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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Plumula : Berkala Ilmiah Agroteknologi AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Jurnal Agrotek Tropika Agro Bali: Agricultural Journal Nandur
Ida Ayu Putri Darmawati
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Pengaruh Konsentrasi AB Mix dan Frekuensi Semprot Pupuk Organik Cair (POC) terhadap Pertumbuhan Tanaman Pakcoy (Brassica rapa L.) pada Hidroponik Sistem Deep Floating Technique (DFT) I MADE AGUS KRISNA; GEDE WIJANA; IDA AYU PUTRI DARMAWATI
Agrotrop : Journal on Agriculture Science Vol 13 No 1 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i01.p04

Abstract

Effect of AB Mix Concentration and Frequency of Liquid Organic Fertilizer Spray (POC) on the Growth of Pakcoy (Brassica rapa L.) Plants in the Deep Floating Technique (DFT) Hydroponic System. Pakcoy (Brassica rapa L.) is one of the vegetables that can be cultivated using a hydroponic system. Deep Floating Technique (DFT) hydroponic system is one of the hydroponic techniques in vegetable cultivation by using pools in installations and circulation with slow flow. The nutrients used in hydroponic cultivation are generally AB mix nutrients, but the use of liquid organic fertilizer (pupuk organik cair/POC) in hydroponic vegetable cultivation needs more attention to suppress the use of inorganic fertilizers. The study aims to determine the response of pakcoy growth to the addition of POC and to obtain the concentration of AB mix and the frequency of POC spray which gives the best pakcoy plant growth. This research was carried out from January to March 2021 in a green house with a UV plastic roof in Banjar Gentong, Tegallalang District, Gianyar Regency, Bali. Geographically, it is located at 8°48'38 south latitude and 115°28'04 east longitude at an altitude of 510-550 meters above sea level. The study used a Nested Randomized Block Design where the POC spraying frequency was nested at the concentration of AB mix. The data obtained from the variables were analyzed statistically using analysis of variance (ANOVA) according to the design that was carried out. If the treatment has a significant effect, then the BNT test at the 5% level will be carried out. The addition of POC spraying frequency on pakcoy showed a significant effect, the highest yield ware shown in the addition of POC spraying frequency once every 2 days at all concentration treatment levels. The combination of giving AB mix 1,000 ppm with the POC spraying frequency every 2 days (K3F1) gave the best growth for pakcoy.
PELATIHAN AKLIMATISASI BIBIT STROBERI HASIL KULTUR MERISTEM BAGI PETANI STROBERI PANCASARI R. Dwiyani; K.B. Susrusa; I.A.P. Darmawati; G.N.A.S. Wirya; I.G.A. Gunadi; N.N.A. Mayadewi; H. Yuswanti; K.A. Yuliadhi; T.A. Phabiola; R. Kawuri; Y. Fitriani
Buletin Udayana Mengabdi Vol 22 No 3 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i03.p10

Abstract

Abstracts The community service activity entitled "Training for Acclimatization of Strawberry Seeds from Meristem Culture for Pancasari Strawberry Farmers" has been completed. This activity was motivated by the problem of seeds by strawberry farmers in Pancasari, where farmers needed healthy seeds from tissue culture, because through propagation with runners, crop yields decreased drastically due to virus attacks. The solution is the use of seeds from meristem culture. Acclimatization training by farmers is important because removing seeds from culture bottles and then growing them on media ex vitro requires skills and understanding of acclimatization theory. The purpose of this service was to provide strawberry acclimatization skills to farmers, so that farmers can acclimatize themselves to meristem cultured strawberries produced by the Tissue Culture Laboratory of the Faculty of Agriculture Udayana University. This activity was based on the results of strawberry meristem culture research in 2019 funded by the ministry of research technology and higher education Republic of Indonesia through a national competitive grant. The results of the current community service concluded several things as follows: The training participants, especially farmers, have succeeded in carrying out their own acclimatization practice; Strawberry seedlings grown in the highlands (Pancasari) had better vigor than those grown in the lowlands (Denpasar). Keywords: acclimatization, meristem culture, Pancasari, strawberry, virus.
Pengaruh Penambahan Gula dan Tepung Terigu pada Media Tanam Dasar terhadap Pertumbuhan dan Hasil Jamur Tiram (Pleurotus ostreatus) PUTRI REZKY; NI LUH MADE PRADNYAWATHI; IDA AYU PUTRI DARMAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.4, Oktober 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Effect of Sugar and Flour Addition into the Basic Planting Media on the Growth and Yield of Oyster Mushrooms (Pleurotus ostreatus) The purpose of this study was to get the best composition from the addition of sugar and wheat flour to the basic growing media for the growth and yield of oyster mushrooms. The research was conducted from May to October 2020 at the UPTD Balai Benih Induk Tanaman Pangan dan Hortikultura, Desa Luwus, Tabanan, Bali. This study used a Complete Randomized Design (CRD) of one factor, namely the mixed material of the planting media. The treatment in this study consisted of: basic planting media with added sugar and wheat flour (sawdust, bran, chalk, sugar, and wheat flour), basic planting media with added sugar (sawdust, bran, chalk, and sugar), basic planting media with the addition of wheat flour (sawdust, bran, chalk, and wheat flour) and basic planting media (sawdust, bran, and chalk). According to the results of research and analysis, it can be concluded that the addition of granulated sugar to the basic planting media of oyster mushroom caused the growth of the mycelium was longer and the size of the hood and the length of the stalk was smaller. The addition of wheat flour to the basic planting media caused the number of oyster mushroom fruit bodies to be less, but has a large hood size. The addition of granulated sugar and wheat flour to the basic growing media of oyster mushrooms caused in a faster total growth time of the mycelium, larger hood size and stalk length, but has fewer fruit bodies and a light wet weight.
The Role of Mycorrhizae on the Growth of Banana Planlets of Cultivar Raja (Musa Paradisiaca C.V. Raja) from Bali at Post Acclimatization: Role of Mycorrhizae on the Growth of Banana Planlets Dwiyani, Rindang; Wirya, Gusti Ngurah Alit Susanta; Gunadi, I Gusti Alit; Darmawati, Ida Ayu Putri; Yuswanti, Hestin; Susrusa, Ketut Budi; AStiningsih, Ana Agung Made
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.11

Abstract

The effect of inoculation of a combination of the arbuscular mycorrhizal fungus of Glomus and Acaulospora on micropropagated banana plantlets cultivar Raja from Bali during the acclimatization phase was investigated. The experiment was conducted at the Experimental Station of the Faculty of Agriculture, Udayana University, Denpasar, Bali, Indonesia, over the period of March to June 2022. The objective of this research was to evaluate the impact of various doses of mycorrhizae on the growth of tissue culture banana plantlets that had undergone acclimatization for a period of six weeks. A total of five different doses of mycorrhiza were applied to the acclimatized plantlets, namely M0=without mycorrhiza and M5=5g mycorrhiza.kg-1, M10=10 g mycorrhiza.kg-1, M15=15 g mycorrhiza.kg-1, M20=20 g mycorrhiza.kg-1. A significant effect was discovered at M15; however, compared to M20, M20 gave better results for root length and fresh weight of the above-ground part, indicating the best dose was 20 g mycorrhiza kg-1. This study concluded that mycorrhiza has a very significant role in supporting the growth of micro propagated banana plantlets at post-acclimatization. Variables observed, such as the average number of leaves, stem diameter, above-ground plant fresh and dry weight, and root fresh and dry weight, increased with the application of AMF (Glomus and Acaulospora).
Kandungan Fitokimia dan Aktivitas Antioksida dari Daun Tumbuhan Senduduk (Melastoma malabathricum L.) dengan Metode GC-MS (Gas Chromatography-Mass Spectrometry) Afrizal, Afrizal; Wirawan, I Gede Putu; Darmawati, Ida Ayu Putri
Agrotrop : Journal on Agriculture Science Vol 13 No 3 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i03.p10

Abstract

Phytochemical Content and Antioxidant Activity of the Leaves of the Senduduk Plant (Melastoma malabathricum L.) using the GC-MS (Gas Chromatography-Mass Spectrometry) Method. Senduduk (Melastoma malabathricum L) is one of the plants that can be used as traditional medicine. This study aims to determine the types of active compounds, levels of active compounds, and levels of antioxidant compounds in the leaves of the senduduk plant. This research lasted for 3 months. The method used in this study is a descriptive method with laboratory analysis techniques. Active compound analysis testing with GCMS analysis, testing and phytochemical levels, as well as determining the activity and levels of antioxidants. The results showed that the active compounds in senduduk leaf extract were mostly dominated by phenolic compounds and saponin compounds. The phenolic compound group contains active compounds 1,2,3-Benzenetriol and Vitamin e dl-.alpha.-Tocopherol with a percentage of 26.252% and the saponin group contains active compounds by fatty acids, namely hexanacanoic acid and linoleic acid with an area proportion of 14.25. %. The levels of active compounds from the phytochemical screening of the active compound group with the strongest levels were Alkaloids, Phenolics, and Saponins. The active compound group with moderate levels was only terpenoid active compounds, while the active compounds with sufficient levels contained steroids, flavonoids, and tannins which were tested by the Liebermann, Buchard, and Meyer reagent examination methods. The antioxidant activity in the extract of the leaves of this senresiden is strong enough, namely 70,607 ppm.
Pengaruh Ekstraksi Benih dengan HCl dan Jenis Wadah Penyimpanan terhadap Daya Simpan Benih Tomat (Lycopersicum esculentum Mill.) Maheswari, Mea Aprilia; Astawa, I Nyoman Gede; Darmawati, Ida Ayu Putri
Agrotrop : Journal on Agriculture Science Vol 14 No 1 (2024)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Effect of Seeds Extraction with HCl and Types of Storage Packaging on Storability of Tomato Seeds (Lycopersicum esculentum Mill.). This study aims to determine the effect of seeds extraction with HCl and types of storage packaging on storability of tomato seeds (Lycopersicum esculentum Mill.). This study used a factorial Completely Randomized Design with two factors. The first factor was HCl extraction which consists of five levels, namely HCl concentration 0% (K0), 1% (K1), 2% (K2), 3% (K3) and 4% (K4). The second factor was the type of storage packaging which consists of three levels, namely aluminium foil (JA), plastic clip (JP) and glass bottles (JB). Each treatment was repeated 3 times. The research was conducted at the Plant Breeding and Seed Technology Laboratory, Faculty of Agriculture, Udayana University from September-December 2022. The result showed the storage for 12 weeks used HCl extraction 2% in aluminium foil (K2JA) obtained the best average value to maintain the storabilitu of tomato seeds which is indicated by the value of water content (5,04%), germination (86,66%), simultaneous growth (82,22%) and vigor storability (82,22%) compared to another HCl concentration and storage packaging.
Pengaruh Varietas dan Berat Umbi terhadap Viabilitas Bibit Bawang Merah (Allium ascalonicum L.) Mulyani, Iluh Sri; Darmawati, Ida Ayu Putri; Mayun, Ida Ayu
Agrotrop : Journal on Agriculture Science Vol 14 No 2 (2024)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2024.v14.i02.p03

Abstract

The Effect of Variety and Bulb Weight on the Viability of Shallot Seedlings (Allium ascalonicum L.). The demand for shallots continues to increase so production needs to be increased, one of the efforts that can be made is to provide good quality seeds. Viability is a benchmark in determining seedling quality. Seedling viability can be influenced by variety and bulb weight. This study aims to determine the effect of variety and bulb weight on the viability of shallot seedlings (Allium ascalonicum L.). The experimental design used is a completely randomized design (CRD) factorial pattern with two factors. The first factor is variety, namely Bali Karet, Biru Lancor, and Batu Ijo. The second factor is bulb weight, which is large (12-16 g), medium (8-12 g), and small (4-8 g). The research was conducted in the Greenhouse of the Experimental Garden of the Faculty of Agriculture, Udayana University, Denpasar from February to April 2023. The results showed that the variety treatment had a significant effect on the percentage of shoots. The highest percentage of shoots appeared in the Bali Karet variety which was 72.22%. The treatment of bulb weight has a very real effect on the viability of shallot seedlings. The highest viability is found in the weight of large bulb (12-16 g), namely the percentage of shoots appearing 73.33%, the time to appear shoots 5.95 days, shoot length 17.13 cm, root length 13.83 cm, and total dry weight of seedlings 0.74 g, and there is an interaction of varieties and bulb weight that has a very significant effect on shoot length and total dry weight of seedlings. The best treatment combination is found in the Bali Karet variety and the weight of large bulb (12-16 g), namely the highest shoot length and total dry weight of seedlings, namely 16.67 cm and 0.74 g.
Pengaruh Konsentrasi Polyethylene Glycol (PEG) dan Jenis Media Simpan terhadap Daya Simpan Benih Kakao (Theobroma cacao L.) Noviani, Made; Darmawati, Ida Ayu Putri; Mayun, Ida Ayu
Agrotrop : Journal on Agriculture Science Vol 13 No 3 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i03.p07

Abstract

Effect of Polyethylene Glycol (PEG) Concentration and Type of Storage Media on the Shelf Life of Cocoa Seeds (Theobroma cacao L.). Cocoa seeds are recalcitrant seeds that deteriorate quickly, so special treatment is needed with the PEG concentration and type of storage media. The study started in October – November 2021. The aim of the study was to obtain the best concentration of PEG-6000 and the best type of storage media and to determine the interaction of treatments. The study used a Factorial Completely Randomized Design (CRD). The first factor is the concentration of PEG-6000 (P) with 4 levels: P0 = PEG 0% (control); P1 = PEG 15%; P2 = PEG 30% and P3 = PEG 45%. The second factor is the type of storage media (S) with 3 levels: Sk = without storage media (control); Sg = Sawdust and Ss = Husk Charcoal. The results showed that the concentration of PEG (P) had a very significant effect on the variables of healthy seeds, moldy seeds in storage and germination rate. The best results were found at PEG 45% with the highest germination rate of 10.19 sprouts/day. The type of storage media (S) had a very significant effect on the variables of healthy seeds, germinated seeds, moldy seeds in storage, seed moisture content after storage and germination rate. The highest results on husk charcoal media in seed moisture content variable after storage was 39.11%, seeds germination in the nursery was 9.70%, germination rate was 11.98 sprouts/day and hypocotyl length was 2.54 cm and there was an interaction between healthy seeds, germinated seeds and moldy seeds in storage. The best results were found at a concentration of PEG 45% with husk charcoal (P3Ss) media with the highest result of healthy seeds in storage at 9.77% and germination of seeds in storage at the lowest 0.71%.
Pengaruh Suhu Awal Air dan Lama Perendaman terhadap Viabilitas dan Vigoritas Benih Bunga Gumitir (Tagetes erecta L.) Dewi, Ni Putu Elvina Kusuma; Wijana, Gede; Darmawati, Ida Ayu Putri
Agrotrop : Journal on Agriculture Science Vol 14 No 2 (2024)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2024.v14.i02.p02

Abstract

The effect of the Initial Temperature of the Water and the soaking time on Viability and Vigority of Marigold Flower Seeds (Tagetes erecta L.). Marigold is a floral ornamental plant, which is potentially marketed in Indonesia especially in Bali. Marigold is multiplied through seeds, so the quality of seeds is highly observed. An uneven flare and a low flare power can affect the growth of marigold. This research aims to find out the initial temperature, the soaking time and interaction influences the viability and vigor of the flower seed. The research began in August-October 2023, which is located at the Laboratory of Plant ?reeding and Seed Technology, Agroecology Studies Program, Faculty of Agriculture University of Udayana. This research used a Completely Randomized Factorial Design, namely the initial temperature treatment consisted of 3 levels (30?, 40?, 50?), and the soaking time consisted of 4 levels (30, 60, 90 and 120) minutes. The results of the study showed a significant interaction influencing the viability and vigor of marigold seeds, where the initial temperature of 40°C with 30 minutes of immersion is the best interaction, with germination percentage (86%), maximum potential growth rate (92%), simultaneous growth (75.33%), the percentage growth rate (11.61%/etmal) and seed vigor indeks (5199,47).
Intraspecific SSR Marker Screening for Detection of Dendrobium crumenatum Mutants Generated from In Vitro Gamma Irradiation Dwiyani, Rindang; Sanjaya, I Putu Wahyu; Yuswanti, Hestin; Darmawati, Ida Ayu Putri; Suada, I Ketut; Manullang, Prila Kartika; Fitriani, Yuyun
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.89896

Abstract

Determination of D. crumenatum mutant obtained from in vitro mutation breeding needs a long time due to its long-life cycle. SSR molecular markers can be used for early mutant detection. Specific SSR markers developed in D. crumenatum are not yet available. Alternative published SSR markers were developed from D. catenatum. The aims of this study are to screen the most informative SSR markers generated from D. catenatum tested in irradiated D. crumenatum population and to determine the gamma irradiation dose resulting the most mutants. Ten SSR markers were randomly selected and tested in 25 individuals of D. crumenatum plantlets irradiated with several doses (0, 5, 10, 15, and 20 Gy; 5 plantlets each dose). The result showed 7 of 10 primers were polymorphic and the other three were  monomorphic. All seven polymorphic primers can be used to identified intraspecific variation in the D. crumenatum mutant population. Markers dnsr28 and dnsr98 were the most informative, with the highest polymorphic information content (PIC) value of 0.5. Irradiation D. crumenatum protocorms using 10-15 gray doses were detected as the highest mutant percentage obtained up to 100% in the sample tested. This resulting marker information can be used to screen wider mutant population to decrease the non-mutant individuals in the population for maintenance and cost efficiency. The 10-15 Gy can be used as reference doses for gamma irradiation in 3 months old D. crumenatum protocorm materials. 
Co-Authors - Afrizal A.A.N.B. KAMANDALU AGUNG WIDYA ANTASARI DEWI ANAK AGUNG MADE ASTININGSIH ANAK AGUNG NGURAH DHARMA SEPUTRA AStiningsih, Ana Agung Made Cokorda Gede Alit Semarajaya Dewi, Ni Putu Elvina Kusuma DIMAS MANDALA PUTRA DINI WIRDASARI Febriana, I Gede GEDE WIJANA Gusti Ngurah Alit Susanta Wirya H. Yuswanti HESTIN YUSWANTI I GEDE PUTU WIRAWAN I Gusti Alit Gunadi I GUSTI ALIT GUNADI I Ketut Sardiana I Ketut Suada I MADE AGUS KRISNA I MADE RAI YASA I Made Sukewijaya I NYOMAN GEDE ASTAWA I NYOMAN RAI I PUTU DIAN PRATAMA I WAYAN SUNANJAYA I.G.A. Gunadi IDA AYU ASTARINI IDA AYU MAYUN Ida Ayu Sri Dewi Adriani IDA BAGUS KADE SUASTIKA K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KETUT BUDI SUSRUSA KETUT SUADA Ketut Suada Khusni, Mazroatul Maheswari, Mea Aprilia Manullang, Prila Kartika Mulyani, Iluh Sri N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI NENGAH DARMIATI NI NYOMAN ARI MAYADEWI NI PUTU SUTAMI NI WAYAN SURIANTARI Noviani, Made PUTRI REZKY Redana, I Made Basma Retno Kawuri Rindang Dwiyani S.A.N. ARYAWATI Sanjaya, I Putu Wahyu Setora, Ketut Weda TRISNA AGUNG PHABIOLA Y. Fitriani Yuyun Fitriani