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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan VALENSI JURNAL KIMIA SAINS DAN APLIKASI Jurnal Ilmiah Aplikasi Isotop Radiasi AL KAUNIYAH Jurnal Kimia dan Kemasan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Elkawnie Jurnal Kimia Terapan Indonesia JRST (Jurnal Riset Sains dan Teknologi) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Molekul: Jurnal Ilmiah Kimia Jurnal Kimia dan Kemasan
Anna Muawanah
Chemistry Departement Faculty Of Science And Technology Islamic State University Syarif Hidayatullah Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Veterinary

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The Total Phenolic, Total Flavonoid, and Brown Pigment in Honey Before and After Heating Achmad Tjachja Nugraha; La Ode Sumarlin; Anna Muawanah; Nurul Amilia; Meyliana Wulandari
Elkawnie: Journal of Islamic Science and Technology Vol 8, No 1 (2022)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v8i1.12757

Abstract

Abstract: Honey has unique components, a characteristic that makes it a valuable food for consumers. It is known that UV radiation and heating impact the quality of honey's bioactive compounds, including total phenolic, total flavonoid, and brown pigment contents. The absorbance of brown pigment at a wavelength of 420 nm, total phenolic at a wavelength of 733 nm, and total flavonoid at a wavelength of 430 nm was measured using a UV-Vis spectrophotometer. The method used in the total phenolic test was the Folin-Ciocalteu method with gallic acid standard (mgGAE/g sample). In contrast, for flavonoids, the method used was the colorimetric method with quercetin standard (mg QE/g sample). The qualitative test results showed that all honey samples contained phenolic and flavonoid compounds. Total phenolic and flavonoid levels showed a decrease in several samples, including TR SLT (Trigona Southeast Sulawesi), TRG BGR (Trigona Bogor), and TR SLS (Trigona Genotrigona Indica South Sulawesi) in the range of 11.8–57.6%. However, most of the total phenolic and flavonoid levels increased after the heating process, i.e., in the samples of AP LMB (apis North Lombok) (25.3% and 88.8%), AP MG (apis mango) (73.1% and 114%), AP MAC (Aceh honey, Buloh Seuma) (8.8% and 199%), TR BIR (Trigona tetroginola biroi South Sulawesi) (58.8% and 146%), and TR LMB (Trigona North Lombok) (44.3% and 84.9%). In contrast, for the formation of brown pigment, there was an increase in all honey samples between 32 and 1.428%. The presence of brown pigment at the end of the heating process of honey samples is thought to have the same role as phenolic compounds and flavonoids, which change the heating process, especially in antioxidant activities and other bioactivities. However, further analysis is needed to prove the conjecture.Abstrak: Madu memiliki komponen yang unik, yang membuatnya menjadi makanan yang berharga bagi konsumen.  Telah diketahui bahwa radiasi UV dan pemanasan berdampak pada kualitas senyawa bioaktif madu, di antaranya kandungan total fenolik, total flavonoid, dan pigmen coklat. Pengukuran absorbansi pigmen coklat pada panjang gelombang 420 nm, pengukuran total fenolik pada panjang gelombang 733 nm dan total flavonoid pada panjang gelombang 430 nm dilakukan menggunakan spektrofotometer UV-Vis. Metode yang digunakan untuk uji total fenolik adalah metode Folin-Ciocalteu dengan standar asam Galat (mgGAE/g sampel), uji flavonoid menggunakan metode Kolorimetri dengan standar kuersetin (mg QE/g sampel).  Hasil uji kualitatif menunjukan bahwa semua sampel madu terdapat senyawa fenolik dan flavonoid. Kadar total fenolik dan flavonoid menunjukkan penurunan pada beberapa sampel, di antaranya TR SLT (Trigona Sulawesi Tenggara, TRG BGR (Trigona Bogor), TR SLS (Trigona Genotrigona Insica Sulawesi Selatan) pada kisaran 11,8 – 57,6%.  Namun, sebagian besar kadar total fenolik dan kadar flavonoid mengalami kenaikan setelah proses pemanasan, yaitu pada sampel AP LMB (apis Lombok Utara) (25,3% dan 88,8%), AP MG (apis mangga) (73,1% dan 114%), AP MAC (madu Aceh, Buloh Seuma) (8,8% dan 199%), TR BIR (Trigona Tetroginola biroi sulawesi selatan) (58,8% dan 146%), TR LMB (Trigona North Lombok) (44,3% dan 84,9), sedangkan untuk pembentukan pigmen coklat, terjadi peningkatan pada semua sampel madu di antara 32 – 1.428%. Keberadaan pigmen coklat pada akhir proses pemanasan, diduga memiliki peran yang sama dengan senyawa fenolik dan flavonoid yang mengalami perubahan selama proses pemanasan, terutama pada aktivitas antioksidan dan bioaktivitas lainnya.  Namun diperlukan analisis lebih lanjut untuk membuktikan dugaan tersebut.
Inhibitory Activity of HEp-2 Cells by Honey from Indonesia La Ode Sumarlin; Anna Muawanah; Farhan Riza Afandi; Adawiah Adawiah
Jurnal Kimia Sains dan Aplikasi Vol 22, No 6 (2019): Volume 22 Issue 6 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3698.421 KB) | DOI: 10.14710/jksa.22.6.317-325

Abstract

Indonesian local honey contains active compounds that have the potential as an antioxidant and anticancer, primarily as a laryngeal anticancer through the inhibition of HEp-2 cells. This study aims to determine the anticancer activity of several types of honey in Indonesia through the inhibition of HEp-2 cells. Samples used in the form of Trigona, Longan, Rambutan, and Kaliandra honey obtained from honey farmers in Sulawesi and Java, Indonesia. Honey samples extracted by using methanol, then liquids partition was carried out consecutively using n-hexane and ethyl acetate. The cytotoxicity test for HEp-2 cells was carried out using the MTT method (3- (4,5-dimethyl thiazol-2-il) -2,5-diphenyl tetrazolium bromide). The results showed that all honey samples were active against preventing HEp-2 cells with the highest inhibition activity from longan honey with ethyl acetate extract at 65.18% at 100 ppm. Longan honey has decreased HEp-2 cell inhibitory activity after fractionation. Indonesian local honey, namely trigona honey, kaliandra honey, rambutan honey, and longan honey, can be used as a supplementary supplement for patients with laryngeal cancer.
Aktivitas Penangkapan Radikal DPPH (1,1-Diphenyl-2-picrylhydrazyl) Ekstrak Madu Sebelum dan Sesudah Pemanasan La Ode Sumarlin; Anna Muawanah; Amalia Istiqomah; Nurul Amilia; Tarso Rudiana; Sri Yadial Chalid; Hajar Hajar
JRST (Jurnal Riset Sains dan Teknologi) Volume 7 No. 2 September 2023: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v7i2.16855

Abstract

Pemanasan pada suhu dan waktu optimum dapat meningkatkan aktivitas antioksidan pada madu apis dan trigona karena meningkatnya total fenolik, total flavonoid dan pigmen coklat. Kemampuan antioksidan dapat diukur melalui kemampuan menangkap radikal oleh DPPH (1,1-Diphenyl-2-picrylhydrazyl).  Tujuan penelitian ini untuk membandingkan perubahan aktivitas antioksidan melalui parameter penangkapan radikal DPPH serta pigmen coklat pada 5 jenis madu apis dan 5 jenis madu trigona dari kota Bogor, Kalimantan, Sulawesi, dan Lombok pada sebelum dan sesudah dipanaskan yang diukur dengan menggunakan spektrofotometer UV-Vis.  Hasil penelitian ini menunjukkan persen inhibisi baik pada madu jenis apis maupun pada madu jenis trigona, cenderung mengalami peningkatan seiring dengan pertambahan suhu dan waktu pemanasan.  Pigmen coklat didapatkan cenderung mengalami peningkatan seiring dengan pertambahan suhu dan waktu pemanasan.  Kemampuan penangkapan radikal DPPH paling tinggi terdapat pada madu jenis trigona asal Lombok dengan nilai inhibisi 93% dan IC50 sebesar 3.435 ± 29,3 ppm dengan persentase kenaikan 59,1 % pada suhu 50o C selama 9 hari.   Dengan demikian, madu jenis Apis dan Trigona memiliki senyawa bioaktif  dengan kemampuan menangkap radikal DPPH diantaranya terbentuknya pigmen coklat serta berpotensi meningkat kemampuan antioksidannya melalui pemanasan pada suhu tertentu.
Natural Zeolite as Mo and MoP Catalysts Support for Catalytic Deoxygenation of Jatropha Oil Aziz, Isalmi; Farhan, Muhammad; Saridewi, Nanda; Azizah, Yulyani Nur; Muawanah, Anna; Nurbayti, Siti; Dwiatmoko, Adid Adep; Adhani, Lisa
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.45272

Abstract

Non-edible oil, such as Jatropha oil, is an interesting feedstock for the development of renewable diesel (green diesel). Catalytic deoxygenation using natural zeolite-supported Mo-based catalysts is a promising process for the conversion of Jatropha oil to green diesel. Mo and MoP catalysts supported on natural zeolite were synthesized by wet impregnation at a concentration of 5% (w/w). The catalysts were characterized by XRD, XRF, SAA and NH3-TPD. The catalysts were successfully synthesized with the appearance of Mo and MoP peaks on the catalyst diffractogram. XRF results also showed that Mo and P were present in the catalyst. Metal impregnation decreased the surface area and pore volume of the catalyst, but increased the average pore diameter. The NH3-TPD profile of the catalyst showed that the weak acid sites of both catalysts were larger than the strong acid sites. Based on the activity test of catalytic deoxygenation of Jatropha oil, the MoP/HZ catalyst produced a higher conversion (67%) and liquid product yield (79%) than Mo/HZ. This is associated with a larger pore diameter, so that the distribution of reactants on the catalyst surface is more optimal. However, the highest green diesel selectivity of 82% is produced by the Mo/HZ catalyst. The Mo/HZ catalyst is more oriented towards the HDO reaction, whereas the MoP/HZ catalyst is more oriented towards the DCO/DCO2 reaction.
Co-Authors . Masitoh A Sa’duddin Achmad Tjachja Nugraha Adawiah Adawiah Adawiah Adawiah Adeng Hudaya Adeng Hudaya Amalia Istiqomah Anisa Ulfatu Aeni Dadang Sudrajat Dede Sukandar Dedi Ansori Dewi Sondari Dian Aprianti Dwiatmoko, Adid Adep Eka Rizki Amelia Endang Sri Heruwati Farhan Riza Afandi Farida Ariyanti Fazhar Akbar Hajar Hajar Hajar Hanny Hafiar Hatiningsih, Fitriah HUSNUL KHOTIMAH Ida Jubaedah Ira Djajanegara Ira Jayanegara Isalmi Aziz Khilda Fithri Aryani La Ode Sumarlin La Ode Sumarlin La Ode Sumarlin Lisa Adhani Megga Ratnasari Pikoli Meyliana Wulandari Muhammad Farhan Nadya Uswatun Hasanah Nana Mulyana Nanda Saridewi, Nanda Nani Radiastuti Nani Radiastutu Nurul Amilia Nurul Amilia Nurul Amilia Prita Wardhani Prita Wardhani Putera, Deni K Rahmawati Putri Rizkina Harahap Sandra Hermanto Septiana, Annisa Sihono Sihono Siti Nurbayti Sri Fahmiati Sri Yadial Chalid Sri Yadial Chalid Teguh Wahyono Tri Retno Diah Larashati W. Kurniawan Wida Banar Kusumaningrum Widad Basalamah Widia Apriliani Yulianti Sampora Yulyani Nur Azizah