Article Per Year (5 Year)
p-Index From 2020 - 2025
2.87
P-Index
This Author published in this journals
All Journal Majalah Forum Teknik UGM Jurnal Sain Veteriner Jurnal Teknoin Seminar Nasional Aplikasi Teknologi Informasi (SNATI) Jurnal Ergonomi Indonesia (The Indonesian Journal of Ergonomic) J@TI (TEKNIK INDUSTRI) Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Jurnal Teknosains Jurnal Teknologi Rekayasa Mesin Journal of Engineering and Management in Industrial System Journal of Medical Physics and Biophysics The Indonesian Journal of Dental Research Majalah Kedokteran Gigi Indonesia Jurnal Ilmiah Teknik Industri Dental Journal (Majalah Kedokteran Gigi) Atrium : Jurnal Arsitektur Jurnal Sistem Teknik Industri Dinamika Kerajinan dan Batik : MAJALAH ILMIAH Jurnal Kreatif : Desain Produk Industri dan Arsitektur Jurnal Material Teknologi Proses: Warta Kemajuan Bidang Material Teknik Teknologi Proses Journal of Material Processing and Characterization (JMPC) Journal of Fibers and Polymer Composites Proceeding of International Conference Health, Science And Technology (ICOHETECH) Prosiding Seminar Nasional Unimus
Rini Dharmastiti
Department Of Mechanical And Industrial Engineering, Universitas Gadjah Mada, Yogyakarta, Indonesia
Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Electrical & Electronics Engineering Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

Published : 37 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Jurnal Sain Veteriner

 1 
Optimization of Tissue Decellularization Method Based on Macroscopic and Microscopic Observation in The Sheep Peripheral Nerves Fajar Shodiq Permata; Rina Susilowati; Rini Dharmastiti; Muhammad Mirandy Pratama Sirat Mirandy Pratama Sirat; Pipin Dwi Kartikasari
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13252.092 KB) | DOI: 10.22146/jsv.2606

Abstract

Animal graft tissue  (xenograft) was developed  to replace the limited supply of human graft tissue (allograft). Peripheral nerve graft tissue  is needed to replace  the damage. Swine is the most developed source of tissue donor for the preparation of acellular tissue because a lot of livestocks population and its extracellular matrix components similar to human collagen. Swine xenograft development would be an obstacle in Indonesia because of socio-cultural so that sheep used as swine donor replacement. Sheep cartilage tissue acellular induced less human inflammatory mediators than swine. Xenograft is necessary to decellularizebefore implantation into human that results in only extracellular matrix. However, decellularization process varies depending on the species and methods so that it is needed a preliminary study to get the best decellularization method for sheep peripheral nerve specimens. Five samples of sheep Ischiadicus  nerves were conducted decellularization various processes and one sample of fresh nerve (control). Decellularization methods were 24 hours shaking, tissue soaking and perfusion for both 14 days and 17 days, respectively. Decellularization solution was 0.1% SDS-EDTAin PBS. Post decellularization, samples were observed macroscopically, fixed, HE staining of histopathologic examination for microscopic examination. The data were analyzed descriptively. At the macroscopic observation of post decellularization tissue showed white, and samples of soaking method showed softer consistency than that of shaking and perfusion. Microscopic examination showed that samples were not being completed decellularization at shaking method. There was destruction of collagen fibers of perineurium and endoneurium in 17 days of soaking and perfusion methods, while 14 days soaking and perfusion methods showed that samples were being completed decellularization and both perineurium and endoneurium collagen were still good. Conclusion of this study is that 14 days soaking and perfusion decellularization methods are better than that of 24 hours shaking method and 17 days of soaking and perfusion methods for sheep decellularization peripheral nerve.  
Co-Authors A.A. Ketut Agung Cahyawan W Abdullah, Muhammad J.Q. Amalia Kiswandari Andi Rahadiyan Wijaya, Andi Rahadiyan Ardiyanto Ardiyanto Arfan Fadilah Aria Fransiska Bambang Moyoretno Bambang Moyoretno, Bambang Benidiktus Tulung Prayoga Budi Arifvianto Catur Septommy Chancard Basumerda Darmanto Darmanto Dewi Arsih Sulistiani Dhirga Harisa Fadilah, Arfan Fajar Shodiq Permata Handoko Handoko Hapsoro Agung Jatmiko Hilya Mudrika Arini Ibnu Al-Taufiq Indah Pratiwi Indarto Indarto Indriany, Emmy Kurniasari, Fitri Leny Yuliatun Lientje Setyawati Lientje Setyawati Lusiana Pane Marcellino Aditya Mahendra Marsetyawan HNE Soesatyo Melita Puspitasari Mohammad Juffrie, Mohammad Muhamad Adhitya Nugroho Muhammad Mirandy Pratama Sirat Munim, Yulianda Sakinah Muslim Mahardika Nur Kaliwantoro Nuryono Nuryono Pararta, Wilhelmus A. Pipin Dwi Kartikasari Purnomo Purnomo Purnomo Purnomo Rahadyan Magetsari Rahmatika S, Devi Dwi Resti Efrata Eka Rethy Den Rina Susilowati Rusdaniaji, Danarwan Salim, Urip A. Salim, Urip Agus Salsabila, Zulfi Fathiya Saputro, Oktiyanto A. Setiyani, Rezki Siti Lestariningsih Siti Lestariningsih, Siti Siti Sunarintyas Sri Budi Barunawati Suprihatin Suprihatin Suyitno Suyitno Suyitno Suyitno Suyitno Suyitno Taufik Ramadhan Fitrianto Taufik Ramadhan Fitrianto W. Widjijono Wardoyo, Christianus Pigun Widasari, Putri Pratiwi Wijaya, Andi Rahadyan Yulianda Sakinah Munim