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Extraction and Characterization of Urease from Durio zibethinus L Zusfahair Zusfahair; Amin Fatoni; Dian Riana Ningsih; Mardiyah Kurniasih
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 7, No. 2, November 2021
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v7i2.21768

Abstract

Urease is a biocatalyst that serves to hydrolyze urea into ammonia and carbon dioxide. Since it is an imported product, the price of urea is still high. Urease can be found in grains. One of the grains that has not been explored for its urease content is durian (Durio zibethinus L.) seeds. This study aims to determine the effect of germination time on the activity of urease from durian seeds and its characteristics including the effect of pH, incubation temperature, enzymatic reaction time, addition of EDTA and metals, and storage time on the activity of urease from durian seeds. The first step of this study was seed germination which was carried out in the dark for 0, 3, 5, 7, and 9 days. Durian seed sprouts were extracted by mashing them using a mortar and pestle. They were then homogenized using a stirrer and centrifuged in a cold state. The crude urease extract obtained was then tested for its activity using the Nessler method. The acquired data was tested statistically using ANOVA. The results showed that the activity of urease from durian seeds was optimum at 3-day germination time, pH 7, incubation temperature at 30 °C, and 15-minute enzymatic reaction time with an activity of 163.6 U/mL. Urease is a metalloenzyme with its inhibitor being the Cu2+ and Na+ metal ions and its activator being Ba2+ metal ion. Tukey's test analysis showed that the effect of urease storage time at 4 °C resulted in a stable urease activity for 8 days while at room temperature it decreased its activity significantly to 72.8%.
PEMANFAATAN PROTEASE DARI KULIT NANAS (Ananas comosus, L) DALAM DEGUMMING BENANG SUTERA Zusfahair Zusfahair; Amin Fatoni; Dian Riana Ningsih
Jurnal Kimia Riset Vol. 1 No. 1 (2016): Juni
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (813.159 KB) | DOI: 10.20473/jkr.v1i1.2438

Abstract

AbstrakProtease dalam bidang industri tekstil dapat berperan pada proses degumming benang sutera. Salah satu protease yang dapat digunakan untuk degumming benang sutera dapat diisolasi dari kulit nanas. Penelitian ini bertujuan untuk mengetahui potensi protease dari kulit nanas dalam proses degumming benang sutera. Isolasi protease dari kulit nanas dilakukan dengan ekstraksi menggunakan buffer fosfat dilanjutkan dengan sentrifugasi untuk memisahkan debrisnya. Ekstrak protease dari kulit nanas selanjutnya digunakan dalam proses degumming benang sutera pada suhu dan waktu perendaman tertentu. Benang sutera hasil proses degumming diamati secara visual, menggunakan mikroskop cahaya dan mikroskop electron (SEM). Hasil penelitian menunjukkan bahwa proses degumming benang sutera yang optimal dilakukan pada suhu 50 °C, dan waktu inkubasi selama 4 jam. Benang sutera yang dihasilkan dengan protease ini lebih lembut dan berkilau, jika dibandingkan dengan benang sutera yang diolah secara tradisional menggunakan sabun dan pemanasan. Kata kunci: degumming, protease, kulit nanas, sutera  AbstractProtease could be used in the textile industry for degumming of silk fabric. One of prospective proteases for silk degumming that of from a pineapple peels. This study was performed to determine the potential of protease from pineapple peels for silk degumming. The protease was extracted from pineapple peels using phosphate buffer, continued by separating the crude protease from the debris using centrifugation. The crude protease was then used to degum the raw silk, by soaking it in certain of incubation time and temperature. Silk degumming results observed visually, also by light microscope and electron microscope. The results showed that the optimum degumming process is at 50 ° C incubation for 4 hours. In comparison to the traditional silk degumming using soap and heating, the protease based degumming showed a softer and shiny silk fabric. Keyword:  degumming, protease, pineapple peel, silk.
QUALITATIVE ANALYSIS METHOD OF DETECTION OF WAX CONTENT IN GORENGAN USING SMARTPHONE Yulia Yulia; Hendri Wasito; Amin Fatoni
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 15, No 1 (2018)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.849 KB) | DOI: 10.24071/jpsc.001052

Abstract

Wax is one of the compounds that can be misused to be added to Gorengan, Indonesian fritter, to keep them crispy. Gorengan containing wax is difficult to identify visually, so a quick and easy method of detecting wax content is required. The purpose of this research is to develop and evaluate the analytical performance of detecting wax content in gorengan using smartphone. Gorengan sample was dissolved with hexane and then added reagent that will give discoloration followed by analysis using smartphone. Some analysis performance parameters were evaluated in terms of linearity and detection limit, qualitative analysis capability, precision, and selectivity test. The developed method was also applied in some gorengan samples. The result shows that the detection of wax content in gorengan can be conducted by using reagent consisting of NaOH, Schift, and curcumin (1 : 2 : 2). Performance analysis shows that the linearity measurement at concentration between 10% and 25% has correlation coefficient (r) of 0.9537 with detection limit at concentration of 2% and precision (%RSD) less than 3%. The developed method can be applied for the detection of wax content in gorengan in the market.
Backmatter (Instruction for author and back cover) Amin Fatoni
Molekul Vol 15, No 1 (2020)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.635 KB) | DOI: 10.20884/1.jm.2020.15.1.652

Abstract

Backmatter (Instruction for author and back cover)
Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents) Amin Fatoni
Molekul Vol 15, No 2 (2020)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.299 KB) | DOI: 10.20884/1.jm.2020.15.2.690

Abstract

Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents) of the print version
Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents) Amin Fatoni
Molekul Vol 15, No 3 (2020)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.392 KB) | DOI: 10.20884/1.jm.2020.15.3.742

Abstract

Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents)
Backmatter (Instruction for author and back cover) Amin Fatoni
Molekul Vol 14, No 2 (2019)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.309 KB) | DOI: 10.20884/1.jm.2019.14.2.617

Abstract

No Abstract
Backmatter (Instruction for author and back cover) Amin Fatoni
Molekul Vol 16, No 1 (2021)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.172 KB) | DOI: 10.20884/1.jm.2021.16.1.770

Abstract

Backmatter (Instruction for author and back cover)
Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents) Amin Fatoni
Molekul Vol 16, No 2 (2021)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.679 KB) | DOI: 10.20884/1.jm.2021.16.2.806

Abstract

Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents)
Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents) Amin Fatoni
Molekul Vol 16, No 3 (2021)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.199 KB) | DOI: 10.20884/1.jm.2021.16.3.875

Abstract

Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents)