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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Plumula : Berkala Ilmiah Agroteknologi AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Jurnal Agrotek Tropika Asian Journal of Agriculture Agro Bali: Agricultural Journal Nandur
Ida Ayu Putri Darmawati
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Meristem Culture of Strawberry Plants (Fragaria x Ananassa Duch.) Sachinoka Variety on Various Types of Basic Media In Vitro Fitriani, Yuyun; Yuswanti, Hestin; Dwiyani, Rindang; Darmawati, Ida Ayu Putri
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p08

Abstract

The large number of in vitro media circulating in the market is a challenge to find the most effective media for sachinoka strawberry meristem culture, because the need for in vitro media depends on genetic intelligence. The purpose of this study was to find the best media from the various tested media to reproduce the Sachinoka variety strawberry seedlings in a relatively short time using the meristem culture method. This study used a completely randomized design (CRD) with 6 media treatments and repeated 7 times, namely: M1 (WPM without BA), M2 (WPM + 4 ppm BA), M3 (NP without BA), M3 (NP + 4 ppm BA), M5 (BMM without BA), M6 (BMM + 4 ppm BA). The variables observed were the time of emergence of shoots, the number of shoots and the number of leaves. The results of this study indicate that there is no significant difference for the emergence of shoots variable. Treatment M1 produced the highest number of shoots (12.29) but not significantly different from M5 (10.14), both were significantly different from both treatments. The highest number of leaves is in M1 (27.14). It can be concluded that the best medium for the growth of sachinoka strawberry meristem culture is WPM media with the addition of 4 ppm BA, while the second-best medium is BMM media with the addition of 4 ppm BA.
Pengaruh Waktu Pemberian dan Konsentrasi Hormon NAA (Napthalene Acetic Acid) terhadap Pertumbuhan dan Hasil Stroberi (Fragaria ananassa Duch) untuk Mendukung Pengembangan Agrowisata di Desa Pancasari, Bali Khusni, Mazroatul; Rai, I Nyoman; Darmawati, Ida Ayu Putri
Plumula : Berkala Ilmiah Agroteknologi Vol. 11 No. 2 (2023): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v11i2.201

Abstract

Kendala terbesar pada budidaya tanaman stroberi di agrowisata Desa Pancasari, Buleleng, Bali adalah kerontokan bunga dan buah. Upaya untuk mengatasi hal tersebut dapat dilakukan dengan pemberian NAA (Napthalene Acetic Acid). Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi NAA dan waktu aplikasi pada pertumbuhan dan hasil stroberi. Penelitian dilaksanakan di Desa Pancasari pada bulan November 2020-April 2021. Percobaan menggunakan Rancangan Acak Kelompok dengan dua faktor. Faktor pertama adalah konsentrasi NAA terdiri atas empat taraf, yaitu 0, 50, 100, dan 200 ppm. Faktor kedua adalah waktu aplikasi terdiri atas 3 taraf, yaitu aplikasi pada fase berbunga, fase berbuah, dan fase berbunga + berbuah. Hasil penelitian menunjukkan konsentrasi NAA 200 ppm meningkatkan hasil stroberi yaitu bobot per buah sebesar 12,52% (1,88 g) dan diameter buah sebesar 10,46% (2,35 mm). Waktu aplikasi NAA pada fase berbunga + berbuah meningkatkan jumlah buah panen per tanaman sebesar 40,55% (4,45 buah), diameter buah sebesar 8,68% (1,98 mm) dan bobot buah panen per tanaman 71,78%. Sementara waktu aplikasi NAA pada fase berbunga meningkatkan pertumbuhan pada persentase fruit set sebesar 25,13% (114,33 g), jumlah bunga per tanaman sebesar 93,81% (2,58 kuncup, jumlah buah terbentuk per tanaman 80,85% (70,25 buah), serta total padatan terlarut sebesar 16,03% (1,39 brix). Interaksi antara konsentrasi NAA dan waktu aplikasi berpengaruh nyata terhadap tinggi per tanaman dan bobot buah panen per tanaman. Kombinasi konsentrasi NAA 100 ppm dan waktu aplikasi pada fase berbunga + berbuah dapat menekan tinggi per tanaman sebesar 2,14 cm atau menekan 8,72%, tetapi meningkatkan bobot buah panen per tanaman sebesar 263,26 g atau meningkat 236,45%.
The Role of Mycorrhizae on the Growth of Banana Planlets of Cultivar Raja (Musa Paradisiaca C.V. Raja) from Bali at Post Acclimatization: Role of Mycorrhizae on the Growth of Banana Planlets Dwiyani, Rindang; Wirya, Gusti Ngurah Alit Susanta; Gunadi, I Gusti Alit; Darmawati, Ida Ayu Putri; Yuswanti, Hestin; Susrusa, Ketut Budi; AStiningsih, Ana Agung Made
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.11

Abstract

The effect of inoculation of a combination of the arbuscular mycorrhizal fungus of Glomus and Acaulospora on micropropagated banana plantlets cultivar Raja from Bali during the acclimatization phase was investigated. The experiment was conducted at the Experimental Station of the Faculty of Agriculture, Udayana University, Denpasar, Bali, Indonesia, over the period of March to June 2022. The objective of this research was to evaluate the impact of various doses of mycorrhizae on the growth of tissue culture banana plantlets that had undergone acclimatization for a period of six weeks. A total of five different doses of mycorrhiza were applied to the acclimatized plantlets, namely M0=without mycorrhiza and M5=5g mycorrhiza.kg-1, M10=10 g mycorrhiza.kg-1, M15=15 g mycorrhiza.kg-1, M20=20 g mycorrhiza.kg-1. A significant effect was discovered at M15; however, compared to M20, M20 gave better results for root length and fresh weight of the above-ground part, indicating the best dose was 20 g mycorrhiza kg-1. This study concluded that mycorrhiza has a very significant role in supporting the growth of micro propagated banana plantlets at post-acclimatization. Variables observed, such as the average number of leaves, stem diameter, above-ground plant fresh and dry weight, and root fresh and dry weight, increased with the application of AMF (Glomus and Acaulospora).
MORPHOLOGICAL CHARACTERIZATION AND PHYTOCHEMICAL ANALYSIS OF RATNA FLOWER PLANT (Gomphrena globosa) PINK PHENOTYPE IN SIBANGGEDE AND PADANG GALAK AREAS Redana, I Made Basma; Dwiyani, Rindang; Darmawati, Ida Ayu Putri
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.9681

Abstract

The Ratna flower (Gomphrena globosa L.), commonly known as globe amaranth, is widely used as an ornamental plant, natural tea ingredient, food coloring, and traditional medicine due to its various health benefits. In Bali, this flower also holds important cultural value as an offering in ceremonies and sacred dances. Despite its diverse uses, studies on the morphological and phytochemical diversity of G. globosa accessions across different growing regions remain limited. This study aimed to characterize the morphological traits and analyze the phytochemical composition of pink-flowered Ratna accessions collected from two regions, Sibanggede and Padang Galak. The research was conducted from October to December 2023. Sampling areas were determined using an exploratory method with purposive sampling, and plant samples were collected using a simple random sampling approach. The findings revealed distinct morphological variations in leaf and stem surfaces between accessions from the two locations. Phytochemical analysis showed qualitative and quantitative differences in chemical composition. The Sibanggede accession contained major compounds such as phytol, ethyl ester, and hexadecanoic acid—known for their antioxidant, anticancer, and antibacterial properties. In contrast, the Padang Galak accession was dominated by compounds including 2(4H)-Benzofuranone and thiourea, which exhibit antiarthritic and anticancer activities. These morphological and phytochemical differences are likely influenced by environmental factors affecting plant development and secondary metabolite synthesis. Overall, this study provides baseline information for the cultivation and utilization of Ratna flower in pharmaceutical, cosmetic, and ethnobotanical applications.
Effectiveness of Various Scarification Methods on Breaking Seed Dormancy in Some Types of Palm (Arecaceae) Setora, Ketut Weda; Wijana, Gede; Darmawati, Ida Ayu Putri
Agro Bali : Agricultural Journal Vol 8, No 1 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i1.1995

Abstract

The palm breeding and cultivation challenges are mainly related to the seed germination process. Various factors, including seed dormancy, often influence this process. Various scarification methods and several types of palms that can play a role in breaking dormancy need to be tried. This research aims to determine the best dormancy-breaking scarification method for various palm seeds. This research was carried out in Bakung Village, Sukasada District, Buleleng Regency, from January to March 2024, using a Nested Design comprising two factors. The first factor is that the type of palm seed consists of four levels: Putri palm, Pinang palm, Squirrel Tail palm, and Green palm. The second factor consists of six levels, namely control method (without peeling), peel scarification method, peel scarification method+warm water, peel scarification method+H2SO4, peel scarification method+KNO3, peel scarification method+GA3. The results of the research showed that the scarification method had a significant effect (p<0,05) on the percentage of sprouts and shoot length. Very significant effect (p<0,01) on the variables of germination, maximum growth potential, seed growth speed, and root length, had an not significant effect on the synchronization of seed growth. This research concludes that the scarification method affects breaking seed dormancy in several types of palm, except for the areca palm.
Optimizing Seedling Density per Planting Hole of Lettuce (Lactuca sativa L. var. longifolia) in a Deep Flow Technique Hydroponic System Febriana, I Gede; Wijana, Gede; Sukewijaya, I Made; Darmawati, Ida Ayu Putri; Pradnyawathi, Ni Luh Made
Agro Bali : Agricultural Journal Vol 8, No 3 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i3.2441

Abstract

Market demand for baby romaine lettuce (Lactuca sativa L. var. longifolia) continues to rise alongside the public’s growing preference for healthy lifestyles, yet its productivity still needs improvement. This study aims to evaluate the effect of seedling density per planting hole on the growth and yield of baby romaine lettuce using the Deep Flow Technique (DFT) hydroponic system and to determine the optimal number of seedlings. The experiment was conducted in a greenhouse in Pelaga Village, Bali, using a completely randomized design (CRD) with a single factor comprising five seedling densities (1, 2, 3, 4, and 5 seedlings per hole) and six replications, totaling 120 plants.  Growth and yield parameters were analyzed using ANOVA and LSD at a 5% significance level, with regression applied to fresh shoot and crop weight. Results showed that increasing the number of seedlings significantly affected plant height, stem diameter, chlorophyll content, fresh and dry shoot weight, and total fresh biomass. Shoot fresh weight increased linearly (Y = 97.86 + 11.71X; R² = 0.975), while crop fresh weight followed a quadratic trend (Y = 70.64 + 8.10X – 1.00X²; R² = 0.863), with the optimal yield obtained at three seedlings per hole. It is concluded that planting three seedlings per hole offers the best outcome in terms of both quality and quantity in a DFT hydroponic system.
Gibberellin and potassium sorbate synergy for parthenocarpy and sugar enhancement in Bali grapes, Indonesia KAMUHI, RINI HERMINA; DWIYANI, RINDANG; DARMAWATI, IDA AYU PUTRI
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090223

Abstract

Abstract. Kamuhi RH, Dwiyani R, Darmawati IAP. 2025. Gibberellin and potassium sorbate synergy for parthenocarpy and sugar enhancement in Bali grapes, Indonesia. Asian J Agric 9: 568-581. Bali grape (Vitis vinifera L. var. Alphonso Lavallee) is a high-value horticultural commodity; however, its fruit quality, particularly sweetness and seed content, often falls short of consumer expectations. This study aimed to evaluate the effects of gibberellin (GA3) and potassium sorbate applications on parthenocarpy induction and sugar content enhancement. The experiment employed a factorial randomized complete block design with two factors: GA3 concentrations (0, 50, 100, and 150 ppm) and potassium sorbate concentrations (0 and 0.3%), each with five replications, resulting in 40 experimental units. Results showed that both GA3 and potassium sorbate significantly affected cluster and fruit characteristics, including cluster length, pedicel length, fruit number and weight, as well as seed number and weight. The interaction between the two treatments had a significant effect on berry diameter, number of millerandage (undeveloped) berries, and sugar content. The combined application of 150 ppm GA3 and 0.3% potassium sorbate increased berry diameter to approximately 19 mm and sugar content by about 38% to 19°Brix, while reducing the number of millerandage berries to 1.60 per cluster. GA3 at 150 ppm effectively reduced seed number and seed weight, while increasing fruit number, fruit weight, cluster weight, and cluster size. Potassium sorbate also contributed to improving the physical quality of the fruit. These findings indicate a synergistic effect between GA3 and potassium sorbate. The combined application, which is both practical and cost-effective, offers a promising agronomic strategy to improve the fruit quality of Bali grape, thereby enhancing its competitiveness in both domestic and international.
Genetic diversity and relationships among native Dendrobium species from Bali, Indonesia, using SSR markers DARMAWATI, IDA AYU PUTRI; DWIYANI, RINDANG; PRADNYAWATHI, NI LUH MADE; FITRIANI, YUYUN; LISTIHANI, LISTIHANI; KAMUHI, RINI HERMINA
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090200

Abstract

Abstract. Darmawati IAP, Dwiyani R, Pradnyawathi NLM, Fitriani Y, Listihani L, Kamuhi RH. 2025. Genetic diversity and relationships among native Dendrobium species from Bali, Indonesia, using SSR markers. Asian J Agric 9: 917-924. Bali, Indonesia, has significant potential for the development of orchid cultivation, particularly through the production of hybrids that meet market preferences. Among various orchid genera, Dendrobium holds major economic and horticultural importance due to its wide adaptability, aesthetic value, and high demand in both domestic and international markets. Wild Dendrobium species represent an invaluable source of germplasm that can be used for interspecific hybridization and the development of new cultivars with desirable characteristics such as flower color, shape, and durability. However, the lack of information on the genetic diversity of native Dendrobium species in Bali limits their effective use in breeding and conservation programs. This study aimed to evaluate the genetic diversity and relationships among 24 native Dendrobium species collected from 12 forest areas across Bali using Simple Sequence Repeat (SSR) markers. The molecular analysis revealed high genetic variation, with 86.21 percent of loci showing polymorphism and moderate heterozygosity across populations. Genetic similarity coefficients among species ranged from 0.66 to 0.97. The highest genetic similarity was observed between Dendrobium sp. Wanagiri and Dendrobium sp. Sepang, while D. fimbriatum was identified as the most genetically distinct species. These findings demonstrate a broad genetic base among Bali’s native Dendrobium species, indicating their strong potential as parental resources in hybrid breeding programs. The study also provides valuable molecular data that support the conservation and sustainable utilization of Bali’s native orchid germplasm for future cultivation and breeding initiatives.
Co-Authors - Afrizal A.A.N.B. KAMANDALU AGUNG WIDYA ANTASARI DEWI ANAK AGUNG MADE ASTININGSIH ANAK AGUNG NGURAH DHARMA SEPUTRA AStiningsih, Ana Agung Made Cokorda Gede Alit Semarajaya Dewi, Ni Putu Elvina Kusuma DIMAS MANDALA PUTRA DINI WIRDASARI Febriana, I Gede GEDE WIJANA Gusti Ngurah Alit Susanta Wirya H. Yuswanti HESTIN YUSWANTI I GEDE PUTU WIRAWAN I Gusti Alit Gunadi I GUSTI ALIT GUNADI I Ketut Sardiana I Ketut Suada I MADE AGUS KRISNA I MADE RAI YASA I Made Sukewijaya I NYOMAN GEDE ASTAWA I NYOMAN RAI I PUTU DIAN PRATAMA I WAYAN SUNANJAYA I.G.A. Gunadi IDA AYU ASTARINI IDA AYU MAYUN Ida Ayu Sri Dewi Adriani IDA BAGUS KADE SUASTIKA K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KAMUHI, RINI HERMINA KETUT BUDI SUSRUSA KETUT SUADA Ketut Suada Khusni, Mazroatul Listihani, Listihani Maheswari, Mea Aprilia Manullang, Prila Kartika Mulyani, Iluh Sri N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI NENGAH DARMIATI NI NYOMAN ARI MAYADEWI NI PUTU SUTAMI NI WAYAN SURIANTARI Noviani, Made PUTRI REZKY Redana, I Made Basma Retno Kawuri Rindang Dwiyani S.A.N. ARYAWATI Sanjaya, I Putu Wahyu Setora, Ketut Weda TRISNA AGUNG PHABIOLA Y. Fitriani Yuyun Fitriani