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Effect of Ethanolic Extract of Avocado (Persea americana Mill.) Seed on the Testis Histology (Mus musculus l.) Induced Monosodium Glutamate (MSG) Yusni Atifah; Dwi Junita Zega; Yuni Ahda; Siska Alicia Farma
BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Vol 12, No 2 (2024): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v12i2.24419

Abstract

Monosodium Glutamate (MSG) is a substance that is able to produce free radicals such as ROS (Reactive Oxygen Species), which can cause oxidative stress in the body and damage reproductive organs such as the testes. The purpose of this study was to determine the effect of avocado seed ethanol extract (Persea americana Mill.) on the histology of the testes of mice (Mus musculus L.) induced monosodium glutamate (MSG). This research is an experimental study that was conducted in October 2023 – January 2024 at Biology Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang. The design used is a Complete Randomized Design (CRD) with 4 treatments and 6 repeats. The data obtained were analyzed using the ANOVA test (Analysis of variance) using the SPSS 23 application. The results showed that the administration of avocado seed ethanol extract showed a noticeable effect on the diameter of the testes of male mice exposed to MSG. However, it showed no noticeable influence on testicular weight, seminiferous tubule diameter and Leydig cell count. Ethanol extract avocado seed dose 0.84 mg /g body weight / day (P1) was able to improve the diameter of mice testes that had been induced MSGKeyword: Avocado seed extract, Histology of testis, Mice, MSG
Penyakit Hirschsprung di Indonesia: Faktor Potensial yang Berkontribusi dan Analisis Silsilah Yuni Ahda; Khairunnisa; Zuhrah Taufiqa; Diana Lyrawati; Risa Ukhti Muslima
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9301

Abstract

Hirschsprung’s disease (HD) has a complex origin involving genetic factors. While HD cases have been documented in Indonesia, the genetic inheritance is unexplored. This study aims to update HD in Indonesia, determine the inheritance pattern through pedigree analysis, and identify the non-genetic factors contributing to HD, including parental demographics and maternal aspects. A cross-sectional survey was conducted with 87 respondents across Indonesia. The results revealed a male-to-female incidence ratio of 1.90:1, with the ultra-short segment being the most common type (75.86%), and familial cases accounted for 8%. Seventeen patients exhibited other congenital abnormalities, with pneumonia, redundant colon, and cardiac defect as the highest cases. Over 70% of the parents were aged 20-35 and had relatively high education and income levels. Notably, nearly half of the mothers experienced food aversion in the first trimester (n=38) and frequently consumed fast or instant food during pregnancy (n=33). The study confirmed that HD follows an autosomal recessive inheritance pattern. These findings emphasize the role of genetic inheritance in incidents of HD, which is valuable for developing early detection strategies and providing genetic counseling for at-risk families. Maternal lifestyle factors may play a role in the development of HD, which needs further investigation.
The Impact of Problem-Based Learning on Science Learning to Improve Students' Critical Thinking and Problem-Solving Skills: A Meta-Analysis Nurul Aripin; Festiyed; Yerimadesi; Heffi Alberida; Yuni Ahda; Fatni Mufit
Jurnal Penelitian Pendidikan IPA Vol 11 No 6 (2025): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i6.10051

Abstract

This meta-analysis investigates the effectiveness of the Problem-Based Learning (PBL) model in enhancing students' critical thinking and problem-solving skills in science education. A total of 42 peer-reviewed articles published between 2020 and 2024 were analyzed, covering diverse educational levels and scientific disciplines. The analysis revealed a large average effect size (ES = 0.798; 95% CI: 0.782–0.813), indicating a strong positive impact of PBL. The low standard error (SE = 0.043–0.060) reflects high estimation precision. Statistical tests, including funnel plot symmetry, Trim-and-Fill analysis (p = 0.991), and Egger’s regression test (p = 0.947), confirm the absence of significant publication bias. Moreover, residual heterogeneity was minimal (τ² = 0.000), validating the appropriateness of the fixed-effects model. These findings provide robust empirical evidence that PBL is consistently effective across various contexts in improving students’ 21st-century competencies. The study offers meaningful implications for educators and policymakers in designing science learning strategies that foster higher-order thinking skills.
The Development of Inquiry-Based LKPD to Enhance Critical Thinking Skills in Science learning at Elementary Schools Sisri Wahyuni; Yeni Nurpatri; Festiyed; Yerimadesi; Heffi Alberida; Yuni Ahda
Jurnal Penelitian Pendidikan IPA Vol 11 No 3 (2025): March
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i3.10157

Abstract

This research is motivated by the development of LKPD (Student Worksheet) for enhancing critical thinking skills of elementary school students. The LKPD used has not yet captured the students' attention. The LKPD has not been able to improve students' learning motivation because it does not align with their learning styles. LKPD serves as teaching material used by teachers to facilitate the delivery of content and achieve learning objectives. This study aims to develop LKPD and assess its validity using the Inquiry model to improve critical thinking skills in Science education about the conservation of natural resources in the 4th-grade class. The type of research used is research and development (R&D), employing the 4-D model. This model consists of four development stages: define, design, development, and dissemination. Based on the results of the validation of LKPD using the Inquiry model to enhance critical thinking skills, the LKPD is deemed feasible for use. The material aspect validation result was 85.41% (valid), the design aspect validation result was 86.53% (valid), and the language aspect validation result was 94.44% (very valid). Therefore, the LKPD using the Inquiry model to improve critical thinking skills is valid.
Development of a Problem-Based Instruction Student Worksheet on Environmental Change to Improve Senior High School Students’ Scientific Literacy Threesia Anugrah Ilahi; Irdawati; Yuni Ahda; Fitri Arsih; Suci Fajrina
Al Jahiz Vol 6 No 2 (2025): Al-Jahiz: Journal of Biology Education Research, July-December 2025
Publisher : Faculty of Tarbiyah and Teacher Training UIN Jurai Siwo, Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32332/al-jahiz.v6i2.11159

Abstract

This research aims to develop PBI-based student worksheet to improve science literacy and critical thinking of high school grade X students. The research method uses the Plomp Research and Development (R&D) model which consists of initial investigation, design, and evaluation stages. Formative evaluation refers to the Tessmer model, including self-evaluation, expert review, one-to-one, small group, and field test. The instruments used included validation sheets, response questionnaires, and science literacy ability tests. The subjects of the study were 29 students divided into experimental and control classes. The validation results showed that student worksheet was very valid (average score of 87.5%), practical (student and teacher response ≥85%), and effective in improving science literacy. The increase in science literacy gain score was 0.48 (experimental class) and 0.36 (control). The t-test showed significant differences between groups (p < 0.05). Thus, PBI-based student worksheet is suitable for use as a learning medium to improve students' 21st century skills.
Pengaruh Model Pembelajaran Kooperatif Two Stay Two Stray (TSTS) Berbantuan Media Interaktif Berbasis STEM Terhadap Berpikir Kritis dan Hasil Belajar Siswa Pada Materi Sistem Indera Di SMA Negeri 2 Kerinci Eria Marina Sepriyani; Lufri; Irdawati; Yuni Ahda
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4551

Abstract

This study aims to determine the effect of the two-stay two stray cooperative learning model (TSTS) assisted by STEM-based interactive media on critical thinking and student learning outcomes on sensory system material at SMA Negeri 2 Kerinci. This type of research is quasi-experimental research with a randomized control-group posttest-only design. The population of this study came from class XI students of SMA Negeri 2 Kerinci for the 2021/2022 Academic Year. The sampling technique used is a purposive sampling technique, namely that the determination of the sample takes into account certain criteria that have been made for the subject according to the research objectives. Data analysis techniques used the normality test, homogeneity test, and hypothesis test. Based on the research that has been done, it can be concluded that the STEM-based two stay two stray learning model has an effect on critical thinking and student learning outcomes on sensory system material at SMA Negeri 2 Kerinci.
Effectiveness of Higher Order Thinking Skills-based Test Instruments in Science Learning in Indonesia: A Meta-analysis Tomi Apra Santosa; Siska Angreni; Rona Taula Sari; Festiyed; Yerimadesi; Yuni Ahda; Heffi Alberida; Fitri Arsih
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.6654

Abstract

This research is motivated by the many applications of Higher Order Thinking Skills-based test instruments in science learning. However, it has not been found how effective the instrument is in science learning. The purpose of this study was to determine the effectiveness of Higher Order Thinking Skills-based test instruments in science learning. This type of research is a meta-analysis. Data sources come from the databases of Google Scholar, Hindawi, ScienceDirect, Wiley, Taylor of Francis, Sage Journal, Springer, ProQuest and ERIC. Search keywords are test instruments, test instruments based on Higher Order Thinking Skills in science learning, and higher order thinking skills. This study analyzed 20 articles published in 2020-2023 used in the meta-analysis. Data analysis techniques with the help of JSAP software. The results concluded that the summary effect size value was 1.24 (very high effect size). This finding shows that the use of test instruments based on Higher Order Thinking Skills is effectively applied in science learning. Furthermore, the research provides higher order tinking skills-based test instruments in science learning in Indonesia and provides important information for the application of higher order thinking skills-based test instruments in the future
Development Research in Science Education: A Systematic Literature Review of Trends in Development Models and Instruments Used Ratih Komala Dewi; Darmanella Dian Eka Wati; Oria Lasmana; Yerimadesi; Festiyed; Yuni Ahda; Heffi Alberida
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.6876

Abstract

This study was motivated by the desire to know the trend of development models and instruments used in development research.  The purpose of this study was to conduct a comprehensive review of the existing literature on development research in science education. The focus was to identify trends in development models and instruments used in this field. This research is a type of systematic literature review using the PRISMA (Preferred Reporting Items for Systematic reviews and Meta-Analyses) method to look at trends in development models and instruments in science education development research. This research uses Scopus, Science Direct and Eric databases. The results of this study are the most widely used development model is the ADDIE development model and questionnaires are the instruments used in all articles analyzed.
Pengembangan E-Modul Berbasis Android Berbentuk Flipbook pada Materi Sistem Ekskresi dan Sistem Koordinasi Untuk Peserta Didik Kelas XI SMA Nanta Mulia; Linda Advinda; Syamsurizal; Yuni Ahda
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.7237

Abstract

Increasingly developing technology encourages educators to use it in the learning process as a learning medium in order to increase student’s interest in learning. However, currently there are still many who do not optimally use technology-based learning media. The learning media used is monotonous and unable to visualize the material on the excretion system and coordination system in humans. This has an impact on student learning outcomes which are still below the school's Minimum Completion Criteria. Therefore, technology-based learning media such as e-modules in the form of flipbooks are needed. This research aims to produce an Android-based e-module in the form of a flipbook on excretion system and coordination system material that is valid, practical and effective. The type of research used is development research using the Plomp development model which consists of three stages, namely the initial investigation stage, the development stage (prototyping), and the assessment stage. The instruments used were teacher interview guide instrument sheets, student questionnaire sheets, self-evaluation sheets, and e-module validation sheets by experts, as well as e-module practicality assessment sheets in the form of flipbooks filled in by teachers and students. The subjects of this research were class XI high school students. The research object is an Android based e-module in the form of a flipbook on excretion system and coordination system material. The research results show that the Android based e-module in the form of a flipbook obtained very valid criteria validity results with a value of 86.76% and 89.06%, practicality results by teachers and students with very practical criteria with a value of 84.17% and 86.43%. Based on the T test carried out, there is a difference in value between the experimental class and the control class, namely a significance of 0.048 in the excretion system material and 0.003 in the coordination system material. Thus, Android-based e-modules in the form of flipbooks can improve students learning competence.
Isolasi DNA Jamur dari Tumbuhan Alpukat dengan Metode Spin Column Alya Nabila; Yuni Ahda
Jurnal Sains Dan Teknologi | E-ISSN : 3063-9980 Vol. 2 No. 4 (2026): April - Juni
Publisher : GLOBAL SCIENTS PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The advancement of molecular biology requires efficient nucleic acid extraction methods to overcome the complexity of biological samples. Fungi associated with avocado plants possess strong cell walls and the presence of host secondary metabolites that may potentially inhibit molecular analyses. This study aimed to isolate and evaluate the quantity and quality of fungal DNA from avocado plants using a combination of mechanical disruption (bead beating) and spin column-based purification. Fungal DNA was isolated using the Quick-DNA™ Fungal/Bacterial Miniprep Kit. Quantification using a NanoDrop spectrophotometer showed that DNA concentrations ranged from 1.7 to 139.1 ng/µL. Most samples yielded DNA concentrations above 50 ng/µL with A260/280 purity ratios ranging from 1.87 to 1.98. Mechanical disruption using beads in a lysis tube facilitated the breakdown of fungal cell walls, while the silica column effectively purified DNA and reduced contaminants that could potentially interfere with analysis. Therefore, the combination of bead beating and spin column methods effectively produced high-quality genomic DNA suitable for further molecular analyses, such as Polymerase Chain Reaction (PCR).