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Journal : Jurnal Teknosains

ROLE OF REDUCING CELL LEAKAGE IN CELL CULTURE USING LARGE PORE SIZE PERMEABLE MEMBRANE Nur Kaliwantoro; Marsetyawan HNE Soesatyo; Indarto Indarto; Mohammad Juffrie; Rini Dharmastiti; Suprihatin Suprihatin
Jurnal Teknosains Vol 6, No 1 (2016): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.27192

Abstract

Permeable membranes are widely used in many in vitro studies using cell culture. Some cell leakage are often occurs when using permeable membrane with large pore size. Unfortunately the use of permeable membrane with smaller pore size in permeability studies faces some difficulties due to its small pore size and pore density. Recent study provides the protocol in using permeable membrane with large pore size with smaller cell leakage. Vero cell line (CCL-81, ATCC) was used and culture on polyester permeable membrane with 3 µm pore size. Visualization using inverted microscope was used to analized the cell leakage on the permeable membrane. Parallel plate flow chamber was used to analized the permeability performance of the Vero cell cultured on the permeable membrane. The result showed that the current technique is significant in reducing cell leakage of the cell cultured on large pore size of permeable membrane. The same results were found in using polyester and polycarbonate permeable membrane.
PENGEMBANGAN ALAT UKUR EVALUASI DAN PERANCANGAN PRODUK KURSI RODA Hapsoro Agung Jatmiko; Rini Dharmastiti
Jurnal Teknosains Vol 7, No 2 (2018): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.28222

Abstract

A wheelchair is one of the mobility aids for people with disabilities. Availability of wheelchair type is very decisive for users based on the limitations they have. The problem felt by users in Indonesia today, especially in Yogyakarta, is that users have not got the right kind of wheelchairs with the needs, with certain limitations. This study aims to develop a wheelchair evaluation and to know the user's expectation of the wheelchair design. This study shows that there are problems due to the users not getting a wheelchair that suits their needs. The wheelchair design that the users want are the wheelchair with 3 wheels and have new feature.
Coloration study heat-cured polymer materials for denture base Setiyani, Rezki; Salim, Urip Agus; Mahardika, Muslim; Dharmastiti, Rini; Arifvianto, Budi
Jurnal Teknosains Vol 14, No 2 (2025): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.104274

Abstract

The color of denture base material is important in dentistry to achieve a natural gingival aesthetic. No universal standard for denture base color, due to it difficult for dentists and dental laboratory technicians to achieve consistent results. This study proposes a method for identifying the color of artificial gums made from heated cure polymers with coloring agents. This study also examined the effect adding color agent on the hardness of denture base material and the effect of artificial saliva immersion on coloring. New coloring agents, namely pink (P), red (M), and purple (U), were added to create new colors in this study. Seven specimens from light to dark colors were made. The resulting specimens were photographed and analyzed using Adobe Photoshop software to obtain the L*, a*, and b* values for each specimen, which were then analyzed using the CIELAB formula. The results of the material hardness test showed a significant change between the immersion and non-immersion groups (p-value 0.00<0.05), while the group between 10-day and 20-day immersion showed no significant change (p-value 0.65>0.05). In materials without added color, the hardness value is 85.3 - 86.3 HSD, while in materials with added color, the hardness value increases to 85.5 - 87.7 HSD. The results of the saliva immersion test showed changes in the range of 2.51 - 5.98 for 10 days of immersion and 0.85 - 4.22 for 20 days of immersion. Based on these results, most of the color changes are still below the clinical acceptance threshold of less than 4.1. Therefore, it can be concluded that the color changes that occur after soaking are still clinically acceptable.
Noise analysis of grinding machine in the material cutting process Dharmastiti, Rini; Wijaya, Andi Rahadyan; Kurniasari, Fitri
Jurnal Teknosains Vol 14, No 2 (2025): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.105448

Abstract

The handheld grinding machine used for cutting materials has generated noise that may affect the operator and individuals nearby. The noise produced by this machine needs to be analyzed to prevent potential health impacts. This study aims to measure the noise levels the handheld grinding machine generates while cutting materials such as ceramic, metal, and natural stone. Noise measurements were taken at the noise source and distances of 2 meters, 3 meters, and 4 meters from the source. A sound level meter was used for the noise measurements. The study's results indicate that the type of material being cut using the handheld grinder does not significantly affect the noise level produced. The highest noise level was recorded when cutting the plate, with an equivalent continuous sound level (Leq) of 91.6 dB at the source. The lowest noise level was recorded when cutting ceramic material, with a Leq of 89.7 dB. Statistical tests on the distance and measurement locations at 2 meters, 3 meters, and 4 meters from the source showed that the distance does not significantly affect the noise level. At distances of 2 meters and 3 meters, the average Leq for all materials was the same, ata 94.2 dB, while at 4 meters, the average Leq was slightly lower, at 93.9 dB.
Fabrication of single-axis prosthetic foot using 3d printing and resin infusion method Rahmatika S, Devi Dwi; Arifvianto, Budi; Dharmastiti, Rini
Jurnal Teknosains Vol 14, No 2 (2025): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.105483

Abstract

A prototype of a single-axis prosthetic foot is proposed as an alternative prosthetic foot for patients with disabilities to perform daily activities. Three leading positions within 0-64% of the gait cycle are heel strike, midstance, and toe-off positions used as boundary conditions. The Finite Element Method can analyze a model to determine the values of deformation, stress, and strain that occur in detail. This research is conducted to fabricate an optimal prosthetic foot product or prototype and determine its strength to support the weight of the user's body and provide comfort and safety before being fabricated using 3D printing and resin infusion methods. The fabrication of the prosthetic foot prototype used Fusion 360 software and continued with simulation testing using ABAQUS 6.14 software. The prototype model was designed and evaluated to obtain strength and feasibility before being fabricated with an Anycubic Photon Mono X type DLP 3D printing machine and resin infusion method. The results obtained in this study show that the prototype of a single-axis prosthetic foot can support the human body's weight with loading level P3 or 60 kg, level P4 or 80 kg, and level P5 or 100 kg according to ISO 10328. The mass of the prototype or single-axis prosthetic foot product is 657 grams, according to the criteria, not more than 1.7% of the user's total body weight, with a hardness value of 55.5 HA, and can withstand 50,000 cycles in fatigue testing.