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All Journal Pharmaciana: Jurnal Kefarmasian UNEJ e-Proceeding JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Science and Technology Indonesia Jurnal Farmasi Sains dan Komunitas SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Majalah Farmasetika Berkala Penelitian Hayati Riset Informasi Kesehatan Indonesian Journal of Pharmacy and Natural Product Media Farmasi Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Riesta Primaharinastiti
Departemen Ilmu Kefarmasian, Fakultas Farmasi, Universitas Airlangga, Jawa Timur, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Development and Validation GC/MS Method for Methamphetamine Analysis in Urine by Miniaturization QuEChERS Soraya Aulia; Riesta Primaharinastiti; Djoko Agus Purwanto
Science and Technology Indonesia Vol. 8 No. 3 (2023): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2023.8.3.451-460

Abstract

This paper explains the development of a quick and easy gas chromatography (GC) approach to identify methamphetamine in urine. This research used gas chromatography with mass spectroscopy and a capillary column TG-5SILMS (5% phenyl methyl siloxane, 30 m x 0.32 x 25 m). The carrier gas flow rate was set at 1.0 mL/minute, the temperature inlet and detector had been set at 300°C, and the oven temperature was programmed to initiate at 50°C and held for 1.5 minutes before being raised to 300°C at a rate 40°C/minute and held for 3 minutes. Sample pre-treatment by modification of the QuEChERS method includes using a relatively large amount of inorganic salt, extraction volume and extraction cycle. The optimal conditions for processing a 400 uL urine sample were 160 mg magnesium sulphate, 40 mg sodium chloride, and 400 uL acetonitrile for organic solvent. According to the validation test, the detection limit for methamphetamine was 0.36 ug/mL; the quantitation limit was 1.09 ug/mL, and the calibration curve followed the regression line. y=1.0489x-3.7914, coefficient (r) was 0.9973. The recovery of the analyte spiked into urine at 5, 7 and 9 ug/mL on average was 100.5±2.33% for intraday dan 93.3±7.21% for interday. The precision was excellent, with an average coefficient of variation of 2.31%. The procedure was applied to four urine samples from drug users and the first abuser (25.51 ug/mL), the second abuser (15.05 ug/mL), the third abuser (17.72 ug/mL) and the last abuser (3.08 ug/mL) were all satisfactorily quantitated.
Optimization and Prevalidation of TLC-Densitometry Method for Fucoidan Analysis in Sargassum sp. Aqueous Extract Laili Irfanah; Mochammad Yuwono; Riesta Primaharinastiti
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 10 No. 2 (2023): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v10i22023.210-216

Abstract

Background: Fucoidan is sulfated polysaccharide that has gastroprotector activity, and it is distributed in brown algae cell walls. Currently, there is no method for fucoidan analysis in compendia. Furthermore, analysis of Fucoidan is proven to be challenging due to the lack of chromophores and its high polarity. Objective: To develop the optimal condition of TLC-Densitometry method for fucoidan analysis in Sargassum sp. aqueous extract and to evaluate the stability of Fucoidan as a preliminary study. Methods: Chromatography was performed on Silica gel 60F254 TLC-plate as a stationary phase. The developed plate was stained with H2SO4 10% in absolute ethanol and heated in oven at 105°C for 15 minutes. Optimization is carried out by determining composition of the mobile phase, analytical wavelength, and spotting volume. Stability test of Fucoidan in standard and extract solution at 0, 4, 8, and 24 hours also 0 and 60 minutes after derivatization. Results: The optimal condition which produces a good separation of Fucoidan was achieved by using n-butanol:methanol: water (10:6:10 v/v/v) as a mobile phase, 400 nm as an analytical wavelength, and 1 µl as a spotting volume. Fucoidan was stable after storage until 24 hours. The stained spots were stable until 60 minutes after derivatization. Conclusion: Optimal condition of the TLC-Densitometry method for Fucoidan analysis was selective and can be applied to stability tests in preliminary study. Fucoidan was stable in standard solution and extracted solution until 24 hours after storage at 4°C, and the stained spots were stable until 60 minutes after derivatization.
Comparative Study of Densitometry and Videodensitometry for Quantitating the Active Pharmaceutical Ingredients Using Thin Layer Chromatography – Systematic Review Firmansyah Ardian Ramadhani; Idha Kusumawati; Riesta Primaharinastiti; Subhan Rullyansyah; Fajar Jamaluddin Sandhori; Hanif Rifqi Prasetyawan
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 10 No. 2 (2023): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v10i22023.141-150

Abstract

Background: Chromatography is one of the analytical techniques widely used for the quality control process in the pharmaceutical industry. One of the analytical methods used in drug analysis is Thin Layer Chromatography (TLC). The analysis process of TLC can be performed using densitometry (scanner) or videodensitometry (videoscan). The principal analysis of densitometry (scanner) is based on the density measured from each spot on the TLC plate using a specific wavelength range, and videodensitometry (videoscan) is performed by taking pictures of the plate using a Visualizer at a specific wavelength. Objective: This review article discusses the application of densitometry and videodensitometry methods for quantitative analysis of pharmaceutical products. Methods: This study was conducted using a systematic review method using the PRISMA statement from January to April 2023. Four databases were searched: PubMed, ScienceDirect, Scopus, and Google Scholar with inclusion criteria: studies on thin layer chromatography analysis using densitometry and videodensitometry. Results: Based on the ten articles in this study, it is known that the active ingredient concentrations in pharmaceutical products can be determined using densitometry and videodensitometry. The statistical analysis results show no significant difference between the two methods' chemical concentrations of active ingredients in pharmaceutical products. Conclusion: TLC densitometry and videodensitometry is a valid methods analysis that can be used for quantitating the active pharmaceutical ingredient concentration in finished pharmaceutical products.
Uji Aktivitas Ekstrak Daun Mengkudu (Morindra citrifolia Linn) dan Scopoletin secara In-Vitro terhadap Bakteri Tuberkulosis Dhilasari, Estu Mahanani; Kusumawati, Idha Kusumawati; Primaharinastiti, Riesta Primaharinastiti
Indonesian Journal of Pharmacy and Natural Product Vol. 2 No. 1 (2019)
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.424 KB) | DOI: 10.35473/ijpnp.v2i1.192

Abstract

Penyakit tuberkulosis disebabkan oleh bakteri Mycobacterium tuberculosis. Secara tradisional mengkudu digunakan untuk pengobatan tuberkulosis. Scopoletin merupakan komponen utama dalam mengkudu, oleh karena itu scopoletin sering dijadikan marker dalam studi farmakokinetik. Tujuan penelitian ini adalah untuk mengetahui aktivitas anti-Mycobacterium tuberculosis (H37RV) ekstrak daun mengkudu dan scopoletin melalui penentuan Konsentrasi Hambat Minimum (KHM). Uji aktivitas antibakteri dan penentuan KHM dari ekstrak etanol 50% daun mengkudu dilakukan dengan metode dilusi agar dengan konsentrasi 1,0×10-4 µg/ml – 5,1×10-11 µg/ml. Uji aktivitas menunjukan bahwa ekstrak etanol 50% daun mengkudu dapat menghambat pertumbuhan Mycobacterium tuberculosis dengan KHM 4,0×10-6 µg/ml. Sedangkan scopoletin dengan konsentrasi yang setara dengan kandungan pada ekstrak tidak menunjukan aktivitas anti-Mycobacterium tuberculosisnya.Kata kunci: Mengkudu, Scopoletin, tuberkulosisTuberculosis (TB) is a disease caused by a bacterium, Mycobacterium tuberculosis. Morinda citrifolia Linn has been found to kill Mycobacterium tuberculosis. Scopoletin is a major component in Morinda citrifolia Linn, therefore scopoletin often used as markers in studies of pharmacokinetic. This research purpose to determine anti-Mycobacterium tuberculosis (strain H37RV) activity based on the value of Minimum Inhibitory Concentration (MIC) used to ethanolic extracts from Morinda citrifolia Linn leaf and scopoletin. Experiment of anti-Mycobacterium tuberculosis activities tested by well dillution methods with a dose 1,0×10-4 µg/ml – 5,1×10-11 µg/ml . The results showed that ethanolic extract Morinda citrifolia Linn leaf  were found to be active to Mycobacterium tuberculosis activity with MIC 4×10-6 µg/ml while scopoletin at the same concentration with extract had no anti­- Mycobacterium tuberculosis activity.Keywords:  Noni, scopoletin, tuberculosis
A Comparative Study of Randu Honey Antimicrobial Activity from Several Regions in Java Nindya Pramesti Wardani; Primaharinastiti, Riesta; Achmad Toto Poernomo; Alfi Khatib
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 11 No. 2 (2024): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v11i22024.147-155

Abstract

Background: Randu honey is monofloral honey sourced from a type of plant nectar. The geographical location of randu (Ceiba pentandra) as the source of nectar is one factor that influences the antimicrobial activity of random honey. This research used randu honey from several regions in Java such as Sidoarjo (RSH), Pusat Perlebahan Nasional Bogor (RBH), Kediri (RKH), and Malang (RMH). Objective: To compare the antimicrobial activity of several random honeys (RSH,RBH,RKH, and RMH) against Gram-negative Escherichia coli ATCC 25922, Gram-positive Staphylococcus aureus ATCC 6538, methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33592, and Candida albicans ATCC 10231. Methods: This study used well diffusion and dilution antimicrobial test methods. The diameter of the inhibition zone formed by the well diffusion method was measured using a Vernier caliper. The diffusion method was used as a screening test before determining the quantitative minimum inhibitory concentration (MIC) using serial dilution at a ratio1 of 2 (v/v). Streptomycin and Ketoconazole were used as positive controls. Nutrient broth and Sabouraud broth were incubated at 37°C for 24 h (antibacterial tests) and 25°C for 48 h (antifungal test), respectively. Results: The well diffusion test revealed that all random honey samples could inhibit the test bacteria and fungi with the appearance of an inhibition zone. Diameter inhibition zone ranged from 14.66±0.52 mm to 27.86±0.43 mm. The MICs of RSH,RBH,RKH, and RMH ranged from 3.12% to 25% against all test bacteria and fungi. Conclusion: The results of this study showed randu honey from Bogor (RBH) has the highest antimicrobial activity based on diffusion and dilution tests.
Teknik Aplikasi Sampel Pada Pengujian Kuantitatif Kromatografi Lapis Tipis: Tinjauan Terhadap Area Dan Faktor Retensi Kusumawati, Idha; Primaharinastiti, Riesta; Rifqi Prasetyawan, Hanif
Media Farmasi Vol 20 No 2 (2024): Media Farmasi Edisi Oktober 2024
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v20i2.655

Abstract

Thin Layer Chromatography (TLC) is one of the most commonly used chromatographic techniques to identify compounds in mixtures and determine the purity of a compound. However, the crucial factor that is difficult to control, which can affect the accuracy of the results, is the stage of sample application. This study focused on investigating the effect of different techniques of sample application (manual and semi-automatic) on the area and retention factor (Rf) of the compound spot. Curcuminoid was used as a sample in this study. The manual sample application techniques used a 2μL capillary tube. The semi-automatic sample application techniques used a Camag Linomat-5 instrument with 0, 7, and 8 mm band lengths. After the development of the TLC plate using toluene: acetic acid (4:1, v/v)., the curcuminoid was separated into three spots: curcumin, demethoxycurcumin, and bisdemethoxycurcumin. A one-way ANOVA was used to analyze the spot area and Rf of the three compounds, followed by a Duncan test. The difference in sample application techniques causes differences in the size and shape of the initial sample spot, which affects the compound spot produced after developing of TLC plate. This study showed that the differences in application techniques cause significant differences in the Rf of the compound spot, but do not show significant differences in the compound spot area. Based on this research, optimizing the application techniques of samples is a critical stage and it is essential to optimize how to apply the sample on TLC plate, including the concentration, volume, shape, and size of the spot on TLC.
Antibacterial activity of Randu Honey against some bacterial pathogens using agar well diffusion method Megawati, Megawati; Yuwono, Mochammad; Primaharinastiti, Riesta
Riset Informasi Kesehatan Vol 14 No 1 (2025): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v14i1.954

Abstract

Background: Honey's antibacterial and other therapeutic qualities have long been recognized because of its bioactive components, which include hydrogen peroxide, flavonoids, and phenolics. Randu honey is a monofloral honey that is made from the nectar of Ceiba pentandra and has the ability to suppress Gram-positive as well as Gram-negative bacteria. Because antibiotic resistance is on the rise, it is crucial to investigate alternative treatments like honey. Method: This study evaluates the antibacterial activity of randu honey using the agar well diffusion method or Kirby-Bauer diffusion method against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa to determine its Minimum Inhibitory Concentration (MIC) by preparing a series of honey dilutions ranging from 100% to 10% (w/v) then incubating in an incubator at a temperature of 37°C for 24 hours to allow interaction between the test solutions and bacteria on the agar media, followed by measuring the inhibition zones formed using a caliper. Results: The antibacterial activity of randu honey increased with concentration. Staphylococcus aureus showed the highest sensitivity with an inhibition zone of 9.02 ± 0.67 mm at 100%, classified as moderate at 70%–100% concentrations (MIC 20%). Escherichia coli exhibited lower sensitivity, with inhibition zones ranging from 7.07 ± 0.56 mm at 100% to 0.18 ± 0.01 mm at 30%, categorized as moderate at 80%–100% (MIC 30%). For Pseudomonas aeruginosa, moderate activity was observed at 80%–100%, with inhibition zones up to 8.16 ± 0.13 mm at 100% (MIC 30%). Conclusion: Randu honey shows promising antibacterial activity, especially against Staphylococcus aureus, with concentration-dependent effects. Its moderate activity at higher concentrations highlights its potential as a natural antibacterial agent.
Development of Chemometric Methods for Determining Caffeine Content in Robusta Coffee as Alternative Standardization Techniques Handoyo, Theodorus Rexa; Primaharinastiti, Riesta; Yuwono, Mochammad
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 22, No 2 (2025)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.009242

Abstract

There is a necessity for standardization to uphold coffee quality due to its frequent production and consumption. High-performance liquid chromatography (HPLC) serves as the benchmark for caffeine analysis in coffee in Indonesia. However, its high cost has prompted the exploration of alternative methods. This study aims to obtain a suitable alternative method for determining the caffeine content in robusta coffee as an effort toward standardization. Employing statistical and mathematical techniques in chemistry, chemometrics emerges as a promising alternative in analyzing caffeine in coffee. The approach was integrated with an ultraviolet (UV) spectrophotometer capable of analyzing substances across wavelengths from 200 to 400 nm. From the five replications, the HPLC method showed a caffeine content of 1.435 ± 0.011% (w/w), while the spectrophotometer showed a content of 1.723 ± 0.003% (w/w). Through the partial least squares regression (PLSR) and principal component regression (PCR) methods in RStudio, the results obtained were 1.432 ± 0.003% and 1.430 ± 0.002% (w/w), respectively. These results indicate good REP values of 0.022% for PLSR and 0.155% for PCR. With these findings, it is hoped that chemometrics can become an alternative method for analyzing caffeine in coffee.
AKTIVITAS AGEN TROMBOLITIK DAUN PEGAGAN (Centella asiatica) HASIL FERMENTASI OLEH Acetobacter aceti FNCC 0016 DENGAN METODE CLOT LYSIS SECARA IN-VITRO Adristy Ratna Kusumo; Achmad Toto Poernomo; Riesta Primaharinastiti
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 7 No. 2 (2021): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v7i2.499

Abstract

Thrombolytic agents are plasminogen activators which can dissolve blood clot that caused cardiovascular disease (CVD). Thrombolytic agents can be obtained from microorganism such as Acetobacter aceti FNCC 0016 and from plants such as Centella asiatica, these thrombolytic agents are combined using fermentation method. Fermentation can improves the pharmacological properties of plants through modification of their metabolites. Fermentation mediated by microorganism has been shown to enhance the therapeutic efficacies of some plants. This research aimed to determine the increase of thrombolytic activity from the fermentation product of Centella asiatica by Acetobacter aceti FNCC 0016. Fermentation was carried out at various times (0 h, 24 h, 48 h, 72 h) at a temperature of 30±1°C. The thrombolytic activity was determined using in-vitro clot lysis method. The results showed a significant increase in thrombolytic activity after 72 hours of fermentation with thrombolytic index of 91,49 compared to Centella asiatica extract (42.93) and Acetobacter aceti FNCC 0016 (19.99). Conclusion: Thus, Centella asiatica that has undergone a fermentation process with Acetobacter aceti FNCC 0016 can increase the thrombolytic activity.
Pembentukan pojok herbal dan produk herbal (teh dan sirup) untuk meningkatkan kualitas kesehatan masyarakat di Desa Bengkaung, Kecamatan Batulayar, NTB Aty Widyawaruyanti; Suciati Suciati; Tri Widiandani; Agriana Rosmalina Hidayati; Achmad Fuad Hafid; Djoko Agus Purwanto; Neny Purwitasari; Riesta Primaharinastiti; Sukardiman Sukardiman; Retno Widyowati; Devanus Lahardo; Hilkatul Ilmi; Maylisa Natalia Corry; Firman Wicaksana; Narendrani Sasmitaning Edhi; Muhammad Amir Hasan
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 9, No 3 (2025): May
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v9i3.30199

Abstract

Abstrak Desa Bengkaung adalah desa yang terletak di kecamatan Batu Layar, Lombok Barat, Nusa Tenggara Barat.  Banyak potensi pada desa Bengkaung yang masih bisa dikembangkan diantaranya adalah sumber daya alam yang berlimpah. Namun, pemanfaatan sumber daya alam ini belum maksimal, yang disebabkan oleh kurangnya pengetahuan masyarakat mengenai manfaat dan  pengolahannya. Maka dilakukan pengabdian masyarakat yang berlangsung 2 tahap yaitu seminar terkait pojok herbal dan pelatihan pembuatan teh dan sirup dengan bahan kayu manis, kunyit, temu lawak, asam jawa, kapulaga untuk sediaan sirup serta bangle pada sediaan teh herbal yang berfungsi untuk meningkatkan kesehatan masyarakat. Kegiatan tersebut dilaksanakan  pada 25 Oktober 2024 dengan mitra terdiri dari  anggota IAI  kota Mataram dan kader PKK desa Bengkaung. Adapun materi pelatihan diberikan dengan metode ceramah dan metode praktik. Evaluasi dilakukan dengan cara pemberian pre-test dan post-test, kemudian dianalisis secara kuantitatif. Berdasarkan hasil pre-test dan post-test, diperoleh nilai rata-rata sebesar 60,94 dan 79.37, yang menunjukkan adanya peningkatan pengetahuan mitra terhadap materi penyuluhan yang diberikan. Teh daun salam dan JKP-Bangle dievaluasi dengan pengukuran kadar antioksidan dengan DPPH dan FRAP assay. Hasil analisis menunjukkan bahwa kadar antioksidan the daun salam dan JKP-Bangle dengan FRAP assay memperoleh kadar tertinggi tertinggi nilai masing-masing sebesar 1359.84±13.47 mg GAE/g berat kering dan 396.73±16.79 mg GAE/g berat kering. Aktivitas antioksidan yang tinggi ini mengindikasikan potensi manfaat kesehatan dalam menangkal stres oksidatif yang berhubungan dengan penyakit neurodegeneratif, kardiovaskular, dan diabetes. Kata kunci: Desa Bengkaung; pojok herbal; tanaman obat; antioksidan. Abstract Bengkaung Village is a village located in Batu Layar sub-district, West Lombok, West Nusa Tenggara.  There is a lot of potential in Bengkaung Village that can still be developed, including abundant natural resources. However, the utilization of these natural resources has not been maximized, this is caused by a lack of public knowledge regarding their benefits and processing. So community service was carried out which took place in 2 stages, namely seminars related to the herbal corner and training in making tea and syrup using cinnamon, turmeric, ginger, tamarind, cardamom for syrup and bangle preparations, as well as bangles for herbal tea preparations which function to improve health. public. This activity was carried out on 24-26 October 2024 with partners consisting of IAI members from Mataram city and PKK cadres from Bengkaung village. The training material is provided using the lecture method and the Experimental Learning method (direct implementation learning). Evaluation is carried out by giving a pre-test and post-test, then analyzed quantitatively. Based on the results of the pre-test and post-test, an average score of 60.94 and 79.37 was obtained, which indicates an increase in partners' knowledge of the extension material providedrn how to make innovative products from mangosteen fruit so that they can be produced commercially. Thus, in the long term it is expected to increase the income of the Songgon Village residents. Antioxidant levels of bay leaf tea and JKP-Bangle were evaluated using DPPH and FRAP assays. Analysis results showed that the highest antioxidant levels measured by the FRAP assay were 1359.84±13.47 mg GAE/g dry weight for bay leaf tea and 396.73±16.79 mg GAE/g dry weight for JKP-Bangle. This high antioxidant activity indicates potential health benefits in combating oxidative stress associated with neurodegenerative, cardiovascular, and diabetes-related diseases. Keywords: Songgon Village; herbal corner; medicinal plants; antioxidants.
Co-Authors A. Toto Poernomo Achmad Fuad Hafid Achmad Toto Poernomo Adristy Ratna Kusumo Alfi Khatib Annuryanti, Febri Asih Imulda Hari Purwani Asri Darmawati Aty Widyawaruyanti Bambang Tri Purwanto Devanus lahardo Djoko Agus Purwanto Estu Mahanani Dhilasari Etik Wahyuningsih Fajar Jamaluddin Sandhori Firman Wicaksana Firmansyah Ardian Ramadhani Hanif Rifqi Prasetyawan Hidayati, Agriana Rosmalina Hilkatul Ilmi Idha Kusumawati Ika Rizki Helwandi Isnaeni Lailatul Nuraini Laili Irfanah Maylisa Natalia Corry Megawati - Mochamad Yuwono Mochammad Yuwono Muhammad Amir Hasan Muhammad Hidyatullah Narendrani Sasmitaning Edhi Nindya Pramesti Wardani Noor Erma S. Prasetyawan, Hanif Rifqi Purwitasari, Neny Rachma Ayuningtyas Rachma Nurhayati Retno Widyowati Soraya Aulia Subhan Rullyansyah Suciati Suciati Sukardiman Sukardiman Sulistyianingsih Syahrani, Achmad Theodorus Rexa Handoyo Tri Widiandani