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Journal : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

KARAKTERISTIK MORFOMETRIK DAN MERISTIK IKAN LAIS (Kryptopterus limpok, Bleeker 1852) DI SUNGAI TAPUNG DAN SUNGAI KAMPAR KIRI PROVINSI RIAU Elis Suryana; Roza Elvyra; Yusfiati '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Tapung river and Kampar Kiri river have different characteristics.Tapung river have uncontaminated ecosystem waters, while Kampar Kiri river has been already contaminated. The different condition of two rivers influence fish morphometric and meristic. The purpose of this research is to determine the difference between morphometric and meristic of male and female lais fish in Tapung river and Kampar Kiri river. The fish samples have been taken on March-May 2014. As many as 60 fish (consisting of 30 males and 30 females) were collected every month for each location. The data were analyzed using Microsoft Excel and SPSS version 16. Morphometric characters of male and female lais fish K.limpok in Tapung river have longer size than in Kampar Kiri river. The growth status of lais fish on each observation station werepositive allometric, negative allometric and isometric. T-tes showed that morphometric between male lais fish and female lais fish for each location of the research were significant different. The meristic of male and female lais fish in both rivers were not have significant different.
ANALISIS ISI LAMBUNG IKAN TAPAH (Wallago leeri) DI PERAIRAN SUNGAI SIAK DAN SUNGAI KANDIS DESA KARYA INDAH KECAMATAN TAPUNG Susi Kemala Sari; Roza Elvyra; Yusfiati '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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A study on gut content analysis of Tapah fish (Wallago leeri) at two stations of Siak andKandis river has been carried out in February to July 2013. The objective of this study wasto know about the kinds of food for Tapah fish,such as main, suplement and additional foodin Siak and Kandis river. The data was analyzed using the Index of Preponderance (thelargest part index) by Natarjan and Jingran cit. Effendie (1979). The result of thestudyrevealed that the main food of Tapah fish in Siak river station consists ofCyclocheilichthyssp. (48%), the supplement food consists of Ompok sp. (28%), Puntius sp.(12%), and animal debris (11%), whereas additional food is Rasbora sp. (1%). Tapah fishin Kandis river has main food which consists of Cyclocheilichthys sp. (43%), and supplementfood consists of Ompok sp. (27%), Puntius sp. (10%), and animal debris (18%). As anadditional food, Tapah fish also feeds on Rasbora sp. (2%).
TEKNIK ISOLASI DAN ELEKTROFORESIS DNA TOTAL PADA Kryptopterus apogon (Bleeker 1851) DARI SUNGAI KAMPAR KIRI DAN TAPUNG HILIR KABUPATEN KAMPAR PROVINSI RIAU Dede Aryani Novitasari; Roza Elvyra; Dewi Indriyani Roslim
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Kryptopterus apogon is one of freshwater fish species that was only found in floodplain river in Riau Province. Many species of lais fish are found in Riau Province rivers and morphologically has the same shape therefore it is necessary to find alternative markers to distinguish the species. The important step in genetic analysis is DNA isolation. This study aimed to isolate the total DNA from Kryptopterus apogon. The fish samples were taken from the Kampar Kiri and Tapung Hilir rivers. Isolation of total DNA was done by taking the muscle tissue from the fish tail (1 mm x 1 mm in size). Isolation of total DNA was done using DNA isolation kit (QIAGEN DNeasy Blood & Tissue). After that, the total DNA was checked on 1% agarose gel to estimate the quantity and quality of total DNA. Total isolated DNA of Kryptopterus apogon from two rivers had various quality and quantity. Tha total quantity of DNA molecules ranged from to 100-200 ng/μl, while the total quality was good enough or not fragmented. The DNA was clear, thick, and no smear.
MORFOMETRIK DAN MERISTIK IKAN BUNTAL MAS (Tetraodon fluviatilis H.B.) DI MUARA PERAIRAN BENGKALIS PROVINSI RIAU Tri Setia Ningsih; Roza Elvyra; Yusfiati '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Morphometric and meristic analysis of pufferfish (Tetraodon fluviatilis H.B.) in estuary of Bengkalisconducted in April-August 2014, which aims to determine the characteristics morphometric andmeristik pufferfish (T. fluviatilis H.B.) in three different habitats. This study used the method of direct data collection sampling from study sites. Sampling was carried out at three different stations are three i.e Pakning River, Bengkel River Station and the Jangkang River with 30 male and 30 female. The results showed morphometric characters pufferfish gold female and male on the Pakning River greater than the Bengkel River and Jangkang River with status allometric growth is positive, negative allometric and isometric. The results showed of morphometric analysis pufferfish (T. fluviatilis H.B.) using T-test showed a significant difference in the pufferfish gold male and female at each study site. Analysis of meristik pufferfish gold male and female at each study site showed a significant difference.
STRUKTUR INSANG IKAN Ompok hypophthalmus (Bleeker 1846) DARI PERAIRAN SUNGAI SIAK KOTA PEKANBARU Dwi Indrayani; Yusfiati '; Roza Elvyra
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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This study aimed to observe the pathology of fish gill tissue Ompok hypophthalmus from Siak River around Siak I and Siak II bridge, Pekanbaru. The research was conducted from December 2013 to April 2014. The samples were prepared for histological observation using paraffin method and stained with Hematoxylin-Eosin (HE). Data were analyzed descriptively and quantitatively based on the histopathological evaluation and the value of the damage scoring. The results showed that the gill tissue structure of O.hypophthalmus fish changed. The damage in gill tissue found at Station I were 8,4% hyperplasia, 5,9% lamella fusion, 2,9% odema and 0,9% necrosis with total damage of gill tissue was 18,1%. Meanwhile, the damage in gill tissue found at Station II were 7,1% hyperplasia, 6,1% lamela fusion, 2,4% odema and 0,9% necrosis with total damage of gill tissue was 16,5%. The results of histopathological scoring below ≤25% indicated that the condition of the gill tissue was considered normal.
ASPEK REPRODUKSI IKAN SELAIS KAPOREH (Ompok eugeneiatus) DI SUNGAI KAMPAR KABUPATEN KAMPAR PROVINSI RIAU Marhasak Stephen H; Roza Elvyra; Yusfiati '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Floodplain river ecosystem in Kampar River of Riau Province has a unique water characteristics. This area was the production center of freshwater fish in Riau. Selais fish was the maskot of Pekanbaru city and typical food from Riau. Selais Kaporeh(Ompok eugeneiatus) is one of fish in Buluh Cina and Mentulik and known to have high economic value in Kampar district. The objectives of this research were to determine the reproduction aspect of Selais Kaporeh including the level of gonads maturity(TKG), index of gonads maturity, fecundity and reproductive potential. This study had been conducted from February to April 2013 in Buluh Cina and Mentulik village. The samples were collected using survey method. The numbers of Selais Kaporeh collected were 155 fishes that consist of 137 females and 18 males, with sex ratio 7,61:1. The length of female fish ranged between 9,3-20,3 cm and weight ranged from 3,1-34,7 g.The length of males range were between 10-14,9 cm and weight range were 2.5-10.8 g. The gonads maturity level of female and male of Selais Kaporeh fish observed rangedfrom TKG I-IV. The index of gonads maturity (IKG) from female and male fish from TKG I-IV were between 0-10,82% and 0-4,4%. IKG increased following TKG and decreased after spawnings. Fecundity were 1825 eggs. Selais Kaporeh did not lay eggs simultaneously in one spawning season and they tend to spawn throughout the year.
ANALISIS ISI LAMBUNG IKAN LAIS JANGGUT (Kryptopterus limpok, Bleeker 1852) DI SUNGAI TAPUNG HILIR PROPINSI RIAU Ravki Adiyanda; Roza elvyra; Yusfiati '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Research on the analysis of stomach content of Lais janggut fish (Kryptopterus limpok, Bleeker 1852) from Tapung River was conducted from December 2012 to February 2013. Fish samples had been taken from the Tapung Hilir river which was divided into three stations: Salembakan Palas, Flamboyan, and Kota Garo. The purposes of this study were to determine the types of food of Lais janggut fish covering the main food, food supplements and additional food at the three stations. The analysis used was the index of preponderance. The index of preponderance was used to analyze the stomach content. It was indicated that the main food and food supplements of Lais janggut fish in Salembakan Palas station consist of Puntius sp (89.3%) and litter (1.1%), arthropods (1.2%), animal debris (8.2%). The Lais janggut fish from Flamboyan station has main food of Puntius sp (84.1%), food supplements in the form of litter (4.5%) and animal debris (8.2%) and additional food such as arthropods (3.2%). Furthermore Lais janggut fish from Kota Garo station has main food debris in the form of animals (45%) and Puntius sp (43.9%), supplementary food such as fish Puntius sp (37%) and food supplements in the form of litter (6.0%) and Arthropods (4.7%). The main food, food supplements, and additional food of Janggut lais fis in Tapung Hilir river were Puntius sp, litter, animal debris, and arthropods respectively.
ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Rizka Suci Akmando; Roza Elvyra; Dewi Indriyani Roslim
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

JOM FMIPA Volume 1 No. 2 Oktober 2014 224 ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Rizka Suci Akmando, Roza Elvyra, Dewi Indriyani RoslimMahasiswa Program S1 Biologi Dosen Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Kampus Bina Widya Pekanbaru, 28293, Indonesia rizkasuciakmando@yahoo.co.idABSTRACT Scientific information of mtDNA D-loop region in fish, Kryptopterus limpok, from Riau is unknown. Before having genetic information, total DNA isolation and amplification is necessary to be carried out. This study was aimed to obtain the total DNA molecules and DNA fragment of the mtDNA D-loop region in fish K. limpok from Sungai Kampar Kiri, Riau. Ten muscle samples were taken from fish collected from the river and used for total DNA isolation. Products of DNA isolation were amplified using the PCR technique with universal primers i.e. L15926 (F) 5’ TCA AAG CTT ACA CCA GTC TTG TAA ACC 3’ dan H00651 (R) 5’ TAA CTG CAG AAG GCT AGG ACC AAA CCT 3’. The PCR process consisted of pre-PCR at 940C for 5 minutes, 35 cycles of PCR consisted of three stages, i.e. as denaturation at 940C for 30 seconds, annealing at 590C for 1 minutes, elongation at 720C for 1 minutes, and post-PCR at 720C for 5 minutes. This study obtained the intact total DNA molecules, with the total DNA concentration of 100 μg/ μl. The length of mtDNA D-loop fragment was 153 bp.