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AMOBILISASI PROTEASE DARI Bacillus sp. BT 1 MENGGUNAKAN POLIAKRILAMIDA PROTEASE IMMOBILIZATION FROM Bacillus sp. BT 1 USING POLYACRYLAMIDE Zusfahair Zusfahair; Amin Fatoni
Molekul Vol 6, No 2 (2011)
Publisher : Universitas Jenderal Soedirman

Penggunaan protease pada umumnya, dalam bentuk enzim bebas yang hanya sekali pakai, sehingga biaya produksi yang melibatkan enzim ini menjadi mahal. Amobilisasi enzim dapat mengatasi masalah ini, yang memungkinkan penggunaan enzim berulang kali. Dalam penelitian ini, protease dari Bacillus sp. BT 1, yang diperoleh dari sumber air panas, diamobilisasi dengan jebakan menggunakan poliakrilamida. Ekstrak kasar dalam bentuk enzim protease bebas dan enzim amobil dikarakterisasi termasuk suhu optimum, pH optimum, waktu inkubasi dan stabilitas enzim amobil pada penggunaan berulang. Aktivitas protease diukur dengan menggunakan metode Kunitz yang modifikasi. Hasil penelitian menunjukkan waktu produksi optimum protease adalah 36 jam yang berada pada akhir fase eksponensial pertumbuhan bakteri. Amobilisasi ekstrak kasar protease Bacillus sp BT 1 dapat menjebak 47,18% dari protease. Suhu optimum protease bebas 60 oC dan meningkat menjadi 70 oC pada penggunaan protease amobil. Protease bebas dan protease amobil memiliki pH optimum yang sama yaitu 11. Protease amobil tidak kehilangan aktivitas secara signifikan sampai empat kali penggunaan.

Chitosan–Fe3O4 Nanoparticles Cryogel for Glucose Biosensor Development Amin Fatoni; Vonia Febriana Hidayah; Suyata Suyata; Hartiwi Diastuti; Mekar Dwi Anggraeni
Science and Technology Indonesia Vol. 8 No. 1 (2023): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2023.8.1.52-58

Chitosan was widely used as a supporting material for enzyme immobilization. However, the non-conductive properties of chitosan could be a severe problem in the application of biosensors with electrochemical detection. This research aimed to modify the chitosan cryogel with Fe3O4 nanoparticles for glucose biosensor application. The glucose biosensor used glucose oxidase enzyme as biological sensing element which was immobilized on the working electrode of electrochemical detection. Chitosan-Fe3O4 composite cryogel was used as supporting material for glucose oxidase immobilization. The detection optimization was also performed by varying the operating conditions such as buffer pH and reaction temperature. The result showed the optimum conditions were the addition of Fe3O4 nanoparticles for 4% (w/v), phosphate buffer solution of 100 mM with pH of 7.0, and reaction temperature at 25°C. The glucose determination showed linearity for increasing oxidation peak and decreasing reduction peak with the glucose concentration, with regression equation of y = -6.804x – 104.32 and y = 4.5872x + 133.37 respectively. Furthermore, the limit of detection and limit of quantification for oxidation peaks were 0.38 mM and 1.25 mM respectively. The reduction peak showed a limit of detection of 0.32 mM and a limit of quantification of 1.07 mM.

Immobilization of urease from Phaseolus vulgaris L. seeds using calcium alginate as a support matrix ZUSFAHAIR ZUSFAHAIR; DIAN RIANA NINGSIH; AMIN FATONI; ELY SETIAWAN
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Urease is an enzyme that functions as a catalyst in the hydrolysis of urea into ammonia and carbon dioxide. The industrial sector has made extensive use of urease. To date, enzymes are used in free form, deemed less effective. Therefore, enzymes are used in immobilized form because they can be utilized repeatedly. This research aimed to isolate urease from kidney bean (Phaseolus vulgaris L.) seed and immobilize it using a Ca-alginate support matrix and a trapping technique. Eight days were devoted to germinating kidney bean seeds to begin the investigation. Isolation of crude urease extract from kidney beans was carried out using phosphate buffer pH 7. It was then immobilized with Ca-alginate at different concentrations of Na-alginate and contact times The crude free and immobilized urease extract was further characterized including pH, temperature and stability of repeated use. The urease activity was determined using the Nessler method using a spectrophotometer. The results demonstrated that urease immobilization from kidney bean seeds with a Ca-alginate matrix was most effective at a concentration of 5% Na-alginate and a contact period of 60 minutes, yielding a value of 5.92 U/mL. The optimal pH of free and immobilized urease was 7 and 8, respectively, and temperatures of 35 and 40 °C, respectively. The immobilization of urease from kidney bean seeds using a Ca-alginate support matrix increased the stability of recurrent use by fivefold, while the relative urease activity remained at 52%.

IbM Pembentukan Posyandu Lansia Mekar Dwi Anggraeni; Amin Fatoni; Warsinah Warsinah
Darma Sabha Cendekia Vol 1 No 1 (2019)
Publisher : Pasca Sarjana | Universitas Jenderal Soedirman

Pengabdian Kepada Masyarakat (PKM) yang telah dilakukan berupa pembentukan dan pelaksanaan Posyandu Lansia di RT 1 dan 3 RW 1 dan RW 4 Kelurahan Bancar Kembar Kecamatan Purwokerto Utara Kabupaten Banyumas. Pelaksanaan pengabdian selama 8 bulan yaitu bulan April sampai dengan November 2017. Tujuan dilaksanakannya PKM ini adalah pemberdayaan masyarakat terkait dengan Posyandu Lansia meliputi: Pembentukan Posyandu Lansia, Pelatihan Kader Posyandu, Pelatihan Senam Lansia, Pelatihan cek kadar glukosa, kolesterol, asam urat darah, siraman rohani, serta Pelatihan cara mengukur tekanan darah. Hasil dari kegiatan PKM menunjukkan anggota masyarakat sangat bersemangat dengan adanya pembentukan dan pelaksanaan Posyandu Lansia. Hal ini disebabkan karena kader Posyandu Lansia mendapatkan berbagai pelatihan dan peralatan yang menunjang pelaksanaan Posyandu lansia. Para lansia juga bersemangat mengikuti berbagai aktivitas pada saat Posyandu Lansia yaitu senam lansia, penimbangan berat badan, pengukuran berat badan, pengukuran tekanan darah, deteksi dini kadar kolesterol, asam urat dan gula darah, siraman rohani, pendidikan kesehatan tentang pola hidup sehat, pemberian makanan tambahan, dan manajemen penyakit kronis. Posyandu lansia merupakan salah satu wadah bagi upaya pencegahan penyakit dan promosi kesehatan bagi lansia sehingga dapat menekan biaya pengobatan penyakit yang dikeluarkan oleh masyarakat dan negara

Pengenalan Elektrokimia untuk Analisis Vitamin C bagi Siswa SMA Amin Fatoni; Zusfahair Zusfahair; Dadan Hermawan
Darma Sabha Cendekia Vol 2 No 2 (2020): Darma Sabha Cendekia - Desember 2020
Publisher : Pasca Sarjana | Universitas Jenderal Soedirman

Mata pelajaran kimia telah dikenalkan ke siswa Sekolah Menengah Atas (SMA) maupun Universitas, namun demikian, tidak semua teori kimia tersebut dipraktekkan di laboratorium karena berbagai keterbatasan. Metode analisis kimia merupakan salah satu topik dalam mata pelajaran kimia yang diaplikasikan secara luas pada bidang kesehatan. Salah satu metode kimia yang menarik dan banyak aplikasinya adalah metode elektrokimia dalam mendeteksi suatu senyawa. Metode ini jarang dilakukan praktek langsung karena keterbatasan instrumen yang mahal. Kegiatan pengabdian kepada masyarakat bertujuan untuk mengenalkan siswa SMA teori dan praktek metode analisis kimia menggunakan instrumen potentiostat untuk analisis senyawa elektroaktif, misalnya kadar vitamin C. Kegiatan ini dilaksanakan dalam suasana pandemi Covid-19, sehingga jumlah siswa yang terlibat dibatasi yaitu 15 siswa setiap hari, dan dilaksanakan selama 2 hari. Hasil pengabdian kepada masyarakat menunjukkan peningkatan pengetahuan dan keterampilan dari para siswa yang dilatih.

Pelatihan Uji Formalin Menggunakan Smartphone dan Scanner untuk Siswa SMA Amin Fatoni; Mekar Dwi Anggraeni; Anung Riapanitra; Zusfahair Zusfahair
Darma Sabha Cendekia Vol 3 No 3 (2021): Darma Sabha Cendekia - November 2021
Publisher : Pasca Sarjana | Universitas Jenderal Soedirman

Sekolah Menengah Atas (SMA) sudah mengajarkan pembelajaran kimia, namun demikian, belum semua melaksanakan praktikum untuk lebih memahami ilmu kimia, karena keterbatasan saran terutama alat dan petunjuk praktikumnya. Keterbatasan instrumentasi yang mahal memicu para peneliti untuk memanfaatkan alat-alat sederhana disekitar kita seperti kamera smartphone, kamera poket, kamera DSLR dan alat pemindai dokumen (scanner) sebagai alternatif instrumentasi kimia. Kegiatan Pengabdian masyarakat ini dikenalkan teori untuk meningkatkan pengetahuan siswa dan praktik untuk meningkatkan keterampilan siswa tentang penggunaan kamera smartphone dan scanner sebagai alat analisis kadar formalin. Hasil kegiatan pengabdian kepada masyarakat menunjukkan pemberian materi menunjukkan peningkatan pengetahuan dari skor rata-rata 68 menjadi 80, sedangkan praktikum dikelas memberikan peningkatan pengetahuan alat, bahan dan prosedur analisis kimia menggunakan smartphonedan scanner dari skor rata-rata 54,7 menjadi 86

Chiral Separation of Econazole by High Performance Liquid Chromatography Method using Cyclodextrin as Chiral Column Dadan Hermawan; Cacu Cacu; Khansa Salsabila; Suwandri Suwandri; Amin Fatoni; Uyi Sulaeman; Ponco Iswanto; Mudasir Mudasir; Hassan Y. Aboul-Enein
Molekul Vol 17 No 2 (2022)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2022.17.2.6348

The chiral separation of econazole, an antifungal drug with one chiral center has been successfully carried out using the high-performance liquid chromatography (HPLC) method. Enantioresolution of econazole (Rs = 2.29) was achieved using cyclodextrin-based chiral column (Astec Cyclobond, 25 cm × 4.6 mm × 5 μm), mobile phase composition of acetonitrile : water (0.2% HCOOH) (20:80, v/v), and UV detection of 220 nm.The optimized HPLC method has been applied for the quantitative determination of econazole in the pharmaceutical (liquid) sample withpercentage recovery of 100.75 % (RSD = 0,95%; n = 3). The effect of several HPLC parameters on the chiral separation of econazole was also evaluated and the method was successfully validated in terms of linearity, accuracy, precision, and selectivity. The present HPLC method was simple, short analysis time, and high resolution.

Immobilization of urease from Phaseolus vulgaris L. seeds using calcium alginate as a support matrix ZUSFAHAIR ZUSFAHAIR; DIAN RIANA NINGSIH; AMIN FATONI; ELY SETIAWAN
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i3.26056

Urease is an enzyme that functions as a catalyst in the hydrolysis of urea into ammonia and carbon dioxide. The industrial sector has made extensive use of urease. To date, enzymes are used in free form, deemed less effective. Therefore, enzymes are used in immobilized form because they can be utilized repeatedly. This research aimed to isolate urease from kidney bean (Phaseolus vulgaris L.) seed and immobilize it using a Ca-alginate support matrix and a trapping technique. Eight days were devoted to germinating kidney bean seeds to begin the investigation. Isolation of crude urease extract from kidney beans was carried out using phosphate buffer pH 7. It was then immobilized with Ca-alginate at different concentrations of Na-alginate and contact times The crude free and immobilized urease extract was further characterized including pH, temperature and stability of repeated use. The urease activity was determined using the Nessler method using a spectrophotometer. The results demonstrated that urease immobilization from kidney bean seeds with a Ca-alginate matrix was most effective at a concentration of 5% Na-alginate and a contact period of 60 minutes, yielding a value of 5.92 U/mL. The optimal pH of free and immobilized urease was 7 and 8, respectively, and temperatures of 35 and 40 °C, respectively. The immobilization of urease from kidney bean seeds using a Ca-alginate support matrix increased the stability of recurrent use by fivefold, while the relative urease activity remained at 52%.

The Isolation, Immobilization, and Characterization of Urease from The Seeds of Winged Bean (Psophocarpus tetragonolobus (L.) DC. Zusfahair, Zusfahair; Ningsih, Dian Riana; Fatoni, Amin; Bilalodin, Bilalodin; Nuraini, Aprilia Nafi
Molekul Vol 18 No 1 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.1.5932

Urease has been utilized in the field of health and industry. Urease is commonly used in the form of free enzyme, so that the utilization is limited. Urease efficiency can be improved using immobilization enzyme. This research aimed to do the urease isolation, immobilization, and characterization from the winged bean seeds. This research was started by determining the amino-acid content of winged bean seeds using the Liquid chromatography-mass spectrometry (LCMS). The winged bean seeds were germinated and extracted. The obtained crude extract’s activity was determined using Nessler reagent and measured using UV-Vis spectrophotometer with the wavelength of 500 nm. The urease of winged bean seeds was immobilized using the alginate matrix. The optimization of urease-immobilized beads could be made through the variations of natrium alginate concentration and beads formation periods in solution CaCl2. Characterization free and immobilized urease were made using the variations of urea substrate concentration, pH, temperature, and also the repeated utilization of immobilized urease. Winged bean seeds are rich with essential amino acid, such as leucine, isoleucine, histidine, phenylalanine, and valine. The urease obtained from the winged bean seeds had the optimum activity in the germination period of 8 days. The urease immobilization showed the optimum condition in the natrium alginate concentration of 5% (w/v) and beads formation period in solution CaCl2 for 60 minutes. The characterization results of free urease and immobilization had the optimum condition at the urea substrate of 0.2 M, and pH 7. Free urease had the optimum temperature of 35 oC, while the immobilized urease had the optimum temperature of 40 oC. The immobilized urease had the utilization stability up to 5 times with the relative activity of 48%. The EDX analysis results showed that the alginate did not contain N, while alginate urease beads contained N as much as 12%.

Optimization and Characterization of Urease Immobilization from Red Lentil Seeds (Lens culinaris) Using Chitosan zusfahair, zusfahair; Ningsih, Dian Riana; Bilalodin, Bilalodin; Fatoni, Amin; Luthfia, Adilla; Purwati, Purwati; Muslihah, Niken Istikhari; Apriliadina, Inessa Putri
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.13134

ABSTRACT. Urease is an enzyme that plays a vital role in catalyzing the hydrolysis of urea into ammonia (NH3) and carbon dioxide (CO2). This study focuses on the isolation of urease from red lentil seeds, followed by its immobilization. The objective of this research is to optimize and characterize urease that has been immobilized using chitosan and activated with glutaraldehyde. Red lentil seeds were processed with a mortar and pestle at low temperatures (4 °C) to obtain a crude enzyme extract, which was then concentrated using 50% acetone (P50) prior to immobilization. The optimization process for P50 urease immobilization involved assessing various factors, including chitosan concentration, glutaraldehyde concentration, temperature, and the immersion duration in glutaraldehyde. The findings revealed that the optimal conditions for immobilizing P50 urease were achieved at a chitosan concentration of 0.75%, with a 2% glutaraldehyde soak at 25 °C for 2 hours, resulting in an enzyme activity of 7.042 U/g. The immobilized P50 urease demonstrated the ability to be reused up to 7 times while maintaining 51% of its initial activity. Scanning Electron Microscopy (SEM) analysis indicated morphological changes in the beads after the addition of glutaraldehyde and the enzyme, shifting from a rounded to an irregular shape. Additionally, Fourier Transform Infrared Spectroscopy (FTIR) analysis identified C-N and C=N peaks, confirming the successful incorporation of glutaraldehyde. Keywords: immobilization, red lentil seeds, glutaraldehyde, chitosan, urease

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