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RESPON PERTUMBUHAN PICHIA MANSHURICA DAN RHODOSPORODIUM PALUDIGENUM PADA BERBAGAI MEDIA BASAL SEBAGAI PENENTU UNTUK PROSES ISOLASI PROTOPLAS Wijanarka, Wijanarka; Sutariningsih, Endang; Dewi, Kumala; Indrianto, Ari
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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ABSTRAK Pertumbuhan mikroorganisme biasanya ditunjukkan dengan adanya pertambahan jumlah sel atau masa sel yang sedang tumbuh. Pertumbuhan mikroorganisme dipengaruhi oleh faktor lingkungan hidupnya, salah satunya medium pertumbuhan. Medium tersebut sangat menentukan tingkat keberhasilan umur kultur dan profil fase pertumbuhan yang sangat penting pada saat isolasi protoplas. Tujuan penelitian ini adalah kinetika respon dan profil pertumbuhan Pichia manshurica dan Rhodosporodium paludigenum Pada Berbagai Media Basal. Metode yang digunakan dalam penelitian ini adalah eksperimental. Penelitian ini dilakukan pada bulan Januari-Februari 2011 di Laboratorium Mikrobiologi FMIPA UNDIP Semarang. Khamir Pichia manshurica dan Rhodosporodium paludigenum ditumbuhkan pada media basal MEB (M1), TEB (M2), ME (M3) dan YPD (M4) serta dilakukan pengamatan pertumbuhan setiap 6 jam selama 42 jam. Tahap berikutnya dilakukan studi analisis kinetika pertumbuhan khamir pada media basal yang berbeda. Hasil penelitian menunjukkan bahwa media YPD (M4) mempunyai kecepatan pertumbuhan (?) tertinggi (0.2086 mg/jam) dan waktu generasi terpendek 3.3236 (menit) pada jam ke-18, sedangkan Rh. paludigenum mempunyai nilai ? sebesar 0.2751 (mg/jam) dan g sebesar 2.5197 (menit). Kesimpulan penelitian ini adalah media YPD (M4) dapat digunakan untuk pertumbuhan Pichia manshurica dan Rhodosporodium paludigenum serta dapat digunakan untuk media isolasi protoplas Kata Kunci: Pertumbuhan, media basal, P. manshurica dan R. paludigenum

Intergenus Protoplast Fusion between Pichia manshurica and Rhodosporidium paludigenum to Increase the Production of Inulinase Wijanarka, Wijanarka; Soetarto, Endang Sutariningsih; Dewi, Kumala; Indrianto, Ari
Makara Journal of Science Vol. 18, No. 4
Publisher : UI Scholars Hub

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The purposes of this study was to identify the optimum concentration of the lytic enzyme Glucanex for protoplast isolation and to conduct fusion for the purpose of increasing inulinase production. The study performs the protoplast fusion technique using Pichia manshurica and Rhodosporidium paludigenum. Protoplast fusion consists of a series of stages: protoplast isolation, protoplast fusion, protoplast regeneration, and analysis of hybrid fusion results. Protoplast isolation and fusion success rate are determined by various factors, including age of the culture, media type, and type of lytic enzymes used. Hybrid results were analyzed using a fungicide as a marker and measuring specific growth rate (µ) of the hybrid compared with parental growth rates. Results demonstrated that a concentration of 4 mg/mL of Glucanex produces the greatest number of protoplasts, 7.2 x 1010 (cell/mL) for P. manshurica and 8.8 x 1010 (cell/mL) for Rh. paludigenum. The results of analysis of hybrid fusions indicate that the study has identified a new fusant, called fusant F4. Fusant F4 is capable of producing the highest inulinase, 0.6892 IU, compared with parentals P. manshurica, 0557 IU, and Rh. paludigenum, 0.3263 IU. Fusant F4 has specific growth rate (µ) of 0.3360/h and generation time (g) of 2.0629 h.

Ideology on Children Formula Milk Television Commercials dewi, kumala
Mudra Jurnal Seni Budaya Vol 39 No 1 (2024)
Publisher : Institut Seni Indonesia Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31091/mudra.v39i1.2558

Television advertising for children's formula milk is also a text produced from a certain ideological point of view, in the sense that advertising not only functions as a marketing medium for dairy products but also conveys ideology that can influence the mindset and decisions of the audience. This study focuses on analyzing the ideology behind television commercials for children's formula milk. The research aims to find out and provide an explanation of ideology in television commercials for children's formula milk. The method in this research is qualitative, with a textual analysis approach. The primary data is in the form of recordings of ten advertisements for formula milk products aired by several Indonesian national television stations. Secondary data was obtained through observation, literature study, online data searches, and questionnaires on the public as television viewers/consumers/parents. The results of the study show that the ideology behind the ten television advertisements for children's formula milk includes the ideology of idealism and consumerism.

Development of biology module on tissue culture material Dewi, Kumala; Mellisa, Mellisa
JURNAL ATRIUM PENDIDIKAN BIOLOGI Vol 8, No 2 (2023): Atrium Pendidikan Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/apb.v8i2.14995

This study aims to produce modules on tissue culture material, which are valid. This research has three stages; analysis, design, and development. The instruments used were validation sheets and response questionnaires. The sampling technique is random. Retrieval of data using validation sheets and questionnaires. Data were analyzed using the scale method with a Likert modification. The validation results get an average of 87% (very valid). This module was well received by students in three schools with an average of 79.75% (good). Thus, the average percentage of all validators is 87.50% (very valid), and it is stated that the learning media developed are very valid.

Increasing Productivity in Planting Indigofera Tinctoria Plant Seeds as Producers of Natural Dyes in Pacarejo Village, Semanu, Gunung Kidul, D.I. Yogyakarta Supriadianto, Supriadianto; Rahayuningsih, Edia; Dewi, Kumala; Pamungkas, Mukmin Sapto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 9, No 4 (2023): Desember
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.80805

This investigation was conducted in Pacarejo Village with the participation of thirty people. This activity was a follow-up to the second year of research, with the objectives of: 1) Increasing participation and strengthening the commitment of Gapoktan of Sembada, Pacarejo Village by planting Indigofera tinctoria as an alternative plant with a fixed price that can improve people's income, 2) Realizing additional land, provision of seeds, and matrices for planting Indigofera tinctoria plants to meet the availability of sustainable raw materials, and 3) Providing assistance in care. The expected outputs to achieve an independent village are as follows: 1) Realization of a Cooperation Agreement for planting Indigofera tinctoria on at least 2 hectares, 2) Development of a planting system, plant care, and harvesting methods with optimal and sustainable quality, and 3) Availability of natural dye raw materials extracted from Indigofera tinctoria leaves that meet raw material quality standards and are sustainable. This research effectively improved the well-being of the residents of Calonejo Village, particularly the Gapoktan Sembada, Pacarejo Village by increasing the Indigofera plant nursery productivity of farmers. This research was successful due to the conclusion of a cooperation agreement to plant Indigofera tinctoria on an area of 3 hectares, the establishment of a planting system, plant care, and harvesting methods with optimal and sustainable quality, and the availability of raw materials for natural dyes extracted from Indigofera tinctoria leaves with optimal and sustainable quality. Information session, Group Discussion Forums (FGD), Training, and Direct Assistance methods are used.

Growth and Development of Winged Bean (Psophocarpus tetragonolobus (L.) DC.) Treated with Paclobutrazol Wijayanti, Jihan Sekar; Dewi, Kumala
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86238

Winged bean (Psophocarpus tetragonolobus (L.) DC.) is one of the tropical legumes commonly grown for vegetable in Indonesia. Winged bean is a kind of plants that growth on vine so that for cultivation it requires stakes or awnings. It is known that paclobutrazol is a growth retardant that acts by inhibiting gibberellin biosynthesis and application of paclobutrazol could make plant become semidwarf or even dwarf. This study was aimed to evaluate the effect of paclobutrazol on growth, development, some phytochemicals content and yield of winged bean plants. This study used a Completely Randomized Design (CRD) with one factor, namely paclobutrazol, which was applied at four different concentrations, namely 0 ppm (control), 25 ppm, 50 ppm, 75 ppm or 100 ppm. Three replicates were made for each treatment. The results showed that paclobutrazol significantly decreased plant height, number of leaves, leaf area, number of pods per plant, pod length, fresh weight of fruit, levels of vitamin C and protein in the pods, but increased the leaf chlorophyll content and stomata density on the abaxial (lower) leaf surfaces. Paclobutrazol showed its effect on accelerating flowering time at a concentration of 50 ppm.

Seed germination and growth of Joseph’s coat (Amaranthus tricolor L.) following exposure with Naphthalene-1-Acetic Acid (NAA) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) Ilmam, Hadyan Pratama Lutfi; Dewi, Kumala
Jurnal Ilmu Pertanian Vol 10, No 1 (2025): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.88687

Amaranthaceae is a family of plants that can be used as vegetables and medicinal herbs. Amaranthus tricolor L. is commonly cultivated because it has fast growth rate and short life cycle that can be boosted by growth regulators such as auxins. A. tricolor L. is commonly cultivated because it has a fast growth rate and short life cycle. Growth regulators, such as auxins, can boost the growing process. This research aimed to study the effects of Naphthalene-1-Acetic Acid (NAA) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) on the seed germination and growth of A. tricolor L. and to determine effective concentration of NAA or 2,4-D application to A. tricolor L. This research was arranged in a completely randomized design with exogenous hormones application as treatments. The treatments consisted of various concentrations of NAA and 2,4 D (0 ppm, 10 ppm, 20 ppm, 40 ppm, and 80 ppm) applied to A. tricolor L. plants every two weeks. Germination test of A. tricolor L. was carried out for 14 days, and the application of NAA and 2,4-D on A. tricolor L. plant was given for 56 days. Observations were made on the plant height, fresh and dry weight, stomatal density, and the content of chlorophyll and carotenoid. Data analysis was conducted using one-way analysis of variance and Duncan Multiple Range Test (DMRT) with significance level of 5%. NAA treatment delayed seed germination by one day compared to control, while 2,4-D treatment inhibited germination for several days with the higher concentration of 2,4-D applied, the greater inhibition of seed germination. NAA of 10 ppm increased plant height, fresh and dry weight, chlorophyll content, and leaf area of A. tricolor L. The application of NAA and 2,4-D reduced stomatal density and carotenoid content of A. tricolor L., with greater effects at higher concentrations of synthetic auxins. This research concluded that NAA or 2,4-D inhibited germination of A. tricolor L. seeds, NAA of 10 ppm effectively increased plant growth and chlorophyll content, but higher NAA concentrations inhibited growth. Application of 2,4-D with concentrations above 40 ppm could be lethal for A. tricolor L.

Seed germination and growth of Joseph’s coat (Amaranthus tricolor L.) following exposure with Naphthalene-1-Acetic Acid (NAA) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) Ilmam, Hadyan Pratama Lutfi; Dewi, Kumala
Jurnal Ilmu Pertanian Vol 10, No 1 (2025): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.88687

Amaranthaceae is a family of plants that can be used as vegetables and medicinal herbs. Amaranthus tricolor L. is commonly cultivated because it has fast growth rate and short life cycle that can be boosted by growth regulators such as auxins. A. tricolor L. is commonly cultivated because it has a fast growth rate and short life cycle. Growth regulators, such as auxins, can boost the growing process. This research aimed to study the effects of Naphthalene-1-Acetic Acid (NAA) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) on the seed germination and growth of A. tricolor L. and to determine effective concentration of NAA or 2,4-D application to A. tricolor L. This research was arranged in a completely randomized design with exogenous hormones application as treatments. The treatments consisted of various concentrations of NAA and 2,4 D (0 ppm, 10 ppm, 20 ppm, 40 ppm, and 80 ppm) applied to A. tricolor L. plants every two weeks. Germination test of A. tricolor L. was carried out for 14 days, and the application of NAA and 2,4-D on A. tricolor L. plant was given for 56 days. Observations were made on the plant height, fresh and dry weight, stomatal density, and the content of chlorophyll and carotenoid. Data analysis was conducted using one-way analysis of variance and Duncan Multiple Range Test (DMRT) with significance level of 5%. NAA treatment delayed seed germination by one day compared to control, while 2,4-D treatment inhibited germination for several days with the higher concentration of 2,4-D applied, the greater inhibition of seed germination. NAA of 10 ppm increased plant height, fresh and dry weight, chlorophyll content, and leaf area of A. tricolor L. The application of NAA and 2,4-D reduced stomatal density and carotenoid content of A. tricolor L., with greater effects at higher concentrations of synthetic auxins. This research concluded that NAA or 2,4-D inhibited germination of A. tricolor L. seeds, NAA of 10 ppm effectively increased plant growth and chlorophyll content, but higher NAA concentrations inhibited growth. Application of 2,4-D with concentrations above 40 ppm could be lethal for A. tricolor L.

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