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All Journal Bumi Lestari ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Pendidikan Sains SAINSTIS Journal of Education and Learning (EduLearn) Waste Technology Jurnal Pendidikan Biologi Indonesia MAJALAH BIAM Omni-Akuatika Pendas : Jurnah Ilmiah Pendidikan Dasar Biosaintifika: Journal of Biology & Biology Education Biopendix: Jurnal Biologi, Pendidikan dan Terapan BIOEDUPAT: Pattimura Journal of Biology and Learning SRIWIJAYA JOURNAL OF ENVIRONMENT Rumphius Pattimura Biological Journal Kognisi : Jurnal Penelitian Pendidikan Sekolah Dasar Sistem-Among : Jurnal Pendidikan Sekolah Dasar Biosaintifika: Journal of Biology & Biology Education Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Dominggus Rumahlatu
Study Program Of Biology Education, Faculty Of Teacher Training And Education, Universitas Pattimura, Jl. Ir. M. Putuhena, Ambon, Maluku 97233
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Mathematics Public Health Social Sciences Other

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Spatial and Seasonal Distribution of Cadmium and Lead in Sediment, Water and Its Response of Metal Transcription Factor-1 in Cardinal Fish Apogon beauforti Dominggus Rumahlatu; Estevanus Kristian Huliselan; Sriyanti Imelda A Salmanu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 1 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.958 KB) | DOI: 10.14710/ik.ijms.23.1.45-54

Abstract

Cadmium (Cd) and lead (Pb) are known to have high toxic effects and have been found polluting marine environment. Regular monitor the concentration of heavy metals in marine waters is necessary by combining chemical and biological analysis.  This research was conducted to analyze the levels of heavy metals in sediment, water column, cardinal fish Apogon beauforti, and to measure the concentration of MTF-1 protein in A. beauforti. This research was conducted at four sampling stations at Ambon bay for 2 years. The levels of Cd and Pb metals were analyzed using AAS instruments (Atomic Absorbsion Spectrophotometer), while the concentration of MTF-1 protein in the body of A. beauforti was analyzed using indirect ELISA. The results showed that the seasonal and spatial distribution of heavy metals Cd and Pb at Ambon bay did not show any variation. The results of the ANOVA proved that the concentrations of heavy metals Cd and Pb in the four research stations were not significantly different in the year 2013 and in the year 2014 in the samples of sediment, the water column, and the body of A. beauforti. In addition, it was revealed that the concentration of MTF-1 protein of A. beauforti in 2014 was higher than that in 2013, with an average of the concentration of MTF-1 protein of A.beauforti was higher at station 2, which was at Ferry Galala Harbor. Keywords: distribution, spatial, seasonal, cadmium, lead, MTF-1, Apogon beauforti
THE USE OF MOLECULAR MARKERS METALOTHIONEIN PROTEIN FOR MONITORING MARINE POLLUTION DUE TO HEAVY METALS MERCURY IN Apogon beauforti IN AMBON BAY Dominggus Rumahlatu; Fredy Leiwakabessy
Waste Technology Vol 4, No 2 (2016)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1998.848 KB) | DOI: 10.14710/4.2.9-14

Abstract

The heavy metal Hg is a global issue because it is toxic to living organisms. Therefore, monitoring levels of Hg in Ambon Bay is important to do. This is a study about monitoring the levels of heavy metals Hg in the waters of Ambon Bay in 2013 and 2014 by using Apogon beauforti as bio-indicators and Metalothionein-1 (MT-1) as a biomarker to determine the accumulation of Hg in relation to the concentration and expression of MT-1 in the tissue of A. beauforti. The samples used were 50 fish of A. beauforti taken at four different stations with purposive sampling procedure. The analysis of Hg content used AAS method, the expression of MT-1 was determined by western blotting method, and MT-1 concentrations were determined by using ELISA method in the laboratory of Brawijaya University. On the average, the results of the analysis of Hg content indicated that the accumulation of heavy metal Hg varied in four different stations. The levels of Hg in the tissue of A. beauforti in 2014 at station 1 increased, while the levels of Hg at stations 2, 3, and 4 decreased. However, the calculation results of MT-1 concentration by ELISA showed an increase in the concentration of MT-1 in 2014 compared to that in 2013. This result was supported with the fact that the protein tape expressed on the test results of western blotting was very thick. It indicated that the longer the organism exposed to heavy metals Hg, the more increased the accumulation of Hg. Thus, the expression and the concentration of MT-1 would also increase.
The Effects of Heavy Metal Cadmium Against Apoptosis Through Activation of Caspase-3 protein on Sea Urchins Diadema setosum Dominggus Rumahlatu
Jurnal Pendidikan Sains Vol 1, No 1: Maret 2013
Publisher : Pascasarjana Universitas Negeri Malang (UM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.352 KB) | DOI: 10.17977/jps.v1i1.3970

Abstract

Efek Logam Berat Kadmium Terhadap Apoptosis Melalui Aktivasi Protein Caspase-3 pada Bulu Babi Diadema setosum Abstract: This study is the Cd metal effect on apoptosis in cells D. setosum urchins. The study iss conducted in the laboratory of LIPI Ambon (Indonesia).  TheDoses of the treatment is dissolved 0.0, 1.0, 3.0, 6.0, 9.0, and 12.0 mg / L Cd.  Each treatment was repeated 7 times. The age of animal is about 8 months weighs 90 grams with a circumference of 15 cm body. The Experimental animals is fed with grass. The measurement of caspase-3 protein concentrations performed on the liver organ with method Caspase Colorimetric Assay Kit followed by staining Hematoxilen-eosin (HE). Data are analyzed with ANOVA test single and advanced to the MDRS 0:05. The results shows that Cd treatment significantly increases levels of Caspase-3 protein. Caspase-3 protein concentrations highest in treatment dose 12 mcg / L.  It is required for further studies which related to the potential increase in Caspase-3 protein as an alternative biomonitoring marine pollution by metals Cd. Key Words: Cadmium, Diadema setosum, Apoptosis, Caspase-3 Abstrak: Telah dilakukan penelitian mengenai efek logam Cd terhadap apoptosis pada sel bulu babi D. setosum. Penelitian dilakukan di laboratorium Balai LIPI Ambon (Indonesia). Dosis-dosis perlakuan adalah 0.0, 1.0, 3.0, 6.0, 9.0, dan 12.0 µg/L Cd terlarut. Pada tiap perlakuan diulang 7 kali. Usia hewan coba sekitar 8 bulan berbobot 90 gram dengan lingkar tubuh 15 cm. Hewan coba diberi makan lamun. Pengukuran konsentrasi protein caspase-3 dilakukan pada organ hepar dengan metode Caspase Colorimetric Assay Kit dilanjutkan dengan pewarnaan Hematoxilen-Eosin (HE). Data penelitian di-analisis dengan ANAVA tunggal dan uji lanjut dengan MDRS 0.05. Hasil penelitian memperlihatkan bahwa perlakuan Cd sangat signifikan meningkatkan kadar protein Caspase-3. Konsentrasi protein Caspase-3 tertinggi pada dosis perlakuan 12 µg/L. Diperlukan kajian lanjutan terkait potensi peningkat-an protein Caspase-3 sebagai satu alternatif biomonitoring pencemaran perairan laut oleh logam Cd. Kata kunci: Cadmium, Diadema setosum, Apoptosis, Caspase-3
STUDI TENTANG KEPADATAN, KELIMPAHAN DAN POLA PENYEBAR IKAN LAYUR (Trichiurus lepturus L) PADA PERAIRAN DESA PASSO TELUK BAGUALA AMBON Pieter Siahaya; Dominggus Rumahlatu
BIOPENDIX Vol 2 No 1 (2015): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol2issue1page33-37

Abstract

Background: Fish layur which is often called the people of Maluku as Fish Parang-parang is included order Perciformes in the Trchiuridae family and genus Trichiurus. The existence of fish in the waters Layur Passo not known with certainty. Methods: The study took place in October 2014. The study was divided into three locations of the research station. The method used in this study are visual census to calculate directly the large number of fish caught at the sites. Then the data were analyzed descriptively. Calculations used in the processing of the data using the formula of density, abundance, and distribution patterns. Environmental characteristics in measuring temperature, salinity, pH, and dissolved oxygen (DO). Results: The results showed that the water environmental condition Passo village is quite good for the survival of the organism. Conclusion: The density, abundance, and distribution pattern of fish layur relatively abundant in these waters with the highest value contained at two stations at the sites.
PENGARUH LAMA FERMENTASI TERHADAP CITA RASA NATA de Salacca Junita Salelatu; Dominggus Rumahlatu
BIOPENDIX Vol 3 No 1 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue1page46-52

Abstract

Background: Salak is one of Maluku's endemic fruits, especially in Buria Village which makes it the main outcome to increase people's income. Salak fruit contains quite complete nutritional elements such as energy, protein, carbohydrates, calcium, phosphate, iron, vitamin B, vitamin C, and water. To reduce the damage of fruit, the fruit of bark is processed into nata that has high nutritional and economic value. Making nata de Salacca assisted by microorganisms namely bacteria Acetobacter xylinum. In this case the microbes play an important role to overhaul substances that are difficult food absorbed by the body into food substances that are easily absorbed by the body. In addition, microbes provide flavor to the processed products. Methods: The duration of fermentation used in this study was 10 and 14 days. To determine the taste of nata de Salacca that has been processed then performed organoleptic test against 15 panelists. Result: The result is a brownish-white color, a slightly acidic sense of conformity, a chewy texture and a slightly acid-scented aroma, so there is a long-standing fermentation effect on the taste of nata de Salacca. Conclusion: The duration of fermentation has an effect on taste of nata de Salacca covering aspect of color, flavor, texture and aroma
POTENSI EKSTRAK DAUN TERATAI (Nymphaea pubescens L.) DALAM MENGHAMBAT Staphylococcus aureus Alfia Sabban; Dominggus Rumahlatu; Theopilus Watuguly
BIOPENDIX Vol 3 No 2 (2017): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue2page129-141

Abstract

Background: The lotus (Nymphaea pubescens L.) is one of the ornamental plants that many people in demand because it has varieties, shapes, and color of flowers are diverse. The lotus has the potential to be developed as an antibacterial. This study aims to determine the use of lotus leaf extract as anti-bacterial Staphylococcus aureus. Method: This study included the making of extraction using maceration method using ethanol, phytochemical testing of lotus leaf extract and testing of anti bacterial activity was done by diffusion method agar by observing and measuring the inhibition zone diameter formed on Muller Hinton (MHA) media. Then done by giving lotus leaf extract with 3 treatment of concentration that is 5%, 10%, 20%, positive control (ampicillin) and negative control (aquades). With an incubation period of 1 x 24 hours. Result: The result of phytochemical test showed that lotus leaf extract contain alkaloid compound, triterpenoid, steroid, flavonoid, phenolic and saponin. Anti-bacterial activity test results showed that the concentration given was not able to inhibit the growth of test bacteria. Conclusions: Bacteriostatic tests with a 60% extract concentration indicating that at 10-5 to 10-9 dilutions there were no bacterial colonies growing.
PENGARUH KONSENTRASI NATRIUM BENZOAT DAN LAMA PENYIMPANAN TERHADAP MUTU MINUMAN SARI BUAH GANDARIA (Bouea macrophylla Griff) BERKARBONASI Rence Aurelia Sumanik; Pamella Mercy Papilaya; Dominggus Rumahlatu
BIOPENDIX Vol 4 No 1 (2017): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol4issue1page22-28

Abstract

Background: Gandaria plants (Bouea macrophylla Griff) are one of the annual Maluku fruits which need to be cultivated because they are beneficial both economically and ecologically. This study aims to determine the effect of sodium benzoate concentration and storage time on Gandaria fruit juice (Bouea macrophylla Griff). Method: The type of research used in this study was an experimental type laboratory with experiments conducted in a closed room. This study uses ripe gandaria fruit in orange. This study used a completely randomized design method with 2 factors, namely, the concentration of sodium benzoate 200 ppm, 250 ppm, 300 ppm and storage time of 5 days, 10 days and 15 days. The parameters measured were vitamin C levels, total solid soluble and total acid levels. Results: The study showed that the concentration of sodium benzoate and storage time had an effect on vitamin C, total acid and total soluble solid. Whereas the interaction between the concentration of sodium benzoate and the length of storage gave a significant effect on total acid. Conclusion: The concentration of sodium benzoate and storage time have an effect on vitamin C, total acid and total soluble solid and the interaction between sodium benzoate concentration and storage time has a significant effect on total acid.
PENGARUH SUHU DAN LAMA FERMENTASI TERHADAP UJI ORGANOLEPTIK PADA PEMBUATAN NATA BUAH ENAU (Areng pinnata Merr) Marselia Latumahina; Ali Awan; Dominggus Rumahlatu
BIOPENDIX Vol 4 No 1 (2017): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol4issue1page29-37

Abstract

Background: Enau is one of the natural resources in the tropics, its distribution is widespread, very necessary and easy to obtain for daily use by local people as a sustainable natural resource. Palm fruit in terms of chemical composition has a low nutritional value, but "kolang-kaling" fiber is very good for health, to reduce fruit damage, then palm fruit is processed into nata which has a high economic value. Method: Making nata de arenga pinnata Merr is aided by microorganisms, namely the bacterium Aceetobacter xylinum. The type of research used is the type of experimental research that is to see the difference in the organoleptic test scores of palm fruit (Arenga pinnata Merr). This research was conducted on December 5 to December 20, 2016. Results: The results showed that organoleptic tests had white, tasteless, chewy texture and a non-sour aroma. Conclusion: There is an influence of temperature with fermentation time on organoleptic test values ​​on the manufacture of palm fruit nata, namely the color and texture of palm fruit nata. Whereas in taste and aroma there is no influence of temperature and duration of fermentation on organoleptic test values ​​on the making of palm fruit nata.
PENERAPAN MODEL PEMBELAJARAN STAD DENGAN MEDIA TEKA-TEKI SILANG DAN MODEL PEMBELAJARAN DISCOVERY LEARNING KONSEP SISTEM GERAK PADA MANUSIA UNTUK MENINGKATKAN HASIL BELAJAR SISWA KELAS VIII SMP NEGERI 1 AMBON Yuliana A Maselkosu; Dominggus Rumahlatu; S I. A. Salmanu
BIOPENDIX Vol 4 No 2 (2018): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol4issue2page70-76

Abstract

Background: The STAD model is to motivate students to support each other and help one another in mastering the abilities taught by the teacher. Crosswords are a game of sharpening the brain where we have to fill in the blank spaces (in the form of white boxes) with the letters that form a word based on the instructions given. Method: Design of experimental research with the type of research used is descriptive comparative. The research subjects were the eighth grade students of Ambon 1 Junior High School, totaling 26 students. The instruments used were initial test and final test questions, observation sheets, student worksheets and group worksheets. Results: Research shows that the application of the STAD Cooperative type learning model with Cross Puzzle media can improve student learning outcomes. On average all aspects of value have increased. Can be seen in the achievement of the estimated student score of 83.46%. Continued hypothesis testing using the t-test with the value of thit> ttab (2.27> 2.01) thus there are differences in the improvement of student learning outcomes using the STAD type cooperative learning model with a crossword puzzle media. Conclusion: There are differences in the application of the STAD learning model with crossword media and discovery learning learning models of motion systems concepts in humans to improve learning outcomes of class VIII students of SMPN 1 Ambon, it is evident that the results of t-test analysis where t count> t table that the STAD model with media crossword puzzles is more effective for improving student learning outcomes than the Discovery Learning model
PENERAPAN MODEL PEMBELAJARAN COURSE REVIEW HORAY UNTUK MENINGKATKAN HASIL BELAJAR SISWA MATERI SISTEM KLASIFIKASI MAKHLUK HIDUP PADA KELAS X SMA NEGERI 14 AMBON Christin Tulaseket; F Leiwakabessy; Dominggus Rumahlatu
BIOPENDIX Vol 4 No 2 (2018): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol4issue2page77-82

Abstract

Background: This study aims to determine the effect of the application of the learning model of the horay course review to improve student learning outcomes of teaching material on the classification system of living things in class X of SMA Negeri 14 Ambon. Method: Samples from this study were students of class X1 with 23 students. This type of research is descriptive research to see student learning outcomes which consists of 3 factors, namely physical, cognitive and psychomotor. The instruments used in this study were test instruments in the form of initial tests and final tests. Results: The results of this study show that there is a significant effect by using the learning review horay learning model on improving student learning outcomes during the implementation of the learning model of the course review horay. Conclusion: Based on the results of the study it can be concluded that the use of the Course Review Horay learning model can improve the learning outcomes of class X1 students at SMA Negeri 14 Ambon. This can be seen from the results of the final test which is very satisfying from the results of the initial test. Presentation of student grades during the initial test is 13% and the final test score reaches 100%.
Co-Authors Alfia Sabban Ali Awan Ali Djamhuri Aloysius Duran Corebima Anggi Wawan Batuwael Aska, Aska Bijaya Latupono Bula, Wiwien Christin Tulaseket Elwuar, Susan Estevanus Kristian Huliselan F Leiwakabessy Fitrin Fredy Leiwakabessy Friescilya Beatrix Latuperissa Izaack, Anaci Since Jeanne L Maalalu Johanis F. Rehena Junita Salelatu Karuwal, Ritha L Kouwe, Rasni Latupono, Bijaya Lessil, Serly Liline, Sintje Liline, Sintje Louisiana Latupeirissa, Louisiana Marselia Latumahina Meganita Meganita Melvie Talakua Mira Wabula Mohamad Amin Nettana, Amro Bernard Pamella Mercy Papilaya Pelamonia, Johanes Pieter Siahaya Prelly M.J Tuapattinaya Rahaweman, Ayu Christien Rehena, Johanis Fritzgal Rence Aurelia Sumanik Renyaan, Angelica Jenita Felisitas S I. A. Salmanu Sangur, Kristin Sekewael, Usi Sintje Liline Siti S. Narahaubun Siti Zubaidah Sriyanti Imelda A Salmanu Sumah, Vonissa Theopilus W Watuguly Tuaputty, Hasan Warella, Juen Carla Yuliana A Maselkosu