Article Per Year (5 Year)
p-Index From 2021 - 2026
2.42
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Agro Jurnal Sumberdaya Hayati Indonesian Journal of Science and Technology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Celebes Biodiversitas : Jurnal Sains dan Pendidikan Biologi Jurnal Biologi Tropis Menara Perkebunan Jurnal Jamu Indonesia EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Indonesian Journal of Ecology and Conservation Biodiversitas Journal of Biological Diversity
Diah Ratnadewi
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

Published : 31 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 31 Documents
Search

 1   2   3   4 
PENGARUH APLIKASI HERBISIDA PADA SIMPANAN BIJI DALAM TANAH SAVANA BEKOL TAMAN NASIONAL BALURAN Nurmuliayanti Muis; Titiek Setyawati; Soekisman Tjitrosoedirjo; Y. M. Diah Ratnadewi
CELEBES BIODIVERSITAS : Jurnal Sains dan Pendidikan Biologi Vol 5, No 2 (2022): Science, Conservation, Biology Education
Publisher : Universitas Patompo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51336/cb.v5i2.435

Abstract

The dominance of broadleaf weeds after A. nilotica control resulted in a decrease in the dominance of grass species in the savanna area of Bekol BNP. Herbicide application in controlling broadleaf weeds is an effective and efficient method. However, the impact of using herbicides on seed storage in the soil is unknown, while seed storage has an important role in the sustainability of vegetation. This study used a completely randomized factorial design with three factors, the factor was soil depth, the type of herbicide, herbicide concentration and control (without herbicide application). The effect of herbicide application on seed germination was analyzed by ANOVA. The results of this study indicated that the application of herbicides had no significant effect on seed germination in soil (P>0.05). So, the use of triclopyr and fluroxypyr herbicides could be considered as one of the measures in controlling broadleaf weeds in the Savama area of BNP.
Analisis Residu Herbisida dalam Pengendalian Gulma Berdaun Lebar Di Savana Bekol Taman Nasional Baluran Saputri, Rinny; Ratnadewi, YM Diah; Tjitrosoedirdjo, Soekisman; Setyawati, Titiek
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 8 No 1 (2023): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekotonia.v8i1.4081

Abstract

The research was conducted in the savanna Bekol - Baluran National Park, East Java to find the best rate of herbicide to control broadleaved weeds in the savanna and to estimate the soil residue of herbicide used. The experimental design was a randomized block with six treatments and four replications. The treatments were herbicide applications consisted of (1) the rate of triclopyr at 670 g a.i ha-1 (TA), (2) rate of triclopyr at 1340 a.i ha-1 (TB), (3) the rate of fluroxypyr at 200 g a.i ha-1 (FA), (4) rate of fluroxypir at 400 g a.i ha-1 (FB), (5) Weeding Manual (PM) and (6) Control (K). The application of triclopyr at 670 g ai ha-1 was among the best of treatments applied. Sampling of soil at day 0 and day 28 after application indicated the residue of triclopyr 670 g ai ha-1 was 0.16 ppm and DT50 = 14 d.
Regenerasi Tanaman Kitolod (Hippobroma longiflora (L.) G.Don) pada Kultur In Vitro: Plant Regeneration of Kitolod (Hippobroma longiflora (L.) G.Don) in In Vitro Culture ., Sumaryono; Nur`Aeni, Fitri; Ratnadewi, Diah
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.14-19

Abstract

Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant. Its flower is widely used as a traditional medicine.When this plant is utilized more intensively, there may be a shortage of the plant due to the lack of seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials. The experiments were arranged using a completely randomized design with two treatment factors and 10 replications for all experiments except in shoot rooting. Leaves and petioles were used as explant sources. Various combinations of benzilamino purine (BAP) and naphtalene acetic acid (NAA) were applied. Leaf explants in Murashige and Skoog (MS) medium enriched with 1 mg/L BAP and 0.1 mg/L NAA combination produced the highest number of adventitious shoots per explant, but 2 mg/L BAP + 0.1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots, and presented 75% of successful rate over the acclimatization period. The best rooting was provided by MS medium added with 0.5-1.0 mg/L NAA.
Plant-Derived Exosome-like Nanoparticles from Emprit Ginger (Zingiber officinale var. Amarum) and Its Potential Metabolite as Functional Food Ingredients Rukmi, Paramita Sadha Dayinta; Ratnadewi, Diah; Wijaya, Christofora Hanny
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.181-195

Abstract

The Plant-Derived Exosome-like Nanoparticles (PDENs) are nano-sized vesicles secreted by plants that carry various bioactive compounds and have shown promise for use in functional food applications. This study investigated PDENs isolated from emprit ginger (Zingiber officinale var. Amarum), a local ginger variety renowned for its health-promoting properties. PDENs were extracted from rhizomes harvested at 8, 10, and 12 months, and analyzed for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity. The best-performing sample based on these parameters was selected for further metabolite profiling using LC-QTOF-MS. A total of 41 compounds were identified from the selected GDEN and ginger extract samples—32 compounds in the GDEN and 24 in the extract. These compounds belong to various groups, including amino acids, flavonoids, phenolics, lipids, terpenoids, vitamins, and others. Compound identification was based on public databases and literature concerning their potential as functional food ingredients. Among these, amino acids were the most abundant group in the GDENs, whereas phenolics were the dominant group in the ginger extract. This study underscores the potential of ginger-derived exosome nanoparticles as a rich source of bioactive compounds, supporting their further exploration and application in the development of functional food products. The balanced metabolite profile observed in GDENs highlights their unique advantages over conventional extracts. These findings reinforce the potential of emprit GDENs as promising candidates for functional food development.
Manipulasi Nutrien dan Zat Pengatur Tumbuh untuk Meningkatkan Produksi dan Kualitas Buah Stroberi (Fragaria x ananassa) Kultivar BAT-1 Tjahjoleksono, Aris; Gustania, Leolita; Ratnadewi, Diah
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 2 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.2.63-70

Abstract

Stroberi kultivar BAT-1 semakin jarang dibudidayakan oleh petani Indonesia karena rasa buahnya kurang manis, padahal kultivar tersebut mampu beradaptasi dengan kondisi lingkungan tropis. Hal tersebut disebabkan perbaikan kualitas dan produktivitas stroberi adaptif itu masih sulit dilakukan dengan praktik pertanian biasa. Tujuan penelitian ini adalah untuk meningkatkan produksi dan kualitas buah stroberi BAT-1, yaitu ukuran, warna, rasa manis dan umur simpan, dengan memodifikasi nutrien dan manipulasi zat pengatur tumbuh (ZPT). Penelitian ini dilaksanakan pada Agustus – Desember 2021 (musim hujan) dan Februari – Juni 2022 (musim kemarau), di kaki Gunung Halimun-Salak, Kabupaten Bogor. Penelitian menggunakan rancangan acak lengkap faktorial: Perlakuan tahap pertama berupa pemberian kalium dan kalsium, dan diukur parameter vegetatifnya. Untuk tahap kedua diberikan perlakuan asam indol butarat dan brassinolida, dan diukur parameter generatifnya. Hasilnya, perlakuan kalium dan kalsium mempercepat dan mempersingkat rentang waktu berproduksi tanaman; dan modifikasi nutrien yang berkombinasi dengan ZPT mampu meningkatkan bobot buah, kandungan antosianin buah, nilai kemanisan serta menurunkan nilai total asam. D3I3B2 (PPC + CaCl2 2 g L-1 pada daun dan ZPT IBA 3 mM + BL 0.2 µM pada buah) merupakan perlakuan terbaik, di kedua musim tanam. Brassinolida (0.2 µM) mampu memperlama daya simpan buah setelah panen.-1Kata kunci: Brassinolide, brix, IBA, kalium, kalsium.
Antibacterial Activity of Quinoline Alkaloids from Cinchona ledgeriana Cell Suspension Cultures Againts Staphylococcus aureus and Escherichia coli Hasibuan, Yustiny Andaliza; Ratnadewi, Diah
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10779

Abstract

Kina (Cinchona ledgeriana) is a member of the Rubiacea family of the genus Cinchona that contains a secondary metabolite called quinoline alkaloid. In vitro cell culture has been shown to produce secondary metabolites, by cultivating cells in a nutrient-rich liquid medium under suitable environmental conditions. The aim of this study was to determine the antibacterial activity of quinoline alkaloids from Kina cell cultures against Escherichia coli and Staphylococcus aureus. Quinoline alkaloid extracted from Kina cell culture was tested for its antibacterial activity using the well methode. Antibacterial tests revealed that quinoline alkaloids produced greater inhibition zone diameters against S. aureus (7.33 mm) and E. coli (1.66 mm) compared to solutions of pure quinine and quinidine. However, the inhibition of quinoline alkaloids against S. aureus and E. coli was still very low because they only had an inhibition zone with a diameter of <10 m.
Isolation, Characterization, and Identification of Biosurfactant-Producing Bacteria from Palm Oil Mill Effluent Jezila Amana Nousra Atsemani; Nisa Rachmania Mubarik; Yuliana Maria Diah Ratnadewi
Jurnal Ilmu Pertanian Indonesia Vol. 31 No. 3 (2026): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.31.3.426

Abstract

Biosurfactants-producing bacteria are bacteria with the ability to produce natural surfactants that can degrade oil both in soil and water. Most of these biosurfactant-producing bacteria are often found in area polluted by grease or oil like palm oil mill effluent. The purpose of this research was to isolate, characterize, and identify biosurfactant-producing bacteria from palm oil mill effluent. This research was conducted using descriptive and identification methods through a general screening using oil displacement, drop collapse, and hemolysis assay, morphological characterization, emulsification tests and molecular identification for the bacteria with the highest emulsification index. Bacteria were isolated from palm oil wastewater by dilution method. In this study, 17 isolates were obtained with 2 isolates having the highest oil spreading results (SLP2.2 and SLP1.5). Both isolates showed negative hemolysis activity and were both identified as Gram-positive bacilli. Both isolates were cultivated for biosurfactant production and subsequent extraction. Crude biosurfactant extract was successfully obtained from isolate SLP2.2 with a yield of approximately 0.08 g/L. Emulsification index value of SLP2.2 extract was 36.93 ± 0.57%. SLP2.2 was identified as Cytobacillus kochii. This research provides microbial resources for microbial enhanced oil recovery and lays a foundation for practical application, such as sustainable bio-based products development.
LOCALIZATION OF GFDD4-1 EXPRESSED PROTEIN IN Physcomitrella patens CELLS DIAH RATNADEWI
BIOTROPIA Vol. 13 No. 1 (2006): BIOTROPIA Vol. 13 No. 1 June 2006
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.934 KB) | DOI: 10.11598/btb.2006.13.1.214

Abstract

The expression of a new dehydration-related gene of Physcomitrella patens, GFDD4-I, was traced for its localization in the plant cells. This revelation is useful to predict the possible roles of the protein in plant tolerance to environmental stress. This gene was fused to  gfp  marker gene and transfected into the plant protoplasts. Under a confocal laser microscope, it was detected that the GFDD4-1 protein associated with the OFF started to generate at the cell periphery and developed more intensively inwards to cytoplasm, forming vesicles and cystemal structures or network. The protein might be membrane protein which may involve directly in membrane maintenance or cellular protection against stress conditions. Key words : Protoplast transformation, protein localization, dehydration-related gene, GFP, Physcomitrella patens
ENHANCED PRODUCTION LEVEL OF QUININE IN CELL SUSPENSION CULTURE OF Cinchona ledgeriana Moens BY PACLOBUTRAZOL Diah Ratnadewi
BIOTROPIA Vol. 20 No. 1 (2013): BIOTROPIA Vol. 20 No. 1 June 2013
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.335 KB) | DOI: 10.11598/btb.2013.20.1.291

Abstract

Quinine is one of the major alkaloids in Cinchona spp., and it is used both as a medication and as a drink additive. The plant produces most of its alkaloids in the bark after 6–8 years of age.Repeated harvests can be performed until the plant dies, but only after every 3–5 years. We tested an improved method for culturing cell suspensions of Cinchona ledgerianato investigate the possibility of increasing production of quinine. The clone QRC 315 was treated with either a growth retardant (abscisic acid or paclobutrazol) or precursor feeding of L-tryptophan. To generate stress, we applied mannitol at 5.3 g/L combined with sucrose at a lower concentration (20 g/L),and we used sucrose at 30 g/L as the control. Paclobutrazol (7 mg/L) significantly suppressed cell growth and produced the highest level of quinine (11%) after 7 weeks of culture. L-tryptophan also reduced cell growth, but without any positive effect in the production of quinoline. The highest amount of quinine per culture flask, however,  resulted in cells treated with 3 mg/L abscisic acid.
Genetic diversity of rosewood (Dalbergia latifolia) in Yogyakarta, Indonesia for plus trees selection INDIRA RIASTIWI; WITJAKSONO WITJAKSONO; DIAH RATNADEWI; ULFAH JUNIARTI SIREGAR
Biodiversitas Journal of Biological Diversity Vol. 23 No. 5 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230546

Abstract

Abstract. Riastiwi I, Witjaksono, Ratnadewi D, Siregar UJ. 2022. Genetic diversity of rosewood (Dalbergia latifolia) in Yogyakarta, Indonesia for plus trees selection. Biodiversitas 23: 2630-2639. Rosewood (Dalbergia latifolia Roxb.) or sonokeling is an important woody plant species with a high selling value. However, due to overexploitation, this species has been listed in Appendix II of CITES and its trade is strictly regulated. Therefore, the cultivation of this plant needs encouragement, and superior planting materials from selected plus trees are needed. This study aimed to examine the genetic diversity of the rosewood population and select plus trees in Yogyakarta Province, Indonesia. The plus tree selection was performed by comparing plus tree candidates with five other trees nearby in four districts, i.e. Bantul, Gunungkidul, Kulon Progo, and Sleman. As many as 61 plus tree candidates have been identified. Genetic diversity was assessed using 10 selected ISSR primers, resulting in 101 ISSR loci with average Polymorphic Loci values ??of 64.71% and Nei Heterozygosity of 0.23. The highest gene diversity (He=0.29) was found in the Kulon Progo population, and the lowest He=0.17 was in the Bantul population. The dendogram and PCA analysis put Gunungkidul and Bantul populations into one group, separated from the Sleman and Kulon Progo populations. Based on morphological and molecular analysis, six superior plus trees were obtained.
Co-Authors . Suharyo . SUMARYONO . TRIVADILA Adabiyah, Rifatul Ai Nurhasanah Husnul Izzati Anggraini Barlian Aris Tjahjoleksono Atik Choirul Hidajah C Hanny Wijaya Christofora Hanny Widjaja Dian Rahma Pratiwi Dyah Iswantini Dyah Retno Wulandari Dyah Retno Wulandari Edhi Sandra Efah FITRAMALA Elsa May Susanti ENDANG HADIPOENTYANTI Erwin Al Hafiizh Eva KHAERUNNISA Gustania, Leolita Hadi SUNARSO Hari Basuki Notobroto Hasibuan, Yustiny Andaliza Hayati MINARSIH Ika Dewi Ana Imron RIYADI INDIRA RIASTIWI Jezila Amana Nousra Atsemani Khalisa Aini Sinaga Khalisa Aini Sinaga Leksonowati, Aryani Lilis Solihat Maulidiyah Utami Muhamad Ayi Pandu Perdana Nina Ratna Djuita Nisa Rachmania Mubarik Nur Amallia Nurmuliayanti Muis Nur`Aeni, Fitri Rahmi, Putri Rifatul Adabiyah Rinny Saputri Rizka Musdalifah Amsar Rukmi, Paramita Sadha Dayinta RUSMILAH SUSENO Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirjo Soekisman Tjitrosoedirjo Soekisman Tjitrosoedirjo Sumaryono . Titiek Setyawati Titiek Setyawati Titiek Setyawati Titiek Setyawati Titiek Setyawati Tjitrosoedirdjo, Soekisman Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Triati Dewi Kencana Wungu ULFAH JUNIARTI SIREGAR Wijayanta, Ardian Nur WIRDHATUL MUSLIHATIN WITJAKSONO WITJAKSONO Witjaksono Witjaksono WOLFGANG FRANK Yohana Caecilia Sulistyaningsih Yustiny Andaliza Hasibuan Zainal Alim Mas’ud Zainal Alim Mas’ud