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All Journal Archive of Community Health Buletin Udayana Mengabdi Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Jurnal Ilmu Lingkungan Jurnal Kesehatan Masyarakat (JKM) CENDEKIA UTAMA Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Jurnal Gizi Klinik Indonesia ISM (Intisari Sains Medis) : Jurnal Kedokteran PREPOTIF : Jurnal Kesehatan Masyarakat Public Health and Preventive Medicine Archive
Ni Made Utami Dwipayanti
Program Studi Magister Ilmu Kesehatan Masyarakat, Fakultas Kedokteran, Universitas Udayana, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Jurnal Veteriner

 1 
Eksopolisakarida dari Lactobacillus sp. Isolat Susu Kuda Sumbawa dan Potensinya sebagai Prebiotik (EXOPOLYSACCHARIDES FROM LACTOBACILLUS SP. ISOLATED FROM SUMBAWA MARE’S MILK AND ITS POTENTIAL APPLICATION AS PREBIOTICS) I Nengah Sujaya; Ni Putu Desy Aryantini; Ni Wayan Nursini; Cok. Istri Dewiyani Cakrawati; Ni Luh Made Ema Juliasari; Ni Made Utami Dwipayanti; Yan Ramona
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to isolate exopolysaccharides (EPS) producing Lactobacilli isolated fromsumbawa mare’s milk and its potential as prebiotics for modulating the growth of Bifidobacteriumbreve. Nine strains of Lactobacillus sp. were screened for their capabilities to produce EPS usingmodified MRS medium containing sucrose. Prebiotics potential of the EPS was verified by culturingB. breve JCM1273 in TOS medium containing EPS. The results showed that all strains ofLactobacillus sp. produced EPS on MRS sucrose medium and two strains (Lactobacillus SK3.1 andLactobacillus SK4) produced more EPS compared to the other strains tested. Bifidobacteriumbreve JCM1273 showed weak activity while in direct metabolism of EPS produced by Lactobacillussp. SK4 and its growth was enhanced on acid hydrolyzed EPS. Since this phenomenon mighthappened when the EPS exposed by the low pH during gastric passage, hence the EPS might be apotential source to be developed as prebiotics. Nevertheless, further investigation is necessary toevaluate the bifidogenic affects of EPS in Lactobacillus sp. SK4.
CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM SUMBAWA MARE MILK Nengah Sujaya; Yan Ramona; Ni Putu Widarini; Ni Putu Suariani; Ni Made Utama Dwipayanti; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to isolate and characterize lactic acid bacteria (LAB) from the Sumbawa mares milk The Isolation of LAB was conducted in Man Rogosa Sharpe (MRS) agar. The isolates were characterized by standard methods, such as Gram staining, cell morphology study and fermentation activities. The ability of the isolates to inhibit some pathogenic bacteria was studied by dual culture assay. Isolates showing the widest spectrum of inhibiting pathogenic bacteria were further identified using API 50 CHL. The results showed that Sumbawa mare milk was dominated by lactobacilli and weisella/leuconostoc. As many as 26 out 36 isolates belong to homofermentative lactobacilli and another 10 isolates belong to both heterofermentative lactobacilli and weissella or leuconostoc. Twenty four isolates inhibited the growth of Escherichia coli 25922, Shigela flexneri, Salmonella typhimurium, and Staphylococcus aureus 29213. Two promising isolates with the widest spectrum of inhibiting pathogenic bacteria, Lactobacillus sp. SKG34 and Lactobacillus sp. SKG49, were identified respectively as Lactobacillus rhamnosus SKG34 and Lactobacillus ramnosus SKG49. These two isolates were specific strains of the sumbawa mare milk and are very potential to be developed as probiotic for human.
PROBIOTIC POTENCY OF LACTOBACILLUS SPP. ISOLATED FROM SUMBAWA MARE MILK I Nengah Sujaya; I Made Utami Dwipayanti; Ni Luh Putu Suariani; Ni Putu Widarini; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was deigned to elucidate the potency of Lactobacillus spp. isolated from sumbawa mare milk to be developed as a probiotic. Sixteen lacobacilli were screened based on their resitancy to a model of gastric juice at pH 2, 3, and 4, then followed by their resistncy to small intestional fluid model containing deoxycholic. Three lactobacilli i.e. Lactobacillus sp. SKA13, Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were found to be resistentent to gastric juice at pH 3 and 4. However, there were no lactobacilli resisted to pH 2. Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were able to reach the colon even after being expossed to a model of intestinal fluid containing 0,4 mM deoxycholate and pancreatine. Therefore, these isolates have a potency to be developed as probiotic lactobacilli. Nevertherless, these lactobcailli could probably transform cholic acid into secondary bile acids, which were not expected to be found in the probiotic, and this capability is not appropriate for probiotic. This character is worthly to be studied since it has never been reported in lactobacilli.
Production of Biogas As An Alternative Green Energy with Organic Wastes As The Main Raw Materials Yenni Ciawi; Ni Made Utami Dwipayanti; Anak Agung Gede Indraningrat; Yan Ramona
Jurnal Veteriner Vol 24 No 1 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.1.138

Abstract

This research focused on the utilization of four different organic wastes, namely snake fruit, orange, cabbage, and tomato wastes, for the production of biogas. The main objectives were twofold: (1) to investigate the characteristics and biodegradability of these wastes, and (2) to evaluate their potential for anaerobic methane production. The experiment was conducted using 250 L bioreactors, with the four wastes serving as the primary raw materials. A starter culture of cattle dung was added, and the mixture was incubated for eight weeks. Regular sampling and analysis were carried out to assess water content, biodegradability, specific rate of volatile material reduction, and gas yield. The results showed that the water content of the four waste systems remained relatively consistent throughout the experiment. Biodegradability analysis revealed that all of the wastes were biodegradable, with varying levels of degradation ranging from 23.10 ± 2.89% to 59.84 ± 4.17%. Snake fruit waste exhibited the highest resistance to degradation, while tomato waste was the most easily degradable. Kinetic analysis indicated specific rates of volatile material reduction (µ) of 0.006 ± 0.0006 per day for the most resistant waste and 0.0170 ± 0.0021 per day for the least resistant waste. The incorporation of these four waste types in the biogas production process had a positive effect on gas formation. Therefore, these organic wastes hold promise as valuable resources for biogas production, addressing both the issue of waste accumulation and the energy crisis in an environmentally beneficial manner.
Co-Authors A. Wirawan A.A Istri Agung Mirayani Anak Agung Gede Indraningrat Andira, Ratih Ayu Ardhan Tiestian Wouters Cok. Istri Dewiyani Cakrawati Daniel Beltsazar Jacob dewa gede swastika Dinar Lubis Gusti Nyoman Tri Maha Putra I g. H. Purnama I Gede Herry Purnama I Gusti Ayu Kunti Sri Panca Dewi I Made Agus Gelgel Wirasuta I MADE SUTARGA I Nengah Sujaya I Wayan Gede Artawan Eka Putra Kadek Nuansa Putri Wulandari Kadek Tresna Adhi KOMANG AYU NOCIANITRI L. Wulandari Luh Putu Lila Wulandari Made Ayu Hita Pretiwi Suryadhi Mahayogi, Ni Putu Tirta Dewi Michelle Amazing Grace Rampengan Muliana Rofida N T. Suryadhi N. T. Suryadhi N.P. Desy Aryantini N.W. Nursini Ni Ketut Sutiari Ni Ketut Sutiari Ni Komang Ekawati Ni Luh Made Ema Juliasari Ni Luh Payastiti Yunita Ni Luh Putu Intan Sintya Dewi Ni Luh Putu Suariani Ni Putu Desy Aryantini Ni Putu Gita Saraswati Palgunadi Ni Putu Suariani Ni Putu Widarini Ni Wayan Nursini Putu Ayu swandewi astuti Putu Cintya Denny Yuliyatni S. G. Purnama sang gede purnama Savitri, I Gusti Ayu Devi Setiawan, Ronaldo Damar Sut iari. N K. T. Adhi Widya Astuti Yan Ramona Yenni Ciawi Yenni Ciawi