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All Journal Jurnal Teknologi Dan Industri Pangan VALENSI Jurnal Ilmu Lingkungan AL KAUNIYAH Alchemy : Journal of Chemistry Bulletin of Chemical Reaction Engineering & Catalysis Proceeding International Conference on Global Resource Conservation JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Elkawnie EduChemia: Jurnal Kimia dan Pendidikan ALCHEMY Jurnal Penelitian Kimia Jurnal Kimia Terapan Indonesia Jurnal Riset Sains dan Kimia Terapan Bulletin of Chemical Reaction Engineering & Catalysis al Kimiya : Jurnal Ilmu Kimia dan Terapan
Dede Sukandar
Chemistry Department, Science And Technology Faculty, UIN Syarif Hidayatullah Jakarta Jl. Ir. H. Djuanda No. 95, Ciputat, Tangerang Selatan, Banten, Indonesia 15412
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Veterinary Other

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Journal : Elkawnie

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Isolation and Cytotoxic Activity of The β-Carotene Combination of Trigona Honey and Namnam Leaves Extract (Cynometra cauliflora) Ahmad Fathoni; La Ode Sumarlin; Fuady Hanief; Dede Sukandar
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 1 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i1.8696

Abstract

Abstract: Isolation and cytotoxic activity of the β-carotene combination of Trigona honey and Namnam leaves extract (Cynometra cauliflora) were conducted. The urgency of isolating β-carotene compounds because of their known anticancer activity. Namnam leaves are macerated using methanol, then combined with Trigona honey. Fractionation and isolation of β-carotene in combination samples were conducted out by chromatography method. The β-carotene was analyzed using UV-Vis and FTIR spectrophotometer. The cytotoxic activity test was conducted by using the Brine Shrimp Lethality Test (BSLT) method with mortality analysis using Probit Analysis with SPSS v20 and Lethal Concentration 50 (LC50) as parameters. The result showed that the cytotoxic activity of Trigona honey after combination reached 36.6% with an LC50 value of 168.2 ppm, an increase from the Trigona honey sample (LC50 = 265.2 ppm) and Namnam leaves extract (LC50 = 196.12 ppm). The highest cytotoxic activity belongs to the n-hexane fraction (LC50 = 77.6 ppm). The best eluent for β-carotene isolation is petroleum ether. There was increased cytotoxic activity in isolates (LC50 of 22.85 ppm). Isolates were analyzed by UV-Vis and FTIR and compared with standards compound. FTIR analysis results showed that the isolates had functional groups of -CH3 (v 2850-2960 cm-1), -CH3 aliphatic (v 1350-1470 cm-1), C=C (v 2100-2350 cm-1 and 675-870 cm-1), and C-O (v 1000-1300 cm-1). Thus, the combination of Trigona Honey and Namnam leaf extract can be developed as an alternative chemopreventive agent.Abstrak: Isolasi dan aktivitas sitotoksik β-karoten kombinasi madu trigona dan ekstrak daun namnam (Cynometra cauliflora) telah dilakukan. Urgensi mengisolasi senyawa β-karoten karena telah diketahui adanya aktifitas antikanker. Daun namnam dimaserasi menggunakan metanol, untuk kemudian dikombinasikan dengan madu trigona. Fraksinasi dan isolasi β-karoten pada sampel kombinasi dilakukan dengan metode kromatografi. Kandungan senyawa β-karoten dianalisis menggunakan spektrofotometer UV-Vis dan FTIR. Uji aktifitas sitotoksik dilakukan dengan metode Brine Shrimp Lethality Test (BSLT) dengan analisis mortalitas menggunakan Probit Analysis dengan software SPSS v20 dan Lethal Concentration 50 (LC50) sebagai parameter. Hasil analisis menunjukkan bahwa aktivitas sitotoksik madu trigona setelah dikombinasikan mencapai 36,6 % dengan nilai LC50 sebesar 168,2 ppm, meningkat dari sampel madu trigona dengan (LC50 = 265,2 ppm), dan ekstrak daun namnam dengan (LC50 = 196,12 ppm). Aktifitas sitotoksik tertinggi dimiliki oleh fraksi n-heksana(LC50 = 77,6 ppm). Eluen terbaik untuk isolasi β-karoten adalah petroleum eter. Terjadi peningkatan aktifitas sitotoksik pada isolat hasil pemisahan (LC50 sebesar 22,85 ppm). Isolatdianalisis dengan UV-Vis dan FTIR dan dibandingkan dengan senyawa standar. Hasil analisa FTIR menunjukkan isolat memiliki gugus fungsi -CH3 (v 2850-2960 cm-1), -CH3 alifatik (v 1350-1470 cm-1), C=C (v 2100-2350 cm-1 dan 675-870 cm-1), dan C-O (v 1000-1300 cm-1). Dengan demikian, kombinasi Madu Trigona dan ekstrak daun namnam berpotensi untuk dikembangkan sebagai agen kemoprefentif alternatif.
Co-Authors A Sa’duddin A.S. Satyaningtijas Abbas, Jamillah Achmad Tjahja Adawiah Adawiah Adawiah Adawiah adawiah adawiah, adawiah Adeng Hudaya Adeng Hudaya Adid Adep Dwiatmoko Agik Suprayogi Agustino Zulys Ahmad Fathoni Ahmad Tjahja Nugraha, Ahmad Tjahja Ai Faziah Hayati Alfindah Rusanti Amri, Ghulam Fathul Andriyani, Leni Anna Muawanah Anny Sulaswatty Bale, Uly Fitria Chitta Putri Novianti Dasumiati Dasumiati Eka Rizki Amelia Eka Rizki Amelia Eka Rizki Amelia Eka Rizki Amelia Eka Rizki Amelia Eka Rizki Amelia Elda Suci Yala Merru Emi Lestari Emi Lestari Eva Silvia Fadhilla, Rizka Fadhilla, Rizkha Farishi, Salman Fauzan Aulia Fauzih, Dewi Fitria, Shella Fitriyanti Fitriyanti Fuady Hanief Habibah, Nurfitriany Hadriyani, Rizka Hendrawati Hendrawati Hilyatuz Zahroh Hilyatuz Zahroh Ibnu Umarudin Umedi Idzni Qistina Ika Amalia, Ika Ika Restu Purwanti Imam Hamidi Imamah Al Mabrur Imamah Al Mabrur Ira Djajanegara Ira Djajanegara Ira Jayanegara Ira Jayanegara Isalmi Aziz Khilda Fithri Aryani Khoeriyah Khoeriyah La Ode Sumarlin Lailatul Badriyah Lia Pratiwi Melina Hadera Muhamad Zaenudin Nanda Saridewi, Nanda Nani Radiastuti Nani Radiastuti Nani Radiastutu Nina Artanti Nino Rinaldi Nurhasni Nurhasni Nurlaela Nurlaela Rahminiwati, Min Ramadhan, Fitri Nur Rina Ningtiyas Rista Kurnia Dewi Rizky Achmad Fauzi Saeful Rohman Sandra Hermanto Septyani Nurichawati Sihono Sihono Silvester Tursiloadi Siti Nurbayti Siti Nurbayti Siti Nurbayti Sofnie M Chairul Sri Yadial Chalid Sudiyarmanto Sudiyarmanto Syarifah Utami Tarso Rudiana, Tarso Teguh Wahyono Tiah Maharani Tri Heru Prihadi Trilaksono Trilaksono Tsalats Wahyul Husna Tulhusna, Latifah W. Kurniawan Wahyudin Wahyudin Wawan Rustyawan Widad Basalamah Yulyani Nur Azizah