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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Agro Jurnal Sumberdaya Hayati Indonesian Journal of Science and Technology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Celebes Biodiversitas : Jurnal Sains dan Pendidikan Biologi Jurnal Biologi Tropis Menara Perkebunan Jurnal Jamu Indonesia EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Indonesian Journal of Jamu
Diah Ratnadewi
Biology Department, Fac. Mathematics And Natural Sciences, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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PENGARUH APLIKASI HERBISIDA PADA SIMPANAN BIJI DALAM TANAH SAVANA BEKOL TAMAN NASIONAL BALURAN Nurmuliayanti Muis; Titiek Setyawati; Soekisman Tjitrosoedirjo; Y. M. Diah Ratnadewi
CELEBES BIODIVERSITAS : Jurnal Sains dan Pendidikan Biologi Vol 5, No 2 (2022): Science, Conservation, Biology Education
Publisher : Universitas Patompo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51336/cb.v5i2.435

Abstract

The dominance of broadleaf weeds after A. nilotica control resulted in a decrease in the dominance of grass species in the savanna area of Bekol BNP. Herbicide application in controlling broadleaf weeds is an effective and efficient method. However, the impact of using herbicides on seed storage in the soil is unknown, while seed storage has an important role in the sustainability of vegetation. This study used a completely randomized factorial design with three factors, the factor was soil depth, the type of herbicide, herbicide concentration and control (without herbicide application). The effect of herbicide application on seed germination was analyzed by ANOVA. The results of this study indicated that the application of herbicides had no significant effect on seed germination in soil (P>0.05). So, the use of triclopyr and fluroxypyr herbicides could be considered as one of the measures in controlling broadleaf weeds in the Savama area of BNP.
Analisis Residu Herbisida dalam Pengendalian Gulma Berdaun Lebar Di Savana Bekol Taman Nasional Baluran Saputri, Rinny; Ratnadewi, YM Diah; Tjitrosoedirdjo, Soekisman; Setyawati, Titiek
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 8 No 1 (2023): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekotonia.v8i1.4081

Abstract

The research was conducted in the savanna Bekol - Baluran National Park, East Java to find the best rate of herbicide to control broadleaved weeds in the savanna and to estimate the soil residue of herbicide used. The experimental design was a randomized block with six treatments and four replications. The treatments were herbicide applications consisted of (1) the rate of triclopyr at 670 g a.i ha-1 (TA), (2) rate of triclopyr at 1340 a.i ha-1 (TB), (3) the rate of fluroxypyr at 200 g a.i ha-1 (FA), (4) rate of fluroxypir at 400 g a.i ha-1 (FB), (5) Weeding Manual (PM) and (6) Control (K). The application of triclopyr at 670 g ai ha-1 was among the best of treatments applied. Sampling of soil at day 0 and day 28 after application indicated the residue of triclopyr 670 g ai ha-1 was 0.16 ppm and DT50 = 14 d.
Regenerasi Tanaman Kitolod (Hippobroma longiflora (L.) G.Don) pada Kultur In Vitro: Plant Regeneration of Kitolod (Hippobroma longiflora (L.) G.Don) in In Vitro Culture ., Sumaryono; Nur`Aeni, Fitri; Ratnadewi, Diah
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.14-19

Abstract

Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant. Its flower is widely used as a traditional medicine.When this plant is utilized more intensively, there may be a shortage of the plant due to the lack of seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials. The experiments were arranged using a completely randomized design with two treatment factors and 10 replications for all experiments except in shoot rooting. Leaves and petioles were used as explant sources. Various combinations of benzilamino purine (BAP) and naphtalene acetic acid (NAA) were applied. Leaf explants in Murashige and Skoog (MS) medium enriched with 1 mg/L BAP and 0.1 mg/L NAA combination produced the highest number of adventitious shoots per explant, but 2 mg/L BAP + 0.1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots, and presented 75% of successful rate over the acclimatization period. The best rooting was provided by MS medium added with 0.5-1.0 mg/L NAA.
Plant-Derived Exosome-like Nanoparticles from Emprit Ginger (Zingiber officinale var. Amarum) and Its Potential Metabolite as Functional Food Ingredients Rukmi, Paramita Sadha Dayinta; Ratnadewi, Diah; Wijaya, Christofora Hanny
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.181-195

Abstract

The Plant-Derived Exosome-like Nanoparticles (PDENs) are nano-sized vesicles secreted by plants that carry various bioactive compounds and have shown promise for use in functional food applications. This study investigated PDENs isolated from emprit ginger (Zingiber officinale var. Amarum), a local ginger variety renowned for its health-promoting properties. PDENs were extracted from rhizomes harvested at 8, 10, and 12 months, and analyzed for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity. The best-performing sample based on these parameters was selected for further metabolite profiling using LC-QTOF-MS. A total of 41 compounds were identified from the selected GDEN and ginger extract samples—32 compounds in the GDEN and 24 in the extract. These compounds belong to various groups, including amino acids, flavonoids, phenolics, lipids, terpenoids, vitamins, and others. Compound identification was based on public databases and literature concerning their potential as functional food ingredients. Among these, amino acids were the most abundant group in the GDENs, whereas phenolics were the dominant group in the ginger extract. This study underscores the potential of ginger-derived exosome nanoparticles as a rich source of bioactive compounds, supporting their further exploration and application in the development of functional food products. The balanced metabolite profile observed in GDENs highlights their unique advantages over conventional extracts. These findings reinforce the potential of emprit GDENs as promising candidates for functional food development.
Manipulasi Nutrien dan Zat Pengatur Tumbuh untuk Meningkatkan Produksi dan Kualitas Buah Stroberi (Fragaria x ananassa) Kultivar BAT-1 Tjahjoleksono, Aris; Gustania, Leolita; Ratnadewi, Diah
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 2 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.2.63-70

Abstract

Stroberi kultivar BAT-1 semakin jarang dibudidayakan oleh petani Indonesia karena rasa buahnya kurang manis, padahal kultivar tersebut mampu beradaptasi dengan kondisi lingkungan tropis. Hal tersebut disebabkan perbaikan kualitas dan produktivitas stroberi adaptif itu masih sulit dilakukan dengan praktik pertanian biasa. Tujuan penelitian ini adalah untuk meningkatkan produksi dan kualitas buah stroberi BAT-1, yaitu ukuran, warna, rasa manis dan umur simpan, dengan memodifikasi nutrien dan manipulasi zat pengatur tumbuh (ZPT). Penelitian ini dilaksanakan pada Agustus – Desember 2021 (musim hujan) dan Februari – Juni 2022 (musim kemarau), di kaki Gunung Halimun-Salak, Kabupaten Bogor. Penelitian menggunakan rancangan acak lengkap faktorial: Perlakuan tahap pertama berupa pemberian kalium dan kalsium, dan diukur parameter vegetatifnya. Untuk tahap kedua diberikan perlakuan asam indol butarat dan brassinolida, dan diukur parameter generatifnya. Hasilnya, perlakuan kalium dan kalsium mempercepat dan mempersingkat rentang waktu berproduksi tanaman; dan modifikasi nutrien yang berkombinasi dengan ZPT mampu meningkatkan bobot buah, kandungan antosianin buah, nilai kemanisan serta menurunkan nilai total asam. D3I3B2 (PPC + CaCl2 2 g L-1 pada daun dan ZPT IBA 3 mM + BL 0.2 µM pada buah) merupakan perlakuan terbaik, di kedua musim tanam. Brassinolida (0.2 µM) mampu memperlama daya simpan buah setelah panen.-1Kata kunci: Brassinolide, brix, IBA, kalium, kalsium.
Analisis Residu Herbisida dalam Pengendalian Gulma Berdaun Lebar Di Savana Bekol Taman Nasional Baluran Saputri, Rinny; Ratnadewi, YM Diah; Tjitrosoedirdjo, Soekisman; Setyawati, Titiek
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 8 No 1 (2023): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekotonia.v8i1.4081

Abstract

The research was conducted in the savanna Bekol - Baluran National Park, East Java to find the best rate of herbicide to control broadleaved weeds in the savanna and to estimate the soil residue of herbicide used. The experimental design was a randomized block with six treatments and four replications. The treatments were herbicide applications consisted of (1) the rate of triclopyr at 670 g a.i ha-1 (TA), (2) rate of triclopyr at 1340 a.i ha-1 (TB), (3) the rate of fluroxypyr at 200 g a.i ha-1 (FA), (4) rate of fluroxypir at 400 g a.i ha-1 (FB), (5) Weeding Manual (PM) and (6) Control (K). The application of triclopyr at 670 g ai ha-1 was among the best of treatments applied. Sampling of soil at day 0 and day 28 after application indicated the residue of triclopyr 670 g ai ha-1 was 0.16 ppm and DT50 = 14 d.
Production of Secondary Metabolite Compounds of Gotu Kola (Centella asiatica) Under Salinity and Drought Stress Amallia, Nur; Mas’ud, Zainal Alim; Ratnadewi, Diah
Jurnal Jamu Indonesia Vol. 5 No. 2 (2020): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v5i2.102

Abstract

Gotu kola (Centella asiatica) is a traditional herbal plant that has been reported have a variety of pharmacological activities. The compounds of gotu kola that play a role on pharmacological activities are triterpene group compounds, namely madecasosside (MD), asiaticoside (AS), madecassic acid (AM), and asiatic acid (AA). Stress can affect the production of biomass and secondary metabolite compounds in plants. The aims of the study is to analyze the effect of salinity and drought stress on biomass and 4 compounds of triterpene in gotu kola. Harvesting is done when the plant is 8 weeks old. The yield of the biomass was analyzed and then extracted using methanol solvent to be analyzed secondary metabolite levels using the high performance liquid chromatography (HPLC) method. The lowest crop biomass was obtained at 50% of field capacity and 3,000 ppm salt content. The highest levels of MD and AS were established in conditions of 100% field capacity and 1,000 ppm salt content. The 50 and 100% field capacity and 1,000-3,000 ppm salt content did not affect the levels of AM and AA.
LOCALIZATION OF GFDD4-1 EXPRESSED PROTEIN IN Physcomitrella patens CELLS RATNADEWI, DIAH
BIOTROPIA Vol. 13 No. 1 (2006): BIOTROPIA Vol. 13 No. 1 June 2006
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.934 KB) | DOI: 10.11598/btb.2006.13.1.214

Abstract

The expression of a new dehydration-related gene of Physcomitrella patens, GFDD4-I, was traced for its localization in the plant cells. This revelation is useful to predict the possible roles of the protein in plant tolerance to environmental stress. This gene was fused to  gfp  marker gene and transfected into the plant protoplasts. Under a confocal laser microscope, it was detected that the GFDD4-1 protein associated with the OFF started to generate at the cell periphery and developed more intensively inwards to cytoplasm, forming vesicles and cystemal structures or network. The protein might be membrane protein which may involve directly in membrane maintenance or cellular protection against stress conditions. Key words : Protoplast transformation, protein localization, dehydration-related gene, GFP, Physcomitrella patens
Enhanced Production Level of Quinine in Cell Suspension Culture of Cinchona ledgeriana Moens by Paclobutrazol Ratnadewi, Diah
BIOTROPIA Vol. 20 No. 1 (2013): BIOTROPIA Vol. 20 No. 1 June 2013
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.335 KB) | DOI: 10.11598/btb.2013.20.1.291

Abstract

Quinine is one of the major alkaloids in Cinchona spp., and it is used both as a medication and as a drink additive. The plant produces most of its alkaloids in the bark after 6–8 years of age.Repeated harvests can be performed until the plant dies, but only after every 3–5 years. We tested an improved method for culturing cell suspensions of Cinchona ledgerianato investigate the possibility of increasing production of quinine. The clone QRC 315 was treated with either a growth retardant (abscisic acid or paclobutrazol) or precursor feeding of L-tryptophan. To generate stress, we applied mannitol at 5.3 g/L combined with sucrose at a lower concentration (20 g/L),and we used sucrose at 30 g/L as the control. Paclobutrazol (7 mg/L) significantly suppressed cell growth and produced the highest level of quinine (11%) after 7 weeks of culture. L-tryptophan also reduced cell growth, but without any positive effect in the production of quinoline. The highest amount of quinine per culture flask, however,  resulted in cells treated with 3 mg/L abscisic acid.
Identification of Secretory Structure, Histochemistry and Phytochemical Compounds of Medicinal Plant Hyptis sapitata Jacq. Rupa, Darius; Sulistyaningsih, Yohana Caecilia; Dorly, Dorly; Ratnadewi, Diah
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2017.24.2.499

Abstract

Hyptis capitata Jacq. (common name: Knobweed or False Ironwort) belongs to Lamiaceae family and is among known traditional medicine. The Anak Dalam Tribe of Jambi Province uses the leaves of H. capitata to cure external and internal wounds. This study was aimed at identifying and analyzing secretory structure, histochemistry and phytochemical content of H. capitata leaves.  The results showed that H. capitata leaves have secretory structures in the form of peltate, capitate and uniseriate glandular trichomes on the upper and lower leaf surfaces, with idioblast cells scattered throughout the leaf mesophyll. Histochemical tests indicated that the peltate trichomes have four head cells, containing alkaloids and terpenoids. This study classified capitate trichomes into two types: 1. type I has one stalk cell and two head cells, all containing  alkaloids, terpenoids and lipophilic compounds; 2. type II has long stalk cells consisting of seven to ten cells with one head cell, all containing alkaloids and terpenoids. Uniseriate glandular trichomes of H. capitata leaves consist of four to eight cells containing alkaloids and terpenoids. The idioblast cells are round-shaped and contain lipophilic compounds. GC-MS analysis showed that H. capitata leaves  contain terpenoid compounds assumed to serve as anti-infective agents, including l-limonene, eugenol, farnesol isomers A, d-nerolidol, hexahydrofarnesol and neophytadiene.
Co-Authors . Suharyo . SUMARYONO . TRIVADILA Adabiyah, Rifatul Ai Nurhasanah Husnul Izzati Amallia, Nur Anggraini Barlian Aris Tjahjoleksono Atik Choirul Hidajah C Hanny Wijaya Christofora Hanny Widjaja Dian Rahma Pratiwi Dorly Dorly Dyah Iswantini Dyah Retno Wulandari Dyah Retno Wulandari Edhi Sandra Efah FITRAMALA Elsa May Susanti ENDANG HADIPOENTYANTI Erwin Al Hafiizh Eva KHAERUNNISA Gustania, Leolita Hadi SUNARSO Hari Basuki Notobroto Hasibuan, Yustiny Andaliza Hayati MINARSIH Ika Dewi Ana Imron RIYADI Khalisa Aini Sinaga Khalisa Aini Sinaga Leksonowati, Aryani Lilis Solihat Mas’ud, Zainal Alim Maulidiyah Utami Muhamad Ayi Pandu Perdana Nina Ratna Djuita Nur Amallia Nurmuliayanti Muis Nurmuliayanti Muis Nur`Aeni, Fitri Rahmi, Putri Rifatul Adabiyah Rinny Saputri Rizka Musdalifah Amsar Rukmi, Paramita Sadha Dayinta Rupa, Darius RUSMILAH SUSENO Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirjo Soekisman Tjitrosoedirjo Sulistyaningsih, Yohana Caecilia Sumaryono . Titiek Setyawati Titiek Setyawati Titiek Setyawati Titiek Setyawati Tjitrosoedirdjo, Soekisman Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Triati Dewi Kencana Wungu Wijayanta, Ardian Nur WIRDHATUL MUSLIHATIN Witjaksono Witjaksono WOLFGANG FRANK Yohana Caecilia Sulistyaningsih Yustiny Andaliza Hasibuan Zainal Alim Mas’ud Zainal Alim Mas’ud