 23.47±0.35%. Ethanol extract of G. forbesii leaves has antioxidant activity with IC50 of 65.7 ppm. Pharmacognostic study fulfills the requirements, and G. forbesii leaves extract has strong antioxidant activity.
Ethnobotanical Study and Phytochemical Screening of Medicinal Plants Used by Local People in Belangian Village, South Kalimantan Sutomo Sutomo; Vita Vitriana Awaliyah; Arnida Arnida
Borneo Journal of Pharmacy Vol. 5 No. 1 (2022): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v5i1.2717

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The local people of Belangian Village are people of the Banjar ethnic group who utilize plants in the surrounding environment for medicinal purposes. This study aims to determine the number of plants, parts of plants that can be used, methods of application, methods of preparation, and phytochemical screening of plants with medicinal properties in Belangian Village. This study employed the descriptive-explorative method. The technique used for data collection was an interview with a semi-structured questionnaire. Respondents of this study were “Pananamba” who were selected by using the Purposive sampling technique. Phytochemical screening was carried out on plants with no previously known scientific names and which had never been tested before. The results showed 17 families and 31 species of plants with medicinal properties. Empirically, the plants were used as medicines for cough, bloody urine, diabetes, cancer, sprue, ulcer, hypertension, skin diseases, nausea, diarrhea, coronary heart disease, sinusitis, kidney stones, cleansing kidneys, menstrual pain, itchy eyes, paralysis, bone pain, increasing stamina, antibiotic, reducing body odor, wounds, bleeding, worm diseases, increasing appetite, boosting the immune system, and yellow fever. The most frequently used part of the plants were the leaves (45%), the most widely used method of preparation was boiling (52%), the most commonly used method of application was by drinking (81%). Phytochemical screening was conducted on three plants: Asam daun, Lukun, and Ulur-ulur. The results of phytochemical screening revealed the presence of quinones, saponins, and terpenoids in Asam daun, saponins in Lukun, and terpenoids in Ulur-ulur.