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Fraksi Kloroform Ekstrak Buah Mentimun (Cucumis sativus L.) Sebagai Anti Bakteri terhadap Staphylococcus epidermidis Viogenta, Pratika; Samsuar, Samsuar; Utama, Ahmad Ferry Yeriza
Jurnal Kesehatan Vol 8, No 2 (2017): Jurnal Kesehatan
Publisher : Politeknik Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.086 KB) | DOI: 10.26630/jk.v8i2.410

Abstract

The use of antibiotics in the community massively trigger resistance to bacteria, to that need for research on other alternatives, especially herbal medicine as an antibacterial. One of the medicinal plants are cucumbers that are widely available in Indonesia. The purpose of this study was to scientifically prove the antibacterial activity of chloroform fraction cucumber extract against Staphylococcus epidermidis. Extraction cucumber done by maceration, then extract fractionated with chloroform. The fraction was used the concentration of 5%, 10%, 15%, 20% and 25%. Then tested the antibacterial power and determination of the MIC. Results of test antibacterial of the extract chloroform of cucumber against S. epidermidis showed a zone of inhibition at concentrations of 5%, 10%, 15%, 20%, 25% diameter respectively 5.86 mm, 6.5 mm, 7.05 mm, 7.43 mm, and 9.92 mm, in addition to it tested the determination of minimum inhibitory concentration, minimum inhibitory concentrations of chloroform fraction extract cucumber fruit against S. epidermidis is at a concentration of 3%. The results obtained from the culture media growth on NA which means the fraction of the chloroform extract of cucumber in 3% is bacteriostatic.
The Kualitas Kacang Tanah (Arachis hypogeae L.) Kefir dengan Variasi Konsentrasi Starter Ragi dan Lama Fermentasi Pratika Viogenta; Nani Kartinah; Amalia Khairunnisa; Fathur Rahman
Biota Vol 7 No 2 (2021): Jurnal Biota 2021
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v7i2.8147

Abstract

One of the fermented milk products, namely kefir, is increasingly popular because it has many health benefits. Peanut juice has a high enough protein content that it can be used as a substitute for animal milk. The purpose of this study was to determine the quality of peanut kefir with variations in the concentration of ragi tape inoculum and fermentation time. This research method used 3 variations in the concentration of tape yeast (1, 2 and 4%) and long fermentation time (24, 48 and 72 hours). The results showed that the highest total LAB was in 4% ragi tape inoculum with a fermentation time of 24 hours (4.4x108 cells/mL) and the lowest was in 4% tape yeast inoculum with a fermentation time of 72 hours (9.8x107 cells/mL) and total yeast between 1x104 cells/mL - 3x105 cells/mL) and alcohol produced <1%. Total acid obtained between 6% - 17.6%. The increase in total acid is proportional to the decrease in pH. The pH of the peanut kefir medium was between 3.44 - 4.12. Peanut kefir with tape yeast inoculum meets the standard requirements for fermented milk and can replace milk kefir.
Fraksi Kloroform Ekstrak Buah Mentimun (Cucumis sativusL.) Sebagai Anti Bakteri Terhadap Staphylococcus epidermidis Samsuar User; Pratika Viogenta; Ahmad Ferry Yeriza Utama
Inovasi Pembangunan : Jurnal Kelitbangan Vol 5 No 02 (2017): August 2017
Publisher : Balitbangda Provinsi Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.317 KB)

Abstract

The use of antibiotics massively in the community triggered resistance to bacteria insoas to need researchforother alternatives, especially herbal medicine as an antibacterial. One of the medicinal plants was cucumber fruit that was widely available in Indonesia. The purpose of this study was to scientifically prove the antibacterial activity of chloroform fraction of cucumber fruit extract against Staphylococcus epidermidis. The extraction of the cucumber fruit was done through maceration with ethanol 70 % followed with the extract was fractionated with chloroform. The fraction was performed in the chloroform at the concentration of 5, 10, 15, 20 and 25 %. The fraction was tested for the antibacterial strength and determined the Minimum Inhibitory Concentration (MIS). The results of antibacterial strength of the extract chloroform of cucumber fruit againstS.epidermidisshowed a zone of inhibition at concentration of 5, 10, 15, 20, 25 %each having diameter of 5.86, 6.5, 7.05, 7.43, and 9.92 mm, respectively. In addition to calculate the concentration, the research also determined the MIS. The MIS of chloroform fraction extract cucumber fruit against S. epidermidis wasattheconcentration of 3 %. The result obtained from the growth on the Nutrient Agar culture media indicated that the fraction of the chloroform extract of cucumber in 3% is bacteriostatic.
Aktivitas Antibakteri Ekstrak Akar Ceplukan (Physalis angulata L.) Terhadap Staphylococcus epidermidis dan Pseudomonas aeruginosa Pratika Viogenta; Lilik Koernia Wahidah; Ika Harum Saputri
JFL : Jurnal Farmasi Lampung Vol. 6 No. 2 (2017): JFL: Jurnal Farmasi Lampung
Publisher : Fakultas MIPA Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.225 KB) | DOI: 10.37090/jfl.v6i2.20

Abstract

Root ceplukan (Physalis angulata L.) is one of the medicinal plants that contain several active compounds that are antibacterial. This study aims to prove the antibacterial activity of root ceplukan extract againsts Staphylococcus epidermidis bacteria and Pseudomonas aeruginosa bacteria. Root ceplukan was extracted by maceration with ethanol 70%. Root root extracts of phytochemical screening include flavonoids, alkaloids and saponins. The ceplukan root extract was then tested against S. epidermidis and P. aeruginosa bacteria using wells method with concentrations of 20%, 40%, 60%, 80%, 100%, aquades as a negative control and amikacin as a positive control. Inhibitory zone diameter with each concentration for P. aeruginosa bacteria, among others, negative control = 0 mm, positive control = 21,623 mm, 100% = 16.9 mm, 80% = 15.05 mm, 60% = 13.703 mm, 40% = 12.02 mm, 20% = 12.02 mm and S. epidermidis bacteria have inhibitory zone at positive control of 21,623 mm. The results of this study showed that root extract ceplukan proved to have inhibition zone against bacteria P. aeruginosa at all concentrations but has no inhibition zone against S. epidermidis bacteria. Root ceplukan extract has a minimum inhibitory concentration of 14% and a minimum bacterisid concentration of 20%, against P. aeruginosa bacteria. The results of phytochemical analysis showed that root ceplukan positively contain flavonoids, alkaloids and saponins are suspected as antibacterial compounds. Keywords : Antibacterial, Staphylococcus epidermidis, Pseudomonas aeruginosa, Ceplukan root extract
Optimasi Zeolit Alam Lampung (ZAL) Sebagai Penstabil Sediaan Antibakteri Esktrak Buah Mengkudu (Morinda citrifolia L.) Laila Susanti; Lilik Koernia Wahidah; Pratika Viogenta
JFL : Jurnal Farmasi Lampung Vol. 7 No. 2 (2018): JFL: Jurnal Farmasi Lampung
Publisher : Fakultas MIPA Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.262 KB) | DOI: 10.37090/jfl.v7i2.54

Abstract

Noni fruit extract has been widely known as an antibacterial, but plant extracts are easily degraded by light, pH and temperature, thus reducing the antibacterial activity, zeolite is added to maintain the stability of the noni extract. This study uses an experimental method that is by comparing the inhibition zones that occur between noni fruit extract and noni + ZAL fruit extract and to test the stability of the preparation, the sample is heated at 40oC and then tested for antibacterial activity again. The statistical test results show the value of Asymp. Sig (0.003)
FRAKSI ETANOL RIMPANG JERINGAU (Acorus calamus L.) SEBAGAI ANTIBAKTERI RERHADAP Staphylococcus aureus DAN Escherichia coli Pratika Viogenta; Nopiyansyah Nopiyansyah; Fitri Fitri
JFL : Jurnal Farmasi Lampung Vol. 7 No. 2 (2018): JFL: Jurnal Farmasi Lampung
Publisher : Fakultas MIPA Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.026 KB) | DOI: 10.37090/jfl.v7i2.60

Abstract

Acorus calamus L. (jeringau) rhizomes are one of the medicinal plants used as traditional medicine in Indonesia. This plant were used as an antibacterial. The purpose of this research were to prove the inhibition jeringau rhizome on the selected microorganisme including two bacterial trains Staphylococcus aureus and Escherichia coli. The rhizomes of jeringau were extracted with maceration method using ethanol 70 % and fractionation process using solvent v/v (ethanol, n-hexane and chloroform). The antibacterial activity used the cup plate method with the concentration 20%, 40%, 60%, 80%, and 100%, positive control using ampicillin and negative control using aquades. The test were continved by determining the minimum inhibitor concentration (MIC). Research was obtained zone diameters of 0 mm (negative control), 35.37 mm (positive control), 23.33 mm (concentration 20%), 25.52 mm (40%), 27.11 mm (60%), 30.09 mm (80%), and 33.16 mm (100%) against Staphylococcus aureus. Inhibitory zone diameters 0 mm negative control, 36.68 mm positive control, 22.53 mm (concentration 20%), 25.85 mm (40%), 28.08 mm (60%), 29.73 mm (80%), and 32.21 mm (100%) against Escherichia coli. The minimum inhibitory concentration (MIC) value in both bacterial were 2%. The results showed that all fraction concentrations could inhibit the growth of Staphylococcus aureus and Escherichia coli. Keywords : Rhizome Jeringau, Staphylococcus aureus, Escherichia coli.
PENGOLAHAN LIMBAH CAIR TAHU MENJADI Nata de Soya DI KABUPATEN BANJAR, KALIMANATAN SELATAN Pratika Viogenta; Amalia Khairunnisa; Samsul Hadi
Jurnal Bakti untuk Negeri Vol 2 No 1 (2022): JBN
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.902 KB)

Abstract

One of the home industries located in Martapura, Banjar Regency, is a tofu factory. The raw material for making tofu is soybean seeds which are processed so that the main product is tofu. In addition to producing tofu, the factory also produces by-products in the form of liquid waste and solid waste. So far, liquid waste is not used, but solid waste is used as animal feed. For this reason, in the application of science and technology, skills are given in the form of training to utilize liquid waste from the tofu industry into a new product, namely Nata de Soya. Nata de Soya is a highly nutritious food product, especially in the content of carbohydrates, protein and crude fiber. The methods used in this training are lectures and demonstrations. The purpose of this program is to add insight and skills to tofu industry players and the community, especially around the factory in processing liquid waste into Nata de Soya products. The results of this study increased public knowledge about the consequences of improper waste disposal and processing as well as increased knowledge of fermentation technology products and even succeeded in utilizing the tofu industrial liquid waste into processed fermented food in the form of Nata de Soya.
Bioautography and FTIR Analysis of Ethanol Fraction Morel Berry Root (Physalis angulata L.) Against Staphylococcus epidermidis and Pseudomonas aeruginosa Pratika Viogenta; Latifah Megasari; Laila Susanti
Majalah Obat Tradisional Vol 27, No 3 (2022)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.78171

Abstract

Morel berry root contains flavonoids, tannins, and alkaloids that have an antibacterial characteristics. This study aims to determine the antibacterial activity of the morel berry root ethanol fraction against Staphylococcus epidermidis and Pseudomonas aeruginosa and the most effective active compounds to inhibit such activity. Extractions of morel berry root used the maceration method with ethanol 70% of and then continued by fractionation using ethanol, chloroform, and n-hexane. Test of the compound fraction was performed using Thin Layer Chromatography (TLC) and characterization with FTIR Spectrophotometer. The results of the antibacterial activity with inhibitory zone diameter were at a 100% concentration of 18.69 mm in S. epidermidis while P. aeruginosa of 20.00 mm. Bioautographic results for ethanol fraction indicate an inhibited zone with an Rf value of 0.72 in the S. epidermidis and P. aeruginosa which is thought to be a flavonoid compound. Characterization with FTIR spectrophotometer functional groups O-H phenol, C = C aromatic, C-H aromatic, C-O alcohol, and C-H aliphatic in ethanol fraction with Rf 0.72.
Antibacterial Activity and Bioautography of the Chloroform Fraction of Morel Berry (Physalis angulata L.) Root Against Staphylococcus epidermidis and Pseudomonas aeruginosa Pratika Viogenta; Laila Susanti; Latifah Megasari
Journal of Multidisciplinary Applied Natural Science Vol. 3 No. 1 (2023): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.148

Abstract

The urgency of finding novel sources of active compounds to overcome infectious diseases is encouraged. Morel berry (Physalis angulata L.) is a traditional herbal plant that can be used as an antimicrobial because of its unique chemical content. This study aims to find compounds that have antibacterial activity from the chloroform fraction of morel berry roots. This study used the Kirby-Bauer method with concentrations of 20%, 40%, 60%, 80%, and 100% against Staphylococcus epidermidis and Pseudomonas aeruginosa bacteria. Determination of antibacterial compounds and their functional groups was performed using thin-layer chromatography (TLC) technique and reagent spray test, TLC-Bioautography and Fourier transforms infrared (FTIR) studies. The chloroform fraction of morel berry showed a zone of inhibition with the highest diameter of 11.50 and 12.00 mm against S. epidermidis and P. aeruginosa, respectively. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, and saponins in the chloroform fraction. The relative retention stain of 0.54 on the TLC plate inhibited the growth of the two tested bacteria and indicated the presence of functional groups O-H phenol, C-H aliphatic, C=O ester, C=C aromatic, C-OH alcohol, and C-H aromatic. This study found that tannins in the roots of morel berries could be used as a natural antibacterial agent to treat S. epidermidis and P. aeruginosa.
Pelatihan Penjernihan dan Pemanfaatan Minyak Jelantah Menjadi Lilin Aroma Terapi di Guntung Paikat, Banjarbaru Selatan, Kalimantan Selatan Pratika Viogenta; Sutomo Sutomo; Normaidah Normaidah
Jurnal Pengabdian ILUNG (Inovasi Lahan Basah Unggul) Vol 2, No 3 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/ilung.v2i3.6462

Abstract

Pemahaman akan pengolahan limbah di masyarakat dilakukan untuk meminimalisir pencemaran lingkungan. Salah satu limbah rumah tangga di daerah Guntung Paikat, Banjarbaru Selatan, Banjarbaru yakni adalah limbah minyak jelantah, dikarenakan banyaknya warga yang berprofesi sebagai penjual kuliner baik katering, ayam geprek dan yang lainnya, ditambah lagi minyak jelantah yang dihasilkan dari rumah tangga. Berdasarkan permasalahan tersebut, tim pengabdian kepada masyarakat (PkM) Program Studi Farmasi Universitas Lambung Mangkurat berinisiatif untuk melaksanakan suatu pelatihan pemanfaatan limbah minyak jelantah menjadi lilin aroma terapi. Tim bekerja sama dengan Kelompok PKK. Kegiatan ini dilaksanakan pada tanggal 13 Agustus 2022 dengan peserta yang hadir sebanyak 18 orang. Kegiatan tersebut terdiri dari: penyuluhan dampak dan bahaya penggunaan minyak goreng berulang-ulang, pelatihan penjernihan minyak jelantah dengan menggunakan kombinasi arang aktif kelapa sawit dan bleaching earth, demonstrasi pembuatan lilin aroma terapi berbahan dasar minyak jelantah serta evaluasi peragaan pembuatan lilin aroma terapi berbasis minyak jelantah dari para peserta pelatihan. Luaran yang dihasilkan berupa teknik pengelolaan limbah minyak jelantah, dan produk lilin aroma terapi. Secara keseluruhan kegiatan pelatihan pembuatan lilin aroma terapi berbasis minyak jelantah dan sosialisasi pentingnya mengurangi penggunaan minyak goreng berulang-ulang berjalan dengan sukses.