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All Journal Indonesian Journal of Pharmaceutical Science and Technology As-syifaa Jurnal Farmasi FITOFARMAKA: Jurnal Ilmiah Farmasi Jurnal Farmamedika (Pharmamedica Journal) Jurnal Medika Cendikia (e-Journal) Jurnal Ilmiah Farmako Bahari Indonesian Journal of Community Empowerment
Siva Hamdani
Program Studi Farmasi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Garut, Garut
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Inhibitory Activity of Polypodium feei Root Extracts on Nitric Oxide and TNF-α Synthesis in LPS‐activated RAW264.7 Cell Line Suwandi, Deden Winda; Hamdani, Siva; Ruchiyat, Ruchiyat
Indonesian Journal of Pharmaceutical Science and Technology Vol 12 (2025): Vol. 12 Suppl. 2 (2025)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v12i0.59351

Abstract

Polypodium feei (P. feei) roots have been reported to contain sellegueain A, a compound with analgesic and anti-inflammatory properties demonstrated in experimental animals. However, the anti-inflammatory activity of extracts, which represent the form most used in traditional medicine, has not been scientifically validated. This study aims to test the anti-inflammatory activity of P. feei root extract in inhibiting the synthesis of inflammatory mediators such as NO, iNOS, and TNF-α in RAW264.7 macrophage cells stimulated by lipopolysaccharide (LPS). RAW264.7 cells were used to evaluate the inhibitory activity of test compounds on the production of inflammatory mediators NO, iNOS, and TNF-α. Research parameters included cell viability testing with MTS, colorimetric test to determine the concentration of nitric oxide (NO), ELISA test to measure protein levels of the iNOS and TNF-α. P. feei root extract at concentrations of 10, 50, 100, and 150 µg/mL inhibited the production of inflammatory mediators with the best activity in inhibiting NO and iNOS with IC50 values of 87.22 and 99.99 µg/mL, respectively. This study shows that P. feei root extract has anti-inflammatory activity through inhibiting the production of NO, iNOS and TNF-α mediators in LPS-stimulated RAW264.7 macrophage cells.
Co-Authors Aan Novianti Aji Najihudin Alfani, Yustika Auliasari, Nurul Azizah, Winanda Nur Fajar Fauzi Abdullah Fajar Firdaus, Muhammad Framesti Frisma Sriarumtias Gazzali, Amirah Mohd Genialita Fadhilla Hesti Renggana Hindun, Siti Hindun, Siti Mariyam, Imas Siti Muhammad Akbar Najihudin, Aji Nuari, Doni Anshar Oktavianti, Suci Paula Khofifah RANTIKA, NOPI Renggana, Hesti Ruchiyat, Ruchiyat Sagita Apionita Santi Nurhasanah Suwandi, Deden Suwandi, Deden Winda Tia Rahayu Yustika Alfani