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Prevalensi Bakteri Methicillin-Resistant Staphylococcus aureus (MRSA) Pada Peralatan Laboratorium kurniawan Kurniawan -; Eva Adaning Tyas; Supriyadi -
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.7554

Abstract

Staphylococcus aureus bacteria are common flora bacteria that are both commensal and opportunistic pathogens in humans. They are commonly treated with antibiotics, which can lead to bacterial resistance, such as the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria, which are resistant to the β -lactam antibiotics that are currently widespread to a variety of communities, such as the campus community, which is equipped with several facilities such as laboratories. The microbiology laboratory is a practicum and research laboratory that uses materials in the form of microorganisms, hence it has the potential to transmit MRSA bacteria. The aim of this study is to find out if MRSA bacteria are present on microbiology lab equipment and how prevalent they are. This study used observational research with a descriptive research design and a purposive sampling strategy. The results of bacterial isolation on MSA medium from fourteen laboratory equipment revealed that thirteen devices exhibited bacterial growth, whereas one device had none. Seven bacterial isolates with S. aureus-like features are detected growing in three of the thirteen equipment, the LAF, incubator, and manual autoclave. The seven bacterial isolates were identified as having round colony shape, glistening, opaque, convex yellow pigmentation, round cell morphology, clustered like grapes and purple in color, positive for coagulase and catalase, and resistance to methicillin antibiotics (MRSA bacteria). It can be concluded that of the fourteen-laboratory equipment examined, three instruments, namely the LAF, incubator, and manual autoclave, were found to be overgrown with MRSA bacteria, with the prevalence of MRSA bacteria in microbiology laboratory equipment reaching 21.4%.  Keywords: laboratory equipment, MRSA, prevalence, S. aureus
Pengaruh Variasi Suhu dan Lama Penyimpanan terhadap Viabilitas Bakteri Asam Laktat (BAL) dan Nilai pH Yoghurt Aditya Dimas Pangestu; Kurniawan Kurniawan; Supriyadi Supriyadi
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2169

Abstract

Consuming yogurt indicates the increasing public awareness of healthy lifestyles has led many people to consume yogurt. Yogurt is a fermented milk product by adding pasteurized skim milk concentrate and adding lactic acid bacteria culture. L. casei is the bacterial culture commonly used in the production of yogurt, which has a role in breaking down lactose into lactic acid. To maintain its quality, yogurt is generally stored at a low temperature. However, there are still many business actors who only store it in a cool box or even at room temperature. The purpose of this study was to describe the viability of LAB and the effect of variations in temperature and storage time on the viability of LAB and pH value of yogurt. This study used an experimental method with a completely randomized design (CRD) with 3 treatments in the form of variations in storage temperature, namely 5°C, 15°C, and 25°C, each treatment temperature was stored for 1 week, 2 weeks, 3 weeks, and 4 weeks. This study was conducted in December 2020. The results showed that the highest viability value (289x106 CFU/mL) of lactic acid bacteria in yogurt was obtained at 5°C with two weeks of storage. Yogurt's pH value is influenced by temperature and storage time; the higher the temperature and storage time, the causes the yogurt's pH value decreases. The conclusion that can be drawn from this study is that the highest viability of LAB was obtained at 5°C with a shelf life of two weeks, while the best pH value of yogurt was achieved at 5°C for one week.
IDENTIFIKASI JAMUR PATOGEN PENYEBAB DERMATOFITOSIS PADA JARI KAKI PETANI DI DESA BOJONGSARI, BANYUMAS. Afriska Nurhidayah; Kurnia Ritma Dhanti; Supriyadi Supriyadi
Jurnal Labora Medika Vol 5, No 1 (2021): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.337 KB) | DOI: 10.26714/jlabmed.5.1.2021.8-17

Abstract

Dermatofitosis merupakan penyakit yang disebabkan oleh kolonisasi jamur dermatofita pada jaringan yang mengandung keratin seperti stratum korneum kulit, rambut dan kuku pada manusia. Dermatofitosis tersebar di seluruh dunia dengan prevalensi yang berbeda-beda tiap negara dan Indonesia menempati urutan kedua. Tingginya kasus tersebut didukung dengan Indonesia merupakan salah satu negara beriklim tropis yang memiliki suhu dan kelembaban tinggi, dimana merupakan suasana yang baik bagi pertumbuhan jamur, higiene juga berperan untuk timbulnya penyakit ini, sehingga jamur dapat ditemukan hampir di semua tempat. Tujuan dari penelitian ini adalah untuk mengetahui adanya jamur patogen penyebab dermatofitosis pada kerokan kulit sela-sela jari kaki petani di Desa Bojongsari, Kecamatan Kembaran, Kabupaten Banyumas. Metode penelitian yang digunakan adalah metode deskriptif dengan teknik pengambilan sampel purposive sampling dengan jumlah sampel 8 petani. Pengambilan sampel uji berupa kerokan kulit sela-sela jari kaki petani yang menunjukkan karakteristik dermatofitosis. Berdasarkan hasil penelitian secara makroskopis (warna koloni, warna balik koloni, hifa udara, tekstur permukaan, tepi koloni, pola koloni dan diameter koloni) sedangkan pengamatan mikroskopis (spora dan hifa) menunjukkan adanya jamur Aspergillus sp. (2 isolat), Penicillium sp. (7 isolat) dan Trichophyton sp. (1 isolat). Kesimpulan dari penelitian ini, dari 8 sampel diperoleh 10 isolat jamur patogen meliputi jamur Aspergillus sp., Penicillium sp. dan Trichophyton sp.
Effectiveness of ginger and cinnamon compresses on pain in elderly with osteoarthritis Nur Fitriani; Supriyadi Supriyadi
Proceedings Series on Health & Medical Sciences Vol. 1 (2020): Proceedings of the 1st International Conference of Health Science Faculty Universitas
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.362 KB) | DOI: 10.30595/pshms.v1i.26

Abstract

Osteoarthritis (OA) was one of the most common chronic diseases and a major cause of pain and disability that decreased health status. This disease caused pain and disability in patients which disturbed daily activities. Warm compress is one of the non-pharmacological therapies used to reduce pain. Warm compress therapy is applied at the sub-acute and chronic stage of osteoarthritis to reduce pain, compression and pain in the joints. Warm compresses can be combined by adding ginger and cinnamon. The aim of the reaesarh to understand the effectiveness of ginger and cinnamon compresses on lowering pain in the elderly with osteoarthritis in the orphanage of Sudagaran. This research was quasi-experimental design with two pretest-posttest design groups was used in this study. The samples were collected by using a random sampling technique with a total of 15 respondents getting ginger compress and 15 respondents getting cinnamon compress which was in accordance with the inclusion and exclusion criteria. The data were analyzed by using univariate and bivariate with Mann Whitney U Test. The results of the Mann Withney U Test on the second post-therapy obtained the difference in the value of ginger compresses with p = 0.417 and the difference in comfort value of cinnamon compresses with p = 0.127. After calculating the size effect, the ginger size effect was 0.924 while the cinnamon size effect was 0.790. The use of ginger compresses is more effective than cinnamon compresses in lowering pain in the elderly with osteoarthritis in the Community Health Center of Sudagaran.
Relationship of nutritional completeness with events of stunting in children age 1- 2 years Renaldi Gusela Wilian Nanda; Supriyadi
Proceedings Series on Health & Medical Sciences Vol. 1 (2020): Proceedings of the 1st International Conference of Health Science Faculty Universitas
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.185 KB) | DOI: 10.30595/pshms.v1i.32

Abstract

The problem of stunting is one of the nutritional problems that the world face, especially in poor and developing countries. Stunting is a chroniac malnutrition in the period of growth and development since early life. Nutrition is an important element in the fulfilment of nutritional needs because it serves to produce energy, build and maintain tissues, and regulate life processes in the body. In addition, nutrition is related to brain development, learning ability and work productivity. Stunting or short is a condition of failure to thrive in infants (0-11 months) and toddlers (12-59 months) as a result of chronic malnutrition, especially in the first 1,000 days of life so that the child is too short for their age. The objective is to determine the relation between the completeness of nutritional stats and the incidence of stunting in children aged of 1-2 years in the working area of Community Health Center I Kembaran. This type of research was quantitative using a descriptive correlation design with a cross sectional approach. The population in this study were children under 2 years who met the criteria. The sampling technique used was simple random sampling, namely 86 respondents were included. The analysis used was the chi square statistical test. Statistics in this study consisted of frequency of completeness of nutritional status with the occurrence of stunting in children aged 1-2 years. From the results of data processing, it was found that there was a relationship between the completeness of nutritional status with the incidence of stunting in children aged 1-2 years in the working area of Community Health Center I Kembaran with a p-value of 0,000 <0.005. There was a relationship between the completeness of nutritional status with the incidence of stunting in children aged 1-2 years in the working area of Community Health Center I Kembaran.
PELATIHAN DETEKSI DINI STROKE DENGAN METODE SEGERA KE RS BAGI WARGA MUHAMMADIYAH Sodikin Sodikin; Asiandi Asiandi; Supriyadi Supriyadi
PROSIDING SEMINAR NASIONAL LPPM UMP Vol 2 (2020): PROSIDING SEMINAR NASIONAL LPPM UMP 2020
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (523.639 KB)

Abstract

Tujuan: kegiatan ini merupakan salah satu upaya promotif serta preventif dalam menurunkan prevalensi stroke. Tujuan kegiatan ini adalah meningkatkan pengetahuan dan kemampuan melakukan deteksi dini dari serangan penyakit sroke. Metode: Program ipteks bagi masyarakat ini dilakukan dengan metode ceramah, diskusi dan tanya jawabsimulasi/demonstrasi terkait dengan cara melakukan deteksi dini stroke. Alat evaluasi keberhasilan program ini dilakukan dengan melihat skor pengetahuan dan kemampuan detesksi dini stroke ”SEGERA KE RS” pre dan post test dari edukasi dan pelatihan. Hasil: Berdasar uji normalitas menggunakan uji Kolmogorov-Smirnov menunjukkan variabel pengetahuan tentang stroke berdistribusi normal, p = 0,173 sehingga analisis menggunakan uji t paired. Sedangkan variabel pengetahuan tentang deteksi dini stroke menggunakan slogan ”SEGERA KE RS” tidak berdistribusi normal, p <0,001 sehingga analisis menggunakan uji Wilcoxon Signed Ranks. Berdasarkan hasil program ipteks bagi masyarakat menunjukkan skor rata-rata pengetahuan peserta IbM tentang stroke sebelum pelatihan adalah 89 (dengan skor minimum-maksimum adalah 80-100) dan setelah pelatihan adalah 93,50 (dengan skor minimum-maksimum adalah 70-100). Skor rata-rata pengetahuan tentang deteksi dini stroke dengan slogan ”SEGERA KE RS” sebelum pelatihan adalah 73,20 (dengan skor minimum-maksimum adalah 0-100) dan sesudah pelatihan adalah 96,85 (dengan skor maksimum-minimum adalah 67100). Sedangkan berdasarkan analisis menunjukkan tidak adanya perbedaan yang signifikan pada pengetahuan tentang stroke antara sebelum dan sesudah pelatihan, t(df) = -1,917(19), p = 0,070, r = 0,40; untuk pengetahuan tentang deteksi dini dengan menggunakan slogan ”SEGERA KE RS” menunjukkan ada perbedaan yang si gnifikan antara sebelum dan sesudah pelatihan, z = -2,238, p = 0,025, r = -0,50. Kesimpulan: berdasarkan analisis statistik menunjukkan tidak ada perbedaan yang signifikan pengetahuan tentang deteksi dini stroke dengan slogan ”SEGERA KE RS” sebelum dan sesudah pelatihan.
Hubungan Tingkat Pengetahuan, Motivasi, dan Behaviour Skill Model dengan Perilaku Pemeriksaan Payudara Sendiri (SADARI) pada Mahasiswi Keperawatan S1 Universitas Muhammadiyah Purwokerto Pratiwi Ayuningtyas; Supriyadi Supriyadi
Proceedings Series on Health & Medical Sciences Vol. 4 (2023): Proceedings of the Midwifery Conference on Collaborative Maternity Care (DYNAMIC)
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pshms.v4i.569

Abstract

Latar Belakang: Pemeriksaan payudara sendiri (SADARI) merupakan metode deteksi dini kanker payudara pada wanita yang dilakukan sedini mungkin. Di Indonesia, kanker payudara merupakan penyakit tertinggi dengan prevalensi sebesar 16,6% (65.858) kasus.Tujuan: Mengetahui hubungan tingkat pengetahuan, motivasi, dan behaviour skills model dengan perilaku pemeriksaan payudara sendiri (SADARI) pada mahasiswi Keperawatan S1 di Universitas Muhammadiyah Purwokerto.Metode: Penelitian ini merupakan penelitian kuantitatif dengan pendekatan cross sectional. Sampel penelitian ini adalah mahasiswi Keperawatan S1 semester 4 dengan jumlah 58 responden dengan metode simple random sampling pada bulan Januari-Februari 2022. Instrumen penelitian ini menggunakan lembar kuesioner dengan uji Chi-Square. Hasil: Hasil penelitian didapatkan 34 responden (58,6%) perilaku SADARI buruk, 55,2% mahasiswi dengan tingkat pengetahuan rendah, 53,4% mahasiswi dengan motivasi rendah, dan 55,2% mahasiswi dengan behaviour skills model tidak terampil. Ada hubungan tingkat pengetahuan dengan perilaku SADARI (p-value= 0,000), ada hubungan motivasi dengan perilaku SADARI (p-value= 0,002), dan ada hubungan behaviour skills model dengan perilaku SADARI (p-value= 0,001).Kesimpulan: Ada hubungan antara tingkat pengetahuan, motivasi, dan behaviour skills model dengan perilaku SADARI.
Hubungan Kepemilikan Jamban dengan Kejadian Diare di Desa Bagan Laguh, Kecamatan Bunut, Riau: The Correlation Between Toilet Ownership With the Event of Diarrhea in Bagan Laguh Village, Bunut Sub-District, Riau Nur Rizki Aprilia Utami; Tantri Analisawati Sudarsono; Retno Sulistiyowati; Supriyadi Supriyadi
Jurnal Surya Medika (JSM) Vol. 9 No. 1 (2023): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v9i1.5164

Abstract

Diarrhea is an endemic disease that can cause Extraordinary Events (KLB). In 2017, there were 21 occurrences of diarrhea spreading across 12 provinces with a total of 1,725 patients and 34 deaths (CFR 1.97%). There are three risk factors for diarrhea, namely individual characteristics, preventive behavior, and environment. This research is to determine the correlation between toilet ownership and the occurrence of diarrhea in Bagan Laguh Village, Riau. The research population was all residents of Bagan Laguh Village listedin the Integrated Social Welfare Data (DTKS) and 59 residents were selected as the research sample using the simple random sampling technique. The primary data were obtained from the questionnaires filled out by the respondents and then analyzed using the Chi-Square test. The results of this research indicated that respondents' level of education and income are not significantly correlated with the occurrence of diarrhea(P values= 0.115 and 0.421 respectively); however, respondents' knowledge, attitude, and toilet ownership are correlated with the occurrence of diarrhea significantly (P-values = 0.007, 0.001, and 0.002 respectively).
Menumbuhkan Kreatifitas Berwirausaha Di Kalangan Angkatan Muda Muhammadiyah Ragil Setiyabudi; Isna Hikmawati; Supriyadi Supriyadi
JURNAL PENGABDIAN AL-IKHLAS UNIVERSITAS ISLAM KALIMANTAN MUHAMMAD ARSYAD AL BANJARY Vol 9, No 2 (2023): AL-IKHLAS JURNAL PENGABDIAN
Publisher : Universitas Islam kalimantan MAB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/jpaiuniska.v9i2.11898

Abstract

Ikatan Mahasiswa Muhammadiyah sebagai bagian dari Angkatan Muda Muhammadiyah tidak hanya bergerak dalam bidang dakwah keagamaan, pengembangan lingkungan dan dakwah sosial saja akan tetapi juga terdapat  atau pengembangan nilai karakter diri sendiri, penanaman nilai nasionalisme dan kegiatan kewirausahaan.Oleh karena itu diperlukan kegiatan dalam bentuk ceramah interaktif, tanya jawab, dan workshop atau pendampingan atau coaching pembuatan perencanaan bisnis dan evaluasi untuk membentuk karakter wirausaha. Hasil kegiatan menunjukkan peningkatan skor pemahaman tentang kewirausahaan antara sebelum dengan sesudah melakukan ceramah dan tanya jawab sebesar 13,4 poin mengindikasikan evaluasi ceramah dan tanya jawab berjalan dengan baik. Peningkatan skor tersebut berarti menggambarkan peningkatan tentang pemahaman tentang sikap mental wirausahawan, mencari ide dan peluang bisnis, permodalan, penyiapan alat dan bahan produksi, proses produksi, pengemasan, pemasaran dan evaluasi. Hasil pendampingan atau coaching menunjukkan ide wirausaha muncul dari masing-masing kelompok yang kemudian menghasilkan perencanaan bisnis.
The Effects of Family Function, Nurse-Patient Communication, and Stigma on Depression Symptoms in Tuberculosis Patients at the DOTS Pulmonary Outpatient Clinic of RSUD Banyumas Febri Restu Ristiyanti; Supriyadi Supriyadi
Proceedings Series on Health & Medical Sciences Vol. 5 (2024): Proceedings of the 4th International Nursing and Health Sciences Universitas Muhammad
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pshms.v5i.958

Abstract

Background: Tuberculosis (TB) and mental illness are prevalent worldwide and often coexist. Poor mental health has been linked to alterations in immune function. Through social, behavioral, and biological mechanisms, depression and tuberculosis together amplify the burden of disease. Collaboration from all parties is needed to alleviate depression in TB patients, including support from family function and positive societal stigma. Method: This was a descriptive correlational study, and data collection was carried out using a cross-sectional method. A total of 72 respondents who met the inclusion and exclusion criteria were selected as the sample using a non-probability sampling technique, specifically accidental sampling. Results: The results of the multiple regression test showed that only the variables of family function and stigma had a significant correlation with p-values of 0.00005 and 0.009 0.05, respectively. Meanwhile, the variable of nurse-patient communication had a p-value of 0.633 0.05, indicating no significant correlation. Additionally, the calculated F-value was 18.662, and the tabled F-value was 3.136, with a probability value of 0.000. Since the probability value was less than 0.05, the regression model could be used to predict depression symptoms. It can be stated that the three independent variables, namely family function, nurse-patient communication, and stigma, collectively influence depression symptoms in patients. Conclusion: There is a significant relationship between family function and stigma towards depression symptoms in Tuberculosis patients at the DOTS Pulmonary Clinic of Banyumas Regional Public Hospital in 2023.