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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Indonesian Journal of Pharmaceutical Science and Technology Jurnal Farmasi Klinik Indonesia Acta Pharmaceutica Indonesia Indonesian Journal of Chemistry JSKK (Jurnal Sains Keolahragaan dan Kesehatan) Marine Research in Indonesia PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JFIOnline Jurnal Ilmu Kefarmasian Indonesia JURNAL FARMASI GALENIKA Jurnal Farmagazine Narra J Pharmaceutical Sciences and Research (PSR) Molekul: Jurnal Ilmiah Kimia Jurnal Kefarmasian Indonesia Journal of Tropical Pharmacy and Chemistry Jurnal Farmasi Galenika

Sophi Damayanti

Institut Teknologi Bandung

Author-ID : 249183

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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StudiIn SilicoSenyawaTurunan8-Tersubtitusi-7-Methoxy-2h-Chromen-2-OneSebagaiPenghambatEnzim Telomerase Tahir, Masdiana; Damayanti, Sophi; Hadi, Daryono; Tjahyono, Tjahyono
Jurnal Farmasi Indonesia Vol 10, No 2 (2018)
Publisher : Jurnal Farmasi Indonesia

Adjustment of telomere length by telomerase is considered as a biological marker which determine the proliferation of cancer cells. The telomerase activity in cancer cells is potential to be targeted for cancer therapies. The aim of this study was QSAR studies to determine the descriptors that affect the anticancer activity of 8-substituted-7-methoxy-2H-chromen-2-one derivatives. The descriptors were calculated by using software MOEÂ® 2009.10. to design new derivative compounds that have anticancer potency, to predict pharmacokinetic properties by using preADMET, and to predict tocixity by ADMET predictorTM and interaction predict to telomerase by using Autodock 4.2.6Â The results showed QSAR equation was log IC50 = -1.358 (Â± 1.149) - 2.957 (Â± 1.019) Ã— AM1_LUMO + 0.041 (Â± 0.011) Ã— E_vdw + 2.367 (Â± 0.748) Ã— glob + 0.487 (Â± 0.100) Ã— log S. Fourteen of the new derivative compounds were designed based on QSAR equation revealed higher activity prediction than the lead compound and performed good absorption in the intestine. Compound (33) is predicted to have low permeability in Caco-2 cells and weakly bound to plasma proteins. Compound (50) predicted weakly bound to plasma proteins as well. Toxicity prediction showed that 9 derivatives compounds were predicted to have lower nearly equal toxicity to the toxicity of the lead compound. The study of the interaction of compounds with the receptor showed that derivatives (2), (4), (40) and (41) have the highest affinity to the receptor TERT with the binding free energy is âˆ’9.60 kcal / mol, âˆ’9.39 kcal / mol, âˆ’9.20 kcal / mol and -9.08 kcal / mol. Based on QSAR study, pharmacokinetic profile, toxicity, and the study of the interaction, four compounds have the potential to be develop as anticancer with telomerase inhibition.

VERIFIKASI METODE DAN PENENTUAN KADAR LAKTOSA DALAM SAMPEL SUSU YANG BERPERISA MENGGUNAKAN KROMATOGRAPI CAIR KINERJA TINGGI Damayanti, Sophi
JSKK (Jurnal Sains Keolahragaan dan Kesehatan) Vol 4, No 1 (2019)
Publisher : Institut Teknologi Bandung

Laktosa merupakan gula disakarida dari glukosa dan galaktosa dalam susu. Sebagai sumber sumber gizi, susu dipilih oleh atlet untuk dikonsumsi. Namun demikian. laktosa dapat menyebabkan laktosa intoleransi, oleh karena itu kandungannya perlu ditentukan. Tujuan dari penelitian ini adalah untuk verifikasi metode dan penentuan kadar laktosa dalam susu yang berperisa dengan menggunakan kromatografi cair kinerja tinggi (KCKT). Sampel susu diencerkan dengan asetonitril(1:1) dan dilarutkan hingga 100mL dengan air. Susu kemudian difiltrasi dengan 0.45µm filter membrane. laktosa kemudian dianalisis menggunakan KCKT detektor indeks bias, kolom fase terikat NH2, 10µm (250µm × ID 4.6mm), dan suhu kolom pada 25oC, fase gerak asetonitril dan air (65:35) dengan laju alir adalah 1,0mL/min. Verifikasi metode menunjukkan linearitas dengan koefisien korelasi, r2 = 0.9997 dan persamaan garis y=106434,5500x-77704,1667. Koefisien fungsi regresi yang dihitung (Vx0) untuk laktosa yaitu 0.376. Pengujian presisi menghasilkan koefisien variansi untuk inter dan intraday adalah 0,3844 dan 1,6758. Persentase perolehan kembali untuk laktosa dengan metode standar adisi berada dalam rentang 99,018-101.408 %. Sampel 1 mengandung laktosa 1,277±0,283 dan sampel 2 mengandung 1,094±0,013 %. Sebagai kesimpulan, kandungan laktosa dalam susu berperisa dapat ditentukan dengan menggunakan kromatografi cair kinerja tinggi dengan detektor indek bias.

Analytical Method Development of Content and Dissolution Assay of Ursodeoxycholic Acid Capsule Wanti Megawati; Sophi Damayanti
Indonesian Journal of Pharmaceutical Science and Technology Vol 8, No 3 (2021)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v8i3.31420

Ursodeoxycholic acid is an active compound to treat cholesterol gallstones which is included in Biopharmaceutical Classification System class II. Ursodeoxycholic acid capsule formulations have been developed by the pharmaceutical industries. Due to requirement, active ingredient testing and dissolution tests need to be routinely carried out to control quality of the drug. Therefore, aim of this study was to develop a simultaneously analytical method for assay and dissolution test. Optimum condition of analysis were using HPLC-RID, C18 column (5 µm; 3.9 x 300 mm), flow rate 1 mL/min, 50 µL volume injection, acetonitrile:buffer phosphate pH 3.0 (55:45 v/v) as mobile phase, column, detector, and sample temperature were 40 oC. This method provides good linearity in a range of 50-130 % concentration for assay and dissolution test with correlation coefficient values of 1 and 0.9995, respectively. LOD and LOQ for assay were in value of 4.892 µg/mL and 14.824 µg/mL whereas for dissolution tests in a value of 6,501 µg/mL and 19,701 µg/mL. The average percent recovery for assay and dissolution test were in a range of 100.018±0.888% and 98.936±2.124 %. Repeatability and intermediate precision of assay were obtained by determining RSD values of 0.466% and 0.279-0.483 %, while the dissolution test were 1.137% and 0.032-0.289 %. The validation show this method are linear, selective, accurate, and precise.Keywords: Assay, dissolution test, Ursodeoxycholic acid, validation

Antiviral Activity and Toxicity Prediction of Compounds Contained in Figs (Ficus carica L.) by In Silico Method Sophi Damayanti; Khonsa Khonsa; Tasia Amelia
Indonesian Journal of Pharmaceutical Science and Technology Vol 8, No 1 (2021)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v8i1.29868

Viral infection is a global health problem that can cause endemic to pandemic. Compounds delivered from plants has been developed as an alternative antiviral agent. One of the plants that can be used as antiviral therapy is Ficus carica L. (figs). The aim of this research is to predict the inhibitory activity and toxicity of compounds contained in figs as an antiviral for HIV-1 using in silico method. Compounds were docked to the HIV-1 Reverse Transcriptase protein (PDB ID: 3LAL). Threedimentional structures were modeled using GaussView and optimized using Gaussian 09W. Optimized compounds were docked to the target protein using AutoDock Tools and the interaction to protein binding side were analyzed in comparison with the standard compounds. The standard compound used for the analysis nevirapine, efavirenz, and doravirine. The compound toxicity was analyzed using ECOSAR and Toxtree. Based on the results, the compounds that has similar interaction to the standard compounds were campesterol which has 4 similar hydrophobic interactions. Based on the classification of Cramer Rules for toxicity test, campesterol are classified in class 3 (high toxicity) and according to the Benigni/Bossa Rulebase classification, campesterol are negative for genotoxic and nongenotoxic carcinogenicity.Keywords: Antivirus, HIV, figs, molecular docking, toxicity

Lactobacillus plantarum as Biopreservative Agent in Paneer for Dietary Food of Diabetic and Coronary Heart Disease Patients Marlia Singgih; Kanaka L. Ahsogan; Irma M. Puspitasari; Sophi Damayanti
Indonesian Journal of Clinical Pharmacy Vol 6, No 1 (2017)
Publisher : Universitas Padjadjaran

Paneer is a food product that is obtained by heating milk followed by acid coagulation. It is known as a diet food that is recommended for diabetic and coronary heart disease patients. A relatively shorter shelf life of paneer is considered to be a major hurdle in its production. The present preliminary study was conducted to determine antibacterial activity of Lactobacillus plantarum against Eschericia coli and Bacillus cereus, to develop it as biopreservative agent in paneer before using it for dietary food of diabetic and coronary heart disease patients in next clinical study. The antibacterial activities were tested through Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) using microdilution well method. Biopreservative activity in paneer was tested using total plate count method based on time and temperature variations. Result showed that Lactobacillus plantarum inhibited Bacillus cereus and Eschericia coli with MIC of 3125 and 1562.5 µg/mL whereas MBC was in a value of >6250 and >3125 µg/mL, respectively. As a biopreservative agent, addition of Lactobacillus plantarum to paneer showed no bacterial growth until 7 days in room temperature and 9th day in cold temperature. It is concluded that Lactobacillus plantarum could be used as a natural biopreservative agent for extending the shelf life of paneer. The paneer with addition of Lactobacillus plantarum as biopreservative will then be consumed by diabetic patient and coronary heart patients in next clinical study.Keywords: Biopreservative, Lactobacillus plantarum, MBC, MIC, paneer Lactobacillus plantarum sebagai Biopreservatif pada Paneer untuk Makanan Diet Pasien Diabetes dan Jantung KoronerAbstrak Paneer adalah produk makanan yang diperoleh dengan pemanasan susu diikuti dengan koagulasi asam. Paneer dikenal sebagai makanan diet yang direkomendasikan untuk pasien dengan penyakit diabetes dan penyakit jantung koroner. Paneer memiliki ketahanan yang relatif lebih pendek sehingga dianggap menjadi masalah utama dalam produksi. Penelitian pendahuluan ini dilakukan untuk mengetahui aktivitas antibakteri Lactobacillus plantarum terhadap Eschericia coli dan Bacillus cereus, untuk mengembangkannya sebagai biopreservatif sebelum digunakan sebagai makanan diet pasien dengan penyakit diabetes dan penyakit jantung koroner pada uji klinik. Pengujian antibakteri diuji melalui Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC) menggunakan mikrodilusi metode susia. Selanjutnya, pengembangan sebagai biopreservatif pada paneer diuji menggunakan metode angka lempeng total berdasarkan waktu dan variasi suhu. Hasil penelitian menunjukkan bahwa Lactobacillus plantarum menghambat Bacillus cereus dan Eschericia coli dengan MIC 3125 dan 1562,5 µg/mL sedangkan nilai MBC masing-masing adalah 6250 dan 3125 µg/mL. Sebagai biopreservatif, penambahan Lactobacillus plantarum untuk paneer tidak menunjukkan pertumbuhan bakteri sampai 7 hari pada suhu kamar dan hari ke-9 di dalam lemari pendingin. Kesimpulannya, Lactobacillus plantarum dapat digunakan sebagai agen biopreservatif alami untuk memperpanjang masa simpan paneer yang akan dikonsumsi untuk pasien dengan penyakit diabetes dan penyakit jantung koroner pada tahap uji klinik.Kata kunci: Biopreservatif, Lactobacillus plantarum, MBC, MIC, paneer

THE CONTENT OF FATTY ACIDS IN INDONESIA’S FISH OIL Indra T Maulana; Sukraso Sukraso; Sophi Damayanti
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Study of fatty acid content was conducted in six Indonesian fish oils taken from Surabaya, Sorong, Garut, Banyuwangi, and fish oil standard. The acid and peroxide values were a main parameters which affecting the quality of the fish oil. These parameters were set using the method stated on the SNI No. 01-3555-1998. The oil containing omega-3 such as EPA and DHA is beneficial for health. The study of fatty acid content in six fish oils were analyzed by GCMS Shimadzu QP 2010 ULTRA with FID Detector. RTX-5 were used as a column (diphenyl dimethyl polysiloxane as a solid buffer, size length 30 m, diameter 0,25 mm, and He as a gas mobile phase). The results showed that acid values for oil 1, 2, 3, 4, 5, and 6 were 0.55%, 0.50%, 0.48%, 0.55%, 0.48%, and 0.58%, respectively. While the peroxide values were 5.67, 4.72, 4.45,5.01, 4.85, and 5.27 meq/kg, respectively. GCMS analysis showed that fish oil 1, 2, 3, and 4 very dominant containing squalene of 29.45%, 32.34%, 21.07%, and 43.49%, respectively. While oil 6 contained EPA of 8.97% and DHA 6.56%, and that was the highest compared with other oils. However, oil 6 also contained a trans fatty acids i.e., elaidic acid of 26.8% and trans-13-docosanoic acid of 0.9%. For comparison, natural oil 5 was rich of linoleic acid (39.58%). The GCMS's analysis results proved that the oil 6 made from lemuru had a big potency to be developed for an Indonesian fish oil export comodity. Keywords: fish oil, EPA, DHA, squalena, GCMS, acid value, peroxide value

Profil Penggunaan Terapi Bekam di Kabupaten/Kota Bandung Ditinjau Dari Aspek Demografi, Riwayat Penyakit, dan Profil Hematologi Sophi Damayanti; Fitria Muharini; Bambang Gunawan
Acta Pharmaceutica Indonesia Vol. 37 No. 3 (2012)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Pengobatan tradisional bekam tercatat sebagai salah satu pengobatan tradisional yang telah digunakan sejak 400 SM. Pada kurun waktu 5 tahun terakhir, pengobatan bekam di Indonesia berkembang pesat dengan ditandai berdirinya klinik-klinik dan asosiasi pengobatan bekam. Studi pra-penelitian menunjukkan bahwa rata-rata pasien yang mengunjungi klinik bekam di Bandung mencapai jumlah 4000 orang pasien setiap bulannya. Sementara itu, penelitian-penelitian yang terkait dengan terapi bekam masih terbatas terutama di Indonesia. Tujuan penelitian ini adalah untuk pendataan profil penggunaan terapi bekam di kabupaten/kota Bandung yang diharapkan dapat menjadi suatu studi pendahuluan yang mendorong dilakukannya penelitian ilmiah lain berkaitan dengan pengembangan terapi pengobatan yang lebih terjangkau bagi masyarakat. Penelitian ini merupakan studi deskriptif yang dilakukan dengan metode penyebaran kuesioner di klinik-klinik pengobatan tradisional bekam. Penelitian dilakukan selama periode bulan Januari sampai Mei 2012. Sebagai tambahan, pada penelitian ini juga dilakukan sampling pengambilan darah vena, darah bekam basah, dan darah perifer terkait dengan profil hematologi dan kadar glukosa darah sewaktu pengguna bekam basah. Terapi bekam banyak digunakan oleh masyarakat pada rentang 20-39 tahun (70,63%) dan 30-49 tahun (17,65%). Pendapatan pengguna bekam berkisar antara Rp 500.000,00-Rp 1.500.000,00 (37,5%). Latar belakang pendidikan pengguna terapi bekam berasal dari lulusan SMA. Terapi bekam di masyarakat digunakan untuk pengobatan (62%) dan menjaga kesehatan (38%). Bekam digunakan untuk mengobati tukak (30%), sakit kepala (28%), dan kolesterol (20%). Terdapat perbedaan bermakna pada (p<0,05) antara kondisi kesehatan responden sebelum dan sesudah menjalani terapi bekam terhadap intensitas kualitas tidur, kelelahan, pegal-pegal, dan intensitas sakit. Profil hematologi dan Sediaan Apus Darah Tepi (SADT) menunjukkan perbedaan komponen leukosit dan trombosit antara darah vena dan bekam.Secara statistik, perbedaan kadar glukosa darah sewaktu sebelum dan sesudah (p<0,05) tidak menunjukkan perbedaan berarti. Terapi bekam paling banyak digunakan oleh masyarakat usia 20-39 tahun (70,63%) dari kalangan ekonomi dengan pendapatan dibawah Rp 1.500.000,- (37,5%). Sebagian besar responden menggunakan terapi bekam untuk pengobatan tukak (30%). Tidak terdapat perbedaan yang berarti antara kadar glukosa darah sewaktu sebelum dan sesudah bekam.Kata kunci: Bekam, bekam basah, pengobatan tradisional, darah, profil penggunaan Traditional cupping treatment is listed as one of the old traditional medicine that has been used since 400 BC. In the last 5years, treatment is rapidly growing in Indonesia by the establishment of clinics and associations of cupping treatment. Preliminary-study showed that the average patient visiting a clinic in Bandung reach the 4000 number of patients each month. However, the study of cupping therapy especially in Indonesia is still limited. The aim of this study was to collect data profile of cupping therapy in Bandung region. Moreover it is expected to be a preliminary study to encourage other scientific research related to the development of more affordable therapeutic treatments for the community. This descriptive study was done by spreading questionnaire for cupping patient at tradional medicine clinics around Bandung from January-May 2012. In addition, sampling of venous blood collection, blood cupping, and peripheral blood associated with hematological profile and blood glucose level of wet cupping users were conducted. Cupping therapy was widely used by the community in the age range of 20-39 years (70.63%) and 30-49 years (17.65%). Cupping user revenues was in a range of Rp 500,000.00 -1,500,000.00 (37.5%). Educational background of cupping therapy users came from high school graduates. In the community, cupping therapy was used for treatment (62%) and health maintaining (38%). Cupping was used to treat ulcers (30%), headache (28%), and cholesterol (20%). There were significant differences (p <0.05) between the health condition before and after cupping therapy on parameters of quality of sleep, fatigue, aches, and the frequency of experiencing pain. Hematology profile showed that, blood glucose levels before and after (p <0.05) showed no significant differences. Cupping therapy in community was used by young adult in age 20-29 years old (70.63%) with income below Rp 1,500,000.00 (37.5%). Most of respondant was used cupping therapy for the treatment of ulcers (30%). Blood glucose levels before and after (p<0.05) showed no significant difference.Keywords: Cupping, wet cupping, traditional medicine, blood, profile of use.

Determination of Sugar Content in Fruit Juices Using High Performance Liquid Chromatography Sophi Damayanti; Benny Permana; Choong Chie Weng
Acta Pharmaceutica Indonesia Vol. 37 No. 4 (2012)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Sugar is a sweet, water-soluble, crystallizable material which is obtained commercially from sugarcane or sugar beet. As an important source of dietary carbohydrate, a sweetener, preservative in foods and a cause factor for Diabetic disease, analysis of sugar content is needed. The objective of this study was to verify the suitability and determination of sugar content in fruit juices using HPLC. The fresh fruits except strawberries were peeled, cut and blended using homogenizer. After filtration, 12.5mL of each fresh juices and packed juices were diluted with acetonitrile and distilled water (50:50). The diluted fresh juices and packed juices were loaded onto C18 Sep-Pak cartridge. Fructose, glucose, and sucrose of each fresh juices and packed juices were analyzed in HPLC with Refractive Index detector, NH2 polar bonded phase column, 10Î¼m (250mm Ã—4.6mm I.D.), temperature of 43.5°C, mobile phase of acetonitrile and 10mM sodium phosphate (monobasic) (78:22) and flow rate of 1.0mL/min. The method validation showed good linearity of equations for fructose, glucose and sucrose were y = 3833208.4806x "“ 94721.0361, y = 3782886.4708x "“ 101683.4708, y = 3770593.9638x "“ 82870.9083 with regression coefficients, r2 of 0.9995, 0.9997 and 0.9996 respectively. The calculated regression function coefficients (Vx0) for fructose, glucose and sucrose were 0.0152, 0.0112 and 0.0132% respectively. The percentage recovery of fructose, glucose, and sucrose was found to be in a range of each 86.681 "“ 89.888, 86.898 "“ 90.029, and 94.541 "“ 97.885%. Sugar contents in fruit juices can be determined using verified HPLC method with Refractive Index Detektor. Fructose, glucose and sucrose of fresh juices contained 0.469 "“ 1.431, 0.454 "“ 1.286, 0.544 "“ 1.861%, whereas that of packed juices contained 0.309 "“ 1.587, 0.261 "“ 0.762,0.063 "“ 0.898%, respectively.Keywords: Fructose, glucose, sucrose, fruit juice, HPLC.Gula merupakan zat yang berasa manis, larut dalam air, berbentuk kristal yang dapat diperoleh dari berbagai sumber antara lain bit gula dan tebu. Sebagai sumber karbohidrat, pemanis dan pengawet dari makanan, dan penyebab diabetes, maka kandungan gula perlu ditentukan. Tujuan dari penelitian ini adalah untuk verifikasi kesesuaian penentuan kadar gula dalam jus buah menggunakan Kromatografi Cair Kinerja Tinggi (KCKT). Buah segar kecuali stroberi dikupas kemudian dipotong dan dicampur dengan menggunakan homogenizer. Setelah disaring, 12.5mL jus segar dan 12.5mL jus kemasan diencerkan dengan asetonitril dan air suling (50:50). Jus segar dan jus kemasan kemudian dilewatkan melalui tabung C18 Sep-Pak. Fruktosa, glukosa dan sukrosa kemudian dianalisis menggunakan KCKT detektor Indeks bias, kolom NH2, 10Î¼m (250Î¼m Ã— ID 4.6mm) dan suhu kolom 43.5ºC, fase gerak asetonitril dan larutan natrium fosfat 10mM (78:22) dan laju aliran adalah 1.0mL/min. Metode validasi menunjukkan linearitas persamaan yang baik untuk fruktosa, glukosa dan sukrosa yaitu y = 3833208,4806x "“ 94721,0361, y = 3782886,4708x "“ 101683,4708, y = 3770593,9638x "“ 82870,9083 dengan koefisien korelasi, r2 adalah 0,9995, 0,9997 and 0,9996 masing-masing. Koefisien fungsi regresi yang dihitung (Vx0) untuk fruktosa, glukosa dan sukrosa adalah 0,0152, 0,0112 dan 0,0132 %. Persentase perolehan kembali untuk fruktose, glukosa dan sukrosa berada dalam rentang masing-masing 86,681 "“ 89,888, 86,898 "“ 90,029, dan 94,541 "“ 97,885%. Kandungan fruktosa, glukosa dan sukrosa dapatditentukan dengan menggunakan kromatografi cair kinerja tinggi dengan detektor indek bias. Fruktosa, glukosa dan sukrosa jus segar mengandung 0,469 "“ 1,431, 0,454 "“ 1,286, 0,544 "“ 1,861% sementara pada jus kemasan mengandung masing-masing 0,309 "“ 1,587, 0,261 "“ 0,762, 0,063 "“ 0,898%.Kata kunci: Fruktosa, glukosa, sukrosa, jus buah, HPLC.

FORMULASI DAN UJI POTENSI ANTIOKSIDAN NANOSTRUCTURED LIPID CARRIER (NLC) RETINIL PALMITAT Framesti Frisma Sriarumtias; Sasanti Tarini Darijanto; Sophi Damayanti
Acta Pharmaceutica Indonesia Vol. 42 No. 1 (2017)
Publisher : School of Pharmacy Institut Teknologi Bandung

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ABSTRAK Retinil palmitat (RP) merupakan golongan retinioid, yaitu ester vitamin A (Retinol) yang memiliki gugus palmitat pada ujung rantainya. Retinil palmitat banyak digunakan sebagai antioksidan dan anti kerut dalam industri kosmetika. Hal yang perlu diperhatikan dalam formulasi RP yaitu menjaga stabilitas dari panas, cahaya dan oksigen, sehingga perlu dicari sistem yang mampu melindungi RP dari degradasi. Salah satunya dibuat dalam sistem Nanostructured lipid carriers (NLC), sebagaimana diketahui bahwa NLC merupakan sistem penghantaran obat yang bisa meminimalisir degradasi. Tujuan penelitian ini adalah untuk mengembangkan sistem penghantaran NLC untuk meningkatkan stabilitas serta potensi antioksidan dan meningkatkan difusi RP melalui kulit. Bahan-bahan yang digunakan untuk pembuatan NLC dengan metode teknik mikroemulsi yaitu 4% PEG-8 beeswax dan 4% Isopropil miristat sebagai lipid padat dan lipid cair, 13% Polisorbat 80 sebagai surfaktan, dan 10% Sukrosa stearat sebagai kosurfaktan. Karakterisasi yang dilakukan yaitu pengukuran ukuran partikel dan polidisperistas indeks, efisiensi penjerapan, uji difusi dengan Franz diffusion cell, karakterisasi morfologi dengan Transmission electron microscopy (TEM) dan uji aktivitas antioksidan dengan DPPH. NLC-RP memiliki ukuran partikel 65,63±1,09 nm, indeks polidispersitas 0,32±0,07, efisiensi penjerapan 94,55±0,76%, hasil uji difusi tertinggi yaitu pada NLC-RP 52,58±4,37%, diikuti krim NLC-RP 36,36±1,46%, krim RP 18,70±2,13% dan emulsi RP 18,22±1,50%. Uji stabilitas fisika dan kimia NLC-RP disimpan selama 60 hari pada suhu 25°C RH 65% dan 40°C RH 75% menunjukan bahwa tidak ada perubahan pada kondisi tersebut. Dari penelitian ini didapat kesimpulan bahwa NLC-RP mampu menjaga stabilitas retinil palmitat. Sedangkan NLC-RP yang dimasukan kedalam krim mengalami penurunan kadar sebanyak 58,15% pada suhu ruang dan 70,05% pada suhu 40°C serta penurunan potensi antioksidan akibat keberadaan basis krim.Kata kunci: antioksidan, DPPH, nanostructured lipid carrier, retinil palmitatRETINYL PALMITATE NANOSTRUCTURED LIPID CARRIER (NLC) FORMULATION AND ANTIOXIDANT POTENTIAL TEST ABSTRACT Retinyl palmitate (RP), member of retinoid family is an ester of retinol with palmitate functional group at the end of the chain. RP is commonly used as antioxidants and anti-wrinkle component in cosmetic industry. RP instability requires a formulation system which makes it stable against heat, light and oxygen, such as Nanostructured Lipid Carriers (NLC). NLC is known as notable drug delivery system to minimize degradation. The aim of this research is to develop NLC delivery system to improve stability and antioxidant activity and increase RP diffusion through the skin. Materials used in NLC formulation were obtained using microemulsion technique are 4% PEG-8 beeswax and 4% isopropyl myristate as lipids in solid and liquid form, respectively; 13% polysorbate 80 as surfactant and 10% sucrose stearate as cosurfactant. Characterization NLC were evaluated using particle size measurement, polydispersity index, entrapment efficiency, in vitro diffusion testing using Franz diffusion cell, morphological characterization using Transmission Electron Microscopy (TEM) and antioxidant activity using DPPH. Particle size of NLC-RP was 65,63±1,09 nm, polydispersity index of 0,32±0,07, entrapment efficiency of 94,55±0,76%. Penetration result showed liquid NLC-RP difusion the highest 52,58±4,37%, followed by NLC-RP in cream 36,36±1,46%, RP in cream 18,70±2,13% and RP in emulsion 18,22±1,50%. Physical and chemical stability testing NLC-RP were stored for 60 days at 25°C RH 65% and 40°C RH 75%, the results shown there are no changed in these condition. Research results showed NLC-RP is able to maintain stability of RP, meanwhile NLC RP in cream formulation shows 58.15% amount decrease in room temperature and 70.05% amount decrease at 400C, and antioxidant activity decrease due to cream formulation.Keywords: antioxidant, DPPH, nanostructured lipid carrier, retinyl palmitate

Pengaruh Penyiapan Sampel pada Pengembangan Metode Analisis Laktosa dalam Susu Formula Menggunakan Kromatografi Cair Kinerja Tinggi Mardiana Mardiana; Sophi Damayanti; Slamet Ibrahim
Acta Pharmaceutica Indonesia Vol. 39 No. 1 & 2 (2014)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Penelitian ini menggunakan tiga jenis prosedur preparasi sampel untuk penetapan kadar laktosa dari susu formula bayi menggunakan KCKT. Kondisi optimum KCKT adalah sebagai berikut: detektor indeks refraksi, kolom NH2, fase gerak ACNH2O (65:35) dengan laju alir 1 mL/menit. Metode ini memberikan linieritas dengan r 0,9998; koefisien variansi regresi 1,25%; batas deteksi 0,12 mg/mL; dan batas kuantitasi 0,35 mg/mL. Prosedur preparasi III memberikan keseksamaan dan keakuratan yang lebih baik dibandingkan prosedur I dan II, mengindikasikan bahwa prosedur preparasi sampel adalah tahap krusial pada penentuan kadar laktosa dalam susu formula bayi.Kata kunci: laktosa, susu formula bayi, preparasi sampel, kromatografi cair kinerja tinggi, detektor indeks refraksiAbstractThis research performed three different sample preparation procedure before the determination of lactose content from infant milk formula using HPLC technique under following optimum conditions: refractive index detector, NH2 column, mobile phase ACN-H2O (65:35) with the flow rate 1 mL/minute. The method gave calibration curve with r = 0.9998; regression coefficient variance 1.25%; limit of detection 0.12 mg/mL; and limit of quantitation 0.35 mg/mL. Procedure preparation III gave precision and recovery better than procedure I and II, indicating that the sample preparation is crucial step in the determination of lactose in infant milk formula.Keywords: lactose, infant milk formula, sample preparation, high performance liquid chromatography, refractive index detector

Co-Authors Adi Suwandi Adi Suwandi Ahsogan, Kanaka L. Aluicia Anita Artarini Anjar Hermadi Saputro Bambang Gunawan Choong Chie Weng Daryono H Tjahjono Dian Ayu Eka Pitaloka Dina Pratiwi S.Si, M.Si Dwiyanti, Jessica Eko Prayitno Elin Yulinah Sukandar Engrid Juni Astuti Farel Fahrizal Fathurohman, Mochamad Fitria Muharini Framesti Frisma Sriarumtias Galih, Wening Gunawan, Bambang Hadi, Daryono Hariyanti Hariyanti HENI RACHMAWATI Indra Maulana` Indra Maulana Indra Topik Maulana Indra Wibowo Indra Wibowo Irma M. Puspitasari Irma M. Puspitasari, Irma M. Iskandar, Fery Julianti, Elin Julianti, Elin Kanaka L. Ahsogan Kartasasmita, Rahmana Emran Khonsa Khonsa Kurnia Firman Kusnandar Anggadiredja LIA AMALIA Mardiana Mardiana Mardiana, Mardiana Marlia Singgih Marlia Singgih Masdiana Tahir Muhammad Ali Zulfikar Muhammad Insanu Muharini, Fitria Nadiyah Athifah Salim Martak Nur Mita Nurwulan, Sita Permana, Benny Permana, Benny Permatasari, Fitri A. Pinanga, Yangie Dwi Marga Rahmana Emran Kartasasmita Rahmana Emran Kartasasmita Rasida, Rima Reyaan, Irianti Bahana Maulida Rika Hartati Saeful Amin Saeful Amin, Saeful Samuel Gunadi Tanusondjaja Sasanti Tarini Darijono Slamet Ibrahim Slamet Ibrahim - Sriarumtias, Framesti Frisma Sukrasno Sukrasno Sukraso Sukraso Tasia Amelia Tjahyono Tjahyono Triantri Kartika Putri Vita Yuanita Wanti Megawati Weng, Choong Chie Wibowo, Indra Wijayanti, Ni PAD. Yuanita, Vita

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