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Tantri Analisawati Sudarsono
Universitas Muhammadiyah Purwokerto
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Perbedaan Kadar Glukosa Serum dan Plasma Naf Segera dan Tunda 2 Jam Pada Penderita DM Retno Sulistiyowati; Budiarti Budiarti; Minto Rahaju; Tantri Analisawati Sudarsono
ULIL ALBAB : Jurnal Ilmiah Multidisiplin Vol. 1 No. 10: September 2022
Publisher : CV. Ulil Albab Corp

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Abstract

Penggunaan sampel serum dan plasma menjadi dua pilihan utama yang sering digunakan untuk mengukur kadar glukosa darah seseorang. Pemeriksaan sampel dengan penundaan perlu ditambahkan bahan pengawet. Natrium Flourida (NaF) merupakan antikoagulan yang dapat mempertahankan konsentrasi glukosa dalam darah jika terjadi penundaan. Tujuan penelitian ini untuk mengetahui efektifitas penggunaan plasma NaF terhadap kadar glukosa darah pada pasien diabetes melitus melalui mekanisme pemeriksaan segera dan ditunda 2 jam. Jenis penelitian ini adalah penelitian observasional analitik dengan menggunakan desain cross sectional. Teknik sampling pada penelitian ini menggunakan Simple Random Sampling (SRS). Hasil penelitian menunjukan nilai α = 0,000 (pemeriksaan segera serum dan plasma NaF) dan nilai α = 0,000 (pemeriksaan ditunda 2 jam serum dan plasma NaF). Terdapat hubungan yang signifikan antara pemeriksaan glukosa pada serum dan plasma NaF segera dan ditunda 2 jam.
Perbedaan Jumlah Trombosit Menggunakan Antikoagulan K3EDTA dengan Volume Sampel Berbeda pada Karyawan Puskesmas Wanadadi 1 Kab. Banjarnegara Wahyu Hidayah; Tantri Analisawati Sudarsono; Linda Wijayanti; Retno Sulistiyowati
ULIL ALBAB : Jurnal Ilmiah Multidisiplin Vol. 1 No. 10: September 2022
Publisher : CV. Ulil Albab Corp

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Platelets (thrombocytes) form a mechanical blockage to the hemostatic response to burns through several reactions, adhesion, aggregation, release, fusion, and procoagulant activity. EDTA prevents platelets from clumping, so it is used as an anticoagulant in the platelet count. Excess concentration of K3EDTA causes shrinkage of blood cells. This study aimed to determine the difference in the number of platelets using K3EDTA anticoagulant with different sample volumes at the Wanadadi 1 Health Center employees, Kab. Banjarnegara. The study was conducted by analytical observation from February August 2022. A total of 10 respondents took 5 ml of blood samples and then divided them into K3EDTA vacutubes with sample volume of 0.5 ml, 1.5 ml, and 2.5 ml. Sample examination using the Hematology Analyzer impedanceelektrik method. The type of research design is analytic observational with a cross-sectional design. Random sampling technique. Data analysis was carried out by conducting a normality test. If the data were normally distributed, p 0.05, then the One Way ANOVA parametric test was continued. The mean number of platelets in a sample volume of 0.5 ml is 287,1000 31,14643 mm, the average number of platelets in a sample volume of 1.5 ml is 294,6000 34.67692 mm, and the average number of platelets in a sample volume of 2.5 ml of 291.1000 35.41955 mm. The lowest mean platelet count was at a volume of 0.5 ml based on statistical tests p = 0.885 (P 0.005). So, there is no significant difference in the number of platelets using K3EDTA Anticoagulants with different sample volumes for employees of Wanadadi Health Care Center 1 Banjarnegara.
Co-Authors Adam Mufarij Ardi Wiranata Arif Mulyanto Budiarti Budiarti Dhanti, Kurnia Ritma Dini Nur Afifah Dita Pratiwi Kusuma Wardani Ermi Susi Yanti Evi Nurhayati Ika Wulan Nuri Anggreani Ismi Andina Pertiwi Lilis Septiana Dewi Linda Wijaya Linda Wijayanti Linda Wijayanti Minto Rahaju Minto Rahaju Nadia Ega Haliza Nur Rizki Aprilia Utami Retno Sulistiyowati Supriyadi Supriyadi Syafira Dian Ergiana Wahyu Hidayah