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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Agro Jurnal Sumberdaya Hayati Indonesian Journal of Science and Technology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Celebes Biodiversitas : Jurnal Sains dan Pendidikan Biologi Jurnal Biologi Tropis Menara Perkebunan Jurnal Jamu Indonesia EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Indonesian Journal of Jamu

Diah Ratnadewi

Biology Department, Fac. Mathematics And Natural Sciences, IPB University

Author-ID : 253482

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Variabilitas Genetik Berbagai Varietas Abaka (Musa Textilis Nee) dan Kerabat Liar Melalui Analisis RAPD Endang Hadipoentyanti; Diah Ratnadewi; Lilis Solihat
Zuriat Vol 12, No 2 (2001)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v12i2.6700

Abaka (Musa textilis Nee) merupakan tanaman penghasil serat yang digunakan dalam berbagai industri. Indonesia memiliki potensi dalam pengembangan tanaman tersebut. Dalam penelitian ini dipelajari variabilitas genetik 30 nomor tanaman abaka dan kerabat liarnya hasil eksplorasi dari daerah Bogor, Serang, Malang, Banyuwangi, dan Palu berdasarkan pola pita hasil random amplified polymorphic DNA (RAPD). Praimer yang digunakan dalam proses RAPD sebanyak lima buah, yaitu praimer abi 117.17, abi 117.18, OPB 18, OPC 15, dan OPD 08. Hasil RAPD dicatat berdasarkan ada atau tidaknya pita, dan dianalisis menggunakan program SIMQUAL-similarity for qualitative data yang ada pada Numerical Taxonomy and Multivariate Analysis System (NTSys) versi 1.80. Varians genetik ditentukan berdasarkan metode Unweight Pair Group Methode by Average (UPGMA). Pola pita DNA yang dihasilkan sebanyak 69 pola pita dengan ukuran antara 0.25 kb-3 kb. Jumlah pita DNA per nomor tanaman adalah 1 pita-9 pita. Rata-rata jumlah pita dari masing-masing tanaman sebanyak 4 pita. Dendogram menghasilkan dua kelompok tanaman, yakni kelompok A dan B dengan kesamaan genetic sekitar 47%. Kelompok A berjumlah 14 tanaman dan terbagi ke dalam 7 sub kelompok. Sedangkan kelompok B terdiri dari 16 tanaman dan terbagi ke dalam 5 sub kelompok. Tanaman nomor 1, 2, 3 dan 5 diperkirakan termasuk ke dalam spesies atau varietas yang sama, juga nomor 10, 16, dan 19, serta nomor 20 dengan 23.

Pertumbuhan Planlet Lidah Mertua (Sansevieria sp.) Blue Leaf dari Kultur Kalus Diah Ratnadewi; Ai Nurhasanah Husnul Izzati; Aris Tjahjoleksono
Jurnal Sumberdaya Hayati Vol. 1 No. 1 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.1.15-18

Lidah mertua (Sansevieria sp.) Blue Leaf is a slow growing ornamental plant. It is also difficult to reproduce. Therefore market demands for this plant is impossible to fulfill through conventional methods of propagation such as leaf cutting or layering. Tissue culture is one of the alternatives to solve the problem of plant propagation. In vitro culture of Sansevieria calli was induced for shooting, and rooting afterwards. The basic media of WP was superior than MS in producing multiple shoots, and this WP media containing of BAP 5 mg/L and NAA 0,5 mg/L (WH1) gave the highest number of shoots and leaves on plantlets, as well as the best growth performance. The shoots started to appear two weeks after culture. Plantlets from WH1 rooted in higher frequency as well on rooting media. A mixture of compost, sand, and zeolit (4:4:1) enriched with a nutritive solution, composting of 1⁄4 WP plus IBA 0.5 mg/L, was a poreous media that was able to induce rooting of this plant.

Kultur in vitro pisang (Musa paradisiaca L.) cv. Kepok Merah untuk mikropropagasi cepat [In vitro culture of banana (Musa paradisiaca) cv. Kepok Merah for rapid micropropagation] Efah FITRAMALA; Eva KHAERUNNISA; Nina Ratna Djuita Ratna DJUITA; Hadi SUNARSO; Diah RATNADEWI
E-Journal Menara Perkebunan Vol 84, No 2 (2016): Desember 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Banana (Musa paradisiaca L) cv. Kepok Merah has a high commercial value as it is used in food industries such as banana chip. Besides, Kepok Merah contains high B-complex vitamins that serve in energy metabolism and in the development of infant brain. The establishment of industrial plantations of this plant has been restricted by the lack of planting materials. This research aimed at ameliorating the capacity of plantlets multiplication up to rooting of this banana in a rapid way through in vitro multiplication techniques. Murashige and Skoog (MS) and Woody Plant (WP) media were used as the basic media. For the initiation stage, the media was fortified with 0.2 mg/L IAA and two levels of BA at 3 and 5 mg/L. For shoot multiplication, the concentrations of IAA as well as BA were increased. For rooting, 1 mg/L NAA or IBA was applied. The observations demonstrated that for shoots initiation, both basic media performed good results when enriched with 0.2 mg/L IAA and 5 mg/L BA. The highest rate of shoots multiplication at 6 – 17 shoots per explant, was obtained on MS medium added with 0.5 mg/L IAA and 5 mg/L BA. NAA at 1 mg/L in MS medium produced more rooted plantlets, 3 – 16 roots per plantlet, than those of other treatments. [Keywords: Musa paradisiaca cv. Kepok Merah, in vitro micropropagation, scalps.]AbstrakPisang (Musa paradisiaca L.) kultivar Kepok Merah memiliki nilai komersial yang cukup tinggi yaitu sebagai bahan dalam industri pembuatan keripik pisang. Selain itu, pisang Kepok Merah memiliki kandungan vitamin B kompleks cukup tinggi untuk membantu produksi energi dan pembentukan sel-sel otak pada bayi. Pertanaman pisang ini dalam skala industri terkendala oleh kurangnya ketersediaan sumber benih. Teknik kultur jaringan diharapkan dapat menghasilkan benih secara massal dalam waktu yang relatif singkat. Tujuan dari penelitian ini adalah meningkatkan keberhasilan multiplikasi tunas in vitro hingga pengakaran tanaman pisang Kepok Merah secara cepat. Pada tahap inisiasi tunas digunakan media dasar Murashige and Skoog (MS) dan Woody Plant (WP), ke dalam media dasar tersebut ditambahkan IAA 0,2 mg/L dan 2 taraf BA yaitu 3 dan 5 mg/L. Multiplikasi tunas dilakukan pada media dasar yang sama namun dengan taraf konsentrasi IAA serta BA yang ditingkatkan. Tahap perakaran menggunakan media dasar MS dan WP dengan auksin NAA 1 mg/L atau IBA 1 mg/L. Hasil penelitian menunjukkan bahwa untuk inisiasi tunas, media MS dan WP yang diperkaya dengan IAA 0,2 mg/L dan BA 5 mg/L sama baiknya. Untuk multiplikasi tunas, media MS dengan IAA 0,5 mg/L yang dikombinasikan dengan BA 5 mg/L memberikan jumlah tunas paling banyak, yaitu 6 – 17 tunas per eksplan, dan pertumbuhannyapun lebih baik. Pemberian NAA 1 mg/L pada media MS dapat memberikan lebih banyak tunas yang berakar, dengan jumlah akar 3 – 16 per planlet. [Kata kunci: Musa paradisiaca cv. Kepok Merah, mikropropagasi in vitro, nodul meristematik.]

Pengaruh jumlah subkultur dan media sub-optimal terhadap pertumbuhan dan kemampuan regenerasi kalus tebu (Saccharum officinarum L.) (Effect of repeated subculture and suboptimum media on the growth of sugarcane calli (Saccharum officinarum L.)) Hayati MINARSIH; . Suharyo; Imron RIYADI; Diah RATNADEWI
E-Journal Menara Perkebunan Vol 84, No 1: Oktober 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Sugarcane (Saccharum officinarum L.) is an important crop for sugar production. One attempt to increase sugarcane productivity is through micropropagation and quality improvement of sugarcane seedlings in vitro. This research aimed to study the effect of repeated subcultures on callus capacity for regeneration and plant survival in acclimatization phase, as well as the influence of suboptimum media on the recovery capability of sugarcane callus to proliferate in vitro. Fourth subcultured sugarcane callus derived from young leaves were used as material in this research. Basic medium of Murashige and Skoog (MS) added with 3 mg/L 2,4-D, 10% coconut water, and 3% sucrose was used for callus initiation. For callus regeneration, the MS medium was supplemented with 2 mg/L BAP, 0.2 mg/L IAA, 10% coconut water, and 3% sucrose. Study on the effect of subculture numbers consisted of three stages, i.e. initiation, regeneration, and acclimatization, while the study on resting phase or the use of sub-optimal media included six treatment media and two pathways. Results showed that the fifth subcultures produced embryoid callus (91%), the highest non mucilaginous callus (97%), and the highest abnormality rate (6%). Results from the suboptimum media treatment, showed that B pathway (4 week resting phase) was better than the A pathway (8 week resting phase), based on fresh weight and callus abnormality percentage. A and B pathways indicated that the growth of callus can be recovered when it was grown back to the normal media and 1.5D-MS treatment of the resting phase showed the best growth and appearance.

Production of Secondary Metabolite Compounds of Gotu Kola (Centella asiatica) Under Salinity and Drought Stress Nur Amallia; Zainal Alim Mas’ud; Diah Ratnadewi
Jurnal Jamu Indonesia Vol. 5 No. 2 (2020): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Gotu kola (Centella asiatica) is a traditional herbal plant that has been reported have a variety of pharmacological activities. The compounds of gotu kola that play a role on pharmacological activities are triterpene group compounds, namely madecasosside (MD), asiaticoside (AS), madecassic acid (AM), and asiatic acid (AA). Stress can affect the production of biomass and secondary metabolite compounds in plants. The aims of the study is to analyze the effect of salinity and drought stress on biomass and 4 compounds of triterpene in gotu kola. Harvesting is done when the plant is 8 weeks old. The yield of the biomass was analyzed and then extracted using methanol solvent to be analyzed secondary metabolite levels using the high performance liquid chromatography (HPLC) method. The lowest crop biomass was obtained at 50% of field capacity and 3,000 ppm salt content. The highest levels of MD and AS were established in conditions of 100% field capacity and 1,000 ppm salt content. The 50 and 100% field capacity and 1,000-3,000 ppm salt content did not affect the levels of AM and AA.

Isolation of Native Plant-Derived Exosome-like Nanoparticles and Their Uptake by Human Cells Diah Ratnadewi; Christofora Hanny Widjaja; Anggraini Barlian; Rizka Musdalifah Amsar; Ika Dewi Ana; Atik Choirul Hidajah; Hari Basuki Notobroto; Triati Dewi Kencana Wungu
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.182-192

The exosome is a nano-sized vesicular particle commonly secreted from eukaryotic cells to extracellular space. Exosome functions in cellular communication, nutrients or bioactive compounds delivery, and cellular immunity. It is believed that plant-derived exosome-like particle (PDEN) potential to deliver nano-bioactive compounds to the human body. As Indonesia is one of the herbal centers of the world, we are encouraged to develop natural richness for human welfare. The study aimed to verify various methods to isolate exosomes from the fresh ginger rhizome and quina cells and examine the ginger PDENs' capacity to internalize in vitro human cells. The PDENs isolation was carried out by centrifugation and precipitation. The particles were observed through a transmission electron microscope, dynamic light scattering, and particle size analysis. Differential ultracentrifugation combined with PEG6000 provided a better size range of particles. Nanoparticles tracking analysis performed the concentration of particles within a specific size range. Ginger and quina PDENs size ranged from 40-650 nm, and the concentration was 3.6 × 107 and 2.8 × 106, respectively. The PKH67-labelled ginger PDENs were taken up by human dermal fibroblast and human Wharton's jelly mesenchymal stem cells after 30 minutes and 21 hours. DPPH assays revealed that ginger PDENs have antioxidant activity, indicating the presence of some nano-molecules in those particles. This study would be the baseline for developing ginger PDENs as a functional food with nano-ingredients, or as an effective vehicle of nano-drug, for human health.

Optimasi komposisi media untuk mikropropagasi tanaman kupa (Syzygium polycephalum (Miq.) Merr. & L.M Perry) Muhamad Ayi Pandu Perdana; Diah Ratnadewi; Tri Muji Ermayanti
Jurnal Agro Vol 9, No 2 (2022)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/20958

Kupa (Syzygium polycephalum (Miq.) Merr. & L.M Perry) merupakan tanaman berkayu yang dapat dijadikan sebagai bahan obat dan zat pewarna. Kupa sudah jarang ditemukan di beberapa wilayah Indonesia, oleh karena itu diperlukan upaya konservasi. Kultur jaringan adalah salah satu teknik yang dapat diaplikasikan untuk perbanyakan tanaman dan konservasi. Penelitian ini bertujuan untuk mendapatkan komposisi media yang optimum untuk mikropropagasi kupa. Penelitian terdiri atas 3 tahap percobaan, yaitu multiplikasi tunas menggunakan eksplan buku tunggal dan tunas pucuk pada media dasar DKW dan MS, yang mengandung BAP dan IBA; perakaran menggunakan substrat agar atau campuran pasir + vermikulit, ditambah larutan DKW atau MS, dan IBA; dan aklimatisasi. Semua percobaan menggunakan Rancangan Acak Lengkap Faktorial. Hasil penelitian menunjukkan bahwa media MS + BAP 1 mg L-1 + IBA 0,1 mg L-1 merupakan media terbaik untuk perbanyakan dan tinggi tunas dari eksplan buku tunggal. Tunas terbanyak dari eksplan tunas pucuk didapatkan dari media DKW + BAP 1 mg L-1 + IBA 0,1 mg L-1. Media Pasir + Vermikulit dengan larutan MS maupun DKW memberikan 100% tunas berakar. Planlet yang berasal dari media pasir + vermikulit dengan pemberian larutan MS menghasilkan jumlah dan panjang akar lebih baik, dan daya hidup tertinggi pada tahap aklimatisasi.ABSTRACTKupa (Syzygium polycephalum (Miq.) Merr. & L.M Perry) is a woody plant, which has many benefits including herbal medicine and coloring matter. Kupa is rarely found in some parts of Indonesia, therefore the conservation is needed. Tissue culture is a technique applied for propagation and conservation. This study aimed to obtain the optimal media composition for kupa micropropagation. The study consisted of 3 steps, i.e. shoot multiplication using single node and shoot tips explants cultured in basic media DKW and MS containing BAP and IBA; rooting in two different substrates, agar and a mixture of vermiculite + sand, enriched with DKW or MS solution, and IBA; and acclimatization. All experiments used a factorial completely randomized design. The results showed that MS +BAP 1 mg L-1 + IBA 0.1 mg L-1 was the best for shoot multiplication and shoot height from single node explants. Media of DKW + BAP 1 mg L-1+ IBA 0.1 mg L-1 was the best for multi-shoots from shoot tip explant. Sand + vermiculite media with MS or DKW solution produced 100% rooted-plantlets. Plantlets derived from sand + vermiculite with MS solution gave the best root number and length, and it also the highest survival rate at the acclimatization stage.

Morphological and Anatomical Comparison between Tetraploid Stevia rebaudiana (Bertoni) Bertoni and its Parental Diploid Rifatul Adabiyah; Diah Ratnadewi; Tri Muji Ermayanti; Erwin Al Hafiizh; Elsa May Susanti
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.321-335

Stevia rebaudiana (Bertoni) Bertoni is a perennial herbaceous plant that produces natural low glycemic-index sweeteners alternative to sugar cane. The genetic improvement of Stevia needs to be investigated to increase its productivity. Although this plant has a narrow genetic diversity, genetic improvement through polyploidization may succeed. Meanwhile, genotypic characters of tetraploid Stevia were previously reported. This study then observed the phenotypic characters of the tetraploid plants, which aimed to evaluate the morphological and anatomical characteristics of tetraploid Stevia clones compared to its wild-type parental diploid plant. Three tetraploids and one diploid clone were grown in the field for 12 weeks. The results of qualitative descriptive characters showed that the tetraploid clones B60.3H8, P1T22 and P3T5 had habitus type, shoot bud shape, leaf margin, leaf venation, shape, and type of stem, type of flower, and seed similar to the diploid parental clone. However, the quantitative characters showed that the tetraploid clones had leaf size, stem diameter, root size, length of the single flower, length of the flower stalk, and length of fruit which were more extensive than those of the parental diploid clone. The leaf color of tetraploids was a darker green, and the range of initial flowering times was earlier. This finding gives more understanding of the phenotypic and anatomical characteristics of Stevia tetraploids compared to their parental diploid plant.

Alkaloids Production and Cell Growth of Cinchona ledgeriana Moens: Effects of Fungal Filtrate and Methyl Jasmonate Elicitors Yustiny Andaliza Hasibuan; Diah Ratnadewi; Zainal Alim Mas’ud
Indonesian Journal of Science and Technology Vol 6, No 1 (2021): IJOST: VOLUME 6, ISSUE 1, April 2021
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/ijost.v6i1.31479

Cinchona alkaloids are known as antimalaria and anti-arrhythmic. Due to the long waiting time to harvest, cell culture technology is a challenge. This study aimed to determine the effects of elicitors, filtrate of two strains of endophytic fungi and methyl jasmonate (MeJA), in cell suspension culture of Cinchona ledgeriana on quinine and quinidine production. The cells were cultured for seven weeks in woody plant (WP) media treated with either of those elicitors in various concentrations. The cells growth was observed and the alkaloids were analyzed by HPLC. Cells treated with MeJA failed to grow that led to the cell biomass insufficiency for alkaloids determination. It indicates that the cells are quite sensitive to even low concentration of MeJA that hampered the growth. Cells treated with the filtrate of Diaporthe sp. M13-Millipore filtered (S2M) gave the least cell biomass but presented the highest content of both alkaloids. Diaporthe sp. strain M-13 is stronger as elicitor than M-23 for this plant species. Filtrate of non-virulent fungi can elevate the biosynthesis of alkaloids. This reconfirms that cultured cells are capable to produce secondary metabolites and the productivity can be increased by using an appropriate elicitor.

Efektifitas Triklopir dan Fluroksipir dalam Pengendalian Gulma Berdaun Lebar di Savana Bekol Taman Nasional Baluran Rinny Saputri; YM Diah Ratnadewi; Soekisman Tjitrosoedirdjo; Titiek Setyawati
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 7 No 1 (2022): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Invasion of broadleved weeds cause competition with local grasses which are the main feed for herbivores. Chemical control should use selective herbicides that only kill the broadleaved weeds without harming the grass. The research was conducted in the savanna Bekol - Baluran National Park, East Java, from January 2015 until March 2015 to find the best rate of herbicide to control broadleaved weeds in the savanna and to estimate the soil residue of herbicide used. The experimental design was a randomized block with six treatments and four replications. The treatments were herbicide applications consisted of (1) the rate of triclopyr at 670 g a.i ha-1 (TA), (2) rate of triclopyr at 1340 a.i ha-1 (TB), (3) the rate of fluroxypyr at 200 g a.i ha-1 (FA), (4) rate of fluroxypir at 400 g a.i ha-1 (FB), (5) Weeding Manual (PM) and (6) Control (K).The result showed that there was a change in the composition of the vegetation after herbicide applied. The dominance of broadleaed weeds was replaced by the dominance of Brachiaria reptans and Sclerechnae punctata. The application of triclopyr at 1340 g ai ha-1 was able to reduce weed infestation to 0.48 g 0.25 m-2 compared to 12.66 g 0.25 m-2 before the herbicide application. It facilitated the growth of grasses up to 6.87 g 0.25 m-2 which was among the best of treatments applied because it has shown maximum effectieness for controlling broadleaved weeds.

Co-Authors . Suharyo . SUMARYONO . TRIVADILA Adabiyah, Rifatul Ai Nurhasanah Husnul Izzati Amallia, Nur Anggraini Barlian Aris Tjahjoleksono Atik Choirul Hidajah C Hanny Wijaya Christofora Hanny Widjaja Dian Rahma Pratiwi Dorly Dorly Dyah Iswantini Dyah Retno Wulandari Dyah Retno Wulandari Edhi Sandra Efah FITRAMALA Elsa May Susanti ENDANG HADIPOENTYANTI Erwin Al Hafiizh Eva KHAERUNNISA Gustania, Leolita Hadi SUNARSO Hari Basuki Notobroto Hasibuan, Yustiny Andaliza Hayati MINARSIH Ika Dewi Ana Imron RIYADI Khalisa Aini Sinaga Khalisa Aini Sinaga Leksonowati, Aryani Lilis Solihat Mas’ud, Zainal Alim Maulidiyah Utami Muhamad Ayi Pandu Perdana Nina Ratna Djuita Nur Amallia Nurmuliayanti Muis Nurmuliayanti Muis Nur`Aeni, Fitri Rahmi, Putri Rifatul Adabiyah Rinny Saputri Rizka Musdalifah Amsar Rukmi, Paramita Sadha Dayinta Rupa, Darius RUSMILAH SUSENO Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirjo Soekisman Tjitrosoedirjo Sulistyaningsih, Yohana Caecilia Sumaryono . Titiek Setyawati Titiek Setyawati Titiek Setyawati Titiek Setyawati Tjitrosoedirdjo, Soekisman Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Triati Dewi Kencana Wungu Wijayanta, Ardian Nur WIRDHATUL MUSLIHATIN Witjaksono Witjaksono WOLFGANG FRANK Yohana Caecilia Sulistyaningsih Yustiny Andaliza Hasibuan Zainal Alim Mas’ud Zainal Alim Mas’ud

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